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EXOC3 (NM_007277) Human Tagged ORF Clone
CAT＃: RG209413
- TrueORF®
EXOC3 (tGFP-tagged) - Human exocyst complex component 3 (EXOC3)
ORF Plasmid: DDK
"NM_007277" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | SEC6; SEC6L1; Sec6p |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG209413 representing NM_007277
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGAGACAGACCGGGAGGCCGTTGCGACAGCAGTGCAAAGGGTTGCTGGGATGCTCCAGCGCCCGG ACCAGCTGGACAAGGTGGAGCAGTATCGCAGGAGAGAAGCGCGGAAGAAGGCCTCCGTGGAGGCCAGATT GAAGGCCGCCATCCAGTCACAGTTGGACGGGGTGCGCACAGGCCTCAGCCAGCTCCACAACGCCCTGAAT GACGTCAAAGACATCCAGCAGTCGCTGGCAGACGTCAGCAAGGACTGGAGGCAGAGCATCAACACCATTG AGAGCCTCAAGGACGTCAAAGACGCCGTGGTGCAGCACAGCCAGCTCGCCGCAGCCGTGGAGAACCTCAA GAACATCTTCTCAGTGCCTGAGATTGTGAGGGAGACCCAGGACCTAATTGAACAAGGGGCACTCCTGCAA GCCCACCGGAAGCTGATGGACCTGGAATGCTCCCGGGACGGGCTGATGTACGAGCAGTACCGCATGGACA GTGGGAACACGCGTGACATGACCCTCATCCATGGCTACTTTGGCAGCACGCAGGGGCTCTCTGATGAGCT GGCTAAGCAGCTGTGGATGGTGCTGCAGAGGTCACTGGTCACTGTCCGCCGTGACCCCACCTTGCTGGTC TCAGTTGTCAGGATCATTGAAAGGGAAGAGAAAATTGACAGGCGCATACTTGACCGGAAAAAGCAAACTG GCTTTGTTCCTCCTGGGAGGCCCAAGAATTGGAAGGAGAAAATGTTCACCATCTTGGAGAGGACTGTGAC CACCAGAATTGAGGGCACACAGGCAGATACCAGAGAGTCTGACAAGATGTGGCTTGTCCGCCACCTGGAA ATTATAAGGAAGTACGTCCTGGATGACCTCATTGTCGCCAAAAACCTGATGGTTCAGTGCTTTCCTCCCC ACTATGAGATCTTTAAGAACCTCCTGAACATGTACCACCAAGCCCTGAGCACGCGGATGCAGGACCTCGC ATCGGAAGACCTGGAAGCCAATGAGATCGTGAGCCTCTTGACGTGGGTCTTAAACACCTACACAAGTACT GAGATGATGAGGAACGTGGAGCTGGCCCCGGAAGTGGATGTCGGCACCCTGGAGCCATTGCTTTCTCCAC ACGTGGTCTCTGAGCTGCTTGACACGTACATGTCCACGCTCACTTCAAACATCATCGCCTGGCTGCGGAA AGCGCTGGAGACAGACAAGAAAGACTGGGTCAAAGAGACAGAGCCAGAAGCCGACCAGGACGGGTACTAC CAGACCACACTCCCTGCCATTGTCTTCCAGATGTTTGAACAGAATCTTCAAGTTGCTGCTCAGATAAGTG AAGATTTGAAAACAAAGGTACTAGTTTTATGTCTTCAGCAGATGAATTCTTTCCTAAGCAGATATAAAGA TGAAGCGCAGCTGTATAAAGAAGAGCACCTGAGGAATCGGCAGCACCCTCACTGCTACGTTCAGTACATG ATCGCCATCATCAACAACTGCCAGACCTTCAAGGAATCCATAGTCAGTTTAAAAAGAAAGTATTTAAAGA ATGAAGTGGAAGAGGGTGTGTCTCCGAGCCAGCCCAGCATGGACGGGATTTTAGACGCCATCGCGAAGGA GGGCTGCAGCGGTTTGCTGGAGGAGGTCTTCCTGGACCTGGAGCAACATCTGAATGAATTGATGACGAAG AAGTGGCTATTAGGGTCAAACGCTGTAGACATTATCTGTGTCACCGTGGAAGACTATTTCAACGATTTTG CCAAAATTAAAAAGCCGTATAAGAAGAGGATGACGGCCGAGGCGCACCGGCGCGTGGTGGTGGAGTACCT GCGGGCGGTCATGCAGAAGCGCATTTCCTTCCGGAGCCCGGAGGAGCGCAAGGAGGGTGCCGAGAAGATG GTTAGGGAGGCAGAGCAGCTGCGCTTCCTGTTCCGGAAGCTGGCGTCCGGTTTCGGGGAAGACGTGGACG GATACTGCGACACCATCGTGGCTGTGGCCGAAGTGATCAAGCTGACAGACCCTTCTCTGCTCTACCTGGA GGTCTCCACTCTGGTCAGCAAGTATCCAGACATCAGGGATGACCACATCGGTGCGCTGCTGGCTGTGCGT GGGGACGCCAGCCGTGACATGAAGCAGACCATCATGGAGACCCTGGAGCAGGGCCCAGCACAGGCCAGCC CCAGCTACGTGCCCCTCTTCAAGGACATTGTGGTGCCCAGCCTGAACGTGGCCAAGCTGCTCAAG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG209413 representing NM_007277
Red=Cloning site Green=Tags(s) MKETDREAVATAVQRVAGMLQRPDQLDKVEQYRRREARKKASVEARLKAAIQSQLDGVRTGLSQLHNALN DVKDIQQSLADVSKDWRQSINTIESLKDVKDAVVQHSQLAAAVENLKNIFSVPEIVRETQDLIEQGALLQ AHRKLMDLECSRDGLMYEQYRMDSGNTRDMTLIHGYFGSTQGLSDELAKQLWMVLQRSLVTVRRDPTLLV SVVRIIEREEKIDRRILDRKKQTGFVPPGRPKNWKEKMFTILERTVTTRIEGTQADTRESDKMWLVRHLE IIRKYVLDDLIVAKNLMVQCFPPHYEIFKNLLNMYHQALSTRMQDLASEDLEANEIVSLLTWVLNTYTST EMMRNVELAPEVDVGTLEPLLSPHVVSELLDTYMSTLTSNIIAWLRKALETDKKDWVKETEPEADQDGYY QTTLPAIVFQMFEQNLQVAAQISEDLKTKVLVLCLQQMNSFLSRYKDEAQLYKEEHLRNRQHPHCYVQYM IAIINNCQTFKESIVSLKRKYLKNEVEEGVSPSQPSMDGILDAIAKEGCSGLLEEVFLDLEQHLNELMTK KWLLGSNAVDIICVTVEDYFNDFAKIKKPYKKRMTAEAHRRVVVEYLRAVMQKRISFRSPEERKEGAEKM VREAEQLRFLFRKLASGFGEDVDGYCDTIVAVAEVIKLTDPSLLYLEVSTLVSKYPDIRDDHIGALLAVR GDASRDMKQTIMETLEQGPAQASPSYVPLFKDIVVPSLNVAKLLK SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007277 |
| ORF Size | 2235 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007277.4, NP_009208.2 |
| RefSeq Size | 2774 bp |
| RefSeq ORF | 2238 bp |
| Locus ID | 11336 |
| UniProt ID | O60645 |
| Protein Pathways | Tight junction |
| Gene Summary | The protein encoded by this gene is a component of the exocyst complex, a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. The complex is also essential for the biogenesis of epithelial cell surface polarity. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209413 | EXOC3 (Myc-DDK-tagged)-Human exocyst complex component 3 (EXOC3) |
CNY 3,990.00 |
|
| RC209413L3 | Lenti ORF clone of Human exocyst complex component 3 (EXOC3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209413L4 | Lenti ORF clone of Human exocyst complex component 3 (EXOC3), mGFP tagged |
CNY 5,890.00 |
|
| SC322701 | EXOC3 (untagged)-Human exocyst complex component 3 (EXOC3) |
CNY 5,464.00 |
