STAG3 (NM_001282718) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240016 representing NM_001282718 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTCCCCGTTGCAAAGAGCTGTGGGAGATACCAAGAGGGCCTTGTCTGCATCTTCTAGTTCCTCTG CCAGTCTACCCTTTGATGACAGGGACTCAAACCATACCTCAGAGGGGAATGGCGACTCTTTGTTAGCTGA TGAAGACACTGACTTTGAAGACAGCTTGAATCGCAATGTGAAGAAGAGAGCAGCAAAACGACCACCGAAA ACAACACCGGTGGCAAAACATCCAAAGAAAGGGTCCCGAGTGGTACATCGTCATAGCCGGAAACAGTCAG AGCCACCAGCCAATGATCTTTTCAATGCTGTGAAAGCCGCCAAAAGTGACATGCAGGACTCGGGGGACTA CCCTCTCATAGCTCCAGGTCCATCCTGGAAGAAGTTCCAGGGCAGCTTCTGTGAATTTGTGAGGACATTG GTCTGTCAGTGCCAGTACAGCCTCCTCTATGATGGCTTCCCTATGGACGACCTCATCTCCCTGCTCACTG GCCTCTCAGACTCACAAGTCCGCGCCTTCCGTCACACTAGCACCCTGGCTGCTATGAAACTGATGACCTC CCTGGTAAAAGTTGCCCTCCAACTGAGTGTGCACCAAGATAACAATCAGCGTCAGTATGAGGCTGAAAGA AACAAGGGGCCAGGGCAGAGGGCACCTGAGCGGCTGGAGAGCCTGTTGGAGAAACGCAAAGAGCTCCAAG AGCATCAAGAGGAGATTGAGGGGATGATGAATGCCCTCTTCAGGGGTGTCTTTGTTCATCGGTACAGGGA TGTCCTTCCTGAGATCCGTGCTATCTGCATTGAGGAAATTGGGTGTTGGATGCAAAGCTACAGCACGTCT TTCCTCACCGACAGCTATTTAAAATATATTGGTTGGACTCTGCATGATAAGCACCGAGAAGTCCGCCTGA AGTGTGTGAAGGCCCTGAAAGGGCTGTACGGTAACCGGGACCTGACCACACGCCTGGAGCTCTTCACCAG CCGCTTCAAGGACCGGATGGTTTCCATGGTCATGGACAGAGAGTATGATGTGGCAGTGGAGGCTGTCAGA TTACTGATACTTATCCTTAAGAACATGGAAGGGGTGCTGACGGACGCGGATTGTGAGAGCGTCTACCCAG TTGTGTATGCCTCTCATCGAGGCCTGGCCTCTGCCGCAGGCGAATTTCTGTACTGGAAACTCTTCTACCC TGAGTGCGAGATAAGAATGATGGGTGGAAGAGAGCAACGCCAGAGCCCAGGCGCCCAGAGGACTTTCTTC CAGCTTCTGCTGTCCTTCTTTGTGGAGAGCGAGCTCCATGACCACGCTGCTTACTTAGTAGACAGTCTGT GGGACTGTGCAGGGGCTCGGCTGAAGGACTGGGAGGGTCTGACAAGCCTGCTGCTGGAGAAGGACCAGAA CCTGGGTGATGTGCAGGAGAGCACACTGATAGAAATCCTTGTGTCCAGTGCCCGGCAAGCTTCAGAGGGG CACCCGCCTGTGGGCCGGGTCACTGGGAGGAAGGGCTTAACCTCTAAGGAGCGCAAGACCCAAGCCGATG ACAGGGTGAAGTTGACTGAGCACCTCATCCCCCTGCTGCCCCAGCTCCTGGCCAAGTTCTCAGCTGATGC AGAGAAGGTCACTCCCCTGCTCCAGCTTCTCAGCTGCTTTGACCTCCACATCTACTGCACTGGGCGCTTG GAGAAGCACCTGGAGCTGTTCCTGCAGCAACTCCAGGAGGTGGTGGTGAAGCATGCAGAGCCAGCGGTGC TTGAGGCTGGGGCGCATGCCCTCTACCTGCTCTGTAATCCCGAATTCACTTTCTTCAGCCGGGCGGACTT TGCCCGCAGCCAGCTAGTAGATTTGCTGACTGACCGCTTCCAGCAGGAGCTTGAAGAGCTGTTACAGTCG TCCTTCCTAGATGAGGATGAGGTATATAATCTGGCAGCCACTCTGAAACGCCTCTCTGCCTTCTACAACA CTCATGACCTGACTCGCTGGGAGCTCTATGAGCCATGTTGCCAACTCCTGCAGAAGGCTGTGGACACAGG AGAGGTTCCTCACCAGGTTATCCTGCCAGCCTTGACTCTTGTCTATTTTTCCATTCTCTGGACACTAACC CACATTTCTAAATCAGATGCTTCCCAGAAGCAGCTGTCGAGTTTGAGGGACAGAATGGTGGCCTTCTGTG AACTCTGCCAGAGTTGCCTCTCAGATGTGGATACTGAGATCCAGGAGCAGGCTTTTGTCTTATTAAGTGA TCTACTTCTCATCTTTAGCCCTCAGATGATTGTTGGGGGCCGTGATTTCCTTAGGCCACTTGTCTTTTTT CCTGAAGCTACTCTCCAGTCTGAGCTAGCCAGCTTCCTCATGGACCACGTCTTCATCCAGCCGGGAGACC TGGGCAGTGGTGATTCCCAGGAGGATCATTTACAGATAGAGCGGCTACACCAGCGGCGCCGCCTCCTAGC CGGGTTCTGCAAGCTGTTGCTTTATGGGGTGCTGGAGATGGATGCAGCCTCAGATGTTTTCAAACACTAC AACAAGTTCTACAATGACTATGGTGACATTATCAAGGAAACATTAACTAGAGCAAGGCAGATTGACCGAA GTCATTGTTCCCGAATCCTGCTGCTGAGCCTCAAGCAGCTGTACACAGAACTGCTGCAGGAGCATGGGCC CCAGGGCCTGAATGAGCTTCCTGCCTTCATCGAGATGAGGGACCTGGCCCGGAGGTTTGCCTTGAGTTTT GGACCCCAGCAGCTGCAGAACCGTGACCTCGTGGTCATGCTACACAAGGAAGGCATCCAGTTCTCCTTGT CTGAGCTTCCTCCAGCTGGCTCCTCCAATCAGCCTCCAAATCTGGCATTCCTGGAGCTCCTTTCAGAGTT TTCCCCCCGACTCTTCCATCAGGACAAGCAGCTTTTACTGTCCTATCTAGAAAAGTGCCTGCAGCATGTC TCCCAGGCACCTGGCCATCCCTGGGGCCCAGTCACCACCTACTGCCACTCCCTCAGCCCTGTGGAGAACA CAGCAGAGACCAGCCCTCAGGTCCTCCCCAGCTCCAAGAGGAGGCGCGTTGAAGGGCCTGCCAAGCCTAA CAGAGAGGACGTCTCCTCGTCCCAGGAAGAAAGTCTGCAGCTGAACAGCATCCCGCCCACGCCCACCCTC ACCTCCACAGCTGTGAAGAGCAGGCAGCCCCTGTGGGGGTTGAAAGAGATGGAGGAAGAAGATGGCTCAG AGTTGGATTTTGCCCAGGGTCAGCCCGTCGCAGGCACCGAGAGGTCAAGGTTCTTGGGTCCACAATATTT CCAGACTCCACACAACCCTTCAGGTCCTGGCCTGGGCAACCAGCTGATGCGACTCAGCCTTATGGAAGAG GACGAGGAAGAAGAGTTAGAAATCCAGGATGAGTCAAATGAAGAACGGCAGGATACAGACATGCAAGCAA GTAGCTACTCTTCCACCAGTGAGCGCGGGCTGGACCTCTTAGATTCTACAGAGCTGGATATTGAGGATTT C ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240016 representing NM_001282718 Red=Cloning site Green=Tags(s) MSSPLQRAVGDTKRALSASSSSSASLPFDDRDSNHTSEGNGDSLLADEDTDFEDSLNRNVKKRAAKRPPK TTPVAKHPKKGSRVVHRHSRKQSEPPANDLFNAVKAAKSDMQDSGDYPLIAPGPSWKKFQGSFCEFVRTL VCQCQYSLLYDGFPMDDLISLLTGLSDSQVRAFRHTSTLAAMKLMTSLVKVALQLSVHQDNNQRQYEAER NKGPGQRAPERLESLLEKRKELQEHQEEIEGMMNALFRGVFVHRYRDVLPEIRAICIEEIGCWMQSYSTS FLTDSYLKYIGWTLHDKHREVRLKCVKALKGLYGNRDLTTRLELFTSRFKDRMVSMVMDREYDVAVEAVR LLILILKNMEGVLTDADCESVYPVVYASHRGLASAAGEFLYWKLFYPECEIRMMGGREQRQSPGAQRTFF QLLLSFFVESELHDHAAYLVDSLWDCAGARLKDWEGLTSLLLEKDQNLGDVQESTLIEILVSSARQASEG HPPVGRVTGRKGLTSKERKTQADDRVKLTEHLIPLLPQLLAKFSADAEKVTPLLQLLSCFDLHIYCTGRL EKHLELFLQQLQEVVVKHAEPAVLEAGAHALYLLCNPEFTFFSRADFARSQLVDLLTDRFQQELEELLQS SFLDEDEVYNLAATLKRLSAFYNTHDLTRWELYEPCCQLLQKAVDTGEVPHQVILPALTLVYFSILWTLT HISKSDASQKQLSSLRDRMVAFCELCQSCLSDVDTEIQEQAFVLLSDLLLIFSPQMIVGGRDFLRPLVFF PEATLQSELASFLMDHVFIQPGDLGSGDSQEDHLQIERLHQRRRLLAGFCKLLLYGVLEMDAASDVFKHY NKFYNDYGDIIKETLTRARQIDRSHCSRILLLSLKQLYTELLQEHGPQGLNELPAFIEMRDLARRFALSF GPQQLQNRDLVVMLHKEGIQFSLSELPPAGSSNQPPNLAFLELLSEFSPRLFHQDKQLLLSYLEKCLQHV SQAPGHPWGPVTTYCHSLSPVENTAETSPQVLPSSKRRRVEGPAKPNREDVSSSQEESLQLNSIPPTPTL TSTAVKSRQPLWGLKEMEEEDGSELDFAQGQPVAGTERSRFLGPQYFQTPHNPSGPGLGNQLMRLSLMEE DEEEELEIQDESNEERQDTDMQASSYSSTSERGLDLLDSTELDIEDF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282718
ORF Size	3501 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282718.2
RefSeq Size	4187 bp
RefSeq ORF	3504 bp
Locus ID	10734
UniProt ID	Q9UJ98
Protein Pathways	Oocyte meiosis
MW	132.8 kDa
Gene Summary	The protein encoded by this gene is expressed in the nucleus and is a subunit of the cohesin complex which regulates the cohesion of sister chromatids during cell division. A mutation in this gene is associated with premature ovarian failure. Alternate splicing results in multiple transcript variants encoding distinct isoforms. This gene has multiple pseudogenes. [provided by RefSeq, Apr 2014]

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