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Top1mt (NM_001002798) Rat Tagged ORF Clone
CAT＃: RR204378
- TrueORF®
Top1mt (Myc-DDK-tagged ORF) - Rat topoisomerase (DNA) I, mitochondrial (Top1mt), nuclear gene encoding mitochondrial protein, (10 ug)
"NM_001002798" in other vectors (3)
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CNY 7,510.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR204378 representing NM_001002798
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTGCTATGGCTCAGAGCTCTGTGCCGGCGTTTCCAGCATGTCCCCCGCCGCGTCCCCAGCCGCC AAGTGTCCCGGGGCTCGAAGGCGAGCAGAGCCGGGTGGGGAGAGACGAGCAAGAGCAGTGTGAAGTGGAA GCAACTGGAGCACAAGGGTCCGTGCTTCGCCCCAGCCTATGAGCCCCTTCCTGATGGAGTACGTTTCTTC TATGATGGCAAGCCAGTGAGGCTGAGCTTGGCAGCGGAGGAGGTTGCTACTTTCTATGGGAAAATGCTGC ATCTCGAGTGTACGACCAAGGAAGTCTTCCGGAGGAACTTCTTCAGCGACTGGCAGAAGGAAATGACAGC TGAGGAGAGGAAGCTCATCACACACCTGGACAAGTGCGACTTCTCGGAGATCCATAGGCACTTCATGGAA AGAGCTGAGGCCCGCAGGACCCTTCCCAGGGAGCAGAAGCAGAAACTAAAAGAAGAGGCAGAGAAGCTTC AGCAGGAGTTTGGCTACTGTATTTTAGATGGCCACAGAGAGAAAATAGGCAATTTCAAGACGGAACCACC CGGCTTGTTCCGCGGCCGAGGCGACCATCCCAAGATGGGGATGTTGAAGAGGAGGGTCATGCCAGAGGAT GTGGTCATTAACTGTAGCAGAGATTCCAAGATCCCAGAGCCCCCGGCTGGTCATCAGTGGAAAGAGGTCC GCTCGGATAACACAGTCATGTGGCTGGCTGCCTGGGTGGAGAACATCCAGAACTCCTTCAAGTACATCAT ACTGAACCCCAGCTCCAAGCCGAAGGGAGAGATGGATTGGCAGAAGTATGAGGTAGCACGACGCTTGAAG GGGGTTGTGGACAAGATCCGTGCTCAGTATCAGGCTGACTGGAAGTCACCAGAAATGAAGAAAAGGCAGC TGGCTGTAGCCCTTTATTTTATTGATAAGCTGGCACTGCGAACAGGAAATGAGAAGGAAGAGGGTGAGAC GGCCGACACCGTGGGCTGCTGTTCGCTCCGAGTGGAGCACGTCCGACTGCACACGCCCGCAGATGGCCAG GAGCATGTGGTAGAGCTGGACTTCTTGGGGAAGGATTCCATCCGTTATAAGAATCACGTGACGGTGGAGA AGCTTGTGTTCCAGAACCTTCAGCACTTCATGGAAGACAAGGACCCTAGGGACGACCTCTTCGATGCACT GACTACTTCCAGCCTGAACAAGCACTTGCAGGACTTGATGGAGGGGCTGACAGCCAAGGTGTTTCGGACC TACAATGCCTCCATCACTCTGCAGGAGCAGCTGCGGGTGCTGACCCGTGCTGAAGACAGCCTAACCTGCA AAGTCTTAGCTTACAACCGGGCAAACCGGGCTGTGGCGGTCCTCTGTAACCATCAGAGAGCAATTCCTAA GACCTTCGAGGAGTCCATGCAGACACTGCAGAAAAAGATTGAGACAAAGAAGGCACAGGTGGCTGAGGCA CAGGTGGAGCTGCAGAAGGCAGAGACTGACCTTAGAATGAGAGGGGACAGCAAGTCCAAAAGTTTCCTTC AGAAGCAGCAGCGGCTGCTGAAGCTGGAGGAGCAGCTGGCTCGACTGTGCACGAAGGCCACAGACAAGGA GGAGAATAAGCAAGTGGCCCTGGGCACCGCCAAGCTCAACTACCTGGACCCCCGGATCAGCATTGCATGG TGTAAGAGATTTGGTGTGCCCGTAGAGAAGATCTACAACAAGACCCAGAGAGAGAGGTTTGCTTGGGCCT TCAACCAGGCAGGTGAAGACTTTGAATTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204378 representing NM_001002798
Red=Cloning site Green=Tags(s) MLLLWLRALCRRFQHVPRRVPSRQVSRGSKASRAGWGETSKSSVKWKQLEHKGPCFAPAYEPLPDGVRFF YDGKPVRLSLAAEEVATFYGKMLHLECTTKEVFRRNFFSDWQKEMTAEERKLITHLDKCDFSEIHRHFME RAEARRTLPREQKQKLKEEAEKLQQEFGYCILDGHREKIGNFKTEPPGLFRGRGDHPKMGMLKRRVMPED VVINCSRDSKIPEPPAGHQWKEVRSDNTVMWLAAWVENIQNSFKYIILNPSSKPKGEMDWQKYEVARRLK GVVDKIRAQYQADWKSPEMKKRQLAVALYFIDKLALRTGNEKEEGETADTVGCCSLRVEHVRLHTPADGQ EHVVELDFLGKDSIRYKNHVTVEKLVFQNLQHFMEDKDPRDDLFDALTTSSLNKHLQDLMEGLTAKVFRT YNASITLQEQLRVLTRAEDSLTCKVLAYNRANRAVAVLCNHQRAIPKTFEESMQTLQKKIETKKAQVAEA QVELQKAETDLRMRGDSKSKSFLQKQQRLLKLEEQLARLCTKATDKEENKQVALGTAKLNYLDPRISIAW CKRFGVPVEKIYNKTQRERFAWAFNQAGEDFEF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001002798 |
| ORF Size | 1779 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001002798.1, NP_001002798.1 |
| RefSeq Size | 1887 bp |
| RefSeq ORF | 1782 bp |
| Locus ID | 300029 |
| UniProt ID | Q6IM78 |
| MW | 69 kDa |
| Gene Summary | Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN204378 | Top1mt (untagged ORF) - Rat topoisomerase (DNA) I, mitochondrial (Top1mt), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 7,510.00 |
|
| RR204378L3 | Lenti ORF clone of Top1mt (Myc-DDK-tagged ORF) - Rat topoisomerase (DNA) I, mitochondrial (Top1mt), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 9,500.00 |
|
| RR204378L4 | Lenti ORF clone of Top1mt (mGFP-tagged ORF) - Rat topoisomerase (DNA) I, mitochondrial (Top1mt), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 10,360.00 |
