Mtr (NM_030864) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	methioninesynthase
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR203085 representing NM_030864 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAAAACCCTGCAGGATGAGATTGAAGCCATTCTTCGGAAGAGGATTATGGTGCTAGATGGCGGGA TGGGGACCATGATCCAGCGGTACAAACTAAGCGAAGAAAATTTCCAAGGTCAAGAGTTTAAAGACCACAG CAGGCCACTGAAAGGCAACAATGACATCTTGAGTATAACTCAGCCTGATGTTATTTACCAGATTCATAAG GAATACTTGCTGGCTGGAGCAGACATCATTGAAACAAACACTTTCAGCAGCACTAGTATCGCCCAGGCTG ACTATGGCCTCGAACACTTGGCCTACCGGATGAACAAATGCTCCGCAGACGTGGCCAGAAAAGCCGCCGA GGAGATAACTCTGCAGACAGGAGTCAAGAGGTTTGTGGCTGGATCTCTGGGTCCGACTAACAAGACACTT TCAGTTTCCCCATCTGTGGAAAGGCCAGATTACAGGAACATCACATTTGATGAGCTTGTTGAAGCATACC AGGAGCAGGCCAAGGGACTGCTGGATGGTGGGGTTGACATCTTACTCATTGAGACGATTTTTGATACAGC CAATGCCAAAGCAGCCTTGTTTGCGCTCCAGAAACTCTTTGAAGAGAATTATGCTTCTCCCAGGCCTATC TTTATTTCTGGGACCATTGTTGATAAAAGTGGACGGACTCTTTCTGGCCAGACAGGAGAGGCTTTTGTCA CCAGCGTGTCTCATTCAGACCCCCTGTGCATTGGATTAAATTGTGCTCTGGGTGCAGCTGAAATGAGGCC TTTCATTGAAACAATTGGAAAATGTACAACAGCTTATGTCCTCTGTTACCCAAATGCAGGTCTTCCCAAT ACCTTTGGTGATTATGATGAAACACCAGCCATGATGGCCATGCACCTGAAGGACTTTGCCGTGGACGGCT TGGTGAATGTAGTTGGTGGATGCTGTGGTTCAACGCCTGATCACATCAGGGAAATTGCAGAAGCTGTGAA AAATTGTAAGCCAAGAGTTCCCCCTGATAGTGTTTTTGAAGGACATATGTTACTGTCTGGTCTAGAGCCC TTCAGGATTGGACCATACACTAACTTTGTTAACATTGGAGAGCGCTGTAACGTGGCAGGATCCAAGAAAT TTGCTAAGCTCATCATGGCAGGAAACTATGAAGAAGCCCTGAGCGTTGCCAAAGTGCAGGTGGAAATGGG AGCTCAGGTGCTGGATATCAACATGGATGACGGCATGCTGGATGGTCCCAGTGCGATGACCAAATTTTGC AACTTCATTGCTTCGGAGCCTGACATTGCCAAGGTGCCCCTGTGCATTGACTCTTCCAATTTTGCTGTGA TCGAGGCGGGGTTGAAGTGCTGCCAAGGGAAGTGCATAGTCAACAGCATCAGCCTGAAGGAGGGGGAGGA GGACTTCCTGGAGAAGGCCAGGAAGATTAAGAAGTTTGGAGCTGCTGTGGTGGTTATGGCTTTTGATGAG GAAGGACAGGCAACAGAAACAGATGTTAAAGTCAGTGTGTGCACTAGAGCCTACCATCTACTTGTGGAAA AAGTGGGCTTCAATCCAAATGATATCATCTTTGACCCCAACATCCTTACCATTGGAACTGGAATGGAAGA ACATAACTTGTATGCTATCAATTTTATTCATGCAACGAGAGTCATTAAGGAAACATTACCAGGAGTCAGA ATAAGTGGAGGTCTCTCCAACCTATCCTTCGCCTTCCGAGGGATGGACGCCATTCGAGAAGCAATGCACG GTGTTTTCCTTTATCATGCAATCAAGTTCGGTATGGACATGGGGATAGTCAATGCCGGCAGTCTCCCCGT GTACGATGATATCCATAAGGACCTTCTTCAGCTCTGTGAAGACCTCATCTGGAACAGAGACGCTGAGGCT ACTGAGAAACTCTTACGATATGCCCAGACTCATGGCAAAGGAGGGAAGAAAGTCATCCAGACAGATGAGT GGAGGAATGGCTCGATTGAAGAGCGCCTGGAGTATGCTCTTGTGAAGGGCATTGAAAAGCACATTGTTGA AGACACTGAAGAAGCCAGGTTAAACCGGGAAAAATACCCCCGGCCTCTGAATATAATTGAAGGGCCCCTG ATGAATGGCATGAAAGTGGTTGGTGATCTTTTTGGAGCTGGAAAAATGTTTCTACCTCAGGTTATAAAGT CAGCCCGTGTCATGAAGAAGGCTGTTGGCCACCTTATCCCTTTCATGGAAAAAGAAAGAGAAGAAGCCAG GGTGCTTAATGGTTCGGTTGAAGAAGAGGACCCTTACCAAGGCACCATTGTGCTGGCCACTGTTAAAGGT GATGTGCATGACATTGGCAAGAACATTGTTGGTGTGGTGCTGGGCTGTAATAATTTCAGAGTTATTGATT TAGGAGTCATGACTCCCTGTGATAAGATACTTCAAGCTGCTCTGGACCACAAAGCAGATATAATTGGCTT GTCAGGACTTATCACGCCATCCTTGGATGAAATGATTTTTGTTGCCAAGGAAATGGAGAGGTTGGCTATA AAGATTCCATTGTTGATAGGAGGAGCAACCACTTCAAGAACCCACACAGCAGTTAAAATAGCACCACGGT ACAGTGCGCCTGTAATCCATGTTCTAGATGCATCCAAGAGTGTGGTGGTGTGTTCTCAGCTGTTAGATGA AAATCTGAAAGATGACTACTTTGAAGAAATACTGGAAGAGTATGAAGATATTAGACAGGACCACTACGAG TCTCTCAAAGAGAGAAAATACTTACCCCTAAGTCAAGCCAGAAAACACAGTTTCCACATTGATTGGCTGT CTGAACCTCACCCAGTGAAGCCCACGTTTATTGGGACCCAGGTCTTTGAAGACTACAACCTGCAAAAGCT GGTGGACTACATTGACTGGAAGCCTTTCTTTGATGTCTGGCAGCTCCGCGGCAAGTACCCGAACCGAGGA TTTCCCAAGATATTCAATGACAAAGCAGTAGGTGAAGAAGCCAGAAAGGTATATGAGGATGCTCAGAACA TGCTGAGCATACTGATCAGTCGAAAGAAACTCCGGGCCAGGGGTGTGGTTGGATTCTGGCCAGCACAGAG CGTCCAAGATGACATCCACCTGTATGCAGAGGGGGCGGTGCCCCAGGCCGCCGAGCCCATAGCCACCTTC TATGGACTGAGGCAGCAGGCTGAGAAGGACTCCTCCAGTACAGACCCCTACCACTGCCTCTCAGACTTCG TTGCTCCTCTGCATTCTGGTGTCCGTGACTACTTGGGTCTGTTTGCTGTGGCCTGCTTTGGGGTTGAAGA GCTGAGTAAGGCCTATGAGGATGATGGCGATGACTACAGCAGCATCATGGTGAAGGCCCTGGGGGACAGG CTGGCGGAGGCCTTTGCAGAGGAGCTCCACGAGAGAGTGCGCAGAGAACTCTGGGCTTACTGTGGGAGTG AGCAGTTGGGTGTCACAGACTTGCGCAAACTCCGCTATGAGGGCATCCGGCCAGCTCCTGGTTACCCCAG CCAGCCTGACCATACCGAGAAGCTCACCATGTGGAGGCTGGCCAACATCGAGCAGGCCACAGGCATCAGG TTGACAGAATCCTTGGCAATGGCACCTGCTTCAGCAGTGTCGGGTCTCTACTTCTCCAATGTAAAGTCCA AATATTTTGCTGTGGGGAAAATTTCCAAAGATCAGATTGAGGATTATGCCTTGAGGAAGAACATGTCAGT GGCCGAGGTGGAAAAATGGCTTGGCCCCATTCTGGGATATGATACAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR203085 representing NM_030864 Red=Cloning site Green=Tags(s) MKKTLQDEIEAILRKRIMVLDGGMGTMIQRYKLSEENFQGQEFKDHSRPLKGNNDILSITQPDVIYQIHK EYLLAGADIIETNTFSSTSIAQADYGLEHLAYRMNKCSADVARKAAEEITLQTGVKRFVAGSLGPTNKTL SVSPSVERPDYRNITFDELVEAYQEQAKGLLDGGVDILLIETIFDTANAKAALFALQKLFEENYASPRPI FISGTIVDKSGRTLSGQTGEAFVTSVSHSDPLCIGLNCALGAAEMRPFIETIGKCTTAYVLCYPNAGLPN TFGDYDETPAMMAMHLKDFAVDGLVNVVGGCCGSTPDHIREIAEAVKNCKPRVPPDSVFEGHMLLSGLEP FRIGPYTNFVNIGERCNVAGSKKFAKLIMAGNYEEALSVAKVQVEMGAQVLDINMDDGMLDGPSAMTKFC NFIASEPDIAKVPLCIDSSNFAVIEAGLKCCQGKCIVNSISLKEGEEDFLEKARKIKKFGAAVVVMAFDE EGQATETDVKVSVCTRAYHLLVEKVGFNPNDIIFDPNILTIGTGMEEHNLYAINFIHATRVIKETLPGVR ISGGLSNLSFAFRGMDAIREAMHGVFLYHAIKFGMDMGIVNAGSLPVYDDIHKDLLQLCEDLIWNRDAEA TEKLLRYAQTHGKGGKKVIQTDEWRNGSIEERLEYALVKGIEKHIVEDTEEARLNREKYPRPLNIIEGPL MNGMKVVGDLFGAGKMFLPQVIKSARVMKKAVGHLIPFMEKEREEARVLNGSVEEEDPYQGTIVLATVKG DVHDIGKNIVGVVLGCNNFRVIDLGVMTPCDKILQAALDHKADIIGLSGLITPSLDEMIFVAKEMERLAI KIPLLIGGATTSRTHTAVKIAPRYSAPVIHVLDASKSVVVCSQLLDENLKDDYFEEILEEYEDIRQDHYE SLKERKYLPLSQARKHSFHIDWLSEPHPVKPTFIGTQVFEDYNLQKLVDYIDWKPFFDVWQLRGKYPNRG FPKIFNDKAVGEEARKVYEDAQNMLSILISRKKLRARGVVGFWPAQSVQDDIHLYAEGAVPQAAEPIATF YGLRQQAEKDSSSTDPYHCLSDFVAPLHSGVRDYLGLFAVACFGVEELSKAYEDDGDDYSSIMVKALGDR LAEAFAEELHERVRRELWAYCGSEQLGVTDLRKLRYEGIRPAPGYPSQPDHTEKLTMWRLANIEQATGIR LTESLAMAPASAVSGLYFSNVKSKYFAVGKISKDQIEDYALRKNMSVAEVEKWLGPILGYDTD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_030864
ORF Size	3759 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_030864.1, NP_110491.1
RefSeq Size	4498 bp
RefSeq ORF	3762 bp
Locus ID	81522
UniProt ID	Q9Z2Q4
MW	139.2 kDa
Gene Summary	catalyzes the cobalamin-dependent conversion of 5-methyltetrahydrofolate and L-homocysteine to tetrahydrofolate and L-methionine [RGD, Feb 2006]

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