Cacna1f (NM_053701) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR204450 representing NM_053701 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGAATCTGAAGTCGGGAAAGACACCACCCCAGAACCCAGTCCAGCCAATGGGACCGGTCCTGGCC CTGAGTGGGGGCTCTGTCCTGGGCCTCCAACCGTGGGGACTGATACCAGTGGGGCATCGGGCCTGGGGAC CCCAAGACGGAGGACTCAGCACAACAAGCACAAGACCGTAGTGGTGGCCAGTGCGCAGAGATCACCTCGA GCACTCTTCTGCCTCACCTTAACCAATCCCATTCGTCGGTCCTGCATCAGCATTGTGGAGTGGAAGCCAT TTGATATTCTCATCCTCCTGACCATCTTTGCCAACTGTGTGGCATTGGGGGTATATATCCCCTTCCCTGA GGACGACTCCAACACTGCTACCCACAACTTGGAACAGGTAGAATATGTGTTCCTGGTGATTTTCACCGTG AAGACAGTGCTCAAGATCGTGGCCTATGGGCTGGTGCTCCATCCCAGCGCCTACATTCGCAATGGTTGGA ACCTACTCGACTTCATCATCGTTGTAGTCGGGCTGTTCAGCGTGTTGCTGGAGCAGGGACCTGGGCGGCC AGGAGATGCCCCGCATACTGGAGGAAAGCCGGGAGGCTTTGATGTAAAGGCACTGCGGGCATTTAGGGTG CTACGACCACTAAGGCTAGTGTCTGGGGTCCCGAGTCTGCACATAGTGCTCAATTCCATCATGAAGGCGC TTGTGCCGCTTCTGCACATTGCCCTGTTGGTGCTCTTCGTCATTATCATTTACGCCATCATCGGACTCGA GCTGTTCCTCGGACGAATGCACAAGACGTGCTACTTCCTGGGATCCGACATGGAGGCAGAGGAGGACCCG TCGCCTTGTGCATCTTCTGGCTCTGGCCGTTCATGCACACTGAACCAGACTGAGTGCCGTGGGCGCTGGC CAGGACCCAACGGTGGCATCACGAACTTTGACAATTTTTTCTTTGCCATGCTAACTGTGTTCCAGTGTAT TACCATGGAAGGCTGGACAGACGTCCTCTACTGGATGCAGGATGCCATGGGGTATGAGCTACCTTGGGTG TACTTTGTGAGCCTCGTCATCTTTGGGTCTTTCTTTGTCCTCAACCTTGTGCTTGGAGTCCTAAGTGGGG AGTTCTCCAAGGAAAGAGAAAAGGCAAAAGCCCGAGGTGACTTTCAGAAGCTTCGGGAGAAGCAGCAGAT GGAAGAGGACCTTCGGGGCTACCTGGACTGGATCACACAGGCTGAGGAGTTGGACCTTCATGACCCCTCA GTAGACGGCAACTTGGCTTCTCTTGCTGAAGAGGGACGGGCAGGCCATCGACCACAACTGTCAGAGCTGA CCAATAGGAGGCGCGGACGACTGCGTTGGTTCAGCCACTCCACTCGCTCCACACACTCCACCAGCAGTCA TGCCAGCCTTCCAGCCAGTGACACTGGTTCCATAACAGACACCCCCGGAGATGAGGATGAAGAGGAGGGG ACCATGGCCAGCTGTACACTCTGCCTAAACAAGATTATGAAAACCAAGGTCTGCCGCCACTTCCGCCGAG CCAACCGTGGTCTCCGTGCACGCTGCCGCCGGGCAGTCAAGTCCAATGCCTGCTACTGGGCTGTATTGCT GCTCGTCTTCCTCAACACGTTGACCATCGCTTCTGAGCACCATGGGCAGCCAGTGTGGCTCACCCAGACC CAAGAATATGCCAACAAGGTTCTGCTCTGCCTCTTCACCGTGGAGATGCTTCTCAAACTGTACGGGCTGG GCCCCTCTGTCTACGTTGCCTCTTTTTTCAACCGCTTTGACTGTTTCGTGGTCTGTGGGGGCATCCTAGA AACGACGTTGGTGGAGGTGGGGGCCATGCAGCCCCTCGGCATCTCAGTGCTCCGATGTGTGCGTCTCCTC AGGATCTTCAAGGTCACCAGGCACTGGGCATCCCTCAGCAATCTGGTGGCATCGCTGCTCAACTCCATGA AGTCCATCGCCTCCTTGCTGCTTCTCCTCTTTCTCTTTATCATCATCTTCTCCCTTCTTGGCATGCAGCT GTTTGGGGGCAAGTTCAACTTTGACCAGACCCACACTAAGAGAAGCACCTTTGATACCTTCCCCCAAGCC CTCCTCACCGTCTTTCAGATCCTGACTGGTGAGGATTGGAATGTAGTCATGTATGACGGTATCATGGCCT ACGGTGGTCCCTTCTTCCCAGGGATGCTGGTGTGTGTTTATTTCATCATCCTCTTCATCTGTGGCAACTA CATCCTACTGAACGTGTTTCTTGCCATTGCTGTGGATAACCTAGCCAGCGGGGATGCGGGTGCTGCCAAA GATAAGGGCAGAGAGAAGAGCAGTGAAGGAAACCCTCCCCAAGAGAACAAAGTATTGGTGCCTGGTGGAG AAAATGAGGACACAGAGGGCACAAAAAGTGAAGGAGCAGCACCAGGCATGGAGGAAGAGGAGGAAGAAGA GGAGGAGGAGGAGGAAGAAAATGGTGCAGGACATGTGGAACTCCTGCAGGAAGTTGTACCCAAGGAGAAG GTGGTACCCATCCCTGAAGGCAGTGCCTTCTTCTGCCTCAGCCAAACCAACCCGCTTCGCAAGGCCTGCC ACACTCTCATACACCATCACGTGTTCACCAGTCTCATCCTGGTGTTCATCATCCTCAGTAGTGTGTCCCT GGCTGCTGAGGACCCCATCAGAGCCCACTCTTTCCGCAACCATATTCTGGGGTACTTCGATTATGCCTTC ACTTCCATTTTCACCGTGGAGATTCTATTAAAGATGACGGTGTTTGGGGCTTTCCTGCACCAAGGTTCTT TCTGCCGTAGCTGGTTCAATCTGTTGGATCTGCTTGTGGTCAGTGTGTCCCTCATCTCCTTTGGCATCCA CTCCAGTGCCATCTCAGTTGTGAAGATTCTCCGAGTCCTCCGAGTCCTGCGGCCTCTCCGAGCCATTAAC CGAGCCAAGGGACTCAAGCATGTGGTGCAGTGTGTGTTTGTGGCCATCCGGACCATTGGAAACATCATGA TTGTCACCACCCTCTTGCAGTTCATGTTCGCCTGCATTGGTGTTCAGCTGTTCAAGGGAAAATTCTACAG TTGCACTGATGAAGCCAAACACACCCTGAAAGAATGCAAGGGCTCCTTCCTCATCTACCCTGATGGAGAT GTGTCACGACCTTTGGTCCGGGAACGCCTCTGGGTCAACAGTGATTTCAACTTTGACAACGTCCTTTCAG CCATGATGGCCCTGTTCACTGTCTCTACCTTTGAAGGCTGGCCTGCGCTACTATACAAAGCCATAGATGC ACACGCTGAAGACGAGGGCCCTATCTACAATTACCATGTGGAGATATCAGTATTCTTCATTGTCTACATC ATCATCATCGCCTTCTTCATGATGAACATCTTTGTGGGATTTGTCATCATCACATTCCGTGCCCAGGGAG AGCAGGAATATCAAAACTGTGAACTGGACAAGAACCAGCGCCAGTGTGTGGAATATGCACTCAAAGCTCA GCCACTCCGCCGATACATCCCCAAGAATCCACATCAGTACCGCGTGTGGGCCACAGTGAACTCAGCCGCC TTTGAGTACCTCATGTTTCTGCTCATCCTCCTCAACACAGTTGCCCTGGCCATGCAGCACTATGAGCAGA CGGCTCCCTTTAACTATGCCATGGACATACTCAACATGGTCTTCACTGGCCTCTTCACCGTTGAGATGGT GCTCAAAATCATTGCCTTTAAACCCAAGCATTACTTTGCCGATGCCTGGAATACATTCGATGCTCTTATT GTAGTGGGCAGCGTAGTCGACATTGCCGTCACAGAAGTCAATAATGGAGGCCATCTTGGTGAGAGCTCAG AGGACAGCTCCCGCATATCTATCACGTTCTTTCGCCTCTTCCGGGTCATGAGGCTGGTGAAGCTGCTCAG TAAGGGTGAGGGGATCCGCACACTGCTCTGGACATTCATCAAGTCTTTCCAGGCCTTGCCCCATGTGGCA CTTCTTATAGCAATGATATTCTTCATATATGCAGTCATTGGCATGCAGATGTTTGGCAAAGTGGCTCTTC AGGACGGCACACAGATAAATCGAAACAATAATTTCCAGACCTTTCCGCAGGCTGTGCTGCTTCTGTTCAG GTGTGCCACTGGTGAGGCATGGCAAGAGATAATGCTAGCCAGCCTTCCAGGAAATCGGTGTGACCCTGAG TCTGACTTTGGCCCAGGCGAGGAATTTACCTGTGGTAGCAATTTTGCCATTGTCTACTTTATCAGCTTCT TCATGCTCTGTGCCTTCCTGATTATAAATCTCTTTGTGGCTGTAATCATGGATAACTTTGATTACCTAAC CAGAGATTGGTCTATTCTGGGACCCCACCACCTCGATGAATTCAAGAGGATCTGGTCTGAATATGACCCC GGAGCCAAGGGCCGCATCAAGCACTTGGATGTGGTTGCCCTGCTGAGACGCATCCAGCCTCCACTGGGAT TTGGAAAGCTGTGCCCACACCGAGTGGCCTGCAAGAGACTGGTGGCCATGAATGTTCCCCTCAACTCAGA CGGAACAGTGACATTCAACGCGACACTCTTTGCCCTGGTGCGGACATCCCTGAGGATCAAGACAGAAGGG AACCTGGATCAAGCCAACCAGGAGCTTCGGATGGTCATCAAAAAGATCTGGAAGCGGATAAAGCAGAAAT TGTTAGATGAGGTCATCCCCCCTCCAGATGAGGAGGAGGTCACTGTGGGAAAATTCTATGCCACATTTCT CATCCAAGATTATTTCCGAAAATTCCGGAGAAGGAAAGAAAAGGGGCTACTAGGAGCAGATGCCCCAACA AGCACATCCTCTGTCCTCCAGGCTGGTCTAAGGAGCCTACAGGACTTGGGTCCTGAGATCCGACAGGCCC TCACCTATGCCCTTGAGGAAGAAGAAGAAGAAGAAGAGGCCCGCCAGGTCAAGGAAGCTGAGGAGGAGGA AGCTGAGACCACCCCAGAAACATACAAAGACTCCATAGACTCCCAGCCCCAAGCTCAATGGAACTCGAGG ATTTCGGTGTCTCTACCCAAGTTAAGAGAAACTCCAGATTCTCTTTCAACTGGGCCTAGTGATGATGATG GAGTGGCTCCCAACTCCAGGCAACCCAGTGGGCTACAGGCTGGATCCCAACCCCACAGGAGAGGCTCTGG GGTTTTCATGTTCACTATCCCAGAAGAAGGAAGTACTCAGCTCAAGGGAGTTCAAGGGCAAGACAATCAG AATGAGGAACAGGAAGTCCCTGACTGGACCCCCAACCTGGATGAGCAGGCAGGGATGCCCTCCAACCCAG TCCTTTTACCACCTCACTGGTCCCAGCAACATGTAAATGGGCACCATGTACCACGCCGACGTTTGCTACC CCCCACGCCTGCAGGTCGGAAGCCCTCCTTCACCATCCAGTGTCTGCAACGCCAGGGCAGTTGTGAGGAT TTGCCCATCCCAGGCACCTACCATCGTGGACGGACCTCAGGACCAGGCAGGGCTCAGGGTTCCTGGGCAG CCCCTCCTCAGAAGGGTCGACTGCTCTATGCCCCCCTGTTGCTGGTGGAGGAATCTACAGTGGGTGAAGG ATACCTTGGCAAGCTCGGCGGCCCACTGCGTACCTTCACTTGTCTGCAAGTGCCTGGAGCTCCTTCAGAT CCCAGTCACCGCAAGAGGGGCAGTGCTGACAGTTTGGTGGAGGCTGTGCTTATCTCCGAAGGCCTAGGCC TCTTTGCCCAAGACCCACGATTTGTGGCCCTGGCCAAGCAAGAGATTGCAGATGCATGTCACCTGACCCT GGATGAGATGGACAGTGCTGCCAGTGACCTGCTGGCACAGAGAACCACCTCCCTCTACAGTGACGAGGAG TCTATTCTCTCCCGCTTTGATGAAGAGGACCTGGGAGACGAGATGGCCTGCGTCCATGCCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR204450 representing NM_053701 Red=Cloning site Green=Tags(s) MSESEVGKDTTPEPSPANGTGPGPEWGLCPGPPTVGTDTSGASGLGTPRRRTQHNKHKTVVVASAQRSPR ALFCLTLTNPIRRSCISIVEWKPFDILILLTIFANCVALGVYIPFPEDDSNTATHNLEQVEYVFLVIFTV KTVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLLEQGPGRPGDAPHTGGKPGGFDVKALRAFRV LRPLRLVSGVPSLHIVLNSIMKALVPLLHIALLVLFVIIIYAIIGLELFLGRMHKTCYFLGSDMEAEEDP SPCASSGSGRSCTLNQTECRGRWPGPNGGITNFDNFFFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWV YFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQMEEDLRGYLDWITQAEELDLHDPS VDGNLASLAEEGRAGHRPQLSELTNRRRGRLRWFSHSTRSTHSTSSHASLPASDTGSITDTPGDEDEEEG TMASCTLCLNKIMKTKVCRHFRRANRGLRARCRRAVKSNACYWAVLLLVFLNTLTIASEHHGQPVWLTQT QEYANKVLLCLFTVEMLLKLYGLGPSVYVASFFNRFDCFVVCGGILETTLVEVGAMQPLGISVLRCVRLL RIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDQTHTKRSTFDTFPQA LLTVFQILTGEDWNVVMYDGIMAYGGPFFPGMLVCVYFIILFICGNYILLNVFLAIAVDNLASGDAGAAK DKGREKSSEGNPPQENKVLVPGGENEDTEGTKSEGAAPGMEEEEEEEEEEEEENGAGHVELLQEVVPKEK VVPIPEGSAFFCLSQTNPLRKACHTLIHHHVFTSLILVFIILSSVSLAAEDPIRAHSFRNHILGYFDYAF TSIFTVEILLKMTVFGAFLHQGSFCRSWFNLLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAIN RAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYSCTDEAKHTLKECKGSFLIYPDGD VSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDAHAEDEGPIYNYHVEISVFFIVYI IIIAFFMMNIFVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSAA FEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTVEMVLKIIAFKPKHYFADAWNTFDALI VVGSVVDIAVTEVNNGGHLGESSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPHVA LLIAMIFFIYAVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGNRCDPE SDFGPGEEFTCGSNFAIVYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDP GAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNVPLNSDGTVTFNATLFALVRTSLRIKTEG NLDQANQELRMVIKKIWKRIKQKLLDEVIPPPDEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGADAPT STSSVLQAGLRSLQDLGPEIRQALTYALEEEEEEEEARQVKEAEEEEAETTPETYKDSIDSQPQAQWNSR ISVSLPKLRETPDSLSTGPSDDDGVAPNSRQPSGLQAGSQPHRRGSGVFMFTIPEEGSTQLKGVQGQDNQ NEEQEVPDWTPNLDEQAGMPSNPVLLPPHWSQQHVNGHHVPRRRLLPPTPAGRKPSFTIQCLQRQGSCED LPIPGTYHRGRTSGPGRAQGSWAAPPQKGRLLYAPLLLVEESTVGEGYLGKLGGPLRTFTCLQVPGAPSD PSHRKRGSADSLVEAVLISEGLGLFAQDPRFVALAKQEIADACHLTLDEMDSAASDLLAQRTTSLYSDEE SILSRFDEEDLGDEMACVHAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053701
ORF Size	5943 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053701.1, NP_446153.1
RefSeq Size	5975 bp
RefSeq ORF	5946 bp
Locus ID	114493
MW	221.1 kDa
Gene Summary	mutation of the human homolog causes incomplete X-linked congentital stationary night blindness (CSNB2) [RGD, Feb 2006]

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