ZNF43 (NM_001256653) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HTF6; KOX27; ZNF39L1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239625 representing NM_001256653 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGGACATCCTGGAAGCTGGGAAATGGGACCATTGACATTTATGGATGTGGCCATAGAATTCTGTC TGGAGGAGTGGCAATGCCTGGACATTGCACAGCAGAATTTATATAGGAATGTGATGTTAGAGAACTACAG AAACCTGGTCTTCCTGGGTATTGCTGTCTCTAAGCCAGACCTGATCACCTGTCTGGAGCAAGAAAAAGAG CCTTGGGAGCCTATGAGGAGACATGAAATGGTAGCCAAACCCCCAGTTATGTGTTCTCATTTTACCCAAG ACTTTTGGCCAGAGCAGCATATAAAAGATCCTTTCCAAAAAGCGACACTGAGAAGATATAAAAACTGTGA ACATAAAAATGTACATTTAAAAAAAGACCATAAAAGTGTGGATGAGTGTAAGGTGCACAGAGGAGGTTAT AATGGATTTAACCAATGTTTGCCAGCTACCCAGAGCAAAATATTTCTATTTGATAAATGTGTGAAAGCCT TTCATAAATTTTCAAATTCAAACAGACATAAGATAAGCCATACTGAAAAAAAACTTTTCAAATGCAAAGA ATGTGGCAAATCATTTTGCATGCTTCCACATCTAGCTCAACATAAAATAATTCATACCAGAGTGAATTTC TGCAAATGTGAAAAATGTGGAAAAGCTTTTAACTGCCCTTCAATCATCACTAAACATAAGAGAATTAATA CTGGAGAGAAACCCTACACATGTGAAGAATGTGGCAAAGTCTTTAATTGGTCCTCACGCCTTACTACACA TAAAAAAAATTATACTAGATACAAACTCTACAAATGTGAAGAATGTGGCAAAGCTTTTAACAAGTCCTCA ATCCTTACTACCCATAAGATAATTCGCACTGGAGAGAAATTCTACAAATGTAAAGAATGTGCCAAAGCTT TTAACCAATCCTCAAACCTTACTGAACATAAGAAAATTCATCCTGGAGAGAAACCTTACAAATGTGAAGA ATGTGGCAAAGCCTTTAACTGGCCCTCAACTCTTACTAAACATAAGAGAATTCATACTGGAGAGAAACCC TACACATGTGAAGAATGTGGCAAAGCCTTTAACCAGTTCTCAAACCTTACTACACATAAGAGAATCCATA CTGCAGAGAAATTCTATAAATGTACAGAATGTGGTGAAGCTTTTAGCCGGTCCTCAAACCTTACTAAACA TAAGAAAATTCATACTGAAAAGAAACCCTACAAATGTGAAGAATGTGGCAAAGCTTTTAAGTGGTCCTCA AAGCTTACTGAACATAAGTTAACTCATACTGGAGAGAAACCCTACAAATGTGAAGAATGTGGCAAAGCCT TTAACTGGCCCTCAACCCTTACTAAACATAACAGAATTCATACTGGAGAGAAACCCTACAAATGTGAAGT ATGTGGCAAAGCCTTTAACCAGTTCTCAAACCTTACTACACATAAGAGAATTCATACTGCAGAAAAACCG TACAAATGTGAAGAATGTGGCAAAGCTTTTAGCCGGTCCTCAAACCTTACTAAACATAAGAAAATTCACA TTGAAAAGAAACCCTACAAATGTGAAGAATGTGGCAAAGCTTTTAAGTGGTCCTCAAAGCTTACTGAACA TAAGATAACTCATACTGGAGAGAAACCCTACAAATGTGAAGAATGTGGCAAAGCTTTTAACCATTTCTCA ATCCTTACCAAACATAAGAGGATTCATACTGGAGAGAAACCCTACAAGTGTGAAGAATGTGGCAAAGCTT TTACCCAATCCTCAAACCTTACTACACATAAGAAAATTCATACTGGAGAGAAATTCTACAAATGTGAAGA ATGTGGCAAAGCTTTTACCCAATCTTCAAACCTTACTACACATAAAAAAATTCATACTGGAGGAAAACCC TACAAATGTGAAGAATGTGGCAAAGCTTTTAACCAGTTCTCAACTCTTACTAAACATAAGATAATTCACA CTGAGGAGAAACCCTACAAATGTGAAGAATGTGGCAAAGCCTTTAAGTGGTCCTCAACCCTTACTAAACA TAAGATAATTCATACTGGAGAGAAACCCTACAAATGTGAAGAATGTGGCAAAGCTTTTAAACTGTCCTCA ACCCTTTCTACACATAAGATTATTCATACTGGAGAGAAACCCTACAAATGTGAAAAATGTGGCAAAGCTT TTAACCGATCCTCAAACCTTATTGAACATAAGAAAATTCATACTGGAGAGCAACCCTACAAATGTGAAGA ATGTGGCAAAGCATTTAACTATTCCTCACACCTTAATACACATAAGAGAATTCATACTAAAGAGCAACCC TACAAATGTAAAGAATGTGGCAAAGCTTTCAACCAATATTCAAACCTTACTACACATAACAAAATTCATA CTGGAGAGAAACTCTACAAACCTGAAGATGTGACAGTGATTTTGACAACACCTCAAACTTTTTCAAACAT AAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239625 representing NM_001256653 Red=Cloning site Green=Tags(s) MPGHPGSWEMGPLTFMDVAIEFCLEEWQCLDIAQQNLYRNVMLENYRNLVFLGIAVSKPDLITCLEQEKE PWEPMRRHEMVAKPPVMCSHFTQDFWPEQHIKDPFQKATLRRYKNCEHKNVHLKKDHKSVDECKVHRGGY NGFNQCLPATQSKIFLFDKCVKAFHKFSNSNRHKISHTEKKLFKCKECGKSFCMLPHLAQHKIIHTRVNF CKCEKCGKAFNCPSIITKHKRINTGEKPYTCEECGKVFNWSSRLTTHKKNYTRYKLYKCEECGKAFNKSS ILTTHKIIRTGEKFYKCKECAKAFNQSSNLTEHKKIHPGEKPYKCEECGKAFNWPSTLTKHKRIHTGEKP YTCEECGKAFNQFSNLTTHKRIHTAEKFYKCTECGEAFSRSSNLTKHKKIHTEKKPYKCEECGKAFKWSS KLTEHKLTHTGEKPYKCEECGKAFNWPSTLTKHNRIHTGEKPYKCEVCGKAFNQFSNLTTHKRIHTAEKP YKCEECGKAFSRSSNLTKHKKIHIEKKPYKCEECGKAFKWSSKLTEHKITHTGEKPYKCEECGKAFNHFS ILTKHKRIHTGEKPYKCEECGKAFTQSSNLTTHKKIHTGEKFYKCEECGKAFTQSSNLTTHKKIHTGGKP YKCEECGKAFNQFSTLTKHKIIHTEEKPYKCEECGKAFKWSSTLTKHKIIHTGEKPYKCEECGKAFKLSS TLSTHKIIHTGEKPYKCEKCGKAFNRSSNLIEHKKIHTGEQPYKCEECGKAFNYSSHLNTHKRIHTKEQP YKCKECGKAFNQYSNLTTHNKIHTGEKLYKPEDVTVILTTPQTFSNIK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001256653
ORF Size	2454 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256653.1, NP_001243582.1
RefSeq Size	5249 bp
RefSeq ORF	2457 bp
Locus ID	7594
Protein Families	Transcription Factors
MW	95.6 kDa
Gene Summary	This gene belongs to the C2H2-type zinc finger gene family. The zinc finger proteins are involved in gene regulation and development, and are quite conserved throughout evolution. Like this gene product, a third of the zinc finger proteins containing C2H2 fingers also contain the KRAB domain, which has been found to be involved in protein-protein interactions. [provided by RefSeq, Jul 2008]

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