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ZNF539 (ZNF254) (NM_203282) Human Tagged ORF Clone
CAT＃: RG207207
- TrueORF®
ZNF254 (tGFP-tagged) - Human zinc finger protein 254 (ZNF254). Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_203282" in other vectors (2)
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CNY 4,370.00
货期*
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BMZF-5; HD-ZNF1; ZNF91L; ZNF539 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG207207 ORF sequence, codon optimized.
Due to the complexity of NM_203282, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGGTCCACCACGATCCCTGGAAATGGGGCTTCTCACGTTCCGGGACGTAGCCATTGAGTTTAGCC TCGAGGAATGGCAGCATCTGGACATAGCCCAGCAGAACCTGTACCGCAACGTGATGCTTGAGAACTACCG AAATTTGGCCTTTCTGGGGATCGCCGTGAGCAAACCTGATCTGATCACGTGTCTTGAGCAGGGAAAAGAA CCGTGGAATATGAAGAGACATGAAATGGTGGACGAGCCACCGGGCATGTGCCCACACTTTGCCCAGGATC TTTGGCCTGAGCAGGGCATGGAAGATAGCTTTCAAAAGGCAATCCTGCGCAGATACGGGAAGTACGGTCA CGAGAATCTGCAGCTGCGCAAGGGATGTAAAAGTGTCGACGAATACAAAGTCAACAAAGAAGGATATAAT GGTCTTAACCAGTGTTTCACAACGGCCCAGAGCAAGGTGTTCCAGTGCGATAAATATCTCAAGGTGTTCT ACAAATTTCTTAATTCCAACAGACCAAAGATCCGGCATACCGAGAAAAAGTCTTTTAAGTGCAAAAAGCG GGTCAAGCTTTTTTGCATGCTGTCTCATAAGACCCAGCACAAAAGTATATACCACCGGGAAAAATCTTAT AAGTGCAAGGAGTGTGGGAAAACTTTTAACTGGAGTTCAACGTTGACGAATCACAGGAAAATCTATACTG AGGAAAAGCCTTACAAATGCGAGGAGTATAACAAGTCTCCAAAGCAGTTGTCAACCCTTACTACACATGA GATTATCCACGCCGGCGAAAAGCTGTACAAATGTGAAGAATGCGGAGAAGCTTTCAATAGGTCATCAAAC CTCACCACACATAAAATTATCCACACCGGCGAAAAGCCTTACAAGTGCGAGGAGTGCGGAAAGGCATTTA TCTGGAGTAGCACGCTTACAGAACATAAAAAGATCCACACTCGAAAGAAACCATATAAGTGCGAAGAGTG CGGTAAGGCATTTATCTGGTCTTCCACACTCACTAGGCATAAAAGAATGCACACCGGGGAAAAACCATAT AAGTGTGAAGAATGCGGAAAGGCTTTCTCACAGTCCAGCACACTGACCACTCACAAGATAATTCACACCG GGGAGAAACGCTACAAATGCCTCGAGTGCGGAAAGGCTTTTAAACAGCTGTCAACCCTCACAACCCATAA GATTATACACGTCGGGGAAAAGCTCTACAAGTGCGAGGAGTGCGGGAAGGGATTCAATCGGAGCAGCAAC CTGACAACCCATAAAATAATTCACACAGGAGAAAAGCCGTACAAGTGCGAGGAGTGTGGTAAAGCATTTA TCTGGAGCAGCACACTGACTAAACACAAGAGAATTCACACGAGGGAAAAGCCCTACAAGTGTGAAGAGTG TGGGAAGGCATTTATCTGGTCTAGCACCCTTACTCGACACAAGAGAATGCATACAGGAGAGAAGCCCTAT AAGTGCGAGGAATGCGGCAAGTCTTTTTCCCAGTCCTCTACTCTCACTACCCATAAGATAATCCACACTG GTGAGAAGCCTTATAAATGCGAAGAATGCGGGAAGGCATTTAACTGGAGCTCTACTCTCACTAAACACAA AATCATCCATACCGAGGAGAAACCATATAAATGCGAAAAATGTGGTAAGGCATTTAAACAGTCCAGCATA CTCACCAATCACAAACGGATTCACACCGGGGAGAAGCCCTACAAATGCGAGGAATGCGGCAAGTCCTTTA ACAGGTCATCTACTTTTACCAAGCACAAAGTTATCCATACTGGCGTAAAACCTTACAAGTGCGAGGAGTG TGGCAAGGCATTCTTTTGGTCTAGCACACTGACAAAGCACAAAAGAATACATACCGGGGAACAACCTTAC AAATGGGAAAAATTTGGCAAGGCGTTCAACCGCAGCAGTCATCTGACTACTGACAAGATCACTCACTGGC GCGAAATTCTTCAAGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG207207 representing NM_203282
Red=Cloning site Green=Tags(s) MPGPPRSLEMGLLTFRDVAIEFSLEEWQHLDIAQQNLYRNVMLENYRNLAFLGIAVSKPDLITCLEQGKE PWNMKRHEMVDEPPGMCPHFAQDLWPEQGMEDSFQKAILRRYGKYGHENLQLRKGCKSVDEYKVNKEGYN GLNQCFTTAQSKVFQCDKYLKVFYKFLNSNRPKIRHTEKKSFKCKKRVKLFCMLSHKTQHKSIYHREKSY KCKECGKTFNWSSTLTNHRKIYTEEKPYKCEEYNKSPKQLSTLTTHEIIHAGEKLYKCEECGEAFNRSSN LTTHKIIHTGEKPYKCEECGKAFIWSSTLTEHKKIHTRKKPYKCEECGKAFIWSSTLTRHKRMHTGEKPY KCEECGKAFSQSSTLTTHKIIHTGEKRYKCLECGKAFKQLSTLTTHKIIHVGEKLYKCEECGKGFNRSSN LTTHKIIHTGEKPYKCEECGKAFIWSSTLTKHKRIHTREKPYKCEECGKAFIWSSTLTRHKRMHTGEKPY KCEECGKSFSQSSTLTTHKIIHTGEKPYKCEECGKAFNWSSTLTKHKIIHTEEKPYKCEKCGKAFKQSSI LTNHKRIHTGEKPYKCEECGKSFNRSSTFTKHKVIHTGVKPYKCEECGKAFFWSSTLTKHKRIHTGEQPY KWEKFGKAFNRSSHLTTDKITHWREILQV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_203282 |
| ORF Size | 1977 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_203282.2, NP_975011.3 |
| RefSeq Size | 4127 bp |
| RefSeq ORF | 1980 bp |
| Locus ID | 9534 |
| UniProt ID | O75437 |
| Protein Families | Transcription Factors |
| Gene Summary | Zinc finger proteins have been shown to interact with nucleic acids and to have diverse functions. The zinc finger domain is a conserved amino acid sequence motif containing 2 specifically positioned cysteines and 2 histidines that are involved in coordinating zinc. Kruppel-related proteins form 1 family of zinc finger proteins. See ZFP93 (MIM 604749) for additional information on zinc finger proteins.[supplied by OMIM, Jul 2002] |
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