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Cdc14a (NM_001134856) Rat Tagged ORF Clone
CAT＃: RR200086
- TrueORF®
Cdc14a (Myc-DDK-tagged ORF) - Rat CDC14 cell division cycle 14 homolog A (S. cerevisiae) (Cdc14a), transcript variant 1, (10 ug)
"NM_001134856" in other vectors (3)
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CNY 7,700.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR200086 representing NM_001134856
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCGGAGTCAGGGGAACTAATCGGGGCTTGCGAGTTCATGAAAGATCGATTATATTTTGCTACTT TAAGGAATAGACCAAAAAGCACAATAAATATCCACTATTTCTCCATCGACGAGGAGCTGGTCTATGAAAA TTTCTATGCAGATTTTGGACCTCTGAACTTGGCAATGGTGTACAGATACTGCTGTAAGCTAAACAAGAAA CTAAAATCATACAGTTTATCAAGAAAGAAAATAGTGCACTACACCTCTTTCGACCAGCGGAAAAGAGCAA ACGCAGCATTCTTGATAGGTGCTTATGCCGTCATCTACTTAAAGAAGACACCAGAAGAAGCGTACAGAGC TCTCCTGTCTGGCTCAAACCCTCCTTATCTTCCATTCAGGGATGCGTCCTTTGGAAATTGCACTTACAAC CTCACCGTCCTTGACTGTTTACAAGGAATCAGAAAGGGATTACAGCATGGATTTTTTGACTTTGAGACAT TTGATGCGGATGAATATGAACACTATGAGCGAGTTGAAAATGGGGACTTCAACTGGATCGTTCCAGGAAA ATTTTTAGCATTTAGTGGACCACATCCTAAAAGCAAAATTGAAAATGGTTACCCTCTCCACGCTCCTGAA GCCTACTTTCCGTATTTCAAAAAGAACAACGTGACAACAATCGTGAGATTGAATAAAAAGATTTATGAGG CGAAGCGCTTCACAGACGCTGGCTTCGAGCACTACGACCTGTTCTTCATAGATGGCAGCACCCCCAGCGA CAACATCGTGCGAAGATTCCTGAACATCTGTGAGAACACGGAGGGGGCCATTGCGGTCCACTGCAAAGCT GGTCTGGGGAGAACTGGGACTTTGATAGCCTGTTACGTCATGAAACACTACAGGTTCACACACGCTGAAA TCATCGCTTGGATCAGAATTTGTCGACCAGGCTCCATCATTGGACCCCAGCAGCATTTCCTGGAAGAGAA ACAAGCATCATTGTGGGTCCAAGGAGACATTTTTCGATCCAAACTGAAAAATAGACCGTCCAGTGAAGGA AGTATTACTAAAATTATTTCTAGCTTGGATGATATGTCTATTGCTGGGAACTTATCTAAATTACAAAGCA CAGAAAGGATTGGAGAGAATAATTTTGAAGACGAAGATATGGAAATTAAAAACAACGTAACGCAGGGAGA CAAACTACGTGCCTTAAAAAGCCAGAGGCAGCCCCGCTCCTCGCCATCCTGTGCATTTAGGTCAGATGAT ATGAAAGGACACCAAAGGGCAGTGGCCCAGACTTTCAGATTAAGTTCTTCACCACAACCAACAGTGCCGA CTGTGAAGACCCCCAAAGTGTGTTTGTCCCCTTCAGTGACAGCCAAAAGGATAAGCAGAGGTTCTCTGTC TTCAGGAGCAAACATAAGAAGCTTCTCCATAAATTCCCGGCTAGCCAGTTCCCTGGGGAACTTGAATTCT GGGACAGAAGAAACTGAGAACAAAAAGACCACATCACCCATCAAGGCTGCTTTCATAGCCAGCCCGTTCA CCAGCTTCTTGAATGGCAGCACCCAGACACCTGGCAGAAACTACCCTGAGCTCAACAACAATCAGTACAC CAGAAGCAGCAACAGCAGCAGCGGCGGCAGTCTTGGGGGCAACCTGAACAGCTCTCCAAGCGCCAAGCCC GAGGAGCATAACACCATCCTTAGACCTTCCTTCTCGGGGGCGCTCTCTTCCTCCTCAGTGAGATTCCTGA GCCGTTCTATACCTTCCCTTCAGTCTGAATATGTTCATTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200086 representing NM_001134856
Red=Cloning site Green=Tags(s) MAAESGELIGACEFMKDRLYFATLRNRPKSTINIHYFSIDEELVYENFYADFGPLNLAMVYRYCCKLNKK LKSYSLSRKKIVHYTSFDQRKRANAAFLIGAYAVIYLKKTPEEAYRALLSGSNPPYLPFRDASFGNCTYN LTVLDCLQGIRKGLQHGFFDFETFDADEYEHYERVENGDFNWIVPGKFLAFSGPHPKSKIENGYPLHAPE AYFPYFKKNNVTTIVRLNKKIYEAKRFTDAGFEHYDLFFIDGSTPSDNIVRRFLNICENTEGAIAVHCKA GLGRTGTLIACYVMKHYRFTHAEIIAWIRICRPGSIIGPQQHFLEEKQASLWVQGDIFRSKLKNRPSSEG SITKIISSLDDMSIAGNLSKLQSTERIGENNFEDEDMEIKNNVTQGDKLRALKSQRQPRSSPSCAFRSDD MKGHQRAVAQTFRLSSSPQPTVPTVKTPKVCLSPSVTAKRISRGSLSSGANIRSFSINSRLASSLGNLNS GTEETENKKTTSPIKAAFIASPFTSFLNGSTQTPGRNYPELNNNQYTRSSNSSSGGSLGGNLNSSPSAKP EEHNTILRPSFSGALSSSSVRFLSRSIPSLQSEYVHY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001134856 |
| ORF Size | 1791 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001134856.1, NP_001128328.1 |
| RefSeq Size | 4476 bp |
| RefSeq ORF | 1794 bp |
| Locus ID | 310806 |
| MW | 66.9 kDa |
| Gene Summary | The protein encoded by this gene is a dual-specificity phosphatase that preferentially dephosphorylates cyclin dependent kinase substrates to regulate the cell cycle. In human cell lines, this protein localizes to interphase chromosomes, and depletion of the transcript results in centrosome separation and cytokinesis defects. In mouse, the protein localizes to the nucleus of prophase I arrested oocytes and then becomes dispersed in meiotically competent oocytes. Knockdown of the protein delays exit from metaphase I and results in eggs with chromosomal abnormalities and elevated aneuploidy, demonstrating a function in regulation of meiosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN200086 | Cdc14a (untagged ORF) - Rat CDC14 cell division cycle 14 homolog A (S. cerevisiae) (Cdc14a), transcript variant 1, (10 ug) |
CNY 7,700.00 |
|
| RR200086L3 | Lenti ORF clone of Cdc14a (Myc-DDK-tagged ORF) - Rat CDC14 cell division cycle 14 homolog A (S. cerevisiae) (Cdc14a), transcript variant 1, (10 ug) |
CNY 9,500.00 |
|
| RR200086L4 | Lenti ORF clone of Cdc14a (mGFP-tagged ORF) - Rat CDC14 cell division cycle 14 homolog A (S. cerevisiae) (Cdc14a), transcript variant 1, (10 ug) |
CNY 10,450.00 |
