PITRM1 (NM_001242307) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MP1; PreP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235134 representing NM_001242307 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCGCTGCGGCGGGCGGCAGGGCCTGTGTGTGCTGAGGCGGCTGAGCGGCGGACATGCACACCACA GAGCGTGGCGATGGAACAGTAACCGGGCTTGTGAGAGGGCTCTGCAGTATAAACTAGGAGACAAGATCCA TGGATTCACCGTAAACCAGGTGACATCTGTTCCCGAGCTGTTCCTGACTGCAGTGAAGCTCACCCATGAT GACACAGGAGCCAGGTATTTACACCTGGCCAGAGAAGACACGAATAATCTGTTCAGCGTGCAGTTCCGTA CTACTCCCATGGACAGTACTGGTGTTCCTCACATTCTTGAGCATACCGTCCTTTGTGGGTCTCAGAAATA TCCGTGCAGAGACCCTTTCTTCAAAATGTTGAACCGGTCCCTCTCCACGTTCATGAACGCCTTCACAGCT AGTGATTATACTCTGTATCCATTTTCCACACAAAATCCCAAGGACTTTCAGAATCTCCTCTCGGTGTATT TGGATGCCACCTTTTTCCCATGTTTACGCGAGCTGGATTTCTGGCAGGAAGGATGGCGGCTGGAACATGA GAATCCGAGCGACCCCCAGACGCCCTTGGTCTTTAAAGGAGTCGTCTTTAATGAGATGAAGGGAGCGTTT ACAGACAATGAGAGGATATTCTCCCAGCACCTTCAGAACAGACTTCTTCCTGACCACACGTACTCAGTGG TCTCCGGGGGTGACCCACTGTGCATCCCGGAGCTTACATGGGAGCAGCTTAAGCAGTTTCATGCCACTCA CTATCACCCAAGCAATGCTAGGTTCTTCACGTACGGTAATTTTCCATTAGAACAGCATCTGAAACAAATT CACGAGGAAGCACTGAGCAAATTCCAGAAAATTGAACCAAGCACCGTGGTGCCAGCTCAGACACCCTGGG ACAAGCCTAGGGAATTCCAGATAACATGTGGCCCGGATTCATTTGCTACAGATCCCTCTAAACAAACAAC CATCAGCGTTAGCTTCCTCTTACCGGACATCACCGACACATTTGAAGCCTTCACATTAAGTCTTCTGTCT TCACTCTTGACTTCTGGGCCCAATTCTCCCTTTTACAAAGCCTTGATTGAATCTGGCCTTGGCACAGACT TTTCTCCTGATGTTGGATATAATGGCTACACGAGGGAGGCCTACTTTAGTGTCGGCCTCCAAGGGATTGC GGAGAAAGACATTGAGACCGTCAGAAGCCTCATAGACAGAACGATTGATGAAGTAGTTGAGAAAGGATTT GAAGATGATCGAATTGAGGCTTTACTTCATAAAATTGAAATACAGATGAAACATCAGTCTACCAGCTTTG GGCTGATGCTGACATCATACATAGCTTCTTGCTGGAACCATGATGGGGACCCTGTGGAGCTCTTGAAGTT GGGAAATCAGTTAGCTAAATTCAGACAGTGCCTGCAGGAAAATCCAAAATTTTTGCAAGAAAAAGTAAAA CAGTATTTTAAGAATAACCAGCATAAGCTGACTTTATCGATGAGGCCAGATGACAAGTATCACGAGAAGC AGGCACAGGTGGAAGCCACGAAGCTCAAGCAGAAGGTCGAGGCTCTGTCCCCCGGAGACAGGCAGCAGAT CTACGAGAAAGGTCTAGAATTACGGAGTCAACAAAGCAAACCTCAAGATGCCTCTTGTCTGCCAGCGTTG AAAGTTTCCGATATTGAACCCACCATACCTGTCACAGAGTTGGACGTGGTCCTGACAGCTGGAGATATCC CTGTTCAGTACTGCGCCCAGCCCACCAATGGCATGGTGTATTTCCGGGCCTTCTCCAGCCTGAACACACT CCCCGAGGAGCTGAGGCCCTATGTGCCCCTCTTCTGCAGCGTCCTCACCAAGCTGGGCTGCGGCCTTCTT GACTACCGGGAGCAGGCTCAGCAGATAGAATTGAAGACCGGAGGGATGAGTGCTTCTCCCCACGTGCTCC CCGACGACTCACACATGGACACCTACGAGCAGGTAGGTGTGCTTTTCTCCTCTCTCTGCCTGGATCGAAA CCTGCCAGACATGATGCAGCTATGGAGTGAAATATTTAACAACCCGTGCTTTGAAGAAGAGGAGCACTTC AAGGTGCTGGTGAAGATGACCGCCCAGGAGCTCGCCAATGGAATTCCTGACTCTGGGCACCTGTACGCAT CCATCAGGGCAGGCCGGACCCTCACGCCCGCAGGGGACCTGCAGGAGACCTTCAGCGGGATGGATCAGGT GCGGCTGATGAAGAGGATTGCAGAAATGACAGATATCAAACCCATCCTGAGGAAGCTCCCGCGTATCAAG AAACACTTGTTAAATGGTGATAATATGAGGTGTTCAGTGAATGCGACTCCTCAGCAGATGCCTCAGACAG AAAAAGCGGTCGAAGACTTCCTTAGAAGCATCGGTCGGAGTAAAAAGGAACGGAGGCCTGTGCGCCCACA CACGGTCGAGAAACCTGTGCCCAGCAGCTCTGGTGGAGATGCCCACGTTCCCCATGGCTCCCAGGTCATT AGGAAGCTGGTCATGGAACCCACCTTCAAGCCCTGGCAGATGAAGACTCACTTCCTGATGCCCTTCCCGG TGAATTACGTGGGTGAATGCATCCGAACTGTCCCCTACACGGACCCAGATCATGCCAGTCTTAAAATCCT TGCACGTTTGATGACTGCCAAATTCTTGCATACAGAAATTCGAGAAAAAGGCGGTGCTTATGGTGGAGGC GCAAAACTCAGCCACAATGGGATTTTCACCCTTTACTCTTACAGGGACCCAAATACAATAGAGACGCTCC AGTCTTTTGGGAAGGCTGTCGACTGGGCTAAGTCTGGAAAATTCACACAGCAAGACATCGACGAAGCCAA ACTTTCTGTCTTCTCAACCGTAGATGCTCCTGTCGCTCCTTCAGACAAAGGAATGGACCACTTCTTGTAC GGCCTCTCGGATGAGATGAAGCAGGCCCACAGAGAGCAGCTCTTTGCTGTCAGCCACGACAAGCTCCTGG CCGTGAGCGATAGATACCTCGGCACTGGGAAGAGCACACACGGCCTGGCCATCCTCGGACCCGAGAACCC GAAAATTGCCAAGGACCCATCCTGGATCATCCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235134 representing NM_001242307 Red=Cloning site Green=Tags(s) MWRCGGRQGLCVLRRLSGGHAHHRAWRWNSNRACERALQYKLGDKIHGFTVNQVTSVPELFLTAVKLTHD DTGARYLHLAREDTNNLFSVQFRTTPMDSTGVPHILEHTVLCGSQKYPCRDPFFKMLNRSLSTFMNAFTA SDYTLYPFSTQNPKDFQNLLSVYLDATFFPCLRELDFWQEGWRLEHENPSDPQTPLVFKGVVFNEMKGAF TDNERIFSQHLQNRLLPDHTYSVVSGGDPLCIPELTWEQLKQFHATHYHPSNARFFTYGNFPLEQHLKQI HEEALSKFQKIEPSTVVPAQTPWDKPREFQITCGPDSFATDPSKQTTISVSFLLPDITDTFEAFTLSLLS SLLTSGPNSPFYKALIESGLGTDFSPDVGYNGYTREAYFSVGLQGIAEKDIETVRSLIDRTIDEVVEKGF EDDRIEALLHKIEIQMKHQSTSFGLMLTSYIASCWNHDGDPVELLKLGNQLAKFRQCLQENPKFLQEKVK QYFKNNQHKLTLSMRPDDKYHEKQAQVEATKLKQKVEALSPGDRQQIYEKGLELRSQQSKPQDASCLPAL KVSDIEPTIPVTELDVVLTAGDIPVQYCAQPTNGMVYFRAFSSLNTLPEELRPYVPLFCSVLTKLGCGLL DYREQAQQIELKTGGMSASPHVLPDDSHMDTYEQVGVLFSSLCLDRNLPDMMQLWSEIFNNPCFEEEEHF KVLVKMTAQELANGIPDSGHLYASIRAGRTLTPAGDLQETFSGMDQVRLMKRIAEMTDIKPILRKLPRIK KHLLNGDNMRCSVNATPQQMPQTEKAVEDFLRSIGRSKKERRPVRPHTVEKPVPSSSGGDAHVPHGSQVI RKLVMEPTFKPWQMKTHFLMPFPVNYVGECIRTVPYTDPDHASLKILARLMTAKFLHTEIREKGGAYGGG AKLSHNGIFTLYSYRDPNTIETLQSFGKAVDWAKSGKFTQQDIDEAKLSVFSTVDAPVAPSDKGMDHFLY GLSDEMKQAHREQLFAVSHDKLLAVSDRYLGTGKSTHGLAILGPENPKIAKDPSWIIQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001242307
ORF Size	3114 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242307.1, NP_001229236.1
RefSeq Size	3508 bp
RefSeq ORF	3117 bp
Locus ID	10531
UniProt ID	Q5JRX3
Protein Families	Druggable Genome, Protease
MW	118 kDa
Gene Summary	The protein encoded by this gene is an ATP-dependent metalloprotease that degrades post-cleavage mitochondrial transit peptides. The encoded protein binds zinc and can also degrade amyloid beta A4 protein, suggesting a possible role in Alzheimer's disease. [provided by RefSeq, Dec 2016]

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