United States
Germany
Japan
United Kingdom
China
DOCK1 (NM_001380) Human Tagged ORF Clone
CAT＃: RG219945
- TrueORF®
DOCK1 (tGFP-tagged) - Human dedicator of cytokinesis 1 (DOCK1)
ORF Plasmid: DDK
"NM_001380" in other vectors (2)
Need custom modification / cloning service?
Get a free quote
CNY 20,520.00
货期*
3周
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ced5; DOCK180 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG219945 representing NM_001380
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGCGCTGGGTGCCCACCAAGCGCGAGGAGAAGTACGGCGTGGCTTTTTATAACTATGATGCCAGAG GAGCGGATGAACTTTCTTTACAGATCGGAGACACTGTGCACATCTTAGAAACATATGAAGGGTGGTACCG AGGTTACACGTTACGAAAAAAGTCTAAGAAGGGTATATTTCCTGCTTCATATATTCATCTTAAAGAAGCG ATAGTTGAAGGAAAAGGGCAACATGAAACAGTCATCCCGGGTGACCTCCCCCTCATCCAGGAAGTCACCA CGACACTCCGAGAGTGGTCCACCATCTGGAGGCAGCTCTACGTGCAAGATAACAGGGAGATGTTTCGAAG TGTGCGGCACATGATCTATGACCTTATTGAATGGCGATCACAAATTCTTTCTGGAACTCTGCCTCAGGAT GAACTCAAAGAACTGAAGAAGAAGGTCACAGCCAAAATTGATTATGGAAACAGAATTCTAGATTTGGACC TGGTGGTTAGAGATGAAGATGGGAATATTTTGGATCCAGAATTAACTAGCACGATTAGTCTCTTCAGAGC TCATGAAATAGCTTCTAAACAAGTGGAGGAAAGGTTACAAGAGGAAAAATCTCAAAAGCAGAACATAGAT ATTAACAGACAAGCCAAGTTTGCTGCAACCCCTTCTCTGGCCTTGTTTGTGAACCTCAAAAATGTGGTTT GTAAAATAGGAGAAGATGCTGAAGTCCTCATGTCTCTATATGACCCTGTGGAGTCCAAATTCATCAGTGA GAACTACCTGGTTCGCTGGTCCAGTTCAGGATTACCTAAAGACATAGACAGATTACATAATTTGCGAGCC GTGTTTACTGACCTCGGAAGCAAAGACCTGAAAAGGGAGAAAATCAGTTTTGTCTGTCAGATTGTTCGCG TGGGTCGCATGGAGCTGAGGGACAACAACACCAGGAAACTGACCTCGGGGTTGCGGCGACCTTTTGGAGT GGCTGTGATGGATGTAACAGATATAATAAATGGAAAAGTAGATGATGAAGATAAGCAGCATTTCATTCCC TTTCAGCCGGTGGCAGGGGAGAATGACTTCCTTCAGACTGTTATAAACAAAGTCATCGCTGCCAAAGAAG TCAACCACAAGGGGCAGGGTTTGTGGGTAACATTGAAATTACTTCCTGGAGATATCCATCAGATCCGAAA AGAGTTTCCGCATTTAGTGGACAGGACCACAGCTGTGGCTCGAAAAACAGGGTTTCCGGAGATAATCATG CCTGGTGATGTTCGAAATGATATCTATGTAACATTAGTTCAAGGAGATTTTGATAAAGGAAGCAAAACAA CAGCGAAGAACGTGGAGGTCACGGTGTCTGTGTACGATGAGGATGGGAAACGATTAGAGCATGTGATTTT CCCGGGTGCTGGTGATGAAGCGATTTCAGAGTACAAATCTGTGATTTACTACCAAGTAAAGCAGCCACGC TGGTTTGAGACTGTTAAGGTGGCCATTCCCATCGAGGACGTTAACCGCAGTCACCTTCGGTTTACCTTCC GCCACAGGTCATCACAGGACTCTAAGGATAAATCTGAGAAAATATTTGCACTAGCATTTGTCAAGCTGAT GAGATACGATGGTACCACCCTGCGAGACGGAGAGCACGATCTTATCGTCTATAAGGCCGAAGCAAAGAAG CTGGAAGATGCTGCCACGTACTTGAGTCTGCCCTCCACGAAGGCAGAGTTGGAAGAAAAGGGCCACTCGG CCACCGGCAAGAGCATGCAGAGCCTTGGGAGCTGCACCATTAGCAAGGACTCCTTCCAGATCTCCACGCT CGTGTGCTCCACCAAACTGACTCAGAACGTGGACCTTCTGGGGCTCTTGAAATGGCGCTCCAACACCAGC CTGCTGCAGCAGAACTTGAGGCAGCTGATGAAAGTCGATGGTGGTGAAGTAGTGAAGTTTCTTCAGGACA CGTTGGATGCCCTCTTCAACATCATGATGGAGAACTCAGAGAGTGAGACTTTTGACACGTTAGTCTTTGA TGCTCTGGTATTTATCATTGGACTGATTGCTGATAGAAAATTTCAGCATTTTAATCCTGTTTTGGAAACT TACATTAAGAAACACTTTAGTGCAACGTTAGCCTACACGAAGTTGACAAAAGTGTTGAAGAACTACGTGG ACGGTGCTGAGAAGCCGGGAGTAAATGAGCAGCTGTACAAAGCCATGAAAGCGCTAGAATCCATCTTCAA GTTCATCGTGCGCTCCAGGATCCTGTTCAATCAACTGTATGAAAACAAGGGAGAGGCTGACTTCGTGGAA TCTTTGCTGCAGCTCTTCAGGTCCATCAATGACATGATGAGCAGCATGTCAGACCAGACCGTCCGGGTGA AGGGGGCAGCACTGAAATACTTACCAACGATCGTCAACGATGTGAAATTGGTGTTTGATCCCAAAGAGCT CAGCAAAATGTTTACTGAATTCATCCTCAATGTTCCCATGGGCTTGCTGACCATCCAGAAACTCTACTGC TTGATCGAAATCGTCCACAGTGACCTCTTCACACAGCATGACTGCAGAGAGATCCTGCTTCCCATGATGA CCGATCAGCTCAAGTACCATCTGGAGAGACAGGAGGACCTGGAGGCCTGCTGTCAGCTGCTCAGCCACAT CCTGGAGGTGCTGTACAGGAAGGACGTGGGGCCAACCCAGAGGCACGTCCAGATTATCATGGAGAAACTT CTCCGGACCGTGAACCGAACCGTCATTTCCATGGGACGAGATTCTGAACTCATTGGAAACTTCGTGGCTT GCATGACAGCTATTTTACGACAAATGGAAGATTACCATTATGCCCACTTGATCAAGACTTTTGGGAAAAT GAGGACTGATGTGGTAGATTTCCTAATGGAAACATTCATCATGTTTAAGAACCTCATTGGAAAGAACGTT TACCCCTTCGACTGGGTGATCATGAACATGGTGCAAAATAAAGTCTTCCTGCGAGCAATTAATCAGTATG CAGATATGCTGAACAAAAAATTTCTGGATCAAGCCAACTTTGAGCTACAGCTGTGGAACAACTACTTTCA CCTGGCTGTTGCTTTCCTTACTCAAGAGTCCCTGCAACTGGAGAATTTTTCAAGTGCCAAGAGAGCCAAA ATCCTTAACAAGTACGGAGATATGAGGAGACAGATTGGCTTTGAAATCAGAGACATGTGGTACAACCTTG GTCAACACAAGATAAAGTTCATTCCAGAAATGGTGGGCCCAATATTAGAAATGACATTAATTCCCGAGAC GGAGCTGCGCAAAGCCACCATCCCCATCTTCTTTGATATGATGCAGTGTGAATTCCATTCGACCCGAAGC TTCCAAATGTTTGAAAATGAGATCATCACCAAGCTGGATCATGAAGTCGAAGGAGGCAGAGGAGACGAAC AGTACAAAGTGTTATTTGATAAAATCCTTCTGGAACACTGCAGGAAGCACAAATACCTCGCCAAAACAGG AGAAACTTTTGTAAAACTCGTTGTGCGCTTAATGGAAAGGCTTTTGGATTATAGAACCATCATGCACGAC GAGAACAAAGAAAACCGCATGAGCTGCACCGTCAATGTGCTGAATTTCTACAAAGAAATTGAAAGAGAAG AAATGTATATAAGGTATTTGTACAAGCTCTGTGACCTGCACAAGGAGTGTGATAACTACACCGAAGCGGC TTACACCTTGCTTCTCCATGCAAAGCTTCTTAAGTGGTCGGAGGATGTGTGTGTGGCCCACCTCACCCAG CGGGACGGGTACCAGGCCACCACGCAGGGACAGCTGAAGGAGCAGCTCTACCAGGAAATCATCCACTACT TCGACAAAGGCAAGATGTGGGAGGAGGCCATTGCCTTGGGCAAGGAGCTAGCCGAGCAGTATGAGAACGA AATGTTTGATTATGAGCAACTCAGCGAATTGCTGAAAAAACAGGCTCAGTTTTATGAAAACATCGTCAAA GTGATCAGGCCCAAGCCTGACTATTTTGCTGTTGGCTACTACGGACAAGGGTTCCCCACATTCCTGCGGG GAAAAGTTTTCATTTACCGAGGGAAAGAGTATGAGCGCCGGGAAGATTTTGAGGCTCGGCTCTTAACTCA GTTTCCAAACGCCGAGAAAATGAAGACAACATCTCCACCAGGCGACGATATTAAAAACTCTCCTGGCCAG TATATTCAGTGCTTCACAGTGAAGCCCAAACTCGATCTGCCTCCTAAGTTTCACAGGCCAGTGTCAGAGC AGATTGTAAGTTTTTACAGGGTGAACGAGGTCCAGCGATTTGAATATTCTCGGCCAATCCGGAAGGGAGA GAAAAACCCAGACAATGAATTTGCGAATATGTGGATCGAGAGAACCATATATACAACTGCATATAAATTA CCTGGAATTTTAAGGTGGTTTGAGGTCAAGTCTGTTTTCATGGTGGAAATCAGCCCCCTGGAGAATGCCA TTGAGACCATGCAGCTGACGAACGACAAGATCAACAGCATGGTGCAGCAGCACCTGGATGACCCCAGCCT GCCCATCAACCCGCTCTCCATGCTCCTGAACGGCATCGTGGACCCAGCTGTCATGGGGGGCTTCGCAAAC TACGAAAAGGCCTTCTTTACAGACCGGTACCTGCAGGAGCACCCTGAGGCCCATGAAAAGATCGAGAAGC TCAAGGACCTGATTGCTTGGCAGATTCCTTTTCTGGCCGAAGGGATCAGAATCCATGGAGACAAAGTCAC GGAGGCACTGAGGCCGTTCCACGAGAGGATGGAGGCCTGTTTCAAACAGCTGAAGGAAAAGGTGGAGAAA GAGTACGGCGTCCGAATCATGCCCTCAAGTCTGGATGATAGAAGAGGCAGCCGCCCCCGGTCCATGGTGC GGTCCTTCACGATGCCTTCCTCATCCCGCCCTCTGTCTGTGGCCTCTGTCTCTTCCCTCTCATCGGACAG CACCCCTTCCAGACCAGGCTCCGACGGGTTTGCCCTGGAGCCTCTCCTGCCAAAGAAAATGCACTCCAGG TCCCAGGACAAGCTGGACAAGGATGACCTGGAGAAGGAGAAGAAGGACAAGAAGAAGGAAAAAAGGAACA GCAAACATCAAGAGATATTTGAGAAAGAATTTAAACCCACCGACATTTCCCTGCAGCAGTCTGAGGCTGT GATCCTTTCGGAAACGATAAGTCCCCTGCGGCCCCAGAGACCGAAGAGCCAGGTGATGAACGTCATTGGA AGCGAAAGGCGCTTCTCGGTGTCCCCCTCGTCACCGTCCTCCCAGCAAACACCCCCTCCAGTTACACCAA GGGCCAAGCTCAGCTTCAGCATGCAGTCGAGCTTGGAGCTGAACGGCATGACGGGGGCGGACGTGGCCGA TGTCCCACCCCCTCTGCCTCTCAAAGGCAGCGTGGCAGATTACGGGAATTTGATGGAAAACCAGGACTTG CTGGGCTCGCCAACACCTCCACCTCCCCCTCCACACCAGAGGCATCTGCCACCTCCACTGCCCAGCAAAA CTCCGCCTCCTCCCCCTCCAAAGACAACTCGCAAGCAGACATCGGTGGACTCCGGGATCGTGCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG219945 representing NM_001380
Red=Cloning site Green=Tags(s) MTRWVPTKREEKYGVAFYNYDARGADELSLQIGDTVHILETYEGWYRGYTLRKKSKKGIFPASYIHLKEA IVEGKGQHETVIPGDLPLIQEVTTTLREWSTIWRQLYVQDNREMFRSVRHMIYDLIEWRSQILSGTLPQD ELKELKKKVTAKIDYGNRILDLDLVVRDEDGNILDPELTSTISLFRAHEIASKQVEERLQEEKSQKQNID INRQAKFAATPSLALFVNLKNVVCKIGEDAEVLMSLYDPVESKFISENYLVRWSSSGLPKDIDRLHNLRA VFTDLGSKDLKREKISFVCQIVRVGRMELRDNNTRKLTSGLRRPFGVAVMDVTDIINGKVDDEDKQHFIP FQPVAGENDFLQTVINKVIAAKEVNHKGQGLWVTLKLLPGDIHQIRKEFPHLVDRTTAVARKTGFPEIIM PGDVRNDIYVTLVQGDFDKGSKTTAKNVEVTVSVYDEDGKRLEHVIFPGAGDEAISEYKSVIYYQVKQPR WFETVKVAIPIEDVNRSHLRFTFRHRSSQDSKDKSEKIFALAFVKLMRYDGTTLRDGEHDLIVYKAEAKK LEDAATYLSLPSTKAELEEKGHSATGKSMQSLGSCTISKDSFQISTLVCSTKLTQNVDLLGLLKWRSNTS LLQQNLRQLMKVDGGEVVKFLQDTLDALFNIMMENSESETFDTLVFDALVFIIGLIADRKFQHFNPVLET YIKKHFSATLAYTKLTKVLKNYVDGAEKPGVNEQLYKAMKALESIFKFIVRSRILFNQLYENKGEADFVE SLLQLFRSINDMMSSMSDQTVRVKGAALKYLPTIVNDVKLVFDPKELSKMFTEFILNVPMGLLTIQKLYC LIEIVHSDLFTQHDCREILLPMMTDQLKYHLERQEDLEACCQLLSHILEVLYRKDVGPTQRHVQIIMEKL LRTVNRTVISMGRDSELIGNFVACMTAILRQMEDYHYAHLIKTFGKMRTDVVDFLMETFIMFKNLIGKNV YPFDWVIMNMVQNKVFLRAINQYADMLNKKFLDQANFELQLWNNYFHLAVAFLTQESLQLENFSSAKRAK ILNKYGDMRRQIGFEIRDMWYNLGQHKIKFIPEMVGPILEMTLIPETELRKATIPIFFDMMQCEFHSTRS FQMFENEIITKLDHEVEGGRGDEQYKVLFDKILLEHCRKHKYLAKTGETFVKLVVRLMERLLDYRTIMHD ENKENRMSCTVNVLNFYKEIEREEMYIRYLYKLCDLHKECDNYTEAAYTLLLHAKLLKWSEDVCVAHLTQ RDGYQATTQGQLKEQLYQEIIHYFDKGKMWEEAIALGKELAEQYENEMFDYEQLSELLKKQAQFYENIVK VIRPKPDYFAVGYYGQGFPTFLRGKVFIYRGKEYERREDFEARLLTQFPNAEKMKTTSPPGDDIKNSPGQ YIQCFTVKPKLDLPPKFHRPVSEQIVSFYRVNEVQRFEYSRPIRKGEKNPDNEFANMWIERTIYTTAYKL PGILRWFEVKSVFMVEISPLENAIETMQLTNDKINSMVQQHLDDPSLPINPLSMLLNGIVDPAVMGGFAN YEKAFFTDRYLQEHPEAHEKIEKLKDLIAWQIPFLAEGIRIHGDKVTEALRPFHERMEACFKQLKEKVEK EYGVRIMPSSLDDRRGSRPRSMVRSFTMPSSSRPLSVASVSSLSSDSTPSRPGSDGFALEPLLPKKMHSR SQDKLDKDDLEKEKKDKKKEKRNSKHQEIFEKEFKPTDISLQQSEAVILSETISPLRPQRPKSQVMNVIG SERRFSVSPSSPSSQQTPPPVTPRAKLSFSMQSSLELNGMTGADVADVPPPLPLKGSVADYGNLMENQDL LGSPTPPPPPPHQRHLPPPLPSKTPPPPPPKTTRKQTSVDSGIVQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001380 |
| ORF Size | 5595 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001380.4 |
| RefSeq Size | 6751 bp |
| RefSeq ORF | 5598 bp |
| Locus ID | 1793 |
| UniProt ID | Q14185 |
| Domains | SH3 |
| Protein Families | Druggable Genome |
| Protein Pathways | Focal adhesion, Regulation of actin cytoskeleton |
| Gene Summary | This gene encodes a member of the dedicator of cytokinesis protein family. Dedicator of cytokinesis proteins act as guanine nucleotide exchange factors for small Rho family G proteins. The encoded protein regulates the small GTPase Rac, thereby influencing several biological processes, including phagocytosis and cell migration. Overexpression of this gene has also been associated with certain cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
You may also need
Customer
Reviews
Loading...
