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ZNF331 (NM_001253798) Human Tagged ORF Clone
CAT＃: RG234174
- TrueORF®
ZNF331 (tGFP-tagged) - Homo sapiens zinc finger protein 331 (ZNF331), transcript variant 3
ORF Plasmid: DDK
"NM_001253798" in other vectors (2)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | RITA; ZNF361; ZNF463 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234174 representing NM_001253798
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGGGTTTGGTGACGTTCGCCGACGTAGCCATAGACTTTTCTCAGGAGGAGTGGGCCTGTCTGA ACTCTGCTCAGAGGGACCTGTACTGGGACGTGATGCTGGAGAACTACAGTAACTTGGTCTCACTGGATTT GGAGTCAGCATATGAAAATAAGAGTTTACCTACAGAAAAAAACATTCATGAAATAAGGGCTTCCAAAAGG AATTCAGATAGAAGAAGTAAATCCCTTGGCCGTAACTGGATATGTGAAGGTACGCTTGAAAGACCACAGC GCTCCAGAGGGAGGTATGTCAATCAGATGATCATCAATTATGTCAAAAGACCTGCTACTAGAGAAGGCAC CCCTCCTAGAACACATCAGAGACATCATAAGGAGAATTCCTTTGAATGTAAGGACTGTGGGAAGGCCTTT AGTCGTGGCTATCAACTTAGTCAACATCAGAAAATCCATACTGGTGAGAAACCTTATGAATGTAAAGAAT GTAAGAAGGCCTTCCGTTGGGGCAATCAGCTTACTCAACATCAAAAAATTCATACTGGGGAGAAGCCCTA CGAATGTAAAGACTGTGGGAAGGCTTTTCGATGGGGCTCAAGCCTCGTTATTCATAAGAGGATTCATACT GGTGAAAAACCCTATGAATGTAAAGACTGTGGAAAGGCCTTTCGGCGTGGTGATGAGCTCACTCAGCACC AGAGATTCCACACTGGGGAGAAAGACTACGAATGCAAAGACTGTGGGAAGACCTTTAGCCGTGTGTATAA ACTTATTCAGCACAAGAGAATTCATAGTGGGGAGAAGCCTTACGAGTGTAAAGACTGTGGGAAGGCTTTT ATTTGTGGTTCAAGCCTCATTCAGCATAAAAGAATTCACACAGGTGAGAAACCCTATGAATGTCAAGAAT GTGGGAAGGCCTTTACTCGAGTCAATTACCTTACTCAGCATCAGAAGATCCACACCGGTGAGAAGCCTCA CGAATGTAAGGAGTGTGGGAAGGCCTTTCGCTGGGGTTCGAGCCTCGTTAAGCACGAGAGGATACATACG GGCGAGAAGCCGTACAAGTGCACAGAATGTGGGAAGGCCTTCAATTGTGGCTATCACCTCACTCAGCACG AGAGAATCCACACAGGCGAAACCCCGTATAAATGTAAGGAGTGTGGGAAGGCTTTCATTTATGGATCGAG CCTCGTGAAACATGAGAGAATTCATACCGGGGTGAAACCCTATGGGTGTACAGAATGTGGGAAGAGCTTT AGTCACGGCCATCAGCTTACACAACATCAGAAAACGCACAGTGGGGCGAAATCCTACGAATGTAAGGAGT GCGGGAAGGCATGTAACCACCTAAACCATCTCCGAGAACATCAGAGGATCCACAACAGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234174 representing NM_001253798
Red=Cloning site Green=Tags(s) MAQGLVTFADVAIDFSQEEWACLNSAQRDLYWDVMLENYSNLVSLDLESAYENKSLPTEKNIHEIRASKR NSDRRSKSLGRNWICEGTLERPQRSRGRYVNQMIINYVKRPATREGTPPRTHQRHHKENSFECKDCGKAF SRGYQLSQHQKIHTGEKPYECKECKKAFRWGNQLTQHQKIHTGEKPYECKDCGKAFRWGSSLVIHKRIHT GEKPYECKDCGKAFRRGDELTQHQRFHTGEKDYECKDCGKTFSRVYKLIQHKRIHSGEKPYECKDCGKAF ICGSSLIQHKRIHTGEKPYECQECGKAFTRVNYLTQHQKIHTGEKPHECKECGKAFRWGSSLVKHERIHT GEKPYKCTECGKAFNCGYHLTQHERIHTGETPYKCKECGKAFIYGSSLVKHERIHTGVKPYGCTECGKSF SHGHQLTQHQKTHSGAKSYECKECGKACNHLNHLREHQRIHNS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001253798 |
| ORF Size | 1389 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001253798.2 |
| RefSeq Size | 4167 bp |
| RefSeq ORF | 1392 bp |
| Locus ID | 55422 |
| UniProt ID | Q9NQX6 |
| Protein Families | Transcription Factors |
| Gene Summary | This gene encodes a zinc finger protein containing a KRAB (Kruppel-associated box) domain found in transcriptional repressors. This gene may be methylated and silenced in cancer cells. This gene is located within a differentially methylated region (DMR) and shows allele-specific expression in placenta. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding the same protein. [provided by RefSeq, Nov 2015] |
Documents
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