ALDH1A3 (NM_001293815) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ALDH1A6; ALDH6; MCOP8; RALDH3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238016 representing NM_001293815 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCGCTAACGGGGCCGTGGAAAACGGGCAGCCGGACAGGAAGCCGCCGGCCCTGCCGCGCCCCA TCCGCAACCTGGAGGTCAAGTTCACCAAGATATTTATCAACAATGAATGGCACGAATCCAAGAGTGGGAA AAAGTTTGCTACATGTAACCCTTCAACTCGGGAGCAAATATGTGAAGTGGAAGAAGGAGATAAGCCCGAC GTGGACAAGGCTGTGGAGGCTGCACAGGTTGCCTTCCAGAGGGGCTCGCCATGGCGCCGGCTGGATGCCC TGAGTCGTGGGCGGCTGCTGCACCAGCTGGCTGACCTGGTGGAGAGGGACCGCGCCACCTTGGCCGCCGG GTTCCCTCCAGGAGTGGTGAACATTGTGCCAGGATTCGGGCCCACAGTGGGAGCAGCAATTTCTTCTCAC CCTCAGATCAACAAGATCGCCTTCACCGGCTCCACAGAGGTTGGAAAACTGGTTAAAGAAGCTGCGTCCC GGAGCAATCTGAAGCGGGTGACGCTGGAGCTGGGGGGGAAGAACCCCTGCATCGTGTGTGCGGACGCTGA CTTGGACTTGGCAGTGGAGTGTGCCCATCAGGGAGTGTTCTTCAACCAAGGCCAGTGTTGCACGGCAGCC TCCAGGGTGTTCGTGGAGGAGCAGGTCTACTCTGAGTTTGTCAGGCGGAGCGTGGAGTATGCCAAGAAAC GGCCCGTGGGAGACCCCTTCGATGTCAAAACAGAACAGGGGCCTCAGATTGATCAAAAGCAGTTCGACAA AATCTTAGAGCTGATCGAGAGTGGGAAGAAGGAAGGGGCCAAGCTGGAATGCGGGGGCTCAGCCATGGAA GACAAGGGGCTCTTCATCAAACCCACTGTCTTCTCAGAAGTCACAGACAACATGCGGATTGCCAAAGAGG AGATTTTCGGGCCAGTGCAACCAATACTGAAGTTCAAAAGTATCGAAGAAGTGATAAAAAGAGCGAATAG CACCGACTATGGACTCACAGCAGCCGTGTTCACAAAAAATCTCGACAAAGCCCTGAAGTTGGCTTCTGCC TTAGAGTCTGGAACGGTCTGGATCAACTGCTACAACGCCCTCTATGCACAGGCTCCATTTGGTGGCTTTA AAATGTCAGGAAATGGCAGAGAACTAGGTGAATACGCTTTGGCCGAATACACAGAAGTGAAAACTGTCAC CATCAAACTTGGCGACAAGAACCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238016 representing NM_001293815 Red=Cloning site Green=Tags(s) MATANGAVENGQPDRKPPALPRPIRNLEVKFTKIFINNEWHESKSGKKFATCNPSTREQICEVEEGDKPD VDKAVEAAQVAFQRGSPWRRLDALSRGRLLHQLADLVERDRATLAAGFPPGVVNIVPGFGPTVGAAISSH PQINKIAFTGSTEVGKLVKEAASRSNLKRVTLELGGKNPCIVCADADLDLAVECAHQGVFFNQGQCCTAA SRVFVEEQVYSEFVRRSVEYAKKRPVGDPFDVKTEQGPQIDQKQFDKILELIESGKKEGAKLECGGSAME DKGLFIKPTVFSEVTDNMRIAKEEIFGPVQPILKFKSIEEVIKRANSTDYGLTAAVFTKNLDKALKLASA LESGTVWINCYNALYAQAPFGGFKMSGNGRELGEYALAEYTEVKTVTIKLGDKNP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001293815
ORF Size	1215 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001293815.2
RefSeq Size	3301 bp
RefSeq ORF	1218 bp
Locus ID	220
UniProt ID	P47895
Protein Families	Druggable Genome
Protein Pathways	Drug metabolism - cytochrome P450, Glycolysis / Gluconeogenesis, Histidine metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Phenylalanine metabolism, Tyrosine metabolism
MW	44.7 kDa
Gene Summary	This gene encodes an aldehyde dehydrogenase enzyme that uses retinal as a substrate. Mutations in this gene have been associated with microphthalmia, isolated 8, and expression changes have also been detected in tumor cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]

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