Plcb2 (NM_053478) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR202153 representing NM_053478 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTTGCTCAACCCTGTCCTGTTGCCCCCTAAGGTGAAGGCATATCTGAGCCAAGGGGAGCGCTTCA TCAAGTGGGACGATGAAACCTCAATAGCCTCCCCTGTTATCCTTCGGGTGGATCCCAAGGGCTATTACTT ATACTGGACACATCAAAGTAAGGAGATGGAATTTCTGGATGTCACGAGCATCCGAGACACTCGCTTTGGG AAGTTTGCCAAGATACCCAAGAGCCAGAAACTCCGGGAGGTATTCAACATGGACTTTCCAGACAACCACT TCCTGCTGAAAACATTCACAGTGGTATCCGGCCCGGACATGGTGGGCCTCACCTTCCACAATTTTGTCTC TTACAAGGAGAACGTAGGCAAGGATTGGGCTGAGGATGTACTGGCTCTGGCCAAACACCCAATGACAGCC AATGCTTCTCGAAGCACATTCCTAGACAAGATCCTGGTGAAGCTTAAAATGCAGCTGAGCCCTGAAGGGA AGATCCCTGTGAAGAATTTTTTCCAGATGTTTCCTGCCGATCGCAAACGTGTAGAAGCTGCCCTCAGTGC CTGTCACCTTGCAAAAGGCAAGAATGATGCTATCAATCCCGAGGACTTCCCAGAATCTGTGTACAAGAGC TTCCTCATGAGCCTCTGTCCTCGGCCAGAAATTGATGAGATCTTCACTTCTTATCATGCTAAAGCCAAAC CCTACATGACCAAGGAGCACCTGACCAAATTCATCAATCAGAAGCAACGAGACCCTCGACTCAATTCCTT GCTGTTCCCACCAGCCCGGCCTGAGCAGGTGCAGGCGCTCATTGACAAGTACGAGCCCAGCGGCATCAAT GTGCAGAGGGGCCAACTGTCACCAGAGGGCATGGTCTGGTTTCTCTGTGGACCAGAGAACAGTGTCCTGG CCCATGATACTCTGCGGATCCACCAAGACATGACACAGCCTCTGAATCACTACTTCATCAACTCCTCACA CAACACTTACCTGACAGCTGGCCAGTTTTCAGGCCCTTCCTCGGCTGAGATGTACCGCCAGGTGCTGCTA TCTGGCTGCCGTTGTGTAGAATTAGATTGTTGGAAAGGAAAGCCCCCCGACGAAGAACCCATCATCACCC ACGGCTTCACTATGACCACAGATATCTTGTTCAAGGAAGCAGTCGAAGCCATTGCAGAAAGTGCCTTTAA GACCTCCCCATATCCTGTCATCCTGTCATTTGAAAACCACGTGGACTCACCCCGCCAACAGGCTAAGATG GCCGAGTACTGCCGGACCATGTTTGGAGAGACCTTGCTCACAGAGCCCCTGGAAAACTTTCCTCTGAAAC CTGGCATGCCTCTGCCTAGCCCTGAGGACCTCCGGGGCAAGATCCTCATTAAGAATAAGAAGAACCAGTT TTCTGGGCCAGCATCCCCCAACAAGAAGCCTGATGGGGTGTCTGAGGGCGGCTTCCCATCTAGTGTCCCT GTAGAAGAGGACACGGGGTGGACTGCTGAGGACCGGACTGAGGTGGAGGAGGGAGAGGAGGAGGAGGAGG TGGAAGAAGAGGAAGAGGAGGAGTCAGGAAACCTGGATGAAGAAGAAATTAAGAAGATGCAGTCTGATGA GGGCACAGCAGGCTTGGAGGTGACAGCTTATGAGGAAATGTCCAGCCTTGTCAATTACATCCAGCCCACC AAGTTTATCTCCTTCGAGTTCTCTGCACAGAAGAACAGGAGTTACCTTGTCTCTTCCTTCACGGAGCTCA AGGCCTACGAGCTGCTCTCCAAGGCCTCCATGCAGTTTGTGGACTATAATAAACGCCAGATGAGCCGCGT GTACCCCAAGGGCACGCGCATGGACTCTTCCAACTACATGCCCCAGATGTTCTGGAATGCCGGATGTCAG ATGGTTGCCCTCAATTTCCAGACAATGGATCTGCCCATGCAGCAGAACATGGCACTGTTTGAGTTCAACG GGCAGAGTGGCTACCTCCTAAAGCACGAGTTCATGCGCAGGCAGGACAAGCAGTTCAACCCCTTCTCGGT GGACCGCATTGATGTGGTGGTTGCCACTACCCTTTCCATTACGGTCATCTCTGGGCAGTTCCTGTCAGAG CGCAGTGTGCGCACCTATGTGGAAGTAGAACTGTTTGGCCTCCCAGGGGACCCCAAGAGGCGCTATCGTA CCAAGCTGTCACCTACTGCTAACTCCATCAATCCTGTCTGGAAGGAGGAACCCTTCATTTTTGAGAAGAT CTTGGTGCCTGAGCTGGCCTCCCTCAGGATAGCTGTAATGGAAGAAGGCGGCAAATTTATTGGACATCGC ATCATCCCCATCAATGCCTTACATTCCGGATACCACCATCTGTGCCTGCGCAGTGAGAGCAACATGCCTC TCACCATGCCTGCTCTCTTCGTCTTCCTGGAGATGAAGGACTATGTACCTGACACGTGGGCAGATCTTAC TGTGGCTCTTGCCAACCCCATCAAGTACTTCAGTGCCCATGATAAGAAGTCTGTGAAGCTCAAAGAAGTG ACAGGAAGTCTGCCTGAGAAGCTCTTCTCTGGGATTCCTGTTGCCAGCCAGTCCAACGGGGCTCCAGTCT CCGCAGGCAATGGGTCAACAGCGCCTGGGACCAAGGCCAAGGAGGAAGCTACGAAGGAAGTGGCAGAGCC ACAGACCACTAGCTTGGAAGAGCTGCGGGAGCTGAAGGGCGTGGTGAAGCTGCAGCGGAGACATGAGAAG GAGCTTCGAGAGCTGGAGCGGCGTGGAGCCCGGCGCTGGGAGGAGCTGCTGCAGCGTGGCGCTGCACAGC TAGCGGAGCTCCAGGACCCAGCAGCAAGCTGCAAGCTCCGCCCGGGCAAGGGCTCCCGCAAGAAGAGGAT CGTGCCCTGTGAGGAGACCATCGTGGTGCCTAGGGAGGTCCTCGAGGGACCGGACCCCCGCGTGCAGGAT CTGAAGGACAGGCTGGAGCAGGAGCTGCAGCAGCAAGGCGAGGAGCAGTACCGCTCTGTCCTCAAGCGTA AGGAGCAGCACGTGACCGAGCAAATCGCCAAGATGATGGAGCTGGCCAGAGAGAAACAGGCTGCTGAACT CAAGAGCTTCAAGGAGACCTCGGAAACTGACACCAAAGAGATGAAGAAAAAACTGGAGGCCAAAAGGCTG GAGCGGATCCAAGCCATGACCAAAGTCACCACAGACAAGGTGGCCCAGGAGAGGCTCAAGAGAGAAATTA ACAACTCCCACATCCAGGAGGTGGTGCAGGCTGTCAAGCAGATGACAGAGACCCTGGAGCGTCACCAGGA GAAGCTGGAAGAGAAGCAGACAGCCTGCCTGGAGCAGATCCAGGCAATGGAAAAGCAGTTCCAGGAGAAG GCGCTGGCAGAGTATGAGGCCAAGATGAAGGGCCTGGAGGCTGAAGTAAAGGAGTCAATGCGGGCTTGCT TCAAGGCCTGCTTCCCCACGGAAGCAGAAGAGAAGCCTGAGAGGCCCTGTGAGGCCTCTGAGGAGTCGTG TCCACAGGAGCCACTTGTGAACAAGACAGACACTCAGGAGAGCCGCCTCTCCACAGGAGCCACTTGTGAA CAAGACAGACACTCAGGAGAGCCGCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR202153 representing NM_053478 Red=Cloning site Green=Tags(s) MSLLNPVLLPPKVKAYLSQGERFIKWDDETSIASPVILRVDPKGYYLYWTHQSKEMEFLDVTSIRDTRFG KFAKIPKSQKLREVFNMDFPDNHFLLKTFTVVSGPDMVGLTFHNFVSYKENVGKDWAEDVLALAKHPMTA NASRSTFLDKILVKLKMQLSPEGKIPVKNFFQMFPADRKRVEAALSACHLAKGKNDAINPEDFPESVYKS FLMSLCPRPEIDEIFTSYHAKAKPYMTKEHLTKFINQKQRDPRLNSLLFPPARPEQVQALIDKYEPSGIN VQRGQLSPEGMVWFLCGPENSVLAHDTLRIHQDMTQPLNHYFINSSHNTYLTAGQFSGPSSAEMYRQVLL SGCRCVELDCWKGKPPDEEPIITHGFTMTTDILFKEAVEAIAESAFKTSPYPVILSFENHVDSPRQQAKM AEYCRTMFGETLLTEPLENFPLKPGMPLPSPEDLRGKILIKNKKNQFSGPASPNKKPDGVSEGGFPSSVP VEEDTGWTAEDRTEVEEGEEEEEVEEEEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNYIQPT KFISFEFSAQKNRSYLVSSFTELKAYELLSKASMQFVDYNKRQMSRVYPKGTRMDSSNYMPQMFWNAGCQ MVALNFQTMDLPMQQNMALFEFNGQSGYLLKHEFMRRQDKQFNPFSVDRIDVVVATTLSITVISGQFLSE RSVRTYVEVELFGLPGDPKRRYRTKLSPTANSINPVWKEEPFIFEKILVPELASLRIAVMEEGGKFIGHR IIPINALHSGYHHLCLRSESNMPLTMPALFVFLEMKDYVPDTWADLTVALANPIKYFSAHDKKSVKLKEV TGSLPEKLFSGIPVASQSNGAPVSAGNGSTAPGTKAKEEATKEVAEPQTTSLEELRELKGVVKLQRRHEK ELRELERRGARRWEELLQRGAAQLAELQDPAASCKLRPGKGSRKKRIVPCEETIVVPREVLEGPDPRVQD LKDRLEQELQQQGEEQYRSVLKRKEQHVTEQIAKMMELAREKQAAELKSFKETSETDTKEMKKKLEAKRL ERIQAMTKVTTDKVAQERLKREINNSHIQEVVQAVKQMTETLERHQEKLEEKQTACLEQIQAMEKQFQEK ALAEYEAKMKGLEAEVKESMRACFKACFPTEAEEKPERPCEASEESCPQEPLVNKTDTQESRLSTGATCE QDRHSGEPP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053478
ORF Size	3549 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq Size	3999 bp
RefSeq ORF	3552 bp
Locus ID	85240
UniProt ID	O89040
MW	134.9 kDa
Gene Summary	may play a role in inositol mediated signal transduction of bitter taste perception [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.