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AP4E1 (NM_007347) Human Tagged ORF Clone
CAT＃: RG217100
- TrueORF®
AP4E1 (tGFP-tagged) - Human adaptor-related protein complex 4, epsilon 1 subunit (AP4E1)
ORF Plasmid: DDK
"NM_007347" in other vectors (4)
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CNY 9,220.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CPSQ4; SPG51; STUT1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG217100 representing NM_007347
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGACATAGTGGAGAAGACGCTGACGGCGCTGCCGGGACTCTTTCTGCAGAACCAGCCCGGTGGTG GGCCCGCGGCCGCCAAGGCGTCCTTCTCCTCGAGGCTGGGCAGCCTTGTCCGCGGCATCACAGCCCTCAC CTCCAAGCACGAAGAAGAAAAATTAATCCAGCAGGAACTGAGTAGTCTGAAAGCGACTGTTTCTGCTCCT ACTACAACACTGAAAATGATGAAGGAATGTATGGTGAGACTTATATATTGTGAAATGCTTGGATATGATG CTTCCTTTGGCTATATTCATGCAATCAAGTTAGCCCAACAAGGAAACCTCTTAGAAAAAAGAGTAGGTTA TTTGGCTGTTTCCTTATTTCTACATGAAAGTCATGAATTATTGCTTCTCCTTGTGAATACAGTTGTAAAG GATCTGCAGAGCACTAACCTAGTAGAAGTGTGTATGGCACTGACTGTTGTTAGCCAGATTTTCCCCTGCG AAATGATTCCAGCTGTTCTTCCATTAATAGAAGATAAACTTCAACATTCTAAGGAGATTGTACGAAGAAA AGCTGTTCTGGCATTATACAAATTCCATCTCATTGCTCCTAATCAAGTACAACATATTCATATTAAGTTT CGGAAAGCACTTTGTGACAGAGATGTTGGGGTCATGGCTGCCTCCTTGCATATATATCTTAGAATGATTA AGGAGAATTCATCTGGATATAAAGACTTGACTGGGAGTTTTGTAACCATTTTGAAGCAAGTAGTTGGAGG AAAGCTCCCAGTAGAATTCAATTACCACAGTGTGCCAGCACCATGGTTACAAATTCAGCTCTTGAGAATA CTGGGACTTCTAGGAAAAGATGATCAAAGGACAAGTGAATTAATGTATGATGTTCTTGATGAATCCTTAC GAAGAGCTGAGTTAAATCACAATGTCACATATGCTATTTTGTTTGAATGTGTGCATACAGTCTATTCTAT TTATCCTAAATCGGAATTACTTGAGAAGGCTGCCAAGTGCATTGGAAAATTTGTTCTGTCACCTAAAATA AATCTAAAATATTTAGGACTGAAGGCTCTTACCTATGTTATCCAACAGGATCCTACTCTGGCTCTTCAAC ACCAGATGACAATAATTGAATGTTTAGATCATCCTGATCCCATTATTAAAAGAGAGACTCTGGAACTTCT TTACAGAATTACTAATGCACAGAATATAACAGTTATTGTCCAGAAAATGCTTGAATATTTACATCAGAGC AAAGAAGAGTATGTCATCGTCAATTTGGTCGGCAAAATAGCAGAGCTGGCTGAGAAATATGCTCCTGATA ATGCATGGTTTATTCAGACAATGAATGCTGTGTTTTCAGTAGGAGGAGATGTAATGCATCCTGATATTCC CAATAACTTTCTGAGACTACTAGCGGAAGGTTTTGATGATGAAACAGAAGATCAGCAATTAAGACTCTAT GCAGTTCAGTCTTATCTCACTTTACTGGATATGGAAAATGTGTTCTATCCACAGAGATTTCTTCAAGTTA TGAGTTGGGTATTAGGGGAATATTCCTACCTCTTAGATAAGGAAACGCCAGAGGAAGTTATAGCTAAGCT CTACAAGTTACTTATGAATGACTCTGTGTCTTCAGAAACAAAAGCCTGGTTAATTGCTGCTGTGACCAAA TTGACATCTCAGGCGCACTCTTCTAATACAGTTGAGAGATTAATCCATGAATTTACCATATCTTTGGATA CTTGTATGAGACAACATGCATTTGAATTAAAACATTTGCATGAGAATGTGGAACTTATGAAGAGCTTGCT TCCAGTTGACAGGAGTTGTGAAGACTTGGTGGTAGATGCTTCTTTATCTTTTCTGGATGGTTTTGTGGCT GAAGGACTCAGTCAGGGTGCAGCGCCTTACAAACCTCCCCATCAACGCCAGGAGGAAAAGCTTTCTCAGG AAAAAGTTCTCAATTTTGAACCATATGGACTCTCCTTTTCTTCATCTGGCTTCACTGGACGACAGTCTCC TGCTGGCATTTCTCTTGGTTCAGATGTATCTGGGAATAGTGCTGAGACAGGACTGAAAGAGACAAATAGC TTGAAGCTGGAAGGTATAAAGAAATTGTGGGGGAAAGAAGGCTATCTTCCCAAGAAGGAAAGCAAAACTG GTGATGAAAGTGGAGCTCTGCCTGTTCCTCAAGAGAGTATAATGGAGAATGTAGATCAAGCTATAACTAA AAAGGATCAATCTCAAGTTCTTACCCAATCTAAAGAGGAGAAAGAAAAGCAGCTGCTGGCATCATCATTA TTTGTTGGTCTAGGATCAGAAAGTACAATCAACCTGCTGGGAAAAGCAGATACTGTCTCTCACAAGTTCA GAAGGAAATCAAAAGTCAAAGAAGCTAAAAGTGGCGAAACAACCAGTACTCATAATATGACCTGTTCTTC CTTTAGTTCTTTGTCAAATGTGGCATATGAAGATGATTATTATTCGAATACTTTGCACGATACAGGAGAC AAGGAATTAAAGAAATTTTCTCTCACTTCAGAACTTTTGGATTCTGAGTCACTCACAGAACTGCCCTTGG TTGAGAAATTCTCATATTGTAGTCTGTCTACACCTTCATTGTTTGCTAATAACAACATGGAAATTTTTCA CCCTCCTCAATCTACTGCAGCCTCAGTTGCCAAGGAAAGCTCTTTAGCTTCATCTTTTTTGGAAGAAACT ACTGAATACATACACTCAAATGCTATGGAAGTCTGTAATAATGAAACTATATCAGTGTCTTCTTATAAAA TTTGGAAAGATGATTGTTTATTGATGGTCTGGTCAGTCACTAATAAGAGTGGTTTGGAATTGAAAAGTGC TGACTTAGAAATTTTTCCTGCAGAAAATTTCAAGGTGACTGAGCAACCTGGATGCTGTTTGCCTGTAATG GAAGCAGAAAGCACCAAAAGCTTTCAATATAGTGTGCAGATAGAAAAACCTTTTACAGAAGGAAATCTTA CTGGTTTTATTAGTTATCATATGATGGATACTCATTCTGCTCAGCTGGAATTTTCTGTAAACTTATCACT ATTAGATTTCATTAGACCATTAAAAATCTCAAGTGACGACTTTGGGAAACTCTGGTTATCCTTCGCAAAT GATGTGAAACAAAATGTAAAAATGTCAGAATCTCAAGCTGCACTTCCTTCTGCACTAAAGACTCTGCAAC AGAAACTAAGACTCCATATTATTGAGATTATAGGCAATGAAGGGCTATTGGCCTGTCAGCTGCTCCCATC CATCCCCTGCTTACTGCATTGCCGAGTTCATGCAGATGTATTAGCCCTGTGGTTCAGATCCTCCTGTTCT ACTCTTCCTGACTATTTACTGTATCAGTGTCAAAAGGTGATGGAGGGATCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217100 representing NM_007347
Red=Cloning site Green=Tags(s) MSDIVEKTLTALPGLFLQNQPGGGPAAAKASFSSRLGSLVRGITALTSKHEEEKLIQQELSSLKATVSAP TTTLKMMKECMVRLIYCEMLGYDASFGYIHAIKLAQQGNLLEKRVGYLAVSLFLHESHELLLLLVNTVVK DLQSTNLVEVCMALTVVSQIFPCEMIPAVLPLIEDKLQHSKEIVRRKAVLALYKFHLIAPNQVQHIHIKF RKALCDRDVGVMAASLHIYLRMIKENSSGYKDLTGSFVTILKQVVGGKLPVEFNYHSVPAPWLQIQLLRI LGLLGKDDQRTSELMYDVLDESLRRAELNHNVTYAILFECVHTVYSIYPKSELLEKAAKCIGKFVLSPKI NLKYLGLKALTYVIQQDPTLALQHQMTIIECLDHPDPIIKRETLELLYRITNAQNITVIVQKMLEYLHQS KEEYVIVNLVGKIAELAEKYAPDNAWFIQTMNAVFSVGGDVMHPDIPNNFLRLLAEGFDDETEDQQLRLY AVQSYLTLLDMENVFYPQRFLQVMSWVLGEYSYLLDKETPEEVIAKLYKLLMNDSVSSETKAWLIAAVTK LTSQAHSSNTVERLIHEFTISLDTCMRQHAFELKHLHENVELMKSLLPVDRSCEDLVVDASLSFLDGFVA EGLSQGAAPYKPPHQRQEEKLSQEKVLNFEPYGLSFSSSGFTGRQSPAGISLGSDVSGNSAETGLKETNS LKLEGIKKLWGKEGYLPKKESKTGDESGALPVPQESIMENVDQAITKKDQSQVLTQSKEEKEKQLLASSL FVGLGSESTINLLGKADTVSHKFRRKSKVKEAKSGETTSTHNMTCSSFSSLSNVAYEDDYYSNTLHDTGD KELKKFSLTSELLDSESLTELPLVEKFSYCSLSTPSLFANNNMEIFHPPQSTAASVAKESSLASSFLEET TEYIHSNAMEVCNNETISVSSYKIWKDDCLLMVWSVTNKSGLELKSADLEIFPAENFKVTEQPGCCLPVM EAESTKSFQYSVQIEKPFTEGNLTGFISYHMMDTHSAQLEFSVNLSLLDFIRPLKISSDDFGKLWLSFAN DVKQNVKMSESQAALPSALKTLQQKLRLHIIEIIGNEGLLACQLLPSIPCLLHCRVHADVLALWFRSSCS TLPDYLLYQCQKVMEGS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007347 |
| ORF Size | 3411 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007347.5 |
| RefSeq Size | 6768 bp |
| RefSeq ORF | 3414 bp |
| Locus ID | 23431 |
| UniProt ID | Q9UPM8 |
| Protein Pathways | Lysosome |
| Gene Summary | This gene encodes a member of the adaptor complexes large subunit protein family. These proteins are components of the heterotetrameric adaptor protein complexes, which play important roles in the secretory and endocytic pathways by mediating vesicle formation and sorting of integral membrane proteins. The encoded protein is a large subunit of adaptor protein complex-4, which is associated with both clathrin- and nonclathrin-coated vesicles. Disruption of this gene may be associated with cerebral palsy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217100 | AP4E1 (Myc-DDK-tagged)-Human adaptor-related protein complex 4, epsilon 1 subunit (AP4E1) |
CNY 7,920.00 |
|
| RC217100L1 | Lenti-ORF clone of AP4E1 (Myc-DDK-tagged)-Human adaptor-related protein complex 4, epsilon 1 subunit (AP4E1) |
CNY 10,260.00 |
|
| RC217100L2 | Lenti-ORF clone of AP4E1 (mGFP-tagged)-Human adaptor-related protein complex 4, epsilon 1 subunit (AP4E1) |
CNY 10,260.00 |
|
| SC303918 | AP4E1 (untagged)-Human adaptor-related protein complex 4, epsilon 1 subunit (AP4E1) |
CNY 7,928.00 |
