FMO1 (NM_001282694) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238406 representing NM_001282694 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAAGCGAGTTGCCATTGTGGGAGCTGGGGTCAGCGGCCTGGCCTCCATCAAGTGCTGTCTGGAAG AAGGACTGGAGCCCACCTGCTTTGAGAGGAGCGATGACCTTGGGGGGCTGTGGAGATTCACCACCAAAGT CTGCAGTGTAACAAAATGCTCAGATTCTGCTGTCTCTGGCCAATGGGAGGTGGTCACTATGCATGAAGAG AAGCAAGAGTCAGCCATCTTTGATGCTGTCATGGTCTGCACTGGCTTTCTTACTAATCCTTATTTGCCAC TGGATTCCTTTCCAGGTATTAATGCCTTTAAAGGCCAGTACTTTCATAGCCGGCAATATAAGCATCCAGA TATATTTAAGGACAAGAGAGTCCTTGTGATTGGAATGGGAAATTCTGGCACAGACATTGCTGTGGAGGCC AGCCACCTGGCGGAAAAGGTGTTCCTCAGCACCACCGGAGGGGGATGGGTGATCAGCCGAATCTTTGACT CGGGCTACCCATGGGACATGGTGTTCATGACACGCTTTCAGAACATGTTGAGAAATTCCCTCCCAACCCC AATTGTGACTTGGTTGATGGAGCGAAAGATAAACAACTGGCTCAATCATGCAAATTACGGCTTAATACCA GAAGACAGGACTCAGCTGAAAGAGTTTGTGCTAAATGATGAGCTCCCAGGACGCATCATCACTGGGAAAG TGTTCATCAGGCCAAGCATAAAAGAGGTAAAGGAAAACTCTGTCATATTTAACAATACTTCAAAGGAAGA GCCTATTGACATCATTGTCTTTGCCACTGGATACACATTTGCTTTCCCCTTCCTTGATGAGTCTGTAGTG AAAGTTGAAGATGGCCAGGCCTCACTGTACAAGTATATCTTCCCTGCACATCTGCAAAAGCCAACCCTGG CCATTATTGGCCTCATCAAACCCTTGGGCTCCATGATACCTACAGGAGAAACACAAGCTCGGTGGGCTGT TCGAGTCCTGAAAGGTGTAAATAAGTTACCACCACCAAGTGTCATGATAGAGGAAATTAATGCAAGGAAA GAAAACAAGCCCAGTTGGTTTGGCTTGTGCTACTGCAAGGCTTTACAATCAGATTATATCACATACATAG ATGAACTCCTGACCTATATCAATGCAAAACCCAACCTGTTCTCTATGCTCCTAACGGATCCACATCTGGC TCTGACCGTCTTCTTTGGCCCATGCTCACCATACCAGTTCCGCTTGACTGGCCCAGGAAAATGGGAAGGA GCCAGAAATGCCATCATGACCCAGTGGGACCGAACATTCAAGGTCATCAAAGCTCGAGTTGTACAAGAGT CTCCATCTCCCTTTGAAAGTTTTCTTAAAGTCTTTAGCTTTCTGGCTTTGCTTGTGGCTATTTTTCTGAT TTTCCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238406 representing NM_001282694 Red=Cloning site Green=Tags(s) MAKRVAIVGAGVSGLASIKCCLEEGLEPTCFERSDDLGGLWRFTTKVCSVTKCSDSAVSGQWEVVTMHEE KQESAIFDAVMVCTGFLTNPYLPLDSFPGINAFKGQYFHSRQYKHPDIFKDKRVLVIGMGNSGTDIAVEA SHLAEKVFLSTTGGGWVISRIFDSGYPWDMVFMTRFQNMLRNSLPTPIVTWLMERKINNWLNHANYGLIP EDRTQLKEFVLNDELPGRIITGKVFIRPSIKEVKENSVIFNNTSKEEPIDIIVFATGYTFAFPFLDESVV KVEDGQASLYKYIFPAHLQKPTLAIIGLIKPLGSMIPTGETQARWAVRVLKGVNKLPPPSVMIEEINARK ENKPSWFGLCYCKALQSDYITYIDELLTYINAKPNLFSMLLTDPHLALTVFFGPCSPYQFRLTGPGKWEG ARNAIMTQWDRTFKVIKARVVQESPSPFESFLKVFSFLALLVAIFLIFL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282694
ORF Size	1407 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282694.1, NP_001269623.1
RefSeq Size	2003 bp
RefSeq ORF	1410 bp
Locus ID	2326
UniProt ID	Q01740
Protein Families	Druggable Genome
Protein Pathways	Drug metabolism - cytochrome P450
MW	53.3 kDa
Gene Summary	Metabolic N-oxidation of the diet-derived amino-trimethylamine (TMA) is mediated by flavin-containing monooxygenase and is subject to an inherited FMO3 polymorphism in man resulting in a small subpopulation with reduced TMA N-oxidation capacity resulting in fish odor syndrome Trimethylaminuria. Three forms of the enzyme, FMO1 found in fetal liver, FMO2 found in adult liver, and FMO3 are encoded by genes clustered in the 1q23-q25 region. Flavin-containing monooxygenases are NADPH-dependent flavoenzymes that catalyzes the oxidation of soft nucleophilic heteroatom centers in drugs, pesticides, and xenobiotics. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]

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