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Glypican 4 (GPC4) (NM_001448) Human Tagged ORF Clone
CAT＃: RG202022
- TrueORF®
GPC4 (tGFP-tagged) - Human glypican 4 (GPC4)
ORF Plasmid: DDK
"NM_001448" in other vectors (7)
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CNY 4,370.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | K-glypican; KPTS |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG202022 representing NM_001448
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACGGTTCGGCTTGCCCGCGCTTCTCTGCACCCTGGCAGTGCTCAGCGCCGCGCTGCTGGCTGCCG AGCTCAAGTCGAAAAGTTGCTCGGAAGTGCGACGTCTTTACGTGTCCAAAGGCTTCAACAAGAACGATGC CCCCCTCCACGAGATCAACGGTGATCATTTGAAGATCTGTCCCCAGGGTTCTACCTGCTGCTCTCAAGAG ATGGAGGAGAAGTACAGCCTGCAAAGTAAAGATGATTTCAAAAGTGTGGTCAGCGAACAGTGCAATCATT TGCAAGCTGTCTTTGCTTCACGTTACAAGAAGTTTGATGAATTCTTCAAAGAACTACTTGAAAATGCAGA GAAATCCCTGAATGATATGTTTGTGAAGACATATGGCCATTTATACATGCAAAATTCTGAGCTATTTAAA GATCTCTTCGTAGAGTTGAAACGTTACTACGTGGTGGGAAATGTGAACCTGGAAGAAATGCTAAATGACT TCTGGGCTCGCCTCCTGGAGCGGATGTTCCGCCTGGTGAACTCCCAGTACCACTTTACAGATGAGTATCT GGAATGTGTGAGCAAGTATACGGAGCAGCTGAAGCCCTTCGGAGATGTCCCTCGCAAATTGAAGCTCCAG GTTACTCGTGCTTTTGTAGCAGCCCGTACTTTCGCTCAAGGCTTAGCGGTTGCGGGAGATGTCGTGAGCA AGGTCTCCGTGGTAAACCCCACAGCCCAGTGTACCCATGCCCTGTTGAAGATGATCTACTGCTCCCACTG CCGGGGTCTCGTGACTGTGAAGCCATGTTACAACTACTGCTCAAACATCATGAGAGGCTGTTTGGCCAAC CAAGGGGATCTCGATTTTGAATGGAACAATTTCATAGATGCTATGCTGATGGTGGCAGAGAGGCTAGAGG GTCCTTTCAACATTGAATCGGTCATGGATCCCATCGATGTGAAGATTTCTGATGCTATTATGAACATGCA GGATAATAGTGTTCAAGTGTCTCAGAAGGTTTTCCAGGGATGTGGACCCCCCAAGCCCCTCCCAGCTGGA CGAATTTCTCGTTCCATCTCTGAAAGTGCCTTCAGTGCTCGCTTCAGACCACATCACCCCGAGGAACGCC CAACCACAGCAGCTGGCACTAGTTTGGACCGACTGGTTACTGATGTCAAGGAGAAACTGAAACAGGCCAA GAAATTCTGGTCCTCCCTTCCGAGCAACGTTTGCAACGATGAGAGGATGGCTGCAGGAAACGGCAATGAG GATGACTGTTGGAATGGGAAAGGCAAAAGCAGGTACCTGTTTGCAGTGACAGGAAATGGATTAGCCAACC AGGGCAACAACCCAGAGGTCCAGGTTGACACCAGCAAACCAGACATACTGATCCTTCGTCAAATCATGGC TCTTCGAGTGATGACCAGCAAGATGAAGAATGCATACAATGGGAACGACGTGGACTTCTTTGATATCAGT GATGAAAGTAGTGGAGAAGGAAGTGGAAGTGGCTGTGAGTATCAGCAGTGCCCTTCAGAGTTTGACTACA ATGCCACTGACCATGCTGGGAAGAGTGCCAATGAGAAAGCCGACAGTGCTGGTGTCCGTCCTGGGGCACA GGCCTACCTCCTCACTGTCTTCTGCATCTTGTTCCTGGTTATGCAGAGAGAGTGGAGA AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG202022 representing NM_001448
Red=Cloning site Green=Tags(s) MARFGLPALLCTLAVLSAALLAAELKSKSCSEVRRLYVSKGFNKNDAPLHEINGDHLKICPQGSTCCSQE MEEKYSLQSKDDFKSVVSEQCNHLQAVFASRYKKFDEFFKELLENAEKSLNDMFVKTYGHLYMQNSELFK DLFVELKRYYVVGNVNLEEMLNDFWARLLERMFRLVNSQYHFTDEYLECVSKYTEQLKPFGDVPRKLKLQ VTRAFVAARTFAQGLAVAGDVVSKVSVVNPTAQCTHALLKMIYCSHCRGLVTVKPCYNYCSNIMRGCLAN QGDLDFEWNNFIDAMLMVAERLEGPFNIESVMDPIDVKISDAIMNMQDNSVQVSQKVFQGCGPPKPLPAG RISRSISESAFSARFRPHHPEERPTTAAGTSLDRLVTDVKEKLKQAKKFWSSLPSNVCNDERMAAGNGNE DDCWNGKGKSRYLFAVTGNGLANQGNNPEVQVDTSKPDILILRQIMALRVMTSKMKNAYNGNDVDFFDIS DESSGEGSGSGCEYQQCPSEFDYNATDHAGKSANEKADSAGVRPGAQAYLLTVFCILFLVMQREWR SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001448 |
| ORF Size | 1668 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001448.3 |
| RefSeq Size | 3714 bp |
| RefSeq ORF | 1671 bp |
| Locus ID | 2239 |
| UniProt ID | O75487 |
| Domains | Glypican |
| Protein Families | Druggable Genome |
| Gene Summary | Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3' end of GPC3 and may also play a role in Simpson-Golabi-Behmel syndrome. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Downregulation of glypican-4 facilitates breast cancer progression by inducing cell migration and proliferation
,Munir, J;Van Ngu, T;Na Ayudthaya, PD;Ryu, S;,
Biochem. Biophys. Res. Commun.
,PubMed ID 32199612
[GPC4]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202022 | GPC4 (Myc-DDK-tagged)-Human glypican 4 (GPC4) |
CNY 3,990.00 |
|
| RC202022L1 | Lenti ORF clone of Human glypican 4 (GPC4), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202022L2 | Lenti ORF clone of Human glypican 4 (GPC4), mGFP tagged |
CNY 5,890.00 |
|
| RC202022L3 | Lenti ORF clone of Human glypican 4 (GPC4), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202022L4 | Lenti ORF clone of Human glypican 4 (GPC4), mGFP tagged |
CNY 5,890.00 |
|
| SC110893 | GPC4 (untagged)-Human glypican 4 (GPC4) |
CNY 2,950.00 |
|
| SC322501 | GPC4 (untagged)-Human glypican 4 (GPC4) |
CNY 2,950.00 |
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