SLC2A14 (NM_001286233) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	GLUT14; SLC2A3P3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238700 representing NM_001286233 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTTCACAATGGTGGCCATGTGTCTGGAATTGGTGGGTTCTTGGTCTCACTGACTTCAAGAATGA AGCCGCACACCCTCGCAGTCACCCCAGCTCTGATCTTTGCCATCACAGTTGCTACAATCGGCTCTTTCCA GTTTGGCTACAACACTGGGGTCATCAATGCTCCTGAGACGATCATAAAGGAATTTATCAATAAAACTTTG ACGGACAAGGCAAATGCCCCTCCCTCTGAGGTGCTGCTCACGAATCTCTGGTCCTTGTCTGTGGCCATAT TTTCCGTCGGGGGTATGATCGGCTCCTTTTCCGTCGGACTCTTTGTTAACCGCTTTGGCAGGCGCAATTC AATGCTGATTGTCAACCTGTTGGCTGCCACTGGTGGCTGCCTTATGGGACTGTGTAAAATAGCTGAGTCA GTTGAAATGCTGATCCTGGGCCGCTTGGTTATTGGCCTCTTCTGCGGACTCTGCACAGGTTTTGTGCCCA TGTACATTGGAGAGATCTCGCCTACTGCCCTGAGGGGTGCCTTTGGCACTCTCAACCAGCTGGGCATAGT TATTGGAATTCTGGTGGCCCAGATCTTTGGTCTGGAACTCATCCTTGGGTCTGAAGAGCTATGGCCGGTG CTATTAGGCTTTACCATCCTTCCAGCTATCCTGCAAAGTGCAGCCCTTCCATGTTGCCCTGAAAGTCCCA GATTTTTGCTCATTAACAGAAAAAAAGAGGAGAATGCTACGCGGATCCTCCAGCGGTTGTGGGGCACCCA GGATGTATCCCAAGACATCCAGGAGATGAAAGATGAGAGTGCAAGGATGTCACAAGAAAAGCAAGTCACC GTGCTGGAGCTCTTTAGAGTGTCCAGCTACCGACAGCCCATCATCATTTCCATTGTGCTCCAGCTCTCTC AGCAGCTCTCTGGGATCAATGCTGTGTTCTATTACTCAACAGGAATCTTCAAGGATGCAGGTGTTCAACA GCCCATCTATGCCACCATCAGCGCGGGTGTGGTTAATACTATCTTCACTTTACTTTCTCTATTTCTGGTG GAAAGGGCAGGAAGAAGGACTCTGCATATGATAGGCCTTGGAGGGATGGCTTTTTGTTCCACGCTCATGA CTGTTTCTTTGTTATTAAAGAATCACTATAATGGGATGAGCTTTGTCTGTATTGGGGCTATCTTGGTCTT TGTGGCCTGTTTTGAAATTGGACCAGGCCCCATTCCCTGGTTTATTGTGGCCGAACTCTTCAGCCAGGGC CCCCGCCCAGCTGCGATGGCAGTGGCCGGCTGCTCCAACTGGACCTCCAACTTCCTAGTCGGATTGCTCT TCCCCTCTGCTGCTTACTATTTAGGAGCCTACGTTTTTATTATCTTCACCGGCTTCCTCATTACCTTCTT GGCCTTTACCTTCTTCAAAGTCCCTGAGACCCGTGGCAGGACTTTTGAGGATATCACACGGGCCTTTGAA GGGCAGGCACACGGTGCAGATAGATCTGGGAAGGACGGCGTCATGGGGATGAACAGCATCGAGCCTGCTA AGGAGACCACCACCAATGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238700 representing NM_001286233 Red=Cloning site Green=Tags(s) MEFHNGGHVSGIGGFLVSLTSRMKPHTLAVTPALIFAITVATIGSFQFGYNTGVINAPETIIKEFINKTL TDKANAPPSEVLLTNLWSLSVAIFSVGGMIGSFSVGLFVNRFGRRNSMLIVNLLAATGGCLMGLCKIAES VEMLILGRLVIGLFCGLCTGFVPMYIGEISPTALRGAFGTLNQLGIVIGILVAQIFGLELILGSEELWPV LLGFTILPAILQSAALPCCPESPRFLLINRKKEENATRILQRLWGTQDVSQDIQEMKDESARMSQEKQVT VLELFRVSSYRQPIIISIVLQLSQQLSGINAVFYYSTGIFKDAGVQQPIYATISAGVVNTIFTLLSLFLV ERAGRRTLHMIGLGGMAFCSTLMTVSLLLKNHYNGMSFVCIGAILVFVACFEIGPGPIPWFIVAELFSQG PRPAAMAVAGCSNWTSNFLVGLLFPSAAYYLGAYVFIIFTGFLITFLAFTFFKVPETRGRTFEDITRAFE GQAHGADRSGKDGVMGMNSIEPAKETTTNV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286233
ORF Size	1560 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286233.2
RefSeq Size	4368 bp
RefSeq ORF	1563 bp
Locus ID	144195
UniProt ID	Q8TDB8
Protein Families	Transmembrane
MW	56.3 kDa
Gene Summary	Members of the glucose transporter (GLUT) family, including SLC2A14, are highly conserved integral membrane proteins that transport hexoses such as glucose and fructose into all mammalian cells. GLUTs show tissue and cell-type specific expression (Wu and Freeze, 2002 [PubMed 12504846]).[supplied by OMIM, Mar 2008]

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