TARS1 (NM_001258438) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	TARS; ThrRS; TTD7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234841 representing NM_001258438 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTGAGGAGAAGGCCAGCAGTCCTTCAGGGAAGATGGGAGGCGAGGAGAAGCCGATTGGTGCTGGTG AAGAGAAGCAAAAGGAAGGAGGCAAAAAGAAGAACAAAGAAGGATCTGGAGATGGAGGTCGAGCTGAGTT GAATCCTTGGCCTGAATATATTTACACACGTCTTGAGATGTATAATATACTAAAAGCAGAACATGATTCC ATTCTGGCAGAAAAGGCAGAAAAAGATAGCAAGCCAATTAAAGTCACTTTGCCTGATGGTAAACAGGTTG ATGCGGAATCTTGGAAAACTACACCATATCAAATTGCCTGTGGAATTAGTCACACAGCTTCCTGCAAAAA CCTCTCTTCACTTGCATCTCTGCTTGCATCTGTGGCCATTCCCTCATCTGGAATGCCATGGCCTCCTCTT TTCTTCCTTCAAGGCCTGGCCGACAACACCGTTATTGCTAAAGTAAATAATGTTGTGTGGGACCTGGACC GCCCTCTGGAAGAAGATTGTACCTTGGAGCTTCTCAAGTTTGAGGATGAGGAAGCTCAGGCAGTGTATTG GCACTCTAGTGCTCACATAATGGGTGAAGCCATGGAAAGAGTCTATGGTGGATGTTTATGCTACGGTCCG CCAATAGAAAATGGATTCTATTATGACATGTACCTCGAAGAAGGGGGTGTGTCTAGCAATGATTTCTCTT CTCTGGAGGCTTTGTGTAAGAAAATCATTAAAGAAAAACAAGCTTTTGAAAGACTGGAAGTTAAGAAAGA AACTTTACTGGCAATGTTTAAGTACAACAAGTTCAAATGCCGGATATTGAATGAAAAGGTGAATACTCCA ACTACCACAGTCTATAGATGTGGCCCTTTGATAGATCTCTGCCGGGGTCCTCATGTTAGACACACGGGCA AAATTAAGGCTTTAAAAATACACAAAAATTCCTCCACGTACTGGGAAGGCAAAGCAGATATGGAGACTCT CCAGAGAATTTATGGCATTTCATTCCCAGATCCTAAAATGTTGAAAGAGTGGGAGAAGTTCCAAGAGGAA GCTAAAAACCGAGATCATAGGAAAATTGGCAGGGACCAAGAACTATATTTCTTTCATGAACTCAGCCCTG GAAGTTGCTTTTTTCTGCCAAAAGGAGCCTACATTTATAATGCACTTATTGAATTCATTAGGAGCGAATA TAGGAAAAGAGGATTCCAGGAGGTAGTCACCCCAAACATCTTCAACAGCCGACTCTGGATGACCTCGGGC CACTGGCAGCACTACAGCGAGAACATGTTCTCCTTTGAGGTGGAGAAGGAGCTGTTTGCCCTGAAACCCA TGAACTGCCCAGGACACTGCCTTATGTTTGATCATCGGCCAAGGTCCTGGCGAGAACTGCCTCTGCGGCT AGCTGATTTTGGGGTACTTCATAGGAACGAGCTGTCTGGAGCACTCACAGGACTCACCCGGGTACGAAGA TTCCAACAGGATGATGCTCACATATTCTGTGCCATGGAGCAGATTGAAGATGAAATAAAAGGTTGTTTGG ATTTTCTACGTACGGTATATAGCGTATTTGGATTTTCTTTTAAACTAAACCTTTCTACTCGCCCGGAAAA ATTCCTTGGAGATATCGAAGTATGGGATCAAGCTGAGAAACAACTTGAAAACAGTCTGAATGAATTTGGT GAAAAGTGGGAGTTAAACTCTGGAGATGGAGCTTTCTATGGCCCAAAGATTGACATACAGATTAAAGATG CGATTGGGCGGTACCACCAGTGTGCAACCATCCAGCTGGATTTCCAGTTGCCCATCAGATTTAATCTTAC TTATGTAAGCCATGATGGTGATGATAAGAAAAGGCCAGTGATTGTTCATCGAGCCATCTTGGGATCAGTG GAAAGAATGATTGCTATCCTCACAGAAAACTATGGGGGCAAATGGCCCTTTTGGCTGTCCCCTCGCCAGG TAATGGTAGTTCCAGTGGGACCAACCTGTGATGAATATGCCCAAAAGGTACGACAACAATTCCACGATGC CAAATTCATGGCAGACATTGATCTGGATCCAGGCTGTACATTGAATAAAAAGATTCGAAATGCACAGTTA GCACAGTATAACTTCATTTTAGTTGTTGGTGAAAAAGAGAAAATCAGTGGCACTGTTAATATCCGCACAA GAGACAATAAGGTCCACGGGGAACGCACCATTTCTGAAACTATCGAGCGGCTACAGCAGCTCAAAGAGTT CCGCAGCAAACAGGCAGAAGAAGAATTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234841 representing NM_001258438 Red=Cloning site Green=Tags(s) MFEEKASSPSGKMGGEEKPIGAGEEKQKEGGKKKNKEGSGDGGRAELNPWPEYIYTRLEMYNILKAEHDS ILAEKAEKDSKPIKVTLPDGKQVDAESWKTTPYQIACGISHTASCKNLSSLASLLASVAIPSSGMPWPPL FFLQGLADNTVIAKVNNVVWDLDRPLEEDCTLELLKFEDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGP PIENGFYYDMYLEEGGVSSNDFSSLEALCKKIIKEKQAFERLEVKKETLLAMFKYNKFKCRILNEKVNTP TTTVYRCGPLIDLCRGPHVRHTGKIKALKIHKNSSTYWEGKADMETLQRIYGISFPDPKMLKEWEKFQEE AKNRDHRKIGRDQELYFFHELSPGSCFFLPKGAYIYNALIEFIRSEYRKRGFQEVVTPNIFNSRLWMTSG HWQHYSENMFSFEVEKELFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRR FQQDDAHIFCAMEQIEDEIKGCLDFLRTVYSVFGFSFKLNLSTRPEKFLGDIEVWDQAEKQLENSLNEFG EKWELNSGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQLPIRFNLTYVSHDGDDKKRPVIVHRAILGSV ERMIAILTENYGGKWPFWLSPRQVMVVPVGPTCDEYAQKVRQQFHDAKFMADIDLDPGCTLNKKIRNAQL AQYNFILVVGEKEKISGTVNIRTRDNKVHGERTISETIERLQQLKEFRSKQAEEEF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001258438
ORF Size	2268 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001258438.1, NP_001245367.1
RefSeq Size	2974 bp
RefSeq ORF	2271 bp
Locus ID	6897
UniProt ID	P26639
Protein Families	Druggable Genome
Protein Pathways	Aminoacyl-tRNA biosynthesis
MW	87.3 kDa
Gene Summary	Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Threonyl-tRNA synthetase belongs to the class-II aminoacyl-tRNA synthetase family [provided by RefSeq, Jul 2008]

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