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Gria4 (NM_017263) Rat Tagged ORF Clone
CAT＃: RR200371
- TrueORF®
Gria4 (Myc-DDK-tagged ORF) - Rat glutamate receptor, ionotropic, AMPA4 (Gria4), transcript variant 1, (10 ug)
"NM_017263" in other vectors (3)
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CNY 11,690.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GluA4; GluR-D; GluR4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR200371 representing NM_017263
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGATTATTTGCAGGCAGATTGTCTTGTTGTTTTCTGGATTTTGGGGACTCGCCATGGGAGCCTTTC CAAGCAGCGTTCAAATAGGTGGTCTCTTCATCCGAAACACAGACCAGGAATACACTGCTTTTAGACTGGC AATCTTTCTTCATAACACCAGCCCCAATGCATCGGAAGCTCCTTTCAATTTGGTACCTCATGTGGACAAC ATTGAGACTGCCAACAGTTTTGCTGTGACAAACGCCTTCTGTTCCCAGTATTCTAGAGGGGTGTTTGCCA TTTTTGGACTCTATGACAAGAGATCCGTGCATACCTTGACCTCGTTCTGCAGTGCTCTGCACATCTCTCT CATCACACCAAGCTTTCCCACTGAAGGGGAGAGCCAGTTTGTGCTGCAGCTAAGACCTTCACTGAGAGGT GCACTCCTGAGCCTCCTGGATCACTATGAGTGGAACTGTTTCGTCTTCCTGTATGATACAGACAGGGGGT ATTCAATACTTCAAGCTATAATGGAAAAAGCAGGACAAAATGGATGGCATGTCAGTGCAATATGTGTGGA AAATTTTAATGATGTCAGCTACAGGCAACTGCTAGAAGAGCTTGACAGAAGACAAGAGAAGAAATTTGTG ATAGATTGTGAGATAGAGAGGCTTCAAAACATTTTAGAACAAATTGTGAGTGTTGGGAAGCATGTCAAAG GCTACCATTATATCATCGCAAATTTGGGTTTCAAGGATATTTCTCTTGAGAGATTTATACATGGAGGAGC AAATGTAACAGGATTCCAGTTGGTAGATTTTAATACACCCATGGTAACCAAACTAATGGATCGGTGGAAG AAACTAGATCAGAGAGAATATCCAGGTTCTGAAACACCTCCAAAGTACACCTCTGCTCTCACTTATGATG GAGTCCTGGTGATGGCTGAAACTTTCCGAAGTCTCAGAAGACAGAAAATTGATATTTCAAGGAGAGGAAA TGCTGGGGACTGTCTGGCAAACCCTGCTGCTCCCTGGGGCCAGGGAATTGACATGGAGAGGACACTGAAG CAGGTTCGAATTCAAGGGCTGACTGGGAATGTTCAATTTGACCATTATGGACGTAGAGTTAATTACACAA TGGATGTGTTTGAACTGAAAAGCACAGGACCTCGAAAGGTTGGCTACTGGAATGATATGGATAAATTAGT CTTGATTCAAGATATGCCTACTCTTGGCAATGACACAGCAGCTATTGAGAACAGAACAGTGGTTGTAACC ACAATTATGGAATCTCCCTATGTTATGTACAAGAAAAATCATGAAATGTTTGAAGGAAATGACAAGTACG AAGGCTACTGTGTAGATCTGGCATCGGAAATTGCAAAACATATTGGTATCAAATATAAAATTGCCATTGT TCCTGATGGAAAATATGGAGCAAGGGACGCAGACACTAAGATCTGGAATGGGATGGTAGGAGAGCTTGTG TATGGGAAAGCAGAGATTGCTATTGCCCCTCTGACAATCACATTGGTTCGAGAGGAAGTCATCGATTTTT CTAAGCCTTTTATGAGTTTAGGCATCTCTATCATGATCAAAAAACCTCAGAAATCTAAACCAGGAGTCTT TTCCTTCTTGGACCCTCTGGCCTATGAGATCTGGATGTGCATAGTGTTTGCATACATTGGTGTCAGTGTG GTCTTGTTCCTAGTCAGTAGGTTTAGCCCATATGAGTGGCACACAGAAGAACCTGAGGATGGGAAGGAAG GACCCAGTGACCAGCCTCCCAATGAATTTGGCATCTTTAACAGCCTTTGGTTTTCCCTGGGTGCCTTTAT GCAACAAGGATGTGACATTTCACCCAGATCCCTGTCAGGTCGGATTGTTGGAGGCGTGTGGTGGTTCTTC ACACTCATCATTATATCGTCCTACACTGCTAATCTGGCTGCATTCCTTACTGTGGAGAGAATGGTCTCCC CCATAGAAAGTGCAGAAGACCTGGCCAAACAAACAGAAATTGCCTATGGAACACTTGATTCTGGGTCAAC AAAAGAATTCTTCAGAAGATCAAAAATAGCAGTGTATGAAAAGATGTGGACCTACATGCGATCGGCAGAG CCGTCTGTGTTCACTAGAACTACAGCTGAGGGCGTGGCTCGTGTCCGCAAGTCCAAGGGCAAATTTGCCT TTCTCCTGGAGTCCACGATGAATGAATACATTGAGCAGCGAAAGCCCTGTGACACGATGAAAGTGGGAGG AAACCTGGATTCCAAAGGCTATGGTGTAGCAACGCCCAAGGGTTCCTCATTAAGAACTCCTGTAAACCTT GCCGTTTTGAAACTCAGTGAGGCAGGCGTCTTAGACAAGCTGAAAAACAAATGGTGGTACGATAAAGGTG AATGTGGACCCAAGGACTCGGGAAGCAAGGACAAGACGAGTGCCTTGAGCCTGAGCAACGTAGCAGGCGT CTTCTACATTCTGGTTGGCGGCCTGGGCTTGGCAATGCTGGTGGCTTTGATAGAGTTCTGTTACAAGTCC AGGGCAGAGGCGAAGAGAATGAAGCTGACTTTTTCCGAAGCCATAAGAAACAAAGCCAGGTTATCCATCA CTGGGAGTGTGGGAGAAAACGGCCGTGTGCTGACCCCTGACTGCCCCAAGGCCGTACACACAGGAACTGC AATTAGACAAAGTTCGGGATTGGCTGTCATTGCATCGGACCTACCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200371 representing NM_017263
Red=Cloning site Green=Tags(s) MRIICRQIVLLFSGFWGLAMGAFPSSVQIGGLFIRNTDQEYTAFRLAIFLHNTSPNASEAPFNLVPHVDN IETANSFAVTNAFCSQYSRGVFAIFGLYDKRSVHTLTSFCSALHISLITPSFPTEGESQFVLQLRPSLRG ALLSLLDHYEWNCFVFLYDTDRGYSILQAIMEKAGQNGWHVSAICVENFNDVSYRQLLEELDRRQEKKFV IDCEIERLQNILEQIVSVGKHVKGYHYIIANLGFKDISLERFIHGGANVTGFQLVDFNTPMVTKLMDRWK KLDQREYPGSETPPKYTSALTYDGVLVMAETFRSLRRQKIDISRRGNAGDCLANPAAPWGQGIDMERTLK QVRIQGLTGNVQFDHYGRRVNYTMDVFELKSTGPRKVGYWNDMDKLVLIQDMPTLGNDTAAIENRTVVVT TIMESPYVMYKKNHEMFEGNDKYEGYCVDLASEIAKHIGIKYKIAIVPDGKYGARDADTKIWNGMVGELV YGKAEIAIAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSV VLFLVSRFSPYEWHTEEPEDGKEGPSDQPPNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFF TLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLDSGSTKEFFRRSKIAVYEKMWTYMRSAE PSVFTRTTAEGVARVRKSKGKFAFLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGVATPKGSSLRTPVNL AVLKLSEAGVLDKLKNKWWYDKGECGPKDSGSKDKTSALSLSNVAGVFYILVGGLGLAMLVALIEFCYKS RAEAKRMKLTFSEAIRNKARLSITGSVGENGRVLTPDCPKAVHTGTAIRQSSGLAVIASDLP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017263 |
| ORF Size | 2706 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017263.2, NP_058959.2 |
| RefSeq Size | 4141 bp |
| RefSeq ORF | 2709 bp |
| Locus ID | 29629 |
| UniProt ID | P19493 |
| MW | 100.9 kDa |
| Gene Summary | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing of this gene results in transcript variants encoding different isoforms, which may vary in their signal transduction properties. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN200371 | Gria4 (untagged ORF) - Rat glutamate receptor, ionotropic, AMPA4 (Gria4), transcript variant 1, (10 ug) |
CNY 11,690.00 |
|
| RR200371L3 | Lenti ORF clone of Gria4 (Myc-DDK-tagged ORF) - Rat glutamate receptor, ionotropic, AMPA4 (Gria4), transcript variant 1, (10 ug) |
CNY 13,300.00 |
|
| RR200371L4 | Lenti ORF clone of Gria4 (mGFP-tagged ORF) - Rat glutamate receptor, ionotropic, AMPA4 (Gria4), transcript variant 1, (10 ug) |
CNY 14,730.00 |
