Dctd (NM_001161512) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN215954 representing NM_001161512 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTTATACACCCAACATGAGTGACATTTCCTGCAAAAAACGGGATGACTATCTGGAGTGGCCGGAGT ATTTCATGGCGGTAGCCTTCTTGTCAGCACAAAGAAGCAAGGACCCAAGTTCCCAGGTGGGAGCCTGCAT TGTGAATACAGAAAACAAGATCGTTGGGATCGGGTACAATGGGATGCCAAATGGGTGCAGCGATGACCTG TTGCCCTGGAGGAGAACAGCAGAAAACAAGCTGGATACTAAATACCCTTATGTGTGCCACGCCGAGCTGA ACGCCATCATGAACAAGAATTCGGCCGACGTGAAGGGCTGCAGTATGTATGTGGCCTTGTTCCCATGTAA TGAATGTGCGAAGCTCATCATTCAGGCAGAAAATTCACACCAAAGTACAGCAAGATTGTCATTGATTTCG ATTCGATTAATAGCCGTCCGAGTCAGAAGCCTCAGTGAGTTTCGAGCCCCGGAAGAAGATGACACCCCAC ACACAAGGGGCTCCCTGAGCCTTTCCTCTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001161512
Insert Size	522 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001161512.1, NP_001154984.1
RefSeq Size	2021 bp
RefSeq ORF	522 bp
Locus ID	290741
UniProt ID	Q5M9G0
Gene Summary	Supplies the nucleotide substrate for thymidylate synthetase.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, and lacks an alternate exon in the 3' coding region that causes a frameshift, compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1.

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