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NOLC1 (NM_001284388) Human Tagged ORF Clone
CAT＃: RG239378
- TrueORF®
NOLC1 (tGFP-tagged) - Human nucleolar and coiled-body phosphoprotein 1 (NOLC1), transcript variant 1
ORF Plasmid: DDK
"NM_001284388" in other vectors (2)
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CNY 6,180.00
货期*
2周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | NOPP130; NOPP140; NS5ATP13; P130; Srp40 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG239378 representing NM_001284388.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGACGCCGGCATTCGCCGCGTGGTTCCCAGCGACCTGTATCCCCTCGTGCTCGGCTTCCTGCGC GATAACCAACTCTCAGAGGTGGCCAATAAGTTCGCCAAAGCGACAGGAGCTACACAGCAGGATGCCAAT GCCTCTTCCCTCTTAGACATCTATAGCTTCTGGCTCAAGTCTGCCAAGGTCCCAGAGCGAAAGTTACAG GCAAATGGACCAGTGGCTAAGAAAGCTAAGAAGAAGGCCTCATCCAGTGACAGTGAGGACAGCAGCGAG GAGGAGGAGGAAGTTCAAGGGCCTCCAGCAAAGAAGGCTGCTGTACCTGCCAAGCGAGTCGGTCTGCCT CCTGGGAAGGCTGCAGCCAAAGCATCAGAGAGTAGCAGCAGTGAAGAGTCCAGTGATGATGATGATGAG GAGGACCAAAAGAAACAGCCTGTCCAGAAGGGAGTTAAGCCCCAAGCCAAGGCAGCCAAAGCTCCTCCT AAGAAGGCCAAGAGCTCTGATTCTGATTCTGACTCAAGCTCCGAGGATGAGCCACCAAAGAACCAGAAG CCAAAGATAACACCTGTGACAGTTAAAGCTCAGACTAAAGCCCCTCCCAAACCAGCTCGAGCAGCACCT AAAATAGCCAATGGTAAAGCAGCCAGTAGCAGCAGTAGCAGCAGCAGCAGCAGTAGCAGTGATGACTCA GAGGAGGAGAAGGCAGCAGCCACCCCCAAGAAGGTCTGGACCATAACTTCTGTCAGGGCAGAGACTGTA CCTAAAAAGCAAGTTGTGGCCAAGGCCCCAGTGAAAGCAGCTACCACCCCTACCCGGAAGAGTTCTAGC AGTGAGGATTCCTCCAGTGACGAGGAAGAGGAGCAAAAAAAACCCATGAAAAATAAACCAGGTCCCTAC AGTTCAGTCCCCCCGCCTTCTGCTCCCCCACCAAAGAAGTCTCTGGGAACCCAGCCTCCCAAGAAGGCT GTGGAGAAGCAGCAGCCTGTGGAAAGCAGTGAAGACAGCAGTGATGAGTCTGATTCAAGTTCTGAAGAA GAGAAGAAACCCCCAACTAAGGCAGTAGTCTCTAAAGCAACCACTAAACCACCTCCAGCAAAGAAAGCA GCAGAGAGCTCTTCAGACAGCTCAGACTCTGACAGCTCTGAGGATGATGAAGCTCCTTCTAAGCCAGCT GGTACCACCAAGAATTCTTCAAATAAGCCAGCTGTCACCACCAAGTCACCTGCAGTGAAGCCAGCTGCA GCCCCCAAGCAACCTGTGGGCGGTGGCCAGAAGCTTCTGACGAGAAAGGCTGACAGCAGCTCCAGTGAG GAAGAGAGCAGCTCCAGTGAGGAGGAGAAGACAAAGAAGATGGTGGCCACCACTAAGCCCAAGGCGACT GCCAAAGCAGCTCTATCTCTGCCTGCCAAGCAGGCTCCTCAGGGTAGTAGGGACAGCAGCTCTGATTCA GACAGCTCCAGCAGTGAGGAGGAGGAAGAGAAGACATCTAAGTCTGCAGTTAAGAAGAAGCCACAGAAG GTAGCAGGAGGTGCAGCCCCTTCCAAGCCAGCCTCTGCAAAGAAAGGAAAGGCTGAGAGCAGCAACAGT TCTTCTTCTGATGACTCCAGTGAGGAAGAGGAAGAGAAGCTCAAGGGCAAGGGCTCTCCAAGACCACAA GCCCCCAAGGCCAATGGCACCTCTGCACTGACTGCCCAGAATGGAAAAGCAGCTAAGAACAGTGAGGAG GAGGAAGAAGAAAAGAAAAAGGCGGCAGTGGTAGTTTCCAAATCAGGTTCATTAAAGAAGCGGAAGCAG AATGAGGCTGCCAAGGAGGCAGAGACTCCTCAGGCCAAGAAGATAAAGCTTCAGACCCCTAACACATTT CCAAAAAGGAAGAAAGGAGAAAAAAGGGCATCATCCCCATTCCGAAGGGTCAGGGAGGAGGAAATTGAG GTGGATTCACGAGTTGCGGACAACTCCTTTGATGCCAAGCGAGGTGCAGCCGGAGACTGGGGAGAGCGA GCCAATCAGGTTTTGAAGTTCACCAAAGGCAAGTCCTTTCGGCATGAGAAAACCAAGAAGAAGCGGGGC AGCTACCGGGGAGGCTCAATCTCTGTCCAGGTCAATTCTATTAAGTTTGACAGCGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG239378
Blue=ORF Red=Cloning site Green=Tag(s) MADAGIRRVVPSDLYPLVLGFLRDNQLSEVANKFAKATGATQQDANASSLLDIYSFWLKSAKVPERKLQ ANGPVAKKAKKKASSSDSEDSSEEEEEVQGPPAKKAAVPAKRVGLPPGKAAAKASESSSSEESSDDDDE EDQKKQPVQKGVKPQAKAAKAPPKKAKSSDSDSDSSSEDEPPKNQKPKITPVTVKAQTKAPPKPARAAP KIANGKAASSSSSSSSSSSSDDSEEEKAAATPKKVWTITSVRAETVPKKQVVAKAPVKAATTPTRKSSS SEDSSSDEEEEQKKPMKNKPGPYSSVPPPSAPPPKKSLGTQPPKKAVEKQQPVESSEDSSDESDSSSEE EKKPPTKAVVSKATTKPPPAKKAAESSSDSSDSDSSEDDEAPSKPAGTTKNSSNKPAVTTKSPAVKPAA APKQPVGGGQKLLTRKADSSSSEEESSSSEEEKTKKMVATTKPKATAKAALSLPAKQAPQGSRDSSSDS DSSSSEEEEEKTSKSAVKKKPQKVAGGAAPSKPASAKKGKAESSNSSSSDDSSEEEEEKLKGKGSPRPQ APKANGTSALTAQNGKAAKNSEEEEEEKKKAAVVVSKSGSLKKRKQNEAAKEAETPQAKKIKLQTPNTF PKRKKGEKRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRG SYRGGSISVQVNSIKFDSE TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001284388 |
| ORF Size | 2127 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001284388.1, NP_001271317.1 |
| RefSeq Size | 3977 bp |
| RefSeq ORF | 2130 bp |
| Locus ID | 9221 |
| UniProt ID | Q14978 |
| Protein Families | Stem cell - Pluripotency |
| MW | 75.2 kDa |
| Gene Summary | Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (PubMed:10567578, PubMed:26399832). Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed:26399832). Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus (PubMed:9016786). It has intrinsic GTPase and ATPase activities (PubMed:9016786).[UniProtKB/Swiss-Prot Function] |
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