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TTC3 (NM_003316) Human Tagged ORF Clone
CAT＃: RG221541
- TrueORF®
TTC3 (tGFP-tagged) - Human tetratricopeptide repeat domain 3 (TTC3), transcript variant 1
ORF Plasmid: DDK
"NM_003316" in other vectors (3)
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CNY 20,900.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DCRR1; RNF105; TPRDIII |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG221541 representing NM_003316
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAATTTTGCTGAGGGAGATTTCACTGTGGCGGATTATGCCTTGTTAGAAGATTGCCCTCACGTGG ATGATTGTGTCTTTGCTGCTGAATTTATGAGCAATGATTATGTTCGTGTGACTCAGCTTTACTGTGATGG GGTGGGTGTGCAATATAAAGATTATATCCAAAGTGAGAGGAATTTGGAATTTGACATCTGCAGTATATGG TGTAGTAAACCAATTTCTGTCCTGCAAGATTATTGCGATGCCATTAAAATAAACATCTTCTGGCCACTTC TGTTTCAACATCAAAACAGTTCCGTAATATCACGATTGCATCCCTGTGTGGACGCCAACAATTCACGTGC TTCTGAGATAAATTTGAAGAAACTACAACATCTTGAGTTGATGGAAGATATTGTGGATTTGGCAAAGAAA GTTGCTAATGATTCATTCCTTATTGGAGGCTTATTGAGAATTGGTTGTAAAATAGAAAATAAAATCTTGG CAATGGAAGAAGCTCTGAATTGGATAAAATATGCAGGCGATGTAACAATTCTAACTAAATTAGGATCAAT TGACAATTGTTGGCCTATGTTAAGTATTTTCTTTACTGAATACAAGTACCACATAACTAAAATTGTAATG GAAGACTGCAATTTGCTTGAAGAACTTAAAACTCAAAGTTGTATGGATTGTATAGAGGAAGGAGAACTAA TGAAAATGAAAGGAAATGAAGAGTTTTCCAAAGAAAGATTTGATATAGCTATTATCTATTACACCAGAGC CATTGAATATAGACCTGAAAACTACCTTCTTTATGGTAACCGAGCTCTTTGTTTTCTTCGTACTGGACAG TTTAGAAATGCACTCGGTGATGGAAAGAGAGCCACTATTCTGAAGAACACTTGGCCAAAGGGTCATTATC GTTATTGTGATGCTCTTTCTATGCTGGGGGAATATGACTGGGCCCTGCAAGCAAACATAAAAGCTCAAAA ACTCTGTAAAAATGACCCTGAGGGAATCAAGGATCTAATTCAGCAGCATGTAAAGTTACAAAAACAAATA GAAGACCTACAAGGTCGAACAGCAAATAAGGATCCAATTAAAGCCTTTTATGAAAACAGGGCCTACACAC CTAGGAGTTTATCAGCACCTATATTTACTACTTCACTTAACTTTGTGGAGAAGGAAAGAGATTTCAGAAA AATTAATCACGAAATGGCCAACGGTGGTAATCAGAATCTAAAGGTGGCGGATGAGGCGTTGAAGGTAGAT GATTGTGACTGTCATCCTGAATTTTCACCACCATCAAGTCAGCCTCCAAAACATAAAGGAAAACAAAAAT CTCGAAACAATGAATCAGAAAAGTTCAGTTCTAGTTCACCATTGACTTTACCAGCAGATTTGAAGAACAT CTTGGAGAAACAGTTTTCTAAATCTTCCAGAGCTGCACACCAGGATTTTGCTAATATAATGAAAATGCTG AGAAGCTTAATTCAAGATGGCTATATGGCCTTATTGGAGCAGCGTTGCCGCAGCGCTGCACAGGCCTTTA CAGAGTTGCTGAACGGTTTAGATCCTCAAAAAATAAAGCAATTGAACCTGGCCATGATTAACTATGTTTT GGTCGTCTATGGACTTGCCATTTCTCTCCTTGGAATAGGACAGCCTGAGGAATTATCTGAAGCCGAAAAC CAGTTTAAGAGGATTATTGAACACTACCCCAGTGAGGGCCTTGATTGCTTGGCCTACTGTGGAATTGGAA AAGTATATTTGAAAAAAAACAGATTTCTAGAAGCTCTCAATCACTTTGAGAAAGCAAGAACCTTGATTTA TCGTCTTCCTGGAGTGTTAACTTGGCCCACGAGTAATGTGATTATTGAAGAGTCTCAGCCACAAAAAATA AAGATGCTGTTAGAGAAATTTGTTGAAGAATGCAAGTTCCCTCCAGTGCCAGATGCCATTTGTTGCTATC AGAAGTGCCATGGATATTCTAAGATCCAGATATACATAACTGATCCAGACTTTAAGGGTTTTATACGCAT CAGCTGTTGCCAGTACTGTAAAATAGAATTTCACATGAATTGCTGGAAGAAGTTAAAAACTACAACCTTT AATGATAAAATTGACAAGGATTTTCTACAAGGAATATGTCTTACCCCTGACTGTGAAGGTGTCATTTCTA AGATTATCATCTTCAGCAGTGGTGGTGAAGTTAAATGTGAATTTGAACACAAGGTCATAAAAGAAAAGGT TCCTCCAAGACCTATTCTGAAACAGAAATGTTCTAGCCTAGAGAAACTAAGACTGAAAGAAGACAAAAAA TTGAAGAGAAAGATCCAAAAAAAAGAAGCAAAAAAGTTAGCACAAGAAAGAATGGAGGAGGACTTAAGAG AAAGTAATCCACCCAAAAATGAAGAGCAGAAAGAAACTGTAGACAATGTTCAGCGTTGTCAGTTCCTTGA TGACAGAATTCTACAGTGTATAAAGCAGTATGCTGACAAGATTAAATCCGGCATACAGAATACAGCCATG CTTCTCAAAGAATTGCTTTCTTGGAAAGTTTTGAGCACAGAAGACTATACAACCTGTTTTTCTAGCAGAA ATTTTCTAAATGAAGCAGTGGACTATGTTATTCGCCACTTGATTCAAGAAAATAACAGAGTAAAGACAAG AATATTTCTGCATGTTTTGAGTGAGCTTAAAGAAGTGGAGCCCAAATTAGCCGCCTGGATCCAAAAACTT AATAGCTTTGGCTTAGATGCCACAGGAACTTTCTTTTCTCGTTATGGAGCATCTCTTAAACTGCTTGATT TTAGTATCATGACTTTCCTCTGGAATGAGAAATATGGTCACAAACTAGACTCTATAGAAGGAAAGCAACT TGATTATTTCTCTGAGCCAGCATCATTGAAGGAAGCCCGTTGTTTAATATGGCTGCTAGAAGAACACAGA GACAAGTTCCCAGCATTGCATAGTGCTTTAGATGAATTCTTTGATATAATGGACAGCCGCTGTACTGTGT TAAGGAAACAAGATAGTGGTGAAGCACCGTTTAGTTCAACCAAGGTGAAAAACAAAAGCAAGAAAAAGAA GCCAAAGGATTCAAAGCCTATGTTAGTTGGGTCTGGAACAACTTCAGTAACTTCAAATAATGAGATCATC ACTTCAAGTGAAGACCATAGCAATCGAAATTCAGATTCTGCAGGCCCATTTGCAGTGCCTGACCATCTTC GGCAAGATGTAGAAGAATTCGAAGCTCTCTATGACCAACACAGTAACGAATATGTTGTCCGCAATAAGAA GCTATGGGACATGAACCCAAAACAAAAATGTTCAACTCTATATGATTACTTCTCTCAGTTTTTGGAGGAA CATGGTCCCTTGGACATGAGTAACAAGATGTTCTCTGCAGAATATGAGTTTTTCCCAGAAGAAACTCGAC AGATACTAGAAAAAGCAGGAGGTTTAAAACCTTTTCTCTTGGGATGCCCTCGTTTTGTTGTGATTGACAA CTGTATTGCACTGAAGAAGGTTGCATCACGGCTCAAGAAAAAAAGGAAGAAGAAAAACATTAAAACAAAA GTAGAAGAAATTTCAAAAGCAGGGGAGTATGTACGAGTTAAACTACAACTGAATCCAGCTGCTAGGGAAT TTAAACCAGATGTAAAGTCTAAACCAGTGTCAGATTCATCTTCAGCACCAGCTTTTGAAAATGTGAAACC CAAACCTGTGTCTGCAAATTCTCCCAAGCCAGCTTGTGAAGATGTGAAGGCCAAACCAGTATCCGACAAT TCTTCTAGACAAGTTTCTGAGGATGGGCAACCCAAAGGGGTCTCTTCTAATTCTCCTAAACCAGGCTCTG AGGATGCAAATTACAAGCGAGTCTCCTGTAATTCCCCCAAACCGGTTCTTGAGGATGTGAAACCAACTTA TTGGGCTCAATCCCATTTGGTCACAGGATACTGTACGTATCTTCCTTTCCAGAGATTTGATATCACCCAG ACACCGCCAGCATACATAAACGTGTTACCAGGTTTGCCCCAGTACACCAGCATATATACACCCTTGGCCA GCCTTTCTCCTGAATATCAGCTACCAAGATCAGTACCAGTGGTGCCGTCTTTTGTAGCCAATGACAGAGC AGATAAAAATGCTGCTGCCTATTTTGAGGGTCATCATTTGAATGCTGAGAATGTTGCTGGTCACCAGATT GCCTCTGAAACACAGATCCTTGAGGGCTCTTTGGGAATATCTGTAAAGTCACACTGCAGCACAGGTGATG CTCATACAGTCCTGAGTGAGTCTAACAGAAATGATGAGCACTGTGGAAATTCTAACAACAAATGTGAAGT AATTCCAGAAAGCACCAGTGCAGTAACAAACATTCCACACGTGCAGATGGTTGCCATACAGGTATCTTGG AACATAATACACCAAGAAGTCAATACTGAGCCATATAATCCTTTTGAGGAACGACAAGGGGAAATTTCAC GGATTGAAAAGGAGCACCAAGTATTACAAGACCAACTTCAAGAAGTGTATGAAAATTATGAGCAGATAAA ACTTAAGGGCTTAGAAGAGACCAGGGACCTGGAAGAGAAGTTGAAAAGGCACTTAGAAGAAAACAAGATC TCAAAGACGGAATTAGATTGGTTCCTTCAAGATTTGGAAAGAGAAATTAAAAAATGGCAACAGGAAAAAA AAGAAATCCAAGAAAGACTAAAATCACTGAAGAAGAAAATTAAAAAGGTTTCAAATGCCAGTGAAATGTA TACCCAGAAAAATGATGGAAAGGAAAAGGAACATGAATTACATCTGGATCAGTCCCTTGAAATCAGCAAC ACACTTACAAATGAGAAAATGAAAATAGAAGAGTATATAAAGAAAGGGAAAGAGGATTATGAAGAGAGTC ATCAGAGAGCTGTGGCTGCAGAGGTATCCGTACTTGAAAACTGGAAGGAGAGTGAAGTGTATAAGCTACA GATCATGGAGTCACAAGCAGAAGCCTTTCTGAAGAAGCTGGGGCTGATTAGCCGTGATCCTGCAGCATAT CCTGACATGGAGTCTGATATACGTTCATGGGAATTGTTTCTTTCTAATGTTACAAAAGAAATTGAGAAAG CAAAGTCTCAGTTTGAAGAACAAATTAAGGCAATTAAAAATGGTTCTCGGCTCAGTGAACTTTCTAAAGT GCAGATTTCTGAGCTTTCATTTCCTGCCTGTAACACGGTTCATCCCGAGTTACTCCCTGAGTCTTCAGGC GACGATGGCCAAGGGCTTGTGACTTCTGCAAGCGACGTGACTGGAAACCACGCAGCACTTCACAGGGATC CTAGTGTGTTCTCTGCTGGTGATTCCCCAGGGGAGGCTCCTTCTGCGCTGTTGCCAGGGCCACCCCCTGG TCAGCCTGAAGCCACTCAGCTGACAGGGCCAAAACGGGCTGGCCAGGCAGCTCTGTCAGAACGAAGCCCT GTGGCTGATCGGAAGCAGCCTGTTCCTCCAGGACGTGCTGCGCGTTCAAGCCAGTCTCCAAAAAAGCCGT TCAATAGTATTATTGAGCACCTGTCAGTGGTATTCCCATGTTACAACAGCACTGAGCTTGCTGGTTTTAT TAAAAAAGTGCGAAGCAAAAACAAGAACTCACTCTCAGGATTGAGTATTGATGAAATTGTCCAAAGAGTG ACAGAACACATTCTAGATGAACAGAAAAAGAAAAAGCCAAACCCAGGAAAGGACAAGAGGACTTATGAGC CCAGCTCTGCCACCCCCGTGACCAGGTCCTCCCAGGGCTCACCCTCGGTGGTTGTTGCACCATCACCCAA AACCAAGGGGCAGAAAGCAGAAGATGTCCCTGTGAGGATTGCACTGGGTGCAAGTTCCTGTGAAATATGC CACGAGGTGTTCAAATCAAAAAACGTGCGTGTGCTCAAATGTGGGCACAAGTATCACAAAGGGTGCTTTA AGCAGTGGCTTAAAGGGCAGAGCGCTTGCCCGGCCTGCCAGGGTCGTGATCTCCTGACAGAAGAGTCACC TTCTGGAAGAGGCTGGCCCAGTCAGAATCAGGAGCTGCCTTCCTGCTCTTCTAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221541 representing NM_003316
Red=Cloning site Green=Tags(s) MDNFAEGDFTVADYALLEDCPHVDDCVFAAEFMSNDYVRVTQLYCDGVGVQYKDYIQSERNLEFDICSIW CSKPISVLQDYCDAIKINIFWPLLFQHQNSSVISRLHPCVDANNSRASEINLKKLQHLELMEDIVDLAKK VANDSFLIGGLLRIGCKIENKILAMEEALNWIKYAGDVTILTKLGSIDNCWPMLSIFFTEYKYHITKIVM EDCNLLEELKTQSCMDCIEEGELMKMKGNEEFSKERFDIAIIYYTRAIEYRPENYLLYGNRALCFLRTGQ FRNALGDGKRATILKNTWPKGHYRYCDALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQI EDLQGRTANKDPIKAFYENRAYTPRSLSAPIFTTSLNFVEKERDFRKINHEMANGGNQNLKVADEALKVD DCDCHPEFSPPSSQPPKHKGKQKSRNNESEKFSSSSPLTLPADLKNILEKQFSKSSRAAHQDFANIMKML RSLIQDGYMALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAEN QFKRIIEHYPSEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLIYRLPGVLTWPTSNVIIEESQPQKI KMLLEKFVEECKFPPVPDAICCYQKCHGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTF NDKIDKDFLQGICLTPDCEGVISKIIIFSSGGEVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKK LKRKIQKKEAKKLAQERMEEDLRESNPPKNEEQKETVDNVQRCQFLDDRILQCIKQYADKIKSGIQNTAM LLKELLSWKVLSTEDYTTCFSSRNFLNEAVDYVIRHLIQENNRVKTRIFLHVLSELKEVEPKLAAWIQKL NSFGLDATGTFFSRYGASLKLLDFSIMTFLWNEKYGHKLDSIEGKQLDYFSEPASLKEARCLIWLLEEHR DKFPALHSALDEFFDIMDSRCTVLRKQDSGEAPFSSTKVKNKSKKKKPKDSKPMLVGSGTTSVTSNNEII TSSEDHSNRNSDSAGPFAVPDHLRQDVEEFEALYDQHSNEYVVRNKKLWDMNPKQKCSTLYDYFSQFLEE HGPLDMSNKMFSAEYEFFPEETRQILEKAGGLKPFLLGCPRFVVIDNCIALKKVASRLKKKRKKKNIKTK VEEISKAGEYVRVKLQLNPAAREFKPDVKSKPVSDSSSAPAFENVKPKPVSANSPKPACEDVKAKPVSDN SSRQVSEDGQPKGVSSNSPKPGSEDANYKRVSCNSPKPVLEDVKPTYWAQSHLVTGYCTYLPFQRFDITQ TPPAYINVLPGLPQYTSIYTPLASLSPEYQLPRSVPVVPSFVANDRADKNAAAYFEGHHLNAENVAGHQI ASETQILEGSLGISVKSHCSTGDAHTVLSESNRNDEHCGNSNNKCEVIPESTSAVTNIPHVQMVAIQVSW NIIHQEVNTEPYNPFEERQGEISRIEKEHQVLQDQLQEVYENYEQIKLKGLEETRDLEEKLKRHLEENKI SKTELDWFLQDLEREIKKWQQEKKEIQERLKSLKKKIKKVSNASEMYTQKNDGKEKEHELHLDQSLEISN TLTNEKMKIEEYIKKGKEDYEESHQRAVAAEVSVLENWKESEVYKLQIMESQAEAFLKKLGLISRDPAAY PDMESDIRSWELFLSNVTKEIEKAKSQFEEQIKAIKNGSRLSELSKVQISELSFPACNTVHPELLPESSG DDGQGLVTSASDVTGNHAALHRDPSVFSAGDSPGEAPSALLPGPPPGQPEATQLTGPKRAGQAALSERSP VADRKQPVPPGRAARSSQSPKKPFNSIIEHLSVVFPCYNSTELAGFIKKVRSKNKNSLSGLSIDEIVQRV TEHILDEQKKKKPNPGKDKRTYEPSSATPVTRSSQGSPSVVVAPSPKTKGQKAEDVPVRIALGASSCEIC HEVFKSKNVRVLKCGHKYHKGCFKQWLKGQSACPACQGRDLLTEESPSGRGWPSQNQELPSCSSR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003316 |
| ORF Size | 6075 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003316.4 |
| RefSeq Size | 9021 bp |
| RefSeq ORF | 6078 bp |
| Locus ID | 7267 |
| UniProt ID | P53804 |
| Domains | TPR, RING |
| Protein Families | Druggable Genome |
| Gene Summary | E3 ubiquitin-protein ligase that mediates the ubiquitination and subsequent degradation of phosphorylated Akt (AKT1, AKT2 and AKT3) in the nucleus. Acts as a terminal regulator of Akt signaling after activation; its phosphorylation by Akt, which is a prerequisite for ubiquitin ligase activity, suggests the existence of a regulation mechanism required to control Akt levels after activation. Catalyzes the formation of 'Lys-48'-polyubiquitin chains. May play a role in neuronal differentiation inhibition via its interaction with CIT.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221541 | TTC3 (Myc-DDK-tagged)-Human tetratricopeptide repeat domain 3 (TTC3), transcript variant 1 |
CNY 20,048.00 |
|
| RC221541L1 | Lenti ORF clone of Human tetratricopeptide repeat domain 3 (TTC3), transcript variant 1, Myc-DDK-tagged |
CNY 22,448.00 |
|
| SC315382 | TTC3 (untagged)-Human tetratricopeptide repeat domain 3 (TTC3), transcript variant 1 |
CNY 26,790.00 |
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