Cacng8 (NM_080696) Rat Tagged ORF Clone

CAT#: RR202459

  • TrueORF®

Cacng8 (Myc-DDK-tagged ORF) - Rat calcium channel, voltage-dependent, gamma subunit 8 (Cacng8), (10 ug)



  "NM_080696" in other vectors (3)

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CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR202459 representing NM_080696
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

CTGGAATCATTGAAACGCTGGAATGAAGAGAGGGGTTTGTGGTGCGAAAAGGGCGTTCAGGTACTACTGA
CCACCATAGGCGCCTTTGCAGCTTTTGGCCTCATGACCATCGCCATCAGCACTGACTACTGGCTCTACAC
AAGAGCTCTCATCTGCAACACCACCAACCTCACAGCAGGTGATGATGGACCACCCCATCGTGGGGGCAGT
GGCTCCTCAGAGAAGAAGGACCCTGGGGGCCTCACACATTCAGGCCTCTGGCGGATATGCTGCCTGGAAG
GGTTGAAAAGAGGTGTCTGCGTGAAGATCAACCACTTCCCGGAGGACACGGACTACGACCACGACAGCGC
GGAGTACCTGCTCCGAGTAGTCCGGGCCTCCAGCATCTTTCCTATCCTGAGCGCCATCCTGCTGCTGCTC
GGGGGCGTGTGCGTAGCTGCCTCTCGCGTCTACAAATCCAAAAGGAACATCATCCTGGGCGCAGGGATCC
TGTTCGTGGCAGCAGGCCTGAGCAACATCATCGGGGTGATCGTGTACATATCGGCAAACGCGGGCGAGCC
AGGCCCCAAGAGGGACGAGGAGAAGAAAAACCACTATTCGTATGGCTGGTCCTTCTACTTCGGCGGGCTG
TCATTCATCCTGGCCGAGGTGATCGGCGTGCTAGCCGTCAACATCTACATCGAGCGCAGCCGCGAGGCAC
ACTGCCAATCACGCTCGGACCTACTCAAGGCCGGCGGCGGCGCGGGCGGCAGTGGCGGGAGCGGCCCCTC
GGCCATCCTCCGTCTGCCCAGTTACCGCTTCCGCTACCGCCGCCGCTCCCGCTCCAGCTCCCGAGGCTCC
AGCGAGGCCTCGCCATCGCGGGATGCGTCTCCCGGCGGCCCCGGGGGCCCGGGCTTCGCCTCCACGGACA
TCTCCATGTACACGCTCAGTCGCGACCCGTCCAAGGGCAGCGTGGCTGCGGGGCTGGCGAGCGCCGGGGG
TGGAGGCGGCGGTGCCGGCGTGGGTGCCTACGGCGGGGCGGCCGGGGCAGCGGGGGGCGGCGGGACGGGC
TCGGAGCGGGACCGAGGGAGCTCAGCGGGCTTCCTCACGCTGCACAACGCCTTCCCCAAGGAGGCGGCGT
CCGGCGTCACGGTCACGGTCACCGGACCGCCCGCTGCGCCGGCGCCCGCGCCGCCCGCTCCTGCAGCGCC
CGCGCCCGGGACGCTGTCCAAAGAGGCCGCCGCGTCCAACACCAACACGCTCAACAGGAAAACCACGCCC
GTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR202459 representing NM_080696
Red=Cloning site Green=Tags(s)

LESLKRWNEERGLWCEKGVQVLLTTIGAFAAFGLMTIAISTDYWLYTRALICNTTNLTAGDDGPPHRGGS
GSSEKKDPGGLTHSGLWRICCLEGLKRGVCVKINHFPEDTDYDHDSAEYLLRVVRASSIFPILSAILLLL
GGVCVAASRVYKSKRNIILGAGILFVAAGLSNIIGVIVYISANAGEPGPKRDEEKKNHYSYGWSFYFGGL
SFILAEVIGVLAVNIYIERSREAHCQSRSDLLKAGGGAGGSGGSGPSAILRLPSYRFRYRRRSRSSSRGS
SEASPSRDASPGGPGGPGFASTDISMYTLSRDPSKGSVAAGLASAGGGGGGAGVGAYGGAAGAAGGGGTG
SERDRGSSAGFLTLHNAFPKEAASGVTVTVTGPPAAPAPAPPAPAAPAPGTLSKEAAASNTNTLNRKTTP
V

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_080696
ORF Size 1263 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_080696.2, NP_542427.1
RefSeq Size 1266 bp
RefSeq ORF 1266 bp
Locus ID 140729
UniProt ID Q8VHW5
MW 43.3 kDa
Gene Summary Voltage-dependent calcium channels couple membrane depolarization in a number of cellular processes. These activities are regulated by distinct channels composed of the pore-forming alpha-1 subunit and the modulatory beta, alpha-2/delta and gamma subunits. The protein encoded by this gene represents one of the gamma subunits. It is an integral membrane protein that is thought to stabilize the calcium channel in an inactive (closed) state. This gene is located in a cluster with two similar gamma subunit-encoding genes on chromosome 1. The mRNA for this gene is believed to initiate translation from a non-AUG (CUG) start codon. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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