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Ankyrin G (ANK3) (NM_001149) Human Tagged ORF Clone
CAT＃: RG212082
- TrueORF®
ANK3 (tGFP-tagged) - Human ankyrin 3, node of Ranvier (ankyrin G) (ANK3), transcript variant 2
ORF Plasmid: DDK
"NM_001149" in other vectors (6)
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CNY 11,020.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ANKYRIN-G; MRT37 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG212082 representing NM_001149
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTTACCGCAGAGTGAAGATGCAATGACCGGGGACACAGACAAATATCTTGGGCCACAGGACCTTA AGGAATTGGGTGATGATTCCCTGCCTGCAGAGGGTTACATGGGCTTTAGTCTCGGAGCGCGTTCTGCCAG CCTCCGCTCCTTCAGTTCGGATAGGTCTTACACCTTGAACAGAAGCTCCTATGCACGGGACAGCATGATG ATTGAAGAACTCCTTGTGCCATCCAAAGAGCAGCATCTAACATTCACAAGGGAATTTGATTCAGATTCTC TTAGACATTACAGCTGGGCTGCAGACACCTTAGACAATGTCAATCTTGTTTCAAGCCCCATTCATTCTGG GTTTCTGGTTAGCTTTATGGTGGACGCGAGAGGGGGCTCCATGAGAGGAAGCCGTCATCACGGGATGAGA ATCATCATTCCTCCACGCAAGTGTACGGCCCCCACTCGAATCACCTGCCGTTTGGTAAAGAGACATAAAC TGGCCAACCCACCCCCCATGGTGGAAGGAGAGGGATTAGCCAGTAGGCTGGTAGAAATGGGTCCTGCAGG GGCACAATTTTTAGGCCCTGTCATAGTGGAAATCCCTCACTTTGGGTCCATGAGAGGAAAAGAGAGAGAA CTCATTGTTCTTCGAAGTGAAAATGGTGAAACTTGGAAGGAGCATCAGTTTGACAGCAAAAATGAAGATT TAACCGAGTTACTTAATGGCATGGATGAAGAACTTGATAGCCCAGAAGAGTTAGGGAAAAAGCGTATCTG CAGGATTATCACGAAAGATTTCCCCCAGTATTTTGCAGTGGTTTCCCGGATTAAGCAGGAAAGCAACCAG ATTGGTCCTGAAGGTGGAATTCTGAGCAGCACCACAGTGCCCCTTGTTCAAGCATCTTTCCCAGAGGGTG CCCTAACTAAAAGAATTCGAGTGGGCCTCCAGGCCCAGCCTGTTCCAGATGAAATTGTGAAAAAGATCCT TGGAAACAAAGCAACTTTTAGCCCAATTGTCACTGTGGAACCAAGAAGACGGAAATTCCATAAACCAATC ACAATGACCATTCCGGTGCCCCCGCCCTCAGGAGAAGGTGTATCCAATGGATACAAAGGGGACACTACAC CCAATCTGCGTCTTCTCTGTAGCATTACAGGGGGCACTTCGCCTGCTCAGTGGGAAGACATCACAGGAAC AACTCCTTTGACGTTTATAAAAGATTGTGTCTCCTTTACAACCAATGTTTCAGCCAGATTTTGGCTTGCA GACTGCCATCAAGTTTTAGAAACTGTGGGGTTAGCCACGCAACTGTACAGAGAATTGATATGTGTTCCAT ATATGGCCAAGTTTGTTGTTTTTGCCAAAATGAATGATCCCGTAGAATCTTCCTTGCGATGTTTCTGCAT GACAGATGACAAAGTGGACAAAACTTTAGAGCAACAAGAGAATTTTGAGGAAGTCGCAAGAAGCAAAGAT ATTGAGGTTCTGGAAGGAAAACCTATTTATGTTGATTGTTATGGAAATTTGGCCCCACTTACCAAAGGAG GACAGCAACTTGTTTTTAACTTTTATTCTTTCAAAGAAAATAGACTGCCATTTTCCATCAAGATTAGAGA CACCAGCCAAGAGCCCTGTGGTCGTCTGTCTTTTCTGAAAGAACCAAAGACAACAAAAGGACTGCCTCAA ACAGCGGTTTGCAACTTAAATATCACTCTGCCAGCACATAAAAAGATTGAGAAAACAGATAGACGACAGA GCTTCGCATCCTTAGCTTTACGTAAGCGCTACAGCTACTTGACTGAGCCTGGAATGAGTCCACAGAGTCC ATGTGAACGGACAGATATCAGGATGGCAATAGTAGCCGATCACCTGGGACTTAGTTGGACAGAACTGGCA AGGGAACTGAATTTTTCAGTGGATGAAATCAATCAAATACGTGTGGAAAATCCAAATTCTTTAATTTCTC AGAGCTTCATGTTATTAAAAAAATGGGTTACCAGAGACGGAAAAAATGCCACAACTGATGCCTTAACTTC GGTCTTGACAAAAATTAATCGAATAGATATAGTGACACTGCTAGAAGGACCAATATTTGATTATGGAAAT ATTTCAGGCACCAGAAGTTTTGCAGATGAGAACAATGTTTTCCATGACCCTGTTGATGGTTATCCTTCCC TTCAAGTGGAACTGGAAACCCCCACAGGGTTGCACTACACACCACCTACCCCTTTCCAGCAAGATGATTA TTTTAGTGATATCTCTAGCATAGAATCTCCCCTTAGAACCCCTAGTAGACTGAGTGATGGGCTAGTGCCT TCCCAGGGGAACATAGAGCATTCCGCAGATGGACCTCCAGTCGTAACTGCAGAAGACGCTTCCTTAGAAG ACAGCAAACTGGAAGACTCAGTGCCTTTAACAGAAATGCCTGAAGCAGTGGATGTAGATGAGAGCCAGTT GGAGAATGTATGTCTGAGTTGGCAGAATGAGACATCAAGTGGAAACCTAGAGTCCTGCGCTCAAGCTCGA AGAGTAACTGGTGGGTTACTAGATCGACTGGATGACAGCCCTGACCAGTGTAGAGATTCCATTACCTCAT ATCTCAAAGGAGAAGCTGGCAAATTTGAAGCAAATGGAAGCCATACAGAAATCACTCCAGAAGCAAAGAC AAAATCTTACTTTCCAGAATCCCAAAATGATGTAGGAAAACAGAGTACCAAGGAAACTCTGAAACCAAAA ATACATGGATCTGGTCATGTTGAAGAACCAGCATCACCACTAGCAGCATATCAGAAATCTCTAGAAGAAA CCAGCAAGCTTATAATAGAAGAGACTAAACCCTGTGTGCCTGTCAGTATGAAAAAGATGAGTAGGACTTC TCCAGCAGATGGCAAGCCAAGGCTTAGCCTCCATGAAGAAGAGGGGTCCAGTGGGTCTGAGCAAAAGCAG GGAGAAGGTTTTAAGGTGAAAACGAAGAAAGAAATCCGGCATGTGGAAAAGAAGAGCCACTCG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG212082 representing NM_001149
Red=Cloning site Green=Tags(s) MALPQSEDAMTGDTDKYLGPQDLKELGDDSLPAEGYMGFSLGARSASLRSFSSDRSYTLNRSSYARDSMM IEELLVPSKEQHLTFTREFDSDSLRHYSWAADTLDNVNLVSSPIHSGFLVSFMVDARGGSMRGSRHHGMR IIIPPRKCTAPTRITCRLVKRHKLANPPPMVEGEGLASRLVEMGPAGAQFLGPVIVEIPHFGSMRGKERE LIVLRSENGETWKEHQFDSKNEDLTELLNGMDEELDSPEELGKKRICRIITKDFPQYFAVVSRIKQESNQ IGPEGGILSSTTVPLVQASFPEGALTKRIRVGLQAQPVPDEIVKKILGNKATFSPIVTVEPRRRKFHKPI TMTIPVPPPSGEGVSNGYKGDTTPNLRLLCSITGGTSPAQWEDITGTTPLTFIKDCVSFTTNVSARFWLA DCHQVLETVGLATQLYRELICVPYMAKFVVFAKMNDPVESSLRCFCMTDDKVDKTLEQQENFEEVARSKD IEVLEGKPIYVDCYGNLAPLTKGGQQLVFNFYSFKENRLPFSIKIRDTSQEPCGRLSFLKEPKTTKGLPQ TAVCNLNITLPAHKKIEKTDRRQSFASLALRKRYSYLTEPGMSPQSPCERTDIRMAIVADHLGLSWTELA RELNFSVDEINQIRVENPNSLISQSFMLLKKWVTRDGKNATTDALTSVLTKINRIDIVTLLEGPIFDYGN ISGTRSFADENNVFHDPVDGYPSLQVELETPTGLHYTPPTPFQQDDYFSDISSIESPLRTPSRLSDGLVP SQGNIEHSADGPPVVTAEDASLEDSKLEDSVPLTEMPEAVDVDESQLENVCLSWQNETSSGNLESCAQAR RVTGGLLDRLDDSPDQCRDSITSYLKGEAGKFEANGSHTEITPEAKTKSYFPESQNDVGKQSTKETLKPK IHGSGHVEEPASPLAAYQKSLEETSKLIIEETKPCVPVSMKKMSRTSPADGKPRLSLHEEEGSSGSEQKQ GEGFKVKTKKEIRHVEKKSHS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001149 |
| ORF Size | 3003 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001149.3, NP_001140.2 |
| RefSeq Size | 7218 bp |
| RefSeq ORF | 3006 bp |
| Locus ID | 288 |
| UniProt ID | Q12955 |
| Domains | ZU5, ANK |
| Protein Families | Druggable Genome |
| Gene Summary | Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact, and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 3 is an immunologically distinct gene product from ankyrins 1 and 2, and was originally found at the axonal initial segment and nodes of Ranvier of neurons in the central and peripheral nervous systems. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212082 | ANK3 (Myc-DDK-tagged)-Human ankyrin 3, node of Ranvier (ankyrin G) (ANK3), transcript variant 2 |
CNY 6,968.00 |
|
| RC212082L1 | Lenti-ORF clone of ANK3 (Myc-DDK-tagged)-Human ankyrin 3, node of Ranvier (ankyrin G) (ANK3), transcript variant 2 |
CNY 11,880.00 |
|
| RC212082L2 | Lenti-ORF clone of ANK3 (mGFP-tagged)-Human ankyrin 3, node of Ranvier (ankyrin G) (ANK3), transcript variant 2 |
CNY 9,368.00 |
|
| RC212082L3 | Lenti-ORF clone of ANK3 (Myc-DDK-tagged)-Human ankyrin 3, node of Ranvier (ankyrin G) (ANK3), transcript variant 2 |
CNY 11,880.00 |
|
| RC212082L4 | Lenti-ORF clone of ANK3 (mGFP-tagged)-Human ankyrin 3, node of Ranvier (ankyrin G) (ANK3), transcript variant 2 |
CNY 11,880.00 |
|
| SC310013 | ANK3 (untagged)-Human ankyrin 3, node of Ranvier (ankyrin G) (ANK3), transcript variant 2 |
CNY 19,190.00 |
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