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Hormone sensitive lipase (LIPE) (NM_005357) Human Tagged ORF Clone
CAT＃: RG209729
- TrueORF®
LIPE (tGFP-tagged) - Human lipase, hormone-sensitive (LIPE)
ORF Plasmid: DDK
"NM_005357" in other vectors (6)
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CNY 9,096.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AOMS4; FPLD6; HSL; LHS; REH |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG209729 representing NM_005357
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCAGGTTCTAAGTCAGTGTCTAGGTCAGACTGGCAACCTGAACCACACCAGAGGCCTATAACCC CGCTAGAGCCTGGGCCAGAAAAGACACCCATAGCCCAGCCAGAATCGAAGACTCTGCAGGGATCCAATAC CCAACAGAAGCCTGCTTCAAACCAAAGACCCCTCACCCAGCAGGAGACCCCTGCACAACATGATGCTGAA TCCCAGAAGGAACCTAGAGCCCAACAAAAATCTGCTTCACAAGAGGAATTTCTTGCCCCACAGAAGCCCG CACCACAGCAATCACCTTACATCCAAAGGGTGCTGCTCACTCAACAGGAAGCTGCCTCCCAGCAGGGACC TGGGCTAGGAAAAGAATCTATAACTCAACAGGAGCCAGCATTGAGACAAAGACATGTAGCCCAGCCAGGG CCTGGGCCAGGAGAGCCACCTCCAGCTCAACAAGAAGCTGAATCAACACCTGCGGCCCAGGCTAAACCTG GAGCCAAAAGGGAGCCATCTGCCCCGACTGAATCTACGTCCCAAGAGACACCTGAACAGTCAGACAAGCA AACAACGCCAGTCCAGGGAGCCAAATCCAAGCAGGGATCTTTGACAGAGCTGGGATTTCTAACAAAACTT CAGGAACTATCCATACAGCGATCAGCCCTAGAGTGGAAGGCACTTTCTGAGTGGGTCACAGATTCTGAGT CAGAATCAGATGTGGGATCATCTTCAGACACAGATTCTCCAGCCACGATGGGTGGAATGGTGGCCCAGGG AGTGAAGCTAGGCTTCAAAGGAAAATCTGGTTATAAAGTGATGTCAGGATACAGTGGGACGTCGCCACAT GAGAAAACCAGTGCTCGGAATCACAGACACTACCAGGATACAGCCTCAAGGCTCATCCACAACATGGACC TGCGCACAATGACACAGTCGCTGGTGACTCTGGCGGAGGACAACATAGCCTTCTTCTCGAGCCAGGGTCC TGGGGAAACGGCCCAGCGGCTGTCAGGCGTTTTTGCCGGTGTACGGGAGCAGGCGCTGGGGCTGGAGCCG GCCCTGGGCCGCCTGCTGGGTGTGGCGCACCTCTTTGACCTGGACCCAGAGACACCGGCCAACGGGTACC GCAGCCTAGTGCACACAGCCCGCTGCTGCCTGGCGCACCTCCTGCACAAATCCCGCTATGTGGCCTCCAA CCGCCGCAGCATCTTCTTCCGCACCAGCCACAACCTGGCCGAGCTGGAGGCCTACCTGGCTGCCCTCACC CAGCTCCGCGCTCTGGTCTACTACGCCCAGCGCCTGCTGGTTACCAATCGGCCGGGGGTACTCTTCTTTG AGGGCGACGAGGGGCTCACCGCCGACTTCCTCCGGGAGTATGTCACGCTGCATAAGGGATGCTTCTATGG CCGCTGCCTGGGCTTCCAGTTCACGCCTGCCATCCGGCCATTCCTGCAGACCATCTACATTGGGCTGGTG TCCTTCGGGGAGCACTACAAACGCAACGAGACAGGCCTCAGTGTGGCCGCCAGCTCTCTCTTCACCAGCG GCCGCTTTGCCATCGACCCCGAGCTGCGTGGGGCTGAGTTTGAGCGGATCACACAGAACCTGGACGTGCA CTTCTGGAAAGCCTTCTGGAACATCACCGAGATGGAAGTGCTATCGTCTCTGGCCAACATGGCATCGGCC ACCGTGAGGGTAAGCCGCCTGCTCAGCCTGCCACCCGAAGCCTTTGAGATGCCACTGACTGCCGACCCCA CGCTCACGGTCACCATCTCACCCCCACTGGCCCACACAGGCCCTGGGCCCGTCCTCGTCAGGCTCATCTC CTGTGACCTGCGTGAAGGACAGGACAGTGAGGAGCTCAGCAGCCTGATAAAGTCCAACGGCCAACGGAGC CTGGAGCTGTGGCCGCGCCCCCAGCAGGCACCCCGCTCGCGGTCCCTGATAGTGCACTTCCACGGCGGTG GCTTTGTGGCCCAGACCTCCAGATCCCACGAGCCCTACCTCAAGAGCTGGGCCCAGGAGCTGGGCGCCCC CATCATCTCCATCGACTACTCCCTGGCCCCTGAGGCCCCCTTCCCCCGTGCGCTGGAGGAGTGCTTCTTC GCCTACTGCTGGGCCATCAAGCACTGCGCCCTCCTTGGCTCAACAGGGGAACGAATCTGCCTTGCGGGGG ACAGTGCAGGCGGGAACCTCTGCTTCACCGTGGCTCTTCGGGCAGCAGCCTACGGGGTGCGGGTGCCAGA TGGCATCATGGCAGCCTACCCGGCCACAATGCTGCAGCCTGCCGCCTCTCCCTCCCGCCTGCTGAGCCTC ATGGACCCCTTGCTGCCCCTCAGTGTGCTCTCCAAGTGTGTCAGCGCCTATGCTGGTGCAAAGACGGAGG ACCACTCCAACTCAGACCAGAAAGCCCTCGGCATGATGGGGCTGGTGCGGCGGGACACAGCCCTGCTCCT CCGAGACTTCCGCCTGGGTGCCTCCTCATGGCTCAACTCCTTCCTGGAGTTAAGTGGGCGCAAGTCCCAG AAGATGTCGGAGCCCATAGCAGAGCCGATGCGCCGCAGTGTGTCTGAAGCAGCACTGGCCCAGCCCCAGG GCCCACTGGGCACGGATTCCCTCAAGAACCTGACCCTGAGGGACTTGAGCCTGAGGGGAAACTCCGAGAC GTCGTCGGACACCCCCGAGATGTCGCTGTCAGCTGAGACACTTAGCCCCTCCACACCCTCCGATGTCAAC TTCTTATTACCACCTGAGGATGCAGGGGAAGAGGCTGAGGCCAAAAATGAGCTGAGCCCCATGGACAGAG GCCTGGGCGTCCGTGCCGCCTTCCCCGAGGGTTTCCACCCCCGACGCTCCAGCCAGGGTGCCACACAGAT GCCCCTCTACTCCTCACCCATAGTCAAGAACCCCTTCATGTCGCCGCTGCTGGCACCCGACAGCATGCTC AAGAGCCTGCCACCTGTGCACATCGTGGCGTGCGCGCTGGACCCCATGCTGGACGACTCGGTCATGCTCG CGCGGCGACTGCGCAACCTGGGCCAGCCGGTGACGCTGCGCGTGGTGGAGGACCTGCCGCACGGCTTCCT GACCCTAGCGGCGCTGTGCCGCGAGACGCGCCAGGCCGCAGAGCTGTGCGTGGAGCGCATCCGCCTCGTC CTCACTCCTCCCGCCGGAGCCGGGCCGAGCGGGGAGACGGGGGCTGCGGGGGTAGACGGGGGCTGCGGGG GGCGACAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG209729 representing NM_005357
Red=Cloning site Green=Tags(s) MEPGSKSVSRSDWQPEPHQRPITPLEPGPEKTPIAQPESKTLQGSNTQQKPASNQRPLTQQETPAQHDAE SQKEPRAQQKSASQEEFLAPQKPAPQQSPYIQRVLLTQQEAASQQGPGLGKESITQQEPALRQRHVAQPG PGPGEPPPAQQEAESTPAAQAKPGAKREPSAPTESTSQETPEQSDKQTTPVQGAKSKQGSLTELGFLTKL QELSIQRSALEWKALSEWVTDSESESDVGSSSDTDSPATMGGMVAQGVKLGFKGKSGYKVMSGYSGTSPH EKTSARNHRHYQDTASRLIHNMDLRTMTQSLVTLAEDNIAFFSSQGPGETAQRLSGVFAGVREQALGLEP ALGRLLGVAHLFDLDPETPANGYRSLVHTARCCLAHLLHKSRYVASNRRSIFFRTSHNLAELEAYLAALT QLRALVYYAQRLLVTNRPGVLFFEGDEGLTADFLREYVTLHKGCFYGRCLGFQFTPAIRPFLQTIYIGLV SFGEHYKRNETGLSVAASSLFTSGRFAIDPELRGAEFERITQNLDVHFWKAFWNITEMEVLSSLANMASA TVRVSRLLSLPPEAFEMPLTADPTLTVTISPPLAHTGPGPVLVRLISCDLREGQDSEELSSLIKSNGQRS LELWPRPQQAPRSRSLIVHFHGGGFVAQTSRSHEPYLKSWAQELGAPIISIDYSLAPEAPFPRALEECFF AYCWAIKHCALLGSTGERICLAGDSAGGNLCFTVALRAAAYGVRVPDGIMAAYPATMLQPAASPSRLLSL MDPLLPLSVLSKCVSAYAGAKTEDHSNSDQKALGMMGLVRRDTALLLRDFRLGASSWLNSFLELSGRKSQ KMSEPIAEPMRRSVSEAALAQPQGPLGTDSLKNLTLRDLSLRGNSETSSDTPEMSLSAETLSPSTPSDVN FLLPPEDAGEEAEAKNELSPMDRGLGVRAAFPEGFHPRRSSQGATQMPLYSSPIVKNPFMSPLLAPDSML KSLPPVHIVACALDPMLDDSVMLARRLRNLGQPVTLRVVEDLPHGFLTLAALCRETRQAAELCVERIRLV LTPPAGAGPSGETGAAGVDGGCGGRH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005357 |
| ORF Size | 3228 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005357.2, NP_005348.2 |
| RefSeq Size | 3806 bp |
| RefSeq ORF | 3231 bp |
| Locus ID | 3991 |
| UniProt ID | Q05469 |
| Protein Pathways | Insulin signaling pathway |
| Gene Summary | The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209729 | LIPE (Myc-DDK-tagged)-Human lipase, hormone-sensitive (LIPE) |
CNY 7,496.00 |
|
| RC209729L1 | Lenti ORF clone of Human lipase, hormone-sensitive (LIPE), Myc-DDK-tagged |
CNY 9,896.00 |
|
| RC209729L2 | Lenti ORF clone of Human lipase, hormone-sensitive (LIPE), mGFP tagged |
CNY 7,600.00 |
|
| RC209729L3 | Lenti ORF clone of Human lipase, hormone-sensitive (LIPE), Myc-DDK-tagged |
CNY 9,896.00 |
|
| RC209729L4 | Lenti ORF clone of Human lipase, hormone-sensitive (LIPE), mGFP tagged |
CNY 9,896.00 |
|
| SC303624 | LIPE (untagged)-Human lipase, hormone-sensitive (LIPE) |
CNY 7,496.00 |
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