Prkcb (NM_001172305) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Pkcb; Prkcb1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217086 representing NM_001172305 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGACCCGGCTGCGGGGCCGCCGCCGAGCGAGGGCGAGGAGAGCACGGTGCGCTTCGCCCGCAAAG GCGCCCTCCGGCAGAAGAACGTGCACGAGGTGAAGAACCACAAATTCACCGCCCGCTTCTTCAAGCAGCC CACCTTCTGCAGCCACTGCACCGACTTCATTTGGGGCTTAGGTTTGCAGGGATTCCAGAGTCAGGTCTGC TGCTTTGTTGTACACAAGCGCTGCCATGAATTCGTCACGTTCTCCTGCCCTGGTGCAGACAAGGGCCCGG CCTCTGATGACCCACGGAGCAAACACAAGTTTAAGATCCACACCTACTCCAGCCCTACCTTCTGTGACCA CTGTGGATCACTGCTGTATGGGCTCATCCACCAGGGGATGAAATGCGACACCTGTATGATGAATGTCCAC AAGCGCTGCGTGATGAACGTCCCCAGCCTCTGTGGCACCGACCACACAGAACGCCGTGGCCGCATCTACA TCCAGGCCCACATCGACAGGGAGGTCCTCATCGTTGTTGTAAGAGATGCTAAAAATCTGGTACCTATGGA CCCCAACGGCTTGTCAGATCCCTACGTAAAACTGAAACTGATCCCTGATCCCAAAAGTGAGAGCAAGCAG AAGACCAAGACTATCAAATGCTCCCTCAACCCGGAGTGGAACGAAACCTTCAGATTTCAGCTGAAGGAAT CAGACAAAGACAGAAGACTGTCCGTAGAGATCTGGGATTGGGACCTGACCAGCAGGAATGACTTCATGGG ATCTCTGTCGTTTGGGATTTCAGAACTACAGAAAGCCGGAGTGGATGGCTGGTTCAAGTTACTAAGCCAG GAAGAAGGCGAGTACTTTAATGTGCCGGTGCCGCCGGAAGGAAGCGAGGGCAATGAAGAGCTGCGGCAGA AGTTTGAGAGAGCCAAGATTGGCCAAGGTACCAAGGCTCCAGAAGAAAAGACAGCGAACACTATATCCAA ATTTGACAACAATGGCAACAGGGACCGGATGAAACTGACCGATTTTAACTTCCTGATGGTGCTGGGGAAA GGCAGCTTTGGCAAGGTCATGCTCTCAGAGCGGAAGGGTACAGATGAACTCTATGCCGTGAAGATCCTGA AGAAAGATGTGGTGATCCAAGATGACGATGTGGAGTGCACAATGGTGGAGAAGAGGGTGCTGGCCCTGCC TGGGAAGCCCCCATTCCTGACTCAGCTCCATTCCTGCTTCCAGACCATGGACCGCCTCTACTTTGTGATG GAGTATGTGAACGGGGGCGACCTCATGTACCACATCCAACAAGTTGGCCGTTTCAAGGAGCCCCATGCTG TATTTTACGCTGCAGAGATTGCCATCGGTCTTTTCTTCTTGCAGAGCAAGGGCATCATTTACCGTGACCT GAAACTTGACAACGTGATGCTGGATTCCGAGGGGCACATCAAAATCGCTGACTTTGGCATGTGTAAAGAG AATATCTGGGATGGGGTGACAACCAAGACATTCTGTGGCACTCCAGACTACATTGCCCCAGAGATCATTG CTTATCAGCCCTACGGGAAGTCTGTGGACTGGTGGGCGTTTGGAGTCCTGCTGTATGAAATGTTGGCTGG CCAGGCACCTTTTGAAGGGGAGGATGAGGATGAACTCTTCCAGTCAATCATGGAGCACAACGTGGCGTAT CCCAAGTCCATGTCTAAGGAAGCTGTGGCAATCTGCAAAGGGCTAATGACCAAACACCCAGGCAAGCGCC TGGGTTGTGGGCCTGAAGGGGAACGAGACATTAAGGAGCATGCATTTTTCCGGTATATCGACTGGGAGAA ACTCGAACGCAAGGAGATTCAGCCACCTTATAAACCAAAAGCTAGAGACAAGCGAGACACCTCCAACTTC GACAAAGAGTTCACCAGGCAGCCTGTGGAACTGACTCCCACTGACAAACTCTTCATCATGAACTTGGACC AAAATGAATTTGCTGGCTTCTCGTATACTAACCCAGAGTTTGTCATTAATGTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001172305
Insert Size	2016 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001172305.1, NP_001165776.1
RefSeq Size	2666 bp
RefSeq ORF	2016 bp
Locus ID	25023
UniProt ID	P68403
Gene Summary	plays a role in hexosamine biosynthesis pathway induced transcriptional regulation [RGD, Feb 2006] Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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