Ptprc (NM_001109888) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CD45; L-CA; Lca; RT7; T200
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR200485 representing NM_001109888 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATTTGTGGCTCAAACTTCTGGCCTTCAGCTTGGCCCTTCTCGGCCCAGAAGTCTTTGTCACAGGGC AAGGAACAACCGACGATGGACTGGACACAACAGAGATTGTTCTTCTGCCTCAAACTGACCCTTTACCTGC TCGCACCACTGAATTCACACCCCCAAGCATCTCTGAAAGAGGAAATGGCTCCTCAGAGACCACATATCTT CCAGGTGCCAACCTCACAACCCCTGCACCATCCACTCTGGGCTTTGCAAGCAATACCACCACAAGCACAG AAATAGCTACCCCTCAAACGAAGCCATCATGTGATGAAAAATTTGGGAACGTTACTGTGCGTTACATCTA TGATGACAGTAGTAAGAATTTTAACGCAAACCTAGAAGGTGACAAAAAACCTAAGTGTGAATATACGGAT TGTGAAAAAGAGTTAAAAAATCTACCAGAATGCTCACAGAAAAACGTCACTCTCTCCAATGGCTCATGTA CTCCAGATAAAATTATAAATTTAGATGTACCACCAGGGACTCACAACTTTAACTTAACAAACTGCACACC AGACATAGAAGCTAATACCTCAATTTGTTTGGAGTGGAAAATAAAAAACAAATTTACCTGCGACATTCAA AAGATTTCATACAATTTCCGTTGTACACCAGAGATGAAAACATTTGCTTTGGACAAACACGGAACACTTT GGTTACACAACCTTACAGTCCGAACAAATTACACATGTGCTGCGGAAGTCCTCTACAATAACGTAATACT TTTAAAACAAGACAGAAGGGTGCAGACTGATTTTGGGACTCCAGAAATGCTTCCCCATGTTCAATGTAAG AATTCAACTAACAGCACAACATTAGTCTCCTGGGCTGAGCCAGCATCTAAACACCATGGATACATTTTAT GCTATAAAAAGACCCCTTCAGAAAAATGTGAAAATTTGGCTAATGATGTGAACAGTTTTGAGGTGAAAAA CCTGAGGCCTTATACAGAGTACACAGTGTCTCTATTTGCCTATGTTATTGGGAGGGTGCAACGAAATGGC CCTGCTAAGGATTGCAACTTTCGGACAAAAGCAGCTCGTCCAGGCAAAGTCAATGGTATGAAAACCTCCC GGGCGTCAGACAATAGTATAAATGTCACGTGTAATTCTCCTTATGAAATTAATGGCCCTGAAGCGCGTTA CATTTTGGAAGTCAAAAGTGGAGGTTCTTTAGTTAAAACTTTCAACCAATCCACATGTAAATTTGTTGTA GACAATCTCTATTATTCAACTGACTATGAGTTTCTGGTCTATTTTTACAATGGAGAGTACCTGGGAGACC CAGAAATAAAACCTCAATCAACATCTTATAATTCTAAAGCACTGATTATATTCCTGGTGTTTCTGATTAT TGTGACATCAATAGCCCTGCTTGTTGTTTTGTATAAAATCTATGATCTGCGTAAGAAAAGATCCAGCAAT TTAGATGAACAGCAGGAACTCGTTGAAAGGGATGAGGAGAAGCAGCTGATAAATGTGGACCCAATTCATT CTGACCTTTTGTTGGAAACATACAAAAGAAAGATTGCCGATGAGGGTAGACTGTTCCTGGCTGAATTTCA GAGCATTCCACGGGTATTCAGCAAGTTTCCCATCAAAGATGCCCGAAAGTCCCAAAACCAGAACAAAAAC CGTTATGTGGACATTCTCCCCTATGATTACAACCGTGTGGAGCTCTCTGAAATAAATGGAGACGCAGGGT CCACCTACATAAATGCCAGCTACATTGATGGCTTCAAGGAACCCAGGAAATACATTGCTGCACAAGGGCC CCGGGATGAGACAGTTGATGACTTCTGGAAGATGATCTGGGAGCAGAAGGCCACAGTTATTGTCATGGTC ACACGATGTGAAGAAGGAAACAGGAACAAGTGTGCAGAATATTGGCCATGCATGGAGGAAGGCACTCGGA CTTTCAGAGATGTTGTCGTGACGATCAATGACCACAAACGATGTCCTGATTACATTATCCAGAAGCTGAG CATTGCCCACAAAAAAGAAAAAGCAACTGGAAGAGAAGTGACTCATATTCAATTCACCAGTTGGCCAGAC CATGGGGTTCCTGAAGACCCTCACCTGCTCCTGAAACTTCGACGGAGAGTTAATGCTTTTAGCAACTTCT TCAGTGGACCCATTGTGGTGCACTGCAGTGCTGGCGTTGGGCGTACAGGCACCTACATTGGAATTGATGC CATGCTCGAAAGCCTAGAAGCAGAGGGCAAAGTGGATGTCTATGGCTATGTTGTCAACCTAAGGCGACAG AGATGTCTGATGGTGCAAGTGGAGGCCCAGTACATCCTGATCCATCAGGCCTTAGTGGAGTACAATCAAT TTGGGGAAACAGAAGTGAACTTGTCTGAGTTACATTCATGTCTACAGAATCTGAAGAAGAGAGATCCACC CAGTGACCCGTCTCCTTTGGAGGCTGAGTACCAGAGACTTCCTTCATACAGGAGCTGGAGGACACAGCAC ATTGGAAATCAAGAAGAAAATAAAAAGAAAAACAGGAGTTCTAACGTTGTTCCATATGACTTTAACAGAG TGCCACTTAAGCATGAACTAGAGATGAGCAAAGAGAGCGAGGCTGAATCCGACGAATCTTCAGATGAGGA CAGTGACTCGGAAGAAACCAGCAAATACATTAATGCGTCATTTGTGATGAGTTACTGGAAACCAGAAATG ATGATTGCTGCCCAGGGTCCACTAAAAGAGACTATTGGTGACTTTTGGCAGATGATATTCCAAAGAAAAG TCAAGGTTATTGTGATGTTGACAGAGTTAATGAGTGGAGACCAGGAAGTCTGTGCTCAATACTGGGGAGA AGGAAAGCAGACTTATGGAGACATGGAAGTAATGTTGAAAGACACGAACAAATCCTCAGCCTATATTCTG CGAGCATTTGAACTGAGACATTCCAAGAGAAAGGAGCCTAGAACTGTGTACCAGTACCAGTGTACCACAT GGAAAGGGGAGGAGCTCCCTGCAGAACCCAAAGATCTAGTGACTCTGATTCAGAACATCAAACAGAAGCT TCCCAAGAGTGGCTCAGAAGGGATGAAGTACCACAAGCATGCATCAATCCTAGTCCACTGCAGGGATGGA TCCCAGCAGACGGGGTTGTTCTGTGCTCTGTTCAATCTCCTGGAAAGTGCAGAAACAGAAGATGTGGTTG ATGTTTTTCAAGTGGTAAAGTCTCTACGCAAAGCACGGCCGGGGATGGTGGGCAGCTTTGAGCAATACCA GTTCCTCTATGACATCATGGCCAGCATCTATCCCACCCAGAATGGACAAGTGAAGAAAGCAAACAGCCAA GACAAAATTGAATTTCATAACGAAGTGGACGGAGCCAAGCAGGACGCAAACTGTGTTCAGCCAGCTGATC CTCTGAACAAAGCCCAGGAAGACAGCAAAGAAGTTGGAGCTTCAGAGCCTGCAAGTGGTTCTGAGGAGCC AGAACATTCTGCAAATGGTCCCATGAGCCCAGCTCTAACCCCGAGCTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR200485 representing NM_001109888 Red=Cloning site Green=Tags(s) MYLWLKLLAFSLALLGPEVFVTGQGTTDDGLDTTEIVLLPQTDPLPARTTEFTPPSISERGNGSSETTYL PGANLTTPAPSTLGFASNTTTSTEIATPQTKPSCDEKFGNVTVRYIYDDSSKNFNANLEGDKKPKCEYTD CEKELKNLPECSQKNVTLSNGSCTPDKIINLDVPPGTHNFNLTNCTPDIEANTSICLEWKIKNKFTCDIQ KISYNFRCTPEMKTFALDKHGTLWLHNLTVRTNYTCAAEVLYNNVILLKQDRRVQTDFGTPEMLPHVQCK NSTNSTTLVSWAEPASKHHGYILCYKKTPSEKCENLANDVNSFEVKNLRPYTEYTVSLFAYVIGRVQRNG PAKDCNFRTKAARPGKVNGMKTSRASDNSINVTCNSPYEINGPEARYILEVKSGGSLVKTFNQSTCKFVV DNLYYSTDYEFLVYFYNGEYLGDPEIKPQSTSYNSKALIIFLVFLIIVTSIALLVVLYKIYDLRKKRSSN LDEQQELVERDEEKQLINVDPIHSDLLLETYKRKIADEGRLFLAEFQSIPRVFSKFPIKDARKSQNQNKN RYVDILPYDYNRVELSEINGDAGSTYINASYIDGFKEPRKYIAAQGPRDETVDDFWKMIWEQKATVIVMV TRCEEGNRNKCAEYWPCMEEGTRTFRDVVVTINDHKRCPDYIIQKLSIAHKKEKATGREVTHIQFTSWPD HGVPEDPHLLLKLRRRVNAFSNFFSGPIVVHCSAGVGRTGTYIGIDAMLESLEAEGKVDVYGYVVNLRRQ RCLMVQVEAQYILIHQALVEYNQFGETEVNLSELHSCLQNLKKRDPPSDPSPLEAEYQRLPSYRSWRTQH IGNQEENKKKNRSSNVVPYDFNRVPLKHELEMSKESEAESDESSDEDSDSEETSKYINASFVMSYWKPEM MIAAQGPLKETIGDFWQMIFQRKVKVIVMLTELMSGDQEVCAQYWGEGKQTYGDMEVMLKDTNKSSAYIL RAFELRHSKRKEPRTVYQYQCTTWKGEELPAEPKDLVTLIQNIKQKLPKSGSEGMKYHKHASILVHCRDG SQQTGLFCALFNLLESAETEDVVDVFQVVKSLRKARPGMVGSFEQYQFLYDIMASIYPTQNGQVKKANSQ DKIEFHNEVDGAKQDANCVQPADPLNKAQEDSKEVGASEPASGSEEPEHSANGPMSPALTPSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001109888
ORF Size	3549 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001109888.2
RefSeq Size	4797 bp
RefSeq ORF	3552 bp
Locus ID	24699
UniProt ID	P04157
MW	134.2 kDa
Gene Summary	member of a family of heavily glycosylated leukocyte cell surface glycoproteins; displays extensive O-glycosylation [RGD, Feb 2006]

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