ABHD5 (NM_016006) Human Tagged ORF Clone

CAT#: RG201869

  • TrueORF®

ABHD5 (tGFP-tagged) - Human abhydrolase domain containing 5 (ABHD5)

ORF Plasmid: DDK tGFP



  "NM_016006" in other vectors (7)

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CNY 4,370.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Anti-ABHD5 Rabbit Polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms CGI58; IECN2; NCIE2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG201869 representing NM_016006
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGAGGAGGAGGAGGTGGACTCTGCCGACACCGGAGAGAGGTCAGGATGGCTAACTGGTTGGC
TCCCCACATGGTGCCCTACGTCTATATCACACCTTAAAGAAGCTGAAGAGAAGATGTTAAAATGTGTGCC
TTGCACATACAAAAAAGAACCTGTTCGTATATCTAATGGAAATAAAATATGGACACTGAAGTTCTCTCAT
AATATTTCAAATAAGACTCCACTTGTCCTTCTCCATGGTTTTGGAGGAGGTCTTGGGCTCTGGGCACTGA
ATTTTGGAGATCTTTGCACCAACAGACCTGTCTATGCTTTTGACCTATTGGGTTTTGGACGAAGTAGTAG
ACCCAGGTTTGACAGTGATGCAGAAGAAGTGGAGAATCAGTTTGTGGAATCCATTGAAGAGTGGAGATGT
GCCCTAGGATTGGACAAAATGATCTTGCTTGGGCACAACCTAGGTGGATTCTTGGCTGCTGCTTACTCGC
TGAAGTACCCATCAAGGGTTAATCATCTCATTTTAGTGGAGCCTTGGGGTTTCCCTGAACGACCAGACCT
TGCTGATCAAGACAGACCAATTCCAGTTTGGATCAGAGCCTTGGGAGCAGCATTGACTCCCTTTAACCCT
TTAGCTGGCCTAAGGATTGCAGGACCCTTTGGTTTAAGTCTAGTGCAGCGTTTAAGGCCTGATTTCAAAC
GAAAGTATTCTTCAATGTTCGAAGACGATACTGTGACAGAATACATCTACCACTGTAATGTGCAGACTCC
AAGTGGTGAGACAGCTTTCAAGAATATGACTATTCCTTATGGATGGGCAAAAAGGCCAATGCTCCAGCGA
ATTGGTAAAATGCACCCTGACATTCCAGTTTCAGTGATCTTTGGCGCCCGATCCTGCATAGATGGCAATT
CTGGCACCAGCATCCAGTCCTTACGACCACATTCATATGTGAAGACAATAGCTATTCTTGGGGCAGGACA
TTATGTATATGCAGATCAACCAGAAGAATTCAACCAGAAAGTAAAGGAGATCTGCGACACTGTGGAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG201869 representing NM_016006
Red=Cloning site Green=Tags(s)

MAAEEEEVDSADTGERSGWLTGWLPTWCPTSISHLKEAEEKMLKCVPCTYKKEPVRISNGNKIWTLKFSH
NISNKTPLVLLHGFGGGLGLWALNFGDLCTNRPVYAFDLLGFGRSSRPRFDSDAEEVENQFVESIEEWRC
ALGLDKMILLGHNLGGFLAAAYSLKYPSRVNHLILVEPWGFPERPDLADQDRPIPVWIRALGAALTPFNP
LAGLRIAGPFGLSLVQRLRPDFKRKYSSMFEDDTVTEYIYHCNVQTPSGETAFKNMTIPYGWAKRPMLQR
IGKMHPDIPVSVIFGARSCIDGNSGTSIQSLRPHSYVKTIAILGAGHYVYADQPEEFNQKVKEICDTVD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016006
ORF Size 1047 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_016006.6
RefSeq Size 1536 bp
RefSeq ORF 1050 bp
Locus ID 51099
UniProt ID Q8WTS1
Domains abhydrolase
Protein Families Protease
Gene Summary The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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