United States
Germany
Japan
United Kingdom
China
GJC2 (NM_020435) Human Tagged ORF Clone
CAT＃: RG212096
- TrueORF®
GJC2 (tGFP-tagged) - Human gap junction protein, gamma 2, 47kDa (GJC2)
ORF Plasmid: DDK
"NM_020435" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 7,088.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CX46.6; Cx47; GJA12; HLD2; LMPH1C; LMPHM3; PMLDAR; SPG44 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG212096 representing NM_020435
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCAACATGAGCTGGAGCTTCCTGACGCGGCTGCTGGAGGAGATCCACAACCACTCCACCTTCGTGG GCAAGGTGTGGCTCACGGTGCTGGTGGTCTTCCGCATCGTGCTGACGGCTGTGGGCGGCGAGGCCATCTA CTCGGACGAGCAGGCCAAGTTCACTTGCAACACGCGGCAGCCAGGCTGCGACAACGTCTGCTATGACGCC TTCGCGCCCCTGTCGCACGTGCGCTTCTGGGTCTTCCAGATTGTGGTCATCTCCACGCCCTCGGTCATGT ACCTGGGCTACGCCGTGCACCGCCTGGCCCGTGCGTCTGAGCAGGAGCGGCGCCGCGCCCTCCGCCGCCG CCCGGGGCCACGCCGCGCGCCCCGAGCGCACCTGCCGCCCCCGCACGCCGGCTGGCCTGAGCCCGCCGAC CTGGGCGAGGAGGAGCCCATGCTGGGCCTGGGCGAGGAGGAGGAGGAGGAGGAGACGGGGGCAGCCGAGG GCGCCGGCGAGGAAGCGGAGGAGGCAGGCGCGGAGGAGGCGTGCACTAAGGCGGTCGGCGCTGACGGCAA GGCGGCAGGGACCCCGGGCCCGACCGGGCAACACGATGGGCGGAGGCGCATCCAGCGGGAGGGCCTGATG CGCGTGTACGTGGCCCAGCTGGTGGCCAGGGCAGCTTTCGAGGTGGCCTTCCTGGTGGGCCAGTACCTGC TGTACGGCTTCGAGGTGCGACCGTTCTTTCCCTGCAGCCGCCAGCCCTGCCCGCACGTGGTGGACTGCTT CGTGTCGCGCCCTACTGAAAAGACGGTCTTCCTGCTGGTTATGTACGTGGTCAGCTGCCTGTGCCTGCTG CTCAACCTCTGTGAGATGGCCCACCTGGGCTTGGGCAGCGCGCAGGACGCGGTGCGCGGCCGCCGCGGCC CCCCGGCCTCCGCCCCCGCCCCCGCGCCGCGGCCCCCGCCCTGCGCCTTCCCTGCGGCGGCCGCTGGCTT GGCCTGCCCGCCCGACTACAGCCTGGTGGTGCGGGCGGCCGAGCGCGCTCGGGCGCATGACCAGAACCTG GCAAACCTGGCCCTGCAGGCGCTGCGCGACGGGGCAGCGGCTGGGGACCGCGACCGGGACAGTTCGCCGT GCGTCGGCCTCCCTGCGGCCTCCCGGGGGCCCCCCAGAGCAGGCGCCCCCGCGTCCCGGACGGGCAGTGC TACCTCTGCGGGCACTGTCGGGGAGCAGGGCCGGCCCGGCACCCACGAGCGGCCAGGAGCCAAGCCCAGG GCTGGCTCCGAGAAGGGCAGTGCCAGCAGCAGGGACGGGAAGACCACCGTGTGGATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG212096 representing NM_020435
Red=Cloning site Green=Tags(s) MTNMSWSFLTRLLEEIHNHSTFVGKVWLTVLVVFRIVLTAVGGEAIYSDEQAKFTCNTRQPGCDNVCYDA FAPLSHVRFWVFQIVVISTPSVMYLGYAVHRLARASEQERRRALRRRPGPRRAPRAHLPPPHAGWPEPAD LGEEEPMLGLGEEEEEEETGAAEGAGEEAEEAGAEEACTKAVGADGKAAGTPGPTGQHDGRRRIQREGLM RVYVAQLVARAAFEVAFLVGQYLLYGFEVRPFFPCSRQPCPHVVDCFVSRPTEKTVFLLVMYVVSCLCLL LNLCEMAHLGLGSAQDAVRGRRGPPASAPAPAPRPPPCAFPAAAAGLACPPDYSLVVRAAERARAHDQNL ANLALQALRDGAAAGDRDRDSSPCVGLPAASRGPPRAGAPASRTGSATSAGTVGEQGRPGTHERPGAKPR AGSEKGSASSRDGKTTVWI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020435 |
| ORF Size | 1317 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020435.4 |
| RefSeq Size | 2411 bp |
| RefSeq ORF | 1320 bp |
| Locus ID | 57165 |
| UniProt ID | Q5T442 |
| Protein Families | Ion Channels: Other |
| Gene Summary | This gene encodes a gap junction protein. Gap junction proteins are members of a large family of homologous connexins and comprise 4 transmembrane, 2 extracellular, and 3 cytoplasmic domains. This gene plays a key role in central myelination and is involved in peripheral myelination in humans. Defects in this gene are the cause of autosomal recessive Pelizaeus-Merzbacher-like disease-1. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212096 | GJC2 (Myc-DDK-tagged)-Human gap junction protein, gamma 2, 47kDa (GJC2) |
CNY 5,488.00 |
|
| RC212096L3 | Lenti-ORF clone of GJC2 (Myc-DDK-tagged)-Human gap junction protein, gamma 2, 47kDa (GJC2) |
CNY 5,890.00 |
|
| RC212096L4 | Lenti-ORF clone of GJC2 (mGFP-tagged)-Human gap junction protein, gamma 2, 47kDa (GJC2) |
CNY 5,890.00 |
|
| SC313476 | GJC2 (untagged)-Human gap junction protein, gamma 2, 47kDa (GJC2) |
CNY 3,656.00 |
