Aquaporin 3 (AQP3) (NM_004925) Human Tagged ORF Clone
CAT#: RG201856
- TrueORF®
AQP3 (tGFP-tagged) - Human aquaporin 3 (Gill blood group) (AQP3)
ORF Plasmid: DDK
"NM_004925" in other vectors (7)
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CNY 4,000.00
CNY 4,370.00
Cited in 2 publications. |
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AQP-3; GIL |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG201856 representing NM_004925
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTCGACAGAAGGAGCTGGTGTCCCGCTGCGGGGAGATGCTCCACATCCGCTACCGGCTGCTCCGAC AGGCGCTGGCCGAGTGCCTGGGGACCCTCATCCTGGTGATGTTTGGCTGTGGCTCCGTGGCCCAGGTTGT GCTCAGCCGGGGCACCCACGGTGGTTTCCTCACCATCAACCTGGCCTTTGGCTTTGCTGTCACTCTGGGC ATCCTCATCGCTGGCCAGGTCTCTGGGGCCCACCTGAACCCTGCCGTGACCTTTGCCATGTGCTTCCTGG CTCGTGAGCCCTGGATCAAGCTGCCCATCTACACCCTGGCACAGACGCTGGGAGCCTTCTTGGGTGCTGG AATAGTTTTTGGGCTGTATTATGATGCAATCTGGCACTTCGCCGACAACCAGCTTTTTGTTTCGGGCCCC AATGGCACAGCCGGCATCTTTGCTACCTACCCCTCTGGACACTTGGATATGATCAATGGCTTCTTTGACC AGTTCATAGGCACAGCCTCCCTTATCGTGTGTGTGCTGGCCATTGTTGACCCCTACAACAACCCCGTCCC CCGAGGCCTGGAGGCCTTCACCGTGGGCCTGGTGGTCCTGGTCATTGGCACCTCCATGGGCTTCAACTCC GGCTATGCCGTCAACCCTGCCCGGGACTTTGGCCCCCGCCTTTTTACAGCCCTTGCGGGCTGGGGCTCTG CAGTCTTCACGACCGGCCAGCATTGGTGGTGGGTGCCCATCGTGTCCCCACTCCTGGGCTCCATTGCGGG TGTCTTCGTGTACCAGCTGATGATCGGCTGCCACCTGGAGCAGCCCCCACCCTCCAACGAGGAAGAGAAT GTGAAGCTGGCCCATGTGAAGCACAAGGAGCAGATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG201856 representing NM_004925
Red=Cloning site Green=Tags(s) MGRQKELVSRCGEMLHIRYRLLRQALAECLGTLILVMFGCGSVAQVVLSRGTHGGFLTINLAFGFAVTLG ILIAGQVSGAHLNPAVTFAMCFLAREPWIKLPIYTLAQTLGAFLGAGIVFGLYYDAIWHFADNQLFVSGP NGTAGIFATYPSGHLDMINGFFDQFIGTASLIVCVLAIVDPYNNPVPRGLEAFTVGLVVLVIGTSMGFNS GYAVNPARDFGPRLFTALAGWGSAVFTTGQHWWWVPIVSPLLGSIAGVFVYQLMIGCHLEQPPPSNEEEN VKLAHVKHKEQI TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_004925 |
ORF Size | 876 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004925.5 |
RefSeq Size | 1835 bp |
RefSeq ORF | 879 bp |
Locus ID | 360 |
UniProt ID | Q92482 |
Domains | MIP |
Protein Families | Druggable Genome, Transmembrane |
Gene Summary | This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Upregulation of aquaporin-3 is involved in keratinocyte proliferation and epidermal hyperplasia.
,null,
The Journal of investigative dermatology
,PubMed ID 21191421
[AQP3]
|
Aquaporin-3 mediates hydrogen peroxide uptake to regulate downstream intracellular signaling
,null,
Proceedings of the National Academy of Sciences of the United States of America
,PubMed ID 20724658
[AQP3]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201856 | AQP3 (Myc-DDK-tagged)-Human aquaporin 3 (Gill blood group) (AQP3) |
CNY 2,400.00 |
|
RC201856L1 | Lenti ORF clone of Human aquaporin 3 (Gill blood group) (AQP3), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC201856L2 | Lenti ORF clone of Human aquaporin 3 (Gill blood group) (AQP3), mGFP tagged |
CNY 5,890.00 |
|
RC201856L3 | Lenti ORF clone of Human aquaporin 3 (Gill blood group) (AQP3), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC201856L4 | Lenti ORF clone of Human aquaporin 3 (Gill blood group) (AQP3), mGFP tagged |
CNY 5,890.00 |
|
SC117030 | AQP3 (untagged)-Human aquaporin 3 (Gill blood group) (AQP3) |
CNY 2,400.00 |
|
SC322406 | AQP3 (untagged)-Human aquaporin 3 (Gill blood group) (AQP3) |
CNY 2,400.00 |
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