Myo9b (NM_001271067) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217723 representing NM_001271067 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGCTCACGAGGCTGGCAGCTCAGGCCGAAGGCGGCCGGCCACCTTCCACCTGCACATCTACCCTC AGCTGCCCAGTGCTGGGAGCCAGACCTCATGCCGTGTAACCGCCACCAAGGACAGCACAACAAGCGATGT CATCCGAGACGTGGTGGCCAGTCTACACCTGGACGGCTCGAAGCACTATGTGCTGGTGGAGGTGAAGGAG TCGGGTGGGGAGGAGTGGGTGCTGGATGCCAGCGACTCGCCTGTGCACCGAGTGCTGCTGTGGCCTCGGC GAGCGCAGAAGGAGCACCCTCGGGAGGACGGCTACTACTTCCTGCTGCAGGAGCGCAACGCTGACGGCAG CATTCAGTACCTGCACGTGCAGCTGCTGGCTCAGCCCACAGCCGCATGTCGCCTGGTGGAGCGAGGGCTG CTGCCAAGGCCTCAAGCGGACTTCGACGACCTGTGCAACCTGCCGGAGCTGAACGAGGCCAACCTGCTGC AGAGCCTGAAGCTGCGCTTCGTGCAGCAGAAGATCTACACGTACGCGGGCAGCATCCTGGTGGCCATCAA CCCCTTCAAGTTCCTGCCCATTTACAACCCCAAGTATGTGAAGATGTACGAGAACCAGCAGCTGGGAAAG CTGGAGCCGCACGTGTTCGCTCTGGCCGATGTAGCCTACTACACCATGCTGCGCAAGCACGTGAACCAGT GCATCGTCATCTCTGGTGAGAGCGGGTCCGGCAAGACGCAAAGCACCAACTTCCTCATCCACTGCCTCAC AGCGCTCAGCCAGAAGGGCTACGCCAGCGGCGTCGAGAGGACCATCCTGGGTGCAGGGCCTGTGCTGGAG GCTTTTGGGAACGCCAAGACAGCCCACAACAACAACTCCAGCCGCTTCGGGAAGTTCATCCAAGTCAACT ACCTGGAGAGTGGCATCGTGAGGGGAGCTGTTGTGGAAAAATACCTTCTTGAAAAGTCTCGCCTGGTTTC CCAGGAGAAGGATGAGCGGAACTACCATGTGTTTTATTATCTGCTGCTGGGCGTCAGTGAGGAAGAGCGT CAGGAATTTCAGCTGAAGCAGCCTCAAGACTATTTCTACCTCAACCAGCATAACTTGAATATTGAAGATG GAGAAGACCTCAAACATGACTTTGAAAGGCTTCAGCAGGCCATGGAGATGGTGGGCTTCCTACCTGCCAC GAAGAAGCAGATCTTCTCTGTCCTCTCAGCCATCCTGTATCTTGGCAATGTCACCTATAAGAAGAGAGCC ACAGGCCGGGATGAAGGCCTGGAGGTCGGGCCCCCGGAGGTATTGGACACACTATCCCAGCTCCTGAAGG TAAAGCGGGAGACCCTGGTGGAGGTCCTAACCAAGAGAAAAACAATCACGGTCAATGACAAACTCATCCT GCCTTACAGCCTCAGTGAGGCTATCACTGCACGAGACTCCATGGCTAAGTCTCTGTACAGCGCCCTGTTT GACTGGATTGTGCTGAGGATCAACCACGCCCTCCTCAACAAGAAGGACATGGAAGAGGCTGTTTCTTGCT TGTCCATTGGCGTCCTGGACATATTCGGATTTGAGGACTTTGAACGGAACAGCTTCGAGCAGTTTTGCAT CAACTACGCCAACGAGCAGTTGCAGTACTACTTCACCCAGCACATCTTCAAGCTGGAGCAGGAGGAGTAC CAGGGTGAGGGTATCTCGTGGCACAACATTGACTACACCGACAACGTGGGCTGTATCCACCTCATCAGCA AGAAGCCCACTGGCCTCTTCTACCTGCTGGACGAGGAAAGCAACTTTCCCCATGCCACAAGCCACACTCT GCTGGCCAAATTCAAGCAGCAGCATGAGGACAACAAGTACTTCCTGGGCACACCAGTCCTGGAGCCCGCC TTCATCATCCAGCACTTCGCAGGCAGAGTAAAGTACCAGATCAAGGACTTCCGGGAGAAGAACATGGACT ACATGCGGCCTGACATCGTGGCACTGCTAAGGGGCAGTGACAGCTCCTATGTGCGCCAACTCATCGGCAT GGACCCGGTAGCTGTGTTCCGCTGGGCTGTATTACGGGCAGCCATCAGGGCCATGGCTGTGCTGCGGGAG GCTGGGCGCCTGCGTGCAGAGAGAGCAGAGAAAGCAGAAGCAGGTGTAAGTAGTCCTGTCACTCGAAGTC ACGTGGAAGAGCTGCCAAGAGGAGCCAACACCCCTTCAGAGAAACTGTACCGCTGCGCAGGGCTAGACTT CTCCTTTGAGCGCTCTGAGGAGCTGGATGTTAACGCTTTTGAGGACATCATGGCTTTCTATGAGAGCAGG AACGATTTGCATAACCAAATCATCAAGAGCCTCAAAGGACTGCCATGGCAGGGCGAGGACCCGCGGAGGC TTCTCCAGTCCCTCAGTCGGCTCCAGAAGCCCCGCACCTTCTTCCTGAAGAGTAAAGGTATCAAACAAAA GCAGATCATTCCCAAGAACCTGCTGGACTCTAAGTCCCTGAGGCTCATCATCAGCATGACGCTGCATGAC CGAACTACCAAGTCACTGCTGCACCTGCACAAGAAGAAGAAGCCGCCCAGCATCAGTGCACAGTTCCAGA CATCTCTTAACAAGCTGTTGGAGGCGCTGGGCAAGGCTGAGCCCTTCTTCATCCGCTGCATTCGCTCCAA TGCCGAGAAGAAGGAGCTCTGCTTTGATGATGAGCTGGTGCTTCAGCAACTGCGCTACACAGGCATGCTG GAGACCGTGCGCATCCGGCGCTCTGGCTACAGCGCCAAGTACACCTTCCAGGACTTCACGGAGCAGTTCC AGGTGCTGCTGCCCAAGGATGTCCAGCCCTGTAGGGAGGCCATTGCTGCCCTGTTGGAGAAGCTGCAGGT GGACAGGCAGAACTACCAGATCGGGAAGACGAAGGTCTTCCTGAAGGAGACAGAGCGGCAGGCCCTGCAG GAGAGGCTGCATGGTGAGGTCTTACGCAGGATCCTGCTGCTGCAGAGTTGGTTCCGGATGGTGCTGGAAC GCAGGCACTTTGTGCAGATGAAGCATGCTGCCCTGACCATCCAGGCCTGCTGGCGGTCTTATCGTGTGCG CCGTACACTGGAGAGGACGCGGGCAGCTGTGTATCTTCAGGCTGCCTGGAGAGGCTACCTGCAGAGACAG GCCTACCACCACCAGAGGCATAGCATCATCCGCCTGCAGAGCCTCTGCCGTGGCCACCTACAGCGCAGGA GCTTCAGCCAGATGATGTTAGAGAAGCAGAAGGCAGAGCAAGCCAGGGAGACTGCAGGAGCAGAGATGTC AGAGGGAGAGCCCAGCCCTGTGGCCGCTGGGGAGCAGCCGTCTGAGCACCCTGTGGAGGACCCTGAGAGC CTGGGTGTGGAGACTGAGACCTGGATGAACAGCAAGTCCCCAAATGGCTTGTCACCTAAGAAGGAGATCC CCAGCCCGGAGATGGAGACCCCAGCCCAAAAAACAGTGCCAGCTGAAAGTCATGAGAAAGTCCCCAGTAG CCGGGAGAAGCGAGAGTCACGGCGGCAGCGAGGGTTGGAGCATGTTGAACGACAGAACAAACACATCCAA TCCTGCAGGGAGGAGAATAGCACCCTCCGAGAACCTTCCAGAAAGGCAAGCCTGGAAACAGGGGAGAGCT TCCCTGAGGACACAAAGGAGCCCAGAGAAGATGGACTTGAGACATGGACTGAGACCGCAGCCCCCTCTTG TCCAAAGCAGGTGCCGATTGTGGGAGATCCGCCTAGGAGTCCCAGTCCCTTGCAGAGGCCCGCCAGCCTG GACCTAGACAGCAGGGTTAGCCCAGTGCTCCCCAGCAGCTCCCTGGAATCCCCCCAGGATGAGGACAAGG GTGAGAACAGCACCAAGGTTCAGGACAAGCCCGAGAGTCCCAGTGGCTCCACCCAGATCCAACGGTACCA ACACCCGGACACAGAGCGGCTAGCCACTGCTGTGGAGATATGGCGAGGCAAGAAGCTCGCCAGTGCCATG CTGAGCCAATCCCTGGACCTGAGTGAGAAGCCCCGGACTGCAGGGGCAGCCCTGACTCCCACAGAGGAGA GGCGCATCTCCTTCTCCACCAGTGATGTCTCCAAGCTGTCCCCGGTCAAGACTTCAACTGAAGTCGATGG GGATCTGAGCGCCAAGAAGCCAGCCGGCCATAAGAAGAAGTCAGAAGACCCATCTGCTGGTCCCGATGCA GGCCTGCCCACAGGCTCCCAGGGTGACTCTAAATCTGCCTTTAAGCGACTCTTCCTGCACAAAGCCAAGG ATAAGAAGCCCAGCCTGGAGGGTGTGGAGGAGACAGAGGGCAGTGGAGGGCAGGCTGCACAGGAGGCCCC GGCCAGGAAGACTCTAGATGTACCTTCTAGCCAGCAGCACCGCCACACCACAGGCGAGAAGCCTCTAAAA GGGAAGAAGAACCGAAATCGTAAGGTCGGACAGATCACAGTGTCCGAGAAGTGGCGAGAGTCGGTGTTCC GTAAGATCACTAATGCCAACGAGCTCAAGTTTCTGGATGAGTTCCTGCTCAACAAGGTGAATGACCTTCG CTCACAGAAGACACCCATCGAGAGCTTGTTCATTGAGGCCACTGAGCGTTTCAGGAGCAATATCAAGACC ATGTATTCTGTGCCTAACGGGAAGATCCATGTAGGCTACAAGGACTTGATGGAGAACTACCAGATCGTTG TCAGTAACCTGGCTGCCGAGCGTGGGGAGAAGGACACCAACCTGGTCCTCAATGTCTTCCAGTCACTGCT GGATGAATTCACTCGCAGCTACAACAAGACTGACTTTGAGCCGGTCAAGGGCAAAGCCCAGAAGAAGAAG AGGAAGCAGGAGCGTGCTGTCCAGGAACACAATGGACATGTGTTTGCCAGCTACCAAGTGAACATCCCGC AGTCGTGTGAGCAGTGTCTGTCCTACATCTGGCTCATGGACAAGGCCCTGCTATGCAGTGTGTGCAAGAT GACCTGCCACAAGAAATGTGTGCACAAGATTCAGAGCTATTGCTCCTACACTGGACGGAGGAAGAGTGAG CTGGGTGCCGAACCAGGCCACTTCGGCGTGTGTGTAGACAGCCTGACCAGTGACAAGGCCTCCGTGCCCA TTGTGCTGGAGAAGCTTCTGGAACATGTAGAGATGCATGGCCTGTATACCGAGGGTCTTTACCGCAAGTC AGGCGCTGCCAACCGGACACGGGAATTACGCCAGGCTCTGCAGACAGACCCTGCTACAGTTAAGCTGGAG GACTTCCCCATCCACGCCATCACCGGGGTCCTGAAGCAATGGCTGCGGGAGCTGCCTGAGCCACTCATGA CTTTTGCCCAGTATGGAGACTTCCTCAGGGCTGTTGAGCTTCCAGAGAAGCAGGAGCAGCTGGCTGCCAT CTATGCAGTCCTGGACCACCTGCCTGAAGCCAACCACACCTCCCTGGAGAGGCTCATCTTCCACCTTGTC AAAGTGGCCCTGCTTGAAGATGTGAACCGCATGTCTCCGGGAGCTCTAGCTATCATCTTCGCGCCCTGCC TGCTTCGCTGCCCTGACAACTCCGACCCCCTGACCAGCATGAAGGACGTACTAAAGATTACCACGTGTGT GGAGATGCTCATCAAGGAACAGATGAGGAAATACAAGGTGAAGATGGAGGAGATCAACCACCTGGAGGCA GCTGAGAGCATTGCGTTCCGCAGGCTCTCCCTGCTGAGGCAGAATGCCCCGTGGCCTCTCAAACTGGGGT TTTCGTCTCCCTATGAGGGGGTCCGGACCAAAAGCCCCAGGACCCCAGTGGTCCAAGACTTGGAGGAGCT GGGGGCTCTCCCCGAGGAGGCGGCAGGTGGTGACGAGGACCGGGAAAAGGAGATTCTCATGGAGAGGATT CAGTCCATCAAAGAAGAGAAGGAGGACATCACATATCGACTGCCGGAGCTGGACCCACGGGGTTCTGACG AGGAGAACCTTGACTCAGAGACATCAGCCAGCACTGAGAGCCTGCTGGAGGAGAGGGCCGTGCGGGGGGC CGCAGAAGGTCACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271067
Insert Size	6036 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271067.1, NP_001257996.1
RefSeq Size	7077 bp
RefSeq ORF	6036 bp
Locus ID	25486
Gene Summary	displays ATP-dependent binding to actin: binds members of the rho family of GTPases; may play a role in formation of the actin cytoskeleton [RGD, Feb 2006] Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but lacks an alternate internal segment compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Note that the sequence AJ888905.1 is chimeric. Only the Myo9b sequence region was propagated into this RefSeq record.

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