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BRD8 (NM_006696) Human Tagged ORF Clone
CAT＃: RG217051
- TrueORF®
BRD8 (tGFP-tagged) - Human bromodomain containing 8 (BRD8), transcript variant 1
ORF Plasmid: DDK
"NM_006696" in other vectors (4)
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CNY 10,450.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | p120; SMAP; SMAP2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG217051 representing NM_006696
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACGGGAACGGGCAAACACAAGCTGCTAAGCACTGGCCCCACAGAGCCATGGTCCATCCGAGAGA AGCTATGTTTAGCATCTTCTGTCATGAGAAGTGGCGATCAAAATTGGGTATCAGTTAGCAGAGCAATCAA GCCCTTTGCAGAACCTGGCCGCCCTCCAGACTGGTTCTCTCAAAAACATTGTGCTTCCCAGTACTCGGAG CTTTTAGAGACCACTGAGACACCAAAACGGAAACGAGGTGAAAAGGGAGAAGTGGTGGAAACTGTTGAAG ATGTTATTGTTCGGAAATTGACTGCTGAGCGAGTTGAAGAACTAAAGAAAGTGATAAAGGAAACCCAGGA GAGATATAGACGGCTAAAGAGAGATGCAGAACTAATTCAAGCTGGACACATGGACAGCAGACTGGATGAG CTTTGCAATGACATTGCAACGAAAAAGAAATTGGAAGAAGAGGAGGCTGAAGTAAAGAGGAAGGCTACAG ATGCTGCATACCAGGCTCGTCAAGCAGTAAAAACACCCCCCCGGAGGTTACCCACTGTGATGGTTCGCTC TCCTATAGATTCTGCCTCCCCAGGAGGTGATTATCCACTTGGGGACTTGACTCCAACCACTATGGAAGAG GCTACCTCTGGGGTAACCCCCGGGACTTTGCCGAGTACCCCAGTCACCTCGTTTCCTGGGATTCCTGACA CCCTTCCTCCAGGCTCTGCACCCTTAGAAGCCCCCATGACCCCAGTAACAGATGATTCACCCCAGAAAAA GATGCTTGGACAGAAAGCAACTCCACCCCCCTCCCCTCTGCTGTCAGAGCTCTTGAAGAAGGGCAGCCTC CTGCCTACTAGCCCCAGACTGGTCAATGAGAGTGAAATGGCTGTGGCTTCTGGCCACCTGAACAGTACAG GTGTCCTCCTGGAGGTAGGCGGGGTCCTTCCCATGATACATGGTGGGGAGATACAGCAAACACCCAATAC TGTTGCAGCCTCCCCTGCTGCATCAGGTGCTCCCACTCTTTCCCGGCTTTTAGAAGCTGGTCCTACACAG TTCACCACACCTCTTGCTTCCTTCACTACTGTTGCCAGTGAGCCTCCAGTTAAACTTGTGCCACCCCCTG TAGAGTCTGTGTCCCAAGCTACCATTGTCATGATGCCTGCGCTGCCAGCACCATCCTCTGCTCCGGCTGT CTCCACTACTGAAAGTGTAGCTCCAGTGAGTCAACCCGACAACTGTGTTCCCATGGAGGCTGTGGGGGAT CCACATACTGTGACTGTTTCCATGGACAGCAGTGAAATATCCATGATCATCAATTCTATCAAAGAAGAGT GTTTTCGATCAGGGGTAGCAGAGGCTCCTGTTGGATCAAAGGCTCCCAGCATAGATGGGAAGGAAGAATT AGATCTGGCTGAGAAGATGGATATTGCTGTGTCTTACACAGGTGAAGAGCTGGATTTTGAGACTGTTGGA GACATCATTGCCATCATTGAGGACAAGGTAGATGATCATCCTGAAGTGCTGGATGTGGCAGCAGTGGAAG CAGCACTGTCATTTTGTGAAGAAAATGATGATCCTCAGTCCCTGCCTGGCCCCTGGGAGCATCCTATCCA GCAGGAGCGGGACAAGCCAGTACCTCTCCCTGCACCAGAAATGACGGTCAAGCAAGAGAGACTGGACTTT GAGGAAACGGAAAACAAGGGAATACATGAACTGGTGGACATCAGGGAGCCCAGTGCAGAGATCAAGGTGG AACCTGCAGAACCAGAGCCAGTCATTTCAGGAGCCGAAATAGTAGCTGGAGTTGTTCCAGCCACAAGTAT GGAGCCACCAGAACTCAGGAGTCAGGACTTAGATGAGGAACTGGGAAGTACTGCAGCTGGAGAGATTGTT GAAGCAGATGTTGCCATTGGGAAAGGCGATGAGACTCCACTTACAAATGTGAAGACAGAGGCATCCCCTG AAAGCATGTTGTCTCCATCACATGGCTCAAATCCCATTGAAGATCCTTTAGAGGCAGAGACTCAGCACAA GTTTGAAATGTCAGACTCATTGAAAGAAGAATCAGGGACTATTTTTGGAAGCCAGATAAAGGATGCCCCA GGTGAGGATGAGGAGGAAGATGGTGTCAGTGAAGCGGCCAGCCTAGAGGAGCCTAAGGAAGAGGATCAAG GAGAAGGCTACTTGTCAGAAATGGATAATGAACCTCCTGTGAGCGAGAGTGATGATGGCTTCAGCATACA CAATGCTACACTGCAGTCACACACACTGGCAGACTCCATCCCCAGCAGCCCTGCTTCTTCACAGTTCTCT GTCTGTAGTGAGGATCAGGAAGCTATTCAGGCACAGAAAATTTGGAAGAAAGCCATCATGCTTGTATGGA GAGCTGCAGCTAATCATAGGTATGCCAATGTCTTCCTGCAGCCTGTTACAGATGACATAGCACCTGGCTA CCACAGCATTGTGCAGAGGCCTATGGATTTGTCAACTATTAAGAAAAACATAGAAAATGGACTGATCCGA AGCACAGCTGAATTTCAGCGTGACATTATGCTGATGTTTCAGAATGCTGTAATGTACAATAGCTCAGACC ATGATGTCTATCACATGGCAGTGGAGATGCAGCGAGATGTCTTGGAACAGATCCAGCAATTCTTGGCCAC GCAGTTGATTATGCAAACATCCGAGTCTGGGATCAGTGCTAAAAGTCTTCGAGGGAGAGATTCTACCCGC AAACAGGATGCTTCAGAGAAGGACAGTGTCCCAATGGGCTCTCCTGCCTTCCTTCTCTCTCTCTTTGATG GAGGAACCAGGGGACGCCGCTGTGCCATTGAAGCAGATATGAAGATGAAAAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217051 representing NM_006696
Red=Cloning site Green=Tags(s) MATGTGKHKLLSTGPTEPWSIREKLCLASSVMRSGDQNWVSVSRAIKPFAEPGRPPDWFSQKHCASQYSE LLETTETPKRKRGEKGEVVETVEDVIVRKLTAERVEELKKVIKETQERYRRLKRDAELIQAGHMDSRLDE LCNDIATKKKLEEEEAEVKRKATDAAYQARQAVKTPPRRLPTVMVRSPIDSASPGGDYPLGDLTPTTMEE ATSGVTPGTLPSTPVTSFPGIPDTLPPGSAPLEAPMTPVTDDSPQKKMLGQKATPPPSPLLSELLKKGSL LPTSPRLVNESEMAVASGHLNSTGVLLEVGGVLPMIHGGEIQQTPNTVAASPAASGAPTLSRLLEAGPTQ FTTPLASFTTVASEPPVKLVPPPVESVSQATIVMMPALPAPSSAPAVSTTESVAPVSQPDNCVPMEAVGD PHTVTVSMDSSEISMIINSIKEECFRSGVAEAPVGSKAPSIDGKEELDLAEKMDIAVSYTGEELDFETVG DIIAIIEDKVDDHPEVLDVAAVEAALSFCEENDDPQSLPGPWEHPIQQERDKPVPLPAPEMTVKQERLDF EETENKGIHELVDIREPSAEIKVEPAEPEPVISGAEIVAGVVPATSMEPPELRSQDLDEELGSTAAGEIV EADVAIGKGDETPLTNVKTEASPESMLSPSHGSNPIEDPLEAETQHKFEMSDSLKEESGTIFGSQIKDAP GEDEEEDGVSEAASLEEPKEEDQGEGYLSEMDNEPPVSESDDGFSIHNATLQSHTLADSIPSSPASSQFS VCSEDQEAIQAQKIWKKAIMLVWRAAANHRYANVFLQPVTDDIAPGYHSIVQRPMDLSTIKKNIENGLIR STAEFQRDIMLMFQNAVMYNSSDHDVYHMAVEMQRDVLEQIQQFLATQLIMQTSESGISAKSLRGRDSTR KQDASEKDSVPMGSPAFLLSLFDGGTRGRRCAIEADMKMKK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006696 |
| ORF Size | 2853 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006696.4 |
| RefSeq Size | 3253 bp |
| RefSeq ORF | 2856 bp |
| Locus ID | 10902 |
| UniProt ID | Q9H0E9 |
| Domains | BROMO |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | The protein encoded by this gene interacts with thyroid hormone receptor in a ligand-dependent manner and enhances thyroid hormone-dependent activation from thyroid response elements. This protein contains a bromodomain and is thought to be a nuclear receptor coactivator. Multiple alternatively spliced transcript variants that encode distinct isoforms have been identified. [provided by RefSeq, Jul 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217051 | BRD8 (Myc-DDK-tagged)-Human bromodomain containing 8 (BRD8), transcript variant 1 |
CNY 9,500.00 |
|
| RC217051L3 | Lenti-ORF clone of BRD8 (Myc-DDK-tagged)-Human bromodomain containing 8 (BRD8), transcript variant 1 |
CNY 11,400.00 |
|
| RC217051L4 | Lenti-ORF clone of BRD8 (mGFP-tagged)-Human bromodomain containing 8 (BRD8), transcript variant 1 |
CNY 11,400.00 |
|
| SC310032 | BRD8 (untagged)-Human bromodomain containing 8 (BRD8), transcript variant 1 |
CNY 18,340.00 |
