United States
Germany
Japan
United Kingdom
China
Man2b1 (NM_199404) Rat Tagged ORF Clone
CAT＃: RR204232
- TrueORF®
Man2b1 (Myc-DDK-tagged ORF) - Rat mannosidase, alpha, class 2B, member 1 (Man2b1), (10 ug)
"NM_199404" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 16,250.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Manb |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR204232 representing NM_199404
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCACCGGATCGCTTGCCCCTGGTGTCCGTGCTGGTGGCGGGAACACTGGGTGGCTCTGGATGAGCT CCTGCAACTTAGGTTTGCCAGTCCTGTCTATTTCTTTTCTTATTTGGTTGTTACTGGCTGCGCCTGGCGC TCAGGCCGCGGGTTACAAGACATGTCCCACCACGAAGCCAGGCATGCTCAACGTGCACCTGCTGCCTCAC ACACACGATGATGTGGGCTGGCTGAAGACAGTAGACCAATACTACTACGGCATCATGAGTGACGTCCAGC ATGCATCTGTTCAGTACATCCTAGACTCAGTGATTTACTCTCTACTGAACGATCCCACCCGTCGCTTCAT ATACGTGGAAATGGCTTTCTTCTCCCGTTGGTGGAAGCAGCAGACCAATGTAACACAGGACGCTGTACGG AACCTGGTGCGCCAGGGGCGCCTGGAGTTTGTCAATGGTGGCTGGGTAATGAATGATGAGGCAGCCACTC ACTATGGTGCTATTGTGGACCAGATGACACTTGGGCTACGCTTCCTGCAGGACACATTTGGTAGTGATGG ACTTCCCCGTGTGGCCTGGCACATTGACCCTTTTGGCCATTCTCGAGAACAGGCCTCCCTGTTTGCCCAG ATGGGTTTTGATGGCTTCTTCCTCGGGCGCATTGACTACCAAGATAAATTTAACCGGAAGAGAAAGCTGA AGATGGAGGAACTCTGGAGGGCTAGCGCCAGCTTGAAGCCCCCTGCTGCTGACCTCTTTACTGGTGTGCT CCCCAATAATTACAACCCTCCAAAAGACCTGTGCTGGGATGTGCTGTGTACAGACCCTCCCGTGGTGGAC GATCCCACAAGCCCTGAGTTTAATGCCAATAAGTTGGTTGATTACTTCCTGAACCTCGCCTCTTCCCAGA AAAAGTATTACCGAACCAACCACACCGTGATGACCATGGGCTCAGACTTCCAGTATGAGAATGCCAACAT GTGGTTCAAAAACATGGACAAGCTTATCCGCCTGGTCAACGAGCAGCAGGCAAACGGGAGCAAGGTCCAC GTGCTCTACTCCACCCCTTCGTGTTACCTCTGGGAGCTGAACAAGGCCAATCTCACTTGGACAGTGAAGG AGGACGACTTCTTTCCTTATGCTGATGGCCCCCACATGTTCTGGACTGGCTATTTCTCCAGTAGGCCAGC CCTCAAGCGCTATGAGCGCCTCAGCTACAACTTCCTGCAGGTGTGCAACCAGCTGGAAGCACTGGTGGGT CCCGAGGCCAAAGTGGGACCCTACGGCTCAGGAGACAGTGCACCCCTCAATGAGGCAATGGCGGTGCTGC AGCACCACGATGCTGTCACTGGTACTGCAAGGCAGAATGTTGTCAATGACTATGCGAAGCAGCTGGCGGC AGGCTGGGGACCCTGTGAGGTTCTCGTGAGCAACGCTCTGGCGCGACTCAGCCTTTACAAACAGAACTTC TCATTCTGTCGGGAGATCAACATCAGTATCTGCCCAGCCAGCCAGACGTCAGAGCACTTCCGGGTAATTA TTTACAACCCCGTGGGGCGGAAGGTGGATCTGATGGTTCGGCTGCCGGTCAGTGAAGGCATCTTCCTTGT GAAGGACCCTAACGATAGGAGAATTTCCAGTAATGTGGTGATGGTTCCCAGTGCTTACAGTAAGACGTAT CAATGGGAGCTGCTGTTCCCAGCCTCAGTGCCTGCCCTGGGCTTCAGCATCTACTCTGTGAACAAAATGA GTGGCCATAACCATCAGGCCCACAACCTAACGGCCAGGCCCAAGAAATCCAAGTCCCGTGTCTTAGTCAT TGAAAATAAGTACATACGGGCCACATTTGACTCTGACACAGGGTTATTGAGGAAGATTGAAAACCTGGAA CAGAACATTTCGCTCCCTGTGAGACAAGGCTTCTTTTGGTACAATGCCAGCGCTGGTGATGAGGAGAGTC CTCAGGCCTCTGGTGCCTACATCTTCAGACCCAGTCATCGTAAACCACTGCCTGTGAGCCACTGGGCTCA GGTCACTTTGGTGAAGACAAACTTGGTGCAGGAGGTACACCAGAATTTTTCAGCCTGGTGTTCCCAAGTG ATTCGCCTGTATGAAGGACAGCGTCATTTGGAGCTGGAGTGGACGGTGGGACCAATCCCAGTGAAAGATG ACTGGGGAAAGGAAGTCATCAGCCGCTTTAACACCCCGATGAGAACCAGGGGGCAGTTCTTCACGGACAG CAACGGCCGGGAAATCCTGAAGAGGAGGGACGATTTCCGACCCACCTGGACATTAAATCAGACTGAACCA GTGGCTGGAAATTACTACCCTGTCAACACTCGCATTTACATCACGGACGGGCATATGCAGCTGACCGTGC TGACCGACCGTTCCCAGGGGGGCAGCAGTCTGCTGGATGGCTCATTGGAGCTCATGGTTCACCGACGGCT GTTGGTAGATGATGAACGTGGGGTGGCGGAACCCTTGCTGGAGACAGATACGGGGGATAAGGTTCGAGGG CGCCACCTTGTGATTTTGAGTTCTGTCAGTGATGCGGCTGCAAGACACCGACTGCTGGCGGAGCAGGAAG TCCTGGCCCCTCAGGTGGTGCTGGCCCACGGGGGTAGCAGTCCCTATCACTCGCAGGCACCAAAAATGCA GTTCTCCGCACTTCGCCGGGAGCTACCTCCGCAGGTGCATCTGCTCACCCTGGCTCGCTGGGGTCCAAAG ATGCTGCTGCTGCGCTTGGAGCACCAGTTCGCTGTGAAGGAAGATTCAAATCGCAACCTGAGCTCTCCGG TGACCTTGAACTTGCAGAACCTGTTCAAGACCTTCACTATCAACTACCTGCAGGAGACCACACTGGCAGC CAACCAGCCCCTATCCAGGGTTTCCAGGCTCAAGTGGATGACAGATACTGGTCCCATCTCCTACCCTGCT CCTTCCCGGTTGGACCCTACCTCAATCACACTGCAGCCTATGCAAATCCGTACCTTCCTGGCCTCGGTTC AATGGCAGAGACTCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204232 representing NM_199404
Red=Cloning site Green=Tags(s) MGTGSLAPGVRAGGGNTGWLWMSSCNLGLPVLSISFLIWLLLAAPGAQAAGYKTCPTTKPGMLNVHLLPH THDDVGWLKTVDQYYYGIMSDVQHASVQYILDSVIYSLLNDPTRRFIYVEMAFFSRWWKQQTNVTQDAVR NLVRQGRLEFVNGGWVMNDEAATHYGAIVDQMTLGLRFLQDTFGSDGLPRVAWHIDPFGHSREQASLFAQ MGFDGFFLGRIDYQDKFNRKRKLKMEELWRASASLKPPAADLFTGVLPNNYNPPKDLCWDVLCTDPPVVD DPTSPEFNANKLVDYFLNLASSQKKYYRTNHTVMTMGSDFQYENANMWFKNMDKLIRLVNEQQANGSKVH VLYSTPSCYLWELNKANLTWTVKEDDFFPYADGPHMFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVG PEAKVGPYGSGDSAPLNEAMAVLQHHDAVTGTARQNVVNDYAKQLAAGWGPCEVLVSNALARLSLYKQNF SFCREINISICPASQTSEHFRVIIYNPVGRKVDLMVRLPVSEGIFLVKDPNDRRISSNVVMVPSAYSKTY QWELLFPASVPALGFSIYSVNKMSGHNHQAHNLTARPKKSKSRVLVIENKYIRATFDSDTGLLRKIENLE QNISLPVRQGFFWYNASAGDEESPQASGAYIFRPSHRKPLPVSHWAQVTLVKTNLVQEVHQNFSAWCSQV IRLYEGQRHLELEWTVGPIPVKDDWGKEVISRFNTPMRTRGQFFTDSNGREILKRRDDFRPTWTLNQTEP VAGNYYPVNTRIYITDGHMQLTVLTDRSQGGSSLLDGSLELMVHRRLLVDDERGVAEPLLETDTGDKVRG RHLVILSSVSDAAARHRLLAEQEVLAPQVVLAHGGSSPYHSQAPKMQFSALRRELPPQVHLLTLARWGPK MLLLRLEHQFAVKEDSNRNLSSPVTLNLQNLFKTFTINYLQETTLAANQPLSRVSRLKWMTDTGPISYPA PSRLDPTSITLQPMQIRTFLASVQWQRLI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_199404 |
| ORF Size | 3027 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_199404.1, NP_955436.1 |
| RefSeq Size | 3150 bp |
| RefSeq ORF | 3030 bp |
| Locus ID | 361378 |
| MW | 114.3 kDa |
| Gene Summary | mouse homolog is a lysosomal alpha-mannosidase which cleaves alpha-linked mannose residues on glycoproteins [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN204232 | Man2b1 (untagged ORF) - Rat mannosidase, alpha, class 2B, member 1 (Man2b1), (10 ug) |
CNY 16,250.00 |
|
| RR204232L3 | Lenti ORF clone of Man2b1 (Myc-DDK-tagged ORF) - Rat mannosidase, alpha, class 2B, member 1 (Man2b1), (10 ug) |
CNY 17,770.00 |
|
| RR204232L4 | Lenti ORF clone of Man2b1 (mGFP-tagged ORF) - Rat mannosidase, alpha, class 2B, member 1 (Man2b1), (10 ug) |
CNY 19,570.00 |
