United States
Germany
Japan
United Kingdom
China
gamma Adaptin (AP1G1) (NM_001128) Human Tagged ORF Clone
CAT＃: RG216694
- TrueORF®
AP1G1 (tGFP-tagged) - Human adaptor-related protein complex 1, gamma 1 subunit (AP1G1), transcript variant 2
ORF Plasmid: DDK
"NM_001128" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 7,640.00
CNY 9,500.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ADTG; CLAPG1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG216694 representing NM_001128
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCCCCCATCAGATTGCGGGAGCTGATCCGGACCATCCGGACAGCCCGAACCCAAGCTGAAGAAC GAGAAATGATCCAGAAAGAATGTGCTGCAATCCGGTCATCTTTTAGAGAAGAAGACAATACATACCGATG TCGGAATGTGGCAAAATTACTGTATATGCACATGCTGGGCTACCCTGCTCACTTTGGACAGTTGGAGTGC CTCAAGCTTATTGCCTCTCAAAAATTTACAGACAAACGCATTGGCTATTTAGGGGCAATGCTGCTGTTAG ATGAAAGACAAGATGTCCATCTTCTCATGACCAACTGTATCAAGAATGATCTTAATCATAGCACGCAATT CGTACAGGGGTTAGCACTTTGTACCCTCGGCTGCATGGGCTCCTCAGAGATGTGCAGAGATCTTGCAGGA GAGGTAGAGAAGCTCCTGAAAACCTCCAACTCTTACTTAAGAAAAAAGGCAGCACTGTGTGCTGTTCATG TCATCAGGAAAGTTCCTGAACTTATGGAGATGTTTTTACCAGCAACAAAAAATTTATTGAATGAGAAGAA CCATGGTGTCCTCCACACATCTGTAGTCCTCCTCACAGAAATGTGTGAGCGAAGCCCAGACATGCTTGCG CATTTCAGAAAGCTTGTGCCCCAATTAGTTCGTATTTTAAAGAACCTCATCATGTCCGGATATTCACCAG AACATGATGTTTCTGGTATCAGTGACCCCTTTTTGCAGGTACGAATTTTGCGGTTATTAAGAATTTTAGG ACGAAATGATGATGATTCAAGTGAAGCTATGAATGATATATTAGCACAGGTTGCCACTAATACTGAGACT AGTAAAAATGTAGGAAATGCTATTCTTTATGAAACGGTTTTGACTATCATGGATATTAAGTCAGAGAGTG GATTGCGAGTCCTAGCCATAAATATCCTGGGTCGTTTCTTATTGAACAATGACAAGAATATTAGATATGT GGCTCTGACATCTTTGTTGAAGACTGTACAGACAGATCATAATGCAGTACAGAGGCACAGAAGCACAATT GTGGACTGTCTTAAAGATTTGGATGTCTCAATAAAACGGCGTGCAATGGAATTGAGTTTTGCCCTGGTAA ATGGGAATAATATCCGAGGCATGATGAAAGAATTACTTTATTTTCTGGATTCGTGTGAGCCAGAATTTAA AGCAGACTGTGCATCTGGAATCTTTCTTGCTGCAGAAAAGTATGCACCTTCCAAACGATGGCATATAGAC ACAATTATGCGTGTTTTGACAACGGCAGGAAGTTATGTTCGTGATGATGCAGTCCCCAATTTAATCCAGT TAATAACTAATAGTGTGGAGATGCATGCCTATACTGTCCAGCGCCTGTACAAAGCAATTCTTGGTGATTA TTCTCAACAACCTTTGGTACAAGTGGCTGCATGGTGTATAGGTGAATATGGTGATCTTCTTGTATCTGGC CAGTGTGAAGAGGAAGAGCCTATTCAGGTAACAGAGGATGAAGTGTTGGATATTTTAGAAAGTGTCCTAA TCTCTAATATGTCCACCTCTGTGACACGAGGTTATGCCCTCACTGCCATTATGAAGCTTTCCACTCGATT CACTTGTACTGTAAACCGAATTAAGAAAGTGGTTTCCATCTACGGAAGCAGCATTGATGTGGAACTCCAG CAGAGGGCAGTAGAATATAATGCACTTTTCAAGAAATATGACCACATGAGGTCTGCCCTACTTGAGAGAA TGCCTGTCATGGAAAAAGTGACCACAAATGGCCCTACTGAGATTGTGCAGACAAATGGAGAGACAGAACC AGCTCCACTAGAGACCAAACCGCCACCCTCTGGGCCACAGCCCACCAGCCAGGCCAATGATTTATTGGAT TTGTTGGGAGGAAATGACATAACACCTGTTATTCCAACTGCGCCTACAAGCAAACCATCTTCTGCTGGTG GAGAACTTCTTGATTTGCTGGGAGACATCAACCTTACAGGTGCTCCAGCTGCTGCTCCTGCCCCTGCCTC AGTCCCACAGATATCCCAGCCCCCCTTCTTGTTGGATGGGCTTTCATCACAGCCTCTCTTCAATGATATT GCTGCAGGCATCCCCTCCATCACAGCATACAGTAAGAATGGCTTGAAGATAGAATTCACCTTTGAACGGT CAAATACCAACCCCAGTGTAACAGTGATAACGATACAGGCCTCCAACAGCACAGAGCTAGATATGACGGA CTTTGTTTTCCAAGCTGCAGTACCAAAGACATTCCAGCTGCAGCTCTTGTCTCCTAGCAGCAGCATTGTC CCAGCATTTAACACGGGGACCATCACACAAGTCATTAAAGTTCTGAACCCTCAGAAGCAACAGCTGCGAA TGCGGATCAAGCTTACATATAATCACAAGGGCTCAGCAATGCAAGATCTAGCAGAGGTGAACAACTTTCC CCCTCAGTCCTGGCAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG216694 representing NM_001128
Red=Cloning site Green=Tags(s) MPAPIRLRELIRTIRTARTQAEEREMIQKECAAIRSSFREEDNTYRCRNVAKLLYMHMLGYPAHFGQLEC LKLIASQKFTDKRIGYLGAMLLLDERQDVHLLMTNCIKNDLNHSTQFVQGLALCTLGCMGSSEMCRDLAG EVEKLLKTSNSYLRKKAALCAVHVIRKVPELMEMFLPATKNLLNEKNHGVLHTSVVLLTEMCERSPDMLA HFRKLVPQLVRILKNLIMSGYSPEHDVSGISDPFLQVRILRLLRILGRNDDDSSEAMNDILAQVATNTET SKNVGNAILYETVLTIMDIKSESGLRVLAINILGRFLLNNDKNIRYVALTSLLKTVQTDHNAVQRHRSTI VDCLKDLDVSIKRRAMELSFALVNGNNIRGMMKELLYFLDSCEPEFKADCASGIFLAAEKYAPSKRWHID TIMRVLTTAGSYVRDDAVPNLIQLITNSVEMHAYTVQRLYKAILGDYSQQPLVQVAAWCIGEYGDLLVSG QCEEEEPIQVTEDEVLDILESVLISNMSTSVTRGYALTAIMKLSTRFTCTVNRIKKVVSIYGSSIDVELQ QRAVEYNALFKKYDHMRSALLERMPVMEKVTTNGPTEIVQTNGETEPAPLETKPPPSGPQPTSQANDLLD LLGGNDITPVIPTAPTSKPSSAGGELLDLLGDINLTGAPAAAPAPASVPQISQPPFLLDGLSSQPLFNDI AAGIPSITAYSKNGLKIEFTFERSNTNPSVTVITIQASNSTELDMTDFVFQAAVPKTFQLQLLSPSSSIV PAFNTGTITQVIKVLNPQKQQLRMRIKLTYNHKGSAMQDLAEVNNFPPQSWQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001128 |
| ORF Size | 2466 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001128.6 |
| RefSeq Size | 6841 bp |
| RefSeq ORF | 2469 bp |
| Locus ID | 164 |
| UniProt ID | O43747 |
| Domains | Adaptin_N, Alpha_adaptinC2 |
| Protein Pathways | Lysosome |
| Gene Summary | Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216694 | AP1G1 (Myc-DDK-tagged)-Human adaptor-related protein complex 1, gamma 1 subunit (AP1G1), transcript variant 2 |
CNY 6,040.00 |
|
| RC216694L3 | Lenti ORF clone of Human adaptor-related protein complex 1, gamma 1 subunit (AP1G1), transcript variant 2, Myc-DDK-tagged |
CNY 10,550.00 |
|
| RC216694L4 | Lenti ORF clone of Human adaptor-related protein complex 1, gamma 1 subunit (AP1G1), transcript variant 2, mGFP tagged |
CNY 8,440.00 |
|
| SC119413 | AP1G1 (untagged)-Human adaptor-related protein complex 1, gamma 1 subunit (AP1G1), transcript variant 2 |
CNY 6,032.00 |
其它gamma Adaptin产品
