CLIP3 (NM_001199570) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CLIPR-59; CLIPR59; RSNL1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234405 representing NM_001199570 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTAAGACAGATCCTGCCCCGATGGCCCCGCCACCCCGAGGAGAGGAGGAAGAAGAGGAGGAGGAGG ATGAACCCGTCCCCGAGGCCCCCAGCCCCACCCAGGAGCGCCGGCAGAAGCCTGTTGTGCACCCCTCGGC ACCTGCCCCCCTCCCTAAGGACTACGCTTTCACCTTCTTCGATCCCAATGACCCGGCGTGCCAGGAGATC CTGTTTGACCCTCAGACCACCATCCCCGAGCTGTTTGCCATTGTGCGCCAGTGGGTGCCCCAAGTCCAGC ACAAGATAGACGTCATCGGCAATGAGATTCTGCGCCGAGGCTGCCATGTGAACGATCGTGACGGGCTGAC CGACATGACACTGCTCCACTATGCGTGCAAAGCTGGGGCCCACGGAGTCGGGGACCCCGCGGCAGCCGTG CGCCTCTCGCAGCAGCTGCTGGCGCTGGGCGCAGATGTGACGCTGCGCAGCCGCTGGACCAACATGAACG CGCTTCACTACGCGGCCTATTTTGATGTGCCCGACCTCGTGCGTGTGCTGCTGAAGGGTGCGAGGCCGCG AGTGGTGAACTCCACGTGCAGTGACTTCAACCACGGCTCAGCCCTGCACATCGCTGCTTCCAGCCTGTGC CTGGGCGCCGCCAAATGTTTGCTGGAGCACGGCGCCAACCCTGCGCTGAGGAATCGAAAAGGACAGGTGC CGGCGGAGGTGGTCCCAGATCCTATGGACATGTCCCTGGACAAGGCAGAGGCGGCACTGGTGGCCAAGGA GCTGCGGACGCTTCTGGAAGAGGCAGTGCCACTATCTTGCGCCCTCCCCAAGGTCACGCTACCCAACTAT GACAACGTCCCAGGCAATCTCATGCTTAGCGCACTGGGCTTGCGCCTGGGAGACCGCGTGCTGCTGGATG GCCAGAAGACGGGCACACTGCGGTTCTGTGGGACCACGGAGTTTGCCAGCGGCCAGTGGGTGGGCGTGGA GCTGGACGAACCTGAGGGCAAGAACGATGGCAGCGTTGGGGGCGTTCGGTACTTCATCTGCCCTCCCAAG CAGGGTCTCTTTGCCTCCGTGTCCAAGATCTCCAAGGCAGTGGACGCACCCCCCTCCTCTGTCACCTCCA CACCCCGGACCCCCCGGATGGACTTCTCCCGTGTCACCGGCAAAGGCCGCAGGGAACACAAAGGCAAGAA GAAGACCCCATCATCCCCATCTCTGGGCAGCTTGCAGCAGCGTGACGGGGCCAAGGCTGAGGTTGGAGAC CAGGTCCTTGTCGCGGGCCAGAAGCAGGGGATCGTGCGCTTCTACGGGAAGACAGACTTTGCCCCAGGTT ACTGGTATGGCATTGAGCTGGACCAGCCCACAGGCAAGCATGATGGCTCTGTCTTCGGTGTCCGGTACTT CACTTGCCCCCCGAGGCATGGGGTCTTCGCACCAGCATCCCGTATTCAGAGGATTGGCGGATCCACTGAT TCCCCCGGGGACAGCGTTGGAGCCAAAAAAGTGCATCAAGTGACAATGACGCAGCCCAAACGCACCTTCA CCACAGTCCGGACCCCAAAGGACATTGCATCAGAGAACTCCATTTCCAGGTTGCTGTTCTGCTGCTGGTT CCCCTGGATGCTGAGGGCGGAGATGCAGTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234405 representing NM_001199570 Red=Cloning site Green=Tags(s) MTKTDPAPMAPPPRGEEEEEEEEDEPVPEAPSPTQERRQKPVVHPSAPAPLPKDYAFTFFDPNDPACQEI LFDPQTTIPELFAIVRQWVPQVQHKIDVIGNEILRRGCHVNDRDGLTDMTLLHYACKAGAHGVGDPAAAV RLSQQLLALGADVTLRSRWTNMNALHYAAYFDVPDLVRVLLKGARPRVVNSTCSDFNHGSALHIAASSLC LGAAKCLLEHGANPALRNRKGQVPAEVVPDPMDMSLDKAEAALVAKELRTLLEEAVPLSCALPKVTLPNY DNVPGNLMLSALGLRLGDRVLLDGQKTGTLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGVRYFICPPK QGLFASVSKISKAVDAPPSSVTSTPRTPRMDFSRVTGKGRREHKGKKKTPSSPSLGSLQQRDGAKAEVGD QVLVAGQKQGIVRFYGKTDFAPGYWYGIELDQPTGKHDGSVFGVRYFTCPPRHGVFAPASRIQRIGGSTD SPGDSVGAKKVHQVTMTQPKRTFTTVRTPKDIASENSISRLLFCCWFPWMLRAEMQS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001199570
ORF Size	1641 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199570.2
RefSeq Size	3473 bp
RefSeq ORF	1644 bp
Locus ID	25999
UniProt ID	Q96DZ5
MW	59.6 kDa
Gene Summary	This gene encodes a member of the cytoplasmic linker protein 170 family. Members of this protein family contain a cytoskeleton-associated protein glycine-rich domain and mediate the interaction of microtubules with cellular organelles. The encoded protein plays a role in T cell apoptosis by facilitating the association of tubulin and the lipid raft ganglioside GD3. The encoded protein also functions as a scaffold protein mediating membrane localization of phosphorylated protein kinase B. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2010]

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