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USP33 (NM_201626) Human Tagged ORF Clone
CAT＃: RG203698
- TrueORF®
USP33 (tGFP-tagged) - Human ubiquitin specific peptidase 33 (USP33), transcript variant 3
ORF Plasmid: DDK
"NM_201626" in other vectors (4)
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CNY 9,120.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | VDU1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG203698 representing NM_201626
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGGATCAAATTCACACATAACGATATTAACCTTAAAGGTGTTACCTCATTTTGAAAGTCTTGGGA AACAGGAAAAAATTCCTAACAAAATGTCAGCTTTTCGAAATCATTGTCCACATTTGGATTCAGTTGGTGA AATAACAAAAGAAGATTTGATACAAAAATCCCTTGGTACTTGTCAGGATTGTAAAGTCCAAGGACCAAAT CTTTGGGCATGTCTGGAGAATAGATGTTCATATGTTGGCTGTGGTGAATCACAAGTAGATCACAGCACCA TACATTCTCAGGAGACAAAGCATTATCTAACTGTGAACCTTACCACTCTTCGAGTATGGTGTTATGCTTG CAGCAAAGAAGTATTTTTGGATAGGAAATTAGGAACTCAGCCTTCATTGCCTCATGTAAGACAACCTCAC CAAATACAAGAAAACAGTGTCCAGGATTTTAAAATACCCAGTAATACAACATTAAAAACTCCTCTGGTTG CCGTATTTGATGATCTGGATATAGAAGCGGATGAAGAAGATGAACTTAGGGCCAGAGGTCTTACAGGTTT GAAAAATATTGGAAATACTTGTTACATGAATGCAGCTTTGCAGGCTCTTTCTAATTGCCCACCTTTGACA CAGTTTTTTCTTGATTGTGGAGGACTAGCTCGAACAGATAAGAAACCTGCCATTTGTAAAAGTTATCTCA AACTAATGACAGAGCTGTGGCATAAAAGCAGGCCAGGATCTGTTGTGCCTACTACTCTGTTTCAAGGAAT TAAAACTGTAAATCCAACATTTCGGGGGTATTCTCAGCAGGATGCTCAAGAATTCCTTCGATGTTTAATG GATTTGCTTCATGAAGAATTGAAAGAGCAAGTCATGGAAGTAGAAGAAGATCCGCAAACCATAACCACTG AGGAGACAATGGAAGAAGACAAGAGCCAGTCGGATGTAGATTTTCAGTCTTGTGAATCTTGTAGCAACAG TGATAGAGCAGAAAATGAAAATGGCTCTAGATGCTTTTCTGAAGATAATAATGAAACAACAATGTTAATT CAGGATGATGAAAACAATTCAGAAATGTCAAAGGATTGGCAAAAAGAGAAGATGTGCAATAAGATTAATA AAGTAAATTCTGAAGGCGAATTTGATAAAGATAGAGACTCTATATCTGAAACAGTCGACTTAAACAACCA GGAAACTGTCAAAGTGCAAATACACAGCAGAGCTTCAGAATATATCACTGATGTCCATTCGAATGACCTG TCTACACCACAGATCCTTCCATCAAATGAAGGTGTTAATCCACGTTTATCGGCAAGCCCTCCTAAATCAG GCAATTTGTGGCCAGGATTGGCACCACCACACAAAAAAGCTCAGTCTGCATCTCCAAAGAGAAAAAAACA GCACAAGAAATACAGAAGTGTTATTTCAGACATATTTGATGGAACAATCATTAGTTCAGTGCAGTGTCTG ACTTGTGACAGGGTGTCTGTAACCCTCGAGACCTTTCAAGATCTGTCCTTGCCAATTCCTGGCAAGGAAG ACCTTGCTAAGCTGCATTCATCAAGTCATCCAACTTCTATAGTCAAAGCAGGATCATGTGGCGAAGCATA TGCTCCACAAGGGTGGATAGCTTTTTTCATGGAATATGTGAAGAGCTGGTTTTGGGGTCCAGTAGTAACC TTGCAAGATTGTCTTGCTGCCTTCTTTGCCAGAGATGAACTAAAAGGTGACAATATGTACAGTTGTGAAA AATGCAAAAAGTTGAGAAATGGAGTGAAGTTTTGTAAAGTACAAAACTTTCCTGAGATTTTGTGCATCCA CCTTAAAAGATTCAGACATGAACTAATGTTTTCCACCAAAATCAGTACCCATGTTTCATTTCCGCTAGAA GGCTTGGATCTTCAGCCATTTCTTGCTAAGGATAGTCCAGCTCAAATTGTGACATATGATCTTCTGTCAG TCATTTGCCATCATGGAACTGCAAGTAGTGGACACTATATAGCCTACTGCCGAAACAATCTAAATAATCT CTGGTATGAATTTGATGATCAGAGTGTCACTGAAGTTTCAGAATCTACTGTACAAAATGCAGAAGCTTAC GTTCTTTTCTATAGGAAGAGCAGCGAAGAGGCACAAAAAGAGAGGAGAAGGATATCAAATTTATTGAACA TAATGGAACCAAGCCTCCTTCAGTTTTATATTTCTCGACAGTGGCTTAATAAATTTAAGACCTTTGCCGA ACCTGGCCCTATTTCAAATAATGACTTTCTTTGTATTCATGGAGGTGTTCCTCCAAGAAAAGCTGGTTAT ATTGAAGACCTGGTTTTGATGCTGCCTCAGAACATTTGGGATAACCTATATAGCAGGTATGGTGGAGGAC CAGCTGTCAACCATCTGTACATTTGTCATACTTGCCAAATTGAGGCGGAGAAAATTGAAAAAAGAAGAAA AACTGAATTGGAAATTTTTATTCGGGTAAAAAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203698 representing NM_201626
Red=Cloning site Green=Tags(s) MTGSNSHITILTLKVLPHFESLGKQEKIPNKMSAFRNHCPHLDSVGEITKEDLIQKSLGTCQDCKVQGPN LWACLENRCSYVGCGESQVDHSTIHSQETKHYLTVNLTTLRVWCYACSKEVFLDRKLGTQPSLPHVRQPH QIQENSVQDFKIPSNTTLKTPLVAVFDDLDIEADEEDELRARGLTGLKNIGNTCYMNAALQALSNCPPLT QFFLDCGGLARTDKKPAICKSYLKLMTELWHKSRPGSVVPTTLFQGIKTVNPTFRGYSQQDAQEFLRCLM DLLHEELKEQVMEVEEDPQTITTEETMEEDKSQSDVDFQSCESCSNSDRAENENGSRCFSEDNNETTMLI QDDENNSEMSKDWQKEKMCNKINKVNSEGEFDKDRDSISETVDLNNQETVKVQIHSRASEYITDVHSNDL STPQILPSNEGVNPRLSASPPKSGNLWPGLAPPHKKAQSASPKRKKQHKKYRSVISDIFDGTIISSVQCL TCDRVSVTLETFQDLSLPIPGKEDLAKLHSSSHPTSIVKAGSCGEAYAPQGWIAFFMEYVKSWFWGPVVT LQDCLAAFFARDELKGDNMYSCEKCKKLRNGVKFCKVQNFPEILCIHLKRFRHELMFSTKISTHVSFPLE GLDLQPFLAKDSPAQIVTYDLLSVICHHGTASSGHYIAYCRNNLNNLWYEFDDQSVTEVSESTVQNAEAY VLFYRKSSEEAQKERRRISNLLNIMEPSLLQFYISRQWLNKFKTFAEPGPISNNDFLCIHGGVPPRKAGY IEDLVLMLPQNIWDNLYSRYGGGPAVNHLYICHTCQIEAEKIEKRRKTELEIFIRVKK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_201626 |
| ORF Size | 2484 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_201626.3 |
| RefSeq Size | 2956 bp |
| RefSeq ORF | 2487 bp |
| Locus ID | 23032 |
| UniProt ID | Q8TEY7 |
| Protein Families | Druggable Genome, Protease |
| Gene Summary | This gene encodes a deubiquinating enzyme important in a variety of processes, including Slit-dependent cell migration and beta-2 adrenergic receptor signaling. The protein is negatively regulated through ubiquitination by von Hippel-Lindau tumor protein (VHL). Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Jun 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203698 | USP33 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 33 (USP33), transcript variant 3 |
CNY 8,270.00 |
|
| RC203698L3 | Lenti-ORF clone of USP33 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 33 (USP33), transcript variant 3 |
CNY 10,170.00 |
|
| RC203698L4 | Lenti-ORF clone of USP33 (mGFP-tagged)-Human ubiquitin specific peptidase 33 (USP33), transcript variant 3 |
CNY 10,170.00 |
|
| SC322391 | USP33 (untagged)-Human ubiquitin specific peptidase 33 (USP33), transcript variant 3 |
CNY 6,072.00 |
