United States
Germany
Japan
United Kingdom
China
NGLY1 (NM_001145294) Human Tagged ORF Clone
CAT＃: RG227428
- TrueORF®
NGLY1 (tGFP-tagged) - Human N-glycanase 1 (NGLY1), transcript variant 3
ORF Plasmid: DDK
"NM_001145294" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,990.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CDDG; CDG1V; PNG-1; PNG1; PNGase |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG227428 representing NM_001145294
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAAACCCTAATGATGAAAAATATAGATCCATCCGGATTGGAAACACAGCCTTTTCTACTAGACTCT TGCCTGTCAGAGGAGCTGTTGAATGTTTATTTGAAATGGGCTTTGAAGAGGGAGAAACACATCTCATCTT TCCTAAAAAAGCTTCAGTGGAGCAGCTGCAAAAAATTCGTGACCTGATTGCCATAGAGAGAAGTAGCAGA CTGGATGGCTCAAATAAGAGCCACAAAGTAAAGTCATCTCAGCAACCTGCAGCCAGTACCCAGCTTCCTA CAACACCATCTTCAAATCCCAGTGGGTTAAACCAGCACACAAGGAACCGTCAAGGGCAGTCATCAGATCC ACCATCTGCTTCAACGGTTGCTGCTGACTCAGCCATTCTAGAAGTTCTTCAGTCCAACATTCAGCATGTG CTGGTCTATGAAAATCCTGCTCTTCAGGAGAAAGCGTTGGCTTGTATTCCGGTCCAAGAACTAAAAAGGA AATCACAAGAAAAGTTATCGAGAGCTAGAAAATTGGATAAAGGTATCAATATAAGTGATGAGGATTTTCT TTTGCTGGAGCTTTTGCACTGGTTTAAGGAAGAATTTTTTCACTGGGTGAATAACGTTTTGTGCAGCAAA TGTGGTGGACAGACTAGGTCTAGAGATAGATCATTACTGCCCAGTGATGATGAGCTGAAGTGGGGTGCAA AGGAAGTGGAAGATCATTACTGTGATGCCTGCCAGTTCAGCAATCGATTCCCAAGATATAATAACCCTGA GAAACTTTTGGAAACAAGATGTGGACGGTGTGGCGAGTGGGCCAATTGTTTTACACTGTGCTGCCGAGCT GTAGGGTTTGAAGCTCGCTATGTTTGGGATTACACAGACCATGTCTGGACAGAAGTCTATTCTCCTTCTC AGCAGCGGTGGCTGCACTGTGATGCATGTGAAGATGTCTGTGACAAGCCACTCCTTTATGAAATAGGATG GGGCAAGAAGCTTTCCTATGTCATAGCATTTTCAAAAGATGAGGTAGTTGATGTCACTTGGCGATATTCC TGCAAACATGAAGAGGTGATTGCCAGAAGAACTAAGGTTAAAGAAGCATTACTTCGAGACACTATTAATG GGCTTAATAAGCAGAGGCAACTGTTTTTGTCAGAAAACAGAAGGAAAGAACTTCTCCAGAGGATAATTGT GGAGCTTGTTGAATTTATATCTCCCAAAACCCCTAAACCTGGAGAACTTGGGGGAAGAATATCTGGGTCA GTGGCTTGGAGAGTAGCCCGAGGTGAAATGGGTCTACAGAGAAAAGAAACCTTGTTTATTCCCTGTGAAA ATGAGAAGATTTCTAAACAGCTCCACCTTTGTTACAATATTGTGAAAGATCGTTATGTTCGAGTTTCAAA TAACAATCAAACCATTTCTGGATGGGAGAATGGCGTGTGGAAAATGGAATCTATATTCAGAAAAGTTGAA ACAGACTGGCACATGGTATATTTGGCCCGAAAGGAAGGATCATCTTTTGCTTATATTTCCTGGAAGTTTG AGTGTGGGTCAGTTGGCCTAAAAGTAGATAGCATTTCTATTAGAACAAGTAGTCAAACTTTTCAGACTGG AACAGTAGAATGGAAATTGCGATCTGATACAGCACAAGTAGAACTGACAGGCGATAACAGTCTTCACTCC TATGCTGATTTTTCTGGTGCCACTGAAGTTATTTTGGAAGCAGAATTAAGCAGAGGAGATGGTGATGTCG CTTGGCAACACACCCAGCTGTTTAGACAAAGCTTAAATGACCATGAAGAAAATTGTTTGGAGATAATTAT AAAATTCAGTGACCTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG227428 representing NM_001145294
Red=Cloning site Green=Tags(s) MRNPNDEKYRSIRIGNTAFSTRLLPVRGAVECLFEMGFEEGETHLIFPKKASVEQLQKIRDLIAIERSSR LDGSNKSHKVKSSQQPAASTQLPTTPSSNPSGLNQHTRNRQGQSSDPPSASTVAADSAILEVLQSNIQHV LVYENPALQEKALACIPVQELKRKSQEKLSRARKLDKGINISDEDFLLLELLHWFKEEFFHWVNNVLCSK CGGQTRSRDRSLLPSDDELKWGAKEVEDHYCDACQFSNRFPRYNNPEKLLETRCGRCGEWANCFTLCCRA VGFEARYVWDYTDHVWTEVYSPSQQRWLHCDACEDVCDKPLLYEIGWGKKLSYVIAFSKDEVVDVTWRYS CKHEEVIARRTKVKEALLRDTINGLNKQRQLFLSENRRKELLQRIIVELVEFISPKTPKPGELGGRISGS VAWRVARGEMGLQRKETLFIPCENEKISKQLHLCYNIVKDRYVRVSNNNQTISGWENGVWKMESIFRKVE TDWHMVYLARKEGSSFAYISWKFECGSVGLKVDSISIRTSSQTFQTGTVEWKLRSDTAQVELTGDNSLHS YADFSGATEVILEAELSRGDGDVAWQHTQLFRQSLNDHEENCLEIIIKFSDL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001145294 |
| ORF Size | 1836 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001145294.1, NP_001138766.1 |
| RefSeq Size | 2539 bp |
| RefSeq ORF | 1839 bp |
| Locus ID | 55768 |
| UniProt ID | Q96IV0 |
| Gene Summary | This gene encodes an enzyme that catalyzes hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue to N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. The encoded enzyme may play a role in the proteasome-mediated degradation of misfolded glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227428 | NGLY1 (Myc-DDK-tagged)-Human N-glycanase 1 (NGLY1), transcript variant 3 |
CNY 4,560.00 |
|
| RC227428L3 | Lenti-ORF clone of NGLY1 (Myc-DDK-tagged)-Human N-glycanase 1 (NGLY1), transcript variant 3 |
CNY 7,320.00 |
|
| RC227428L4 | Lenti-ORF clone of NGLY1 (mGFP-tagged)-Human N-glycanase 1 (NGLY1), transcript variant 3 |
CNY 7,320.00 |
|
| SC326108 | NGLY1 (untagged)-Human N-glycanase 1 (NGLY1), transcript variant 3 |
CNY 9,880.00 |
