MPP2 (NM_001278381) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	DLG2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238867 representing NM_001278381 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGTTGCCGCCACCAACTCTGAAACTGCCATGCAGCAAGTCCTGGACAACTTGGGATCCCTCCCCA GTGCCACGGGGGCTGCAGAGCTGGACCTGATCTTCCTTCGAGGCATTATGGAAAGTCCCATAGTAAGATC CCTGGCCAAGGCCCATGAGAGGCTGGAGGAGACGAAGCTGGAGGCCGTGAGAGACAACAACCTGGAGCTG GTGCAGGAGATCCTGCGGGACCTGGCGCAGCTGGCTGAGCAGAGCAGCACAGCCGCCGAGCTGGCCCACA TCCTCCAGGAGCCCCACTTCCAGTCCCTCCTGGAGACGCACGACTCTGTGGCCTCAAAGACCTATGAGAC ACCACCCCCCAGCCCTGGCCTGGACCCTACATTCAGCAACCAGCCTGTACCTCCCGATGCTGTGCGCATG GTGGGCATCCGCAAGACAGCCGGAGAACATCTGGGTGTAACGTTCCGCGTGGAGGGCGGCGAGCTGGTGA TCGCGCGCATTCTGCATGGGGGCATGGTGGCTCAACAAGGCCTGCTGCATGTGGGTGACATCATCAAGGA GGTGAACGGGCAGCCAGTGGGCAGTGACCCCCGCGCACTGCAGGAGCTCCTGCGCAATGCCAGTGGCAGT GTCATCCTCAAGATCCTGCCCAGCTACCAGGAGCCCCATCTGCCCCGCCAGGTATTTGTGAAATGTCACT TTGACTATGACCCGGCCCGAGACAGCCTCATCCCCTGCAAGGAAGCAGGCCTGCGCTTCAACGCCGGGGA CTTGCTCCAGATCGTAAACCAGGATGATGCCAACTGGTGGCAGGCATGCCATGTCGAAGGGGGCAGTGCT GGGCTCATTCCCAGCCAGCTGCTGGAGGAGAAGCGGAAAGCATTTGTCAAGAGGGACCTGGAGCTGACAC CAAACTCAGGGACCCTATGCGGCAGCCTTTCAGGAAAGAAAAAGAAGCGAATGATGTATTTGACCACCAA GAATGCAGAGTTTGACCGTCATGAGCTGCTCATTTATGAGGAGGTGGCCCGCATGCCCCCGTTCCGCCGG AAAACCCTGGTACTGATTGGGGCTCAGGGCGTGGGACGGCGCAGCCTGAAGAACAAGCTCATCATGTGGG ATCCAGATCGCTATGGCACCACGGTGCCCTACACCTCCCGGCGGCCGAAAGACTCAGAGCGGGAAGGTCA GGGTTACAGCTTTGTGTCCCGTGGGGAGATGGAGGCTGACGTCCGTGCTGGGCGCTACCTGGAGCATGGC GAATACGAGGGCAACCTGTATGGCACACGTATTGACTCCATCCGGGGCGTGGTCGCTGCTGGGAAGGTGT GCGTGCTGGATGTCAACCCCCAGGCGGTGAAGGTGCTACGAACGGCCGAGTTTGTCCCTTACGTGGTGTT CATCGAGGCCCCAGACTTCGAGACCCTGCGGGCCATGAACAGGGCTGCGCTGGAGAGTGGAATATCCACC AAGCAGCTCACGGAGGCGGACCTGAGACGGACAGTGGAGGAGAGCAGCCGCATCCAGCGGGGCTACGGGC ACTACTTTGACCTCTGCCTGGTCAATAGCAACCTGGAGAGGACCTTCCGCGAGCTCCAGACAGCCATGGA GAAGCTACGGACAGAGCCCCAGTGGGTGCCTGTCAGCTGGGTGTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238867 representing NM_001278381 Red=Cloning site Green=Tags(s) MPVAATNSETAMQQVLDNLGSLPSATGAAELDLIFLRGIMESPIVRSLAKAHERLEETKLEAVRDNNLEL VQEILRDLAQLAEQSSTAAELAHILQEPHFQSLLETHDSVASKTYETPPPSPGLDPTFSNQPVPPDAVRM VGIRKTAGEHLGVTFRVEGGELVIARILHGGMVAQQGLLHVGDIIKEVNGQPVGSDPRALQELLRNASGS VILKILPSYQEPHLPRQVFVKCHFDYDPARDSLIPCKEAGLRFNAGDLLQIVNQDDANWWQACHVEGGSA GLIPSQLLEEKRKAFVKRDLELTPNSGTLCGSLSGKKKKRMMYLTTKNAEFDRHELLIYEEVARMPPFRR KTLVLIGAQGVGRRSLKNKLIMWDPDRYGTTVPYTSRRPKDSEREGQGYSFVSRGEMEADVRAGRYLEHG EYEGNLYGTRIDSIRGVVAAGKVCVLDVNPQAVKVLRTAEFVPYVVFIEAPDFETLRAMNRAALESGIST KQLTEADLRRTVEESSRIQRGYGHYFDLCLVNSNLERTFRELQTAMEKLRTEPQWVPVSWVY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278381
ORF Size	1656 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278381.1, NP_001265310.1
RefSeq Size	4978 bp
RefSeq ORF	1659 bp
Locus ID	4355
UniProt ID	Q14168
Protein Families	Druggable Genome
MW	62 kDa
Gene Summary	Palmitoylated membrane protein 2 is a member of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. Palmitoylated membrane protein 2 contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction. [provided by RefSeq, Jul 2008]

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