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PLK4 (NM_001190799) Human Tagged ORF Clone
CAT＃: RG231445
- TrueORF®
PLK4 (tGFP-tagged) - Human polo-like kinase 4 (PLK4), transcript variant 2
ORF Plasmid: DDK
"NM_001190799" in other vectors (3)
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CNY 12,592.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MCCRP2; SAK; STK18 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG231445 representing NM_001190799
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACCTGCATCGGGGAGAAGATCGAGGATTTTAAAGTTGGAAATCTGCTTGGTAAAGGATCATTTG CTGGTGTCTACAGAGCTGAGTCCATTCACACTGGTTTGGAAGTTGCAATCAAAATGCTTTATAACTATTT TGAAGATAGCAATTATGTGTATCTGGTATTAGAAATGTGCCATAATGGAGAAATGAACAGGTATCTAAAG AATAGAGTGAAACCCTTCTCAGAAAATGAAGCTCGACACTTCATGCACCAGATCATCACAGGGATGTTGT ATCTTCATTCTCATGGTATACTACACCGGGACCTCACACTTTCTAACCTCCTACTGACTCGTAATATGAA CATCAAGATTGCTGATTTTGGGCTGGCAACTCAACTGAAAATGCCACATGAAAAGCACTATACATTATGT GGAACTCCTAACTACATTTCACCAGAAATTGCCACTCGAAGTGCACATGGCCTTGAATCTGATGTTTGGT CCCTGGGCTGTATGTTTTATACATTACTTATCGGGAGACCACCCTTCGACACTGACACAGTCAAGAACAC ATTAAATAAAGTAGTATTGGCAGATTATGAAATGCCATCTTTTTTGTCAATAGAGGCCAAGGACCTTATT CACCAGTTACTTCGTAGAAATCCAGCAGATCGTTTAAGTCTGTCTTCAGTATTGGACCATCCTTTTATGT CCCGAAATTCTTCAACAAAAAGTAAAGATTTAGGAACTGTGGAAGACTCAATTGATAGTGGGCATGCCAC AATTTCTACTGCAATTACAGCTTCTTCCAGTACCAGTATAAGTGGTAGTTTATTTGACAAAAGAAGACTT TTGATTGGTCAGCCACTCCCAAATAAAATGACTGTATTTCCAAAGAATAAAAGTTCAACTGATTTTTCTT CTTCAGGAGATGGAAACAGTTTTTATACTCAGTGGGGAAATCAAGAAACCAGTAATAGTGGAAGGGGAAG AGTAATTCAAGATGCAGAAGAAAGGCCACATTCTCGATACCTTCGTAGAGCTTATTCCTCTGATAGATCT GGCACTTCTAATAGTCAGTCTCAAGCAAAAACATATACAATGGAACGATGTCACTCAGCAGAAATGCTTT CAGTGTCCAAAAGATCAGGAGGAGGTGAAAATGAAGAGAGGTACTCACCCACAGACAACAATGCCAACAT TTTTAACTTCTTTAAAGAAAAGACATCCAGTAGTTCTGGATCTTTTGAAAGACCTGATAACAATCAAGCA CTCTCCAATCATCTTTGTCCAGGAAAAACTCCTTTTCCATTTGCAGACCCGACACCTCAGACTGAAACCG TACAACAGTGGTTTGGGAATCTGCAAATAAATGCTCATTTAAGAAAAACTACTGAATATGACAGCATCAG CCCAAACCGGGACTTCCAGGGCCATCCAGATTTGCAGAAGGACACATCAAAAAATGCCTGGACTGATACA AAAGTCAAAAAGAACTCTGATGCTTCTGATAATGCACATTCTGTAAAACAGCAAAATACCATGAAATATA TGACTGCACTTCACAGTAAACCTGAGATAATCCAACAAGAATGTGTTTTTGGCTCAGATCCTCTTTCTGA ACAGAGCAAGACTAGGGGTATGGAGCCACCATGGGGTTATCAGAATCGTACATTAAGAAGCATTACATCT CCGTTGGTTGCTCACAGGTTAAAACCAATCAGACAGAAAACCAAAAAGGCTGTGGTGAGCATACTTGATT CAGAGGAGGTGTGTGTGGAGCTTGTAAAGGAGTATGCATCTCAAGAATATGTGAAAGAAGTTCTTCAGAT ATCTAGTGATGGAAATACGATCACTATTTATTATCCAAATGGTGGTAGAGGTTTTCCTCTTGCTGATAGA CCACCCTCACCTACTGACAACATCAGTAGGTACAGCTTTGACAATTTACCAGAAAAATACTGGCGAAAAT ATCAATATGCTTCCAGGTTTGTACAGCTTGTAAGATCTAAATCTCCCAAAATCACTTATTTTACAAGATA TGCTAAATGCATTTTGATGGAGAATTCTCCTGGTGCTGATTTTGAGGTTTGGTTTTATGATGGGGTAAAA ATACACAAAACAGAAGATTTCATTCAGGTGATTGAAAAGACAGGGAAGTCTTACACTTTAAAAAGTGAAA GTGAAGTTAATAGCTTGAAAGAGGAGATAAAAATGTATATGGACCATGCTAATGAGGGTCATCGTATTTG TTTAGCACTGGAATCCATAATTTCAGAAGAGGAAAGGAAAACTAGGAGTGCTCCCTTTTTCCCAATAATC ATAGGAAGAAAACCTGGTAGTACTAGTTCACCTAAGGCCTTATCACCTCCTCCTTCTGTGGATTCAAATT ACCCAACGAGAGAGAGAGCATCTTTCAACAGAATGGTCATGCATAGTGCTGCTTCTCCAACACAGGCACC AATCCTTAATCCCTCTATGGTTACAAATGAAGGACTTGGTCTTACAACTACAGCTTCTGGAACAGACATC TCTTCTAATAGTCTAAAAGATTGTCTTCCTAAATCAGCACAACTTTTGAAATCTGTTTTTGTGAAAAATG TTGGTTGGGCTACACAGTTAACTAGTGGAGCTGTGTGGGTTCAGTTTAATGATGGGTCCCAGTTGGTTGT GCAGGCAGGAGTGTCTTCTATCAGTTATACCTCACCAAATGGTCAAACAACTAGGTATGGAGAAAATGAA AAATTACCAGACTACATCAAACAGAAATTACAGTGTCTGTCTTCCATCCTTTTGATGTTTTCTAATCCGA CTCCTAATTTTCAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG231445 representing NM_001190799
Red=Cloning site Green=Tags(s) MATCIGEKIEDFKVGNLLGKGSFAGVYRAESIHTGLEVAIKMLYNYFEDSNYVYLVLEMCHNGEMNRYLK NRVKPFSENEARHFMHQIITGMLYLHSHGILHRDLTLSNLLLTRNMNIKIADFGLATQLKMPHEKHYTLC GTPNYISPEIATRSAHGLESDVWSLGCMFYTLLIGRPPFDTDTVKNTLNKVVLADYEMPSFLSIEAKDLI HQLLRRNPADRLSLSSVLDHPFMSRNSSTKSKDLGTVEDSIDSGHATISTAITASSSTSISGSLFDKRRL LIGQPLPNKMTVFPKNKSSTDFSSSGDGNSFYTQWGNQETSNSGRGRVIQDAEERPHSRYLRRAYSSDRS GTSNSQSQAKTYTMERCHSAEMLSVSKRSGGGENEERYSPTDNNANIFNFFKEKTSSSSGSFERPDNNQA LSNHLCPGKTPFPFADPTPQTETVQQWFGNLQINAHLRKTTEYDSISPNRDFQGHPDLQKDTSKNAWTDT KVKKNSDASDNAHSVKQQNTMKYMTALHSKPEIIQQECVFGSDPLSEQSKTRGMEPPWGYQNRTLRSITS PLVAHRLKPIRQKTKKAVVSILDSEEVCVELVKEYASQEYVKEVLQISSDGNTITIYYPNGGRGFPLADR PPSPTDNISRYSFDNLPEKYWRKYQYASRFVQLVRSKSPKITYFTRYAKCILMENSPGADFEVWFYDGVK IHKTEDFIQVIEKTGKSYTLKSESEVNSLKEEIKMYMDHANEGHRICLALESIISEEERKTRSAPFFPII IGRKPGSTSSPKALSPPPSVDSNYPTRERASFNRMVMHSAASPTQAPILNPSMVTNEGLGLTTTASGTDI SSNSLKDCLPKSAQLLKSVFVKNVGWATQLTSGAVWVQFNDGSQLVVQAGVSSISYTSPNGQTTRYGENE KLPDYIKQKLQCLSSILLMFSNPTPNFH TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001190799 |
| ORF Size | 2814 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001190799.2 |
| RefSeq Size | 3774 bp |
| RefSeq ORF | 2817 bp |
| Locus ID | 10733 |
| UniProt ID | O00444 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. Three alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC231445 | PLK4 (Myc-DDK-tagged)-Human polo-like kinase 4 (PLK4), transcript variant 2 |
CNY 9,790.00 |
|
| RC231445L3 | Lenti-ORF clone of PLK4 (Myc-DDK-tagged)-Human polo-like kinase 4 (PLK4), transcript variant 2 |
CNY 11,690.00 |
|
| RC231445L4 | Lenti-ORF clone of PLK4 (mGFP-tagged)-Human polo-like kinase 4 (PLK4), transcript variant 2 |
CNY 11,690.00 |
