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Arhgef2 (NM_001012079) Rat Tagged ORF Clone
CAT＃: RR205579
- TrueORF®
Arhgef2 (Myc-DDK-tagged ORF) - Rat rho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2), (10 ug)
"NM_001012079" in other vectors (3)
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CNY 12,640.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC95068 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR205579 representing NM_001012079
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCGGATCGAATCCCTCACTCGCGCGCGGATCGACCGGAGCAAGGAGCAGGCGACCAAGACCCGGG AAAAGGAGAAGATGAAGGAAGCTAAAGATGCCCGCTATACCAACGGCCACCTCTTCACCACCATCTCGGT CTCCGGCATGACTATGTGCTATGCCTGTAACAAGAGCATTACAGCCAAGGAAGCCCTCATTTGTCCCACA TGTAACGTGACCATCCACAACCGCTGTAAGGACACGCTGGCCAACTGTACCAAGGTCAAGCAGAAGCAAC AGAAAGCTGCACTGCTGAGGAACAACACTGCCTTGCAGTCCGTCTCTCTCCGAAGTAAGACAACCACCAG AGAGCGGCCAACGTCTGCCATCTACCCTTCCGATAGCTTCCGGCAGTCCCTCCTGGGTTCTCGTCGTGGC CTGTCCTCCTTGTCTTTGGCCAAAAGTGTTTCCACTACCAACATTGCTGGACATTTCAATGATGAGTCTC CTCTGGGGCTGCGTCAGATCCTCTCCCAGTCCACAGACTCCCTCAACATGCGGAACCGAACCCTGTCCGT GGAGTCCCTTATTGATGAAGGTGTAGAAGTGTTCTACAACGAGCTCATGAGTGACTTTGAGATGGACGAG AAGGACTTCGAGGCGGATTCATGGAGCCTTGCTGTGGACAGCAGCTTCCTGCAACAGCATAAAAAGGAGG TGATGAAGAAGCAAGATGTCATCTACGAGCTGATCCAGACCGAGCTGCACCATGTGAGAACCTTGAAGAT TATGACCCGCCTCTTCCGCACTGGGATGCTGGAAGAGTTGCAGATGGAGCCAGAAGTGGTCCAGGGACTG TTCCCCTGTGTGGACGAGCTTAGTGACATTCACACACGTTTCCTTAGTCAGCTTTTGGAACGGCGGCGCC AGGCCCTGTGTCCAGGCAGCACCCGGAACTTTGTCATCCATCGTTTGGGTGACTTGCTCATCAGCCAGTT CTCAGGTTCCAACGCTGAGCAGATGCGCAAGACTTACTCAGAGTTCTGCAGCCGCCACACCAAGGCCTTA AAGCTCTATAAGGAGCTGTACGCTCGAGACAAACGCTTCCAACAGTTCATCCGGAAAATGACCCGCTCTG CTGTGCTGAAGCGGCATGGGGTTCAGGAGTGCATTCTCCTGGTAACTCAGCGGATTACCAAATACCCAGT GCTCATCAACAGGATCCTGCAGAATTCCCATGGGATTGAAGAGGAGTACCAAGACTTGGCAGCAGCCCTA GGGCTAGTAAAGGAGTTGTTGTCCAATGTGGACCAGGATGTACACGAGCTGGAGAAAGAGGCCCGCCTTC AGGAGATTTACAACCGAATGGATCCTCGGGCTCAGACCCCTGTTCCTGGCAAGGGCCCCTTCGGCCGAGA TGAACTTTTGCGGAGAAAACTTATCCATGATGGCTGCCTGCTCTGGAAGACAGCCACAGGCCGCTTTAAA GATGTCCTCTTACTACTGATGACAGATGTGCTGGTGTTTCTCCAGGAAAAGGACCAGAAGTACATTTTCA CGTCCCTGGACAAGCCCTCAGTGGTATCCTTGCAGAATCTCATCGTAAGAGACATTGCCAACCAGGCGAA AGGGATGTTTCTGATCAGTTCTGGCCCACCTGAAATGTATGAAGTACACGCAGCATCTCGAGATGACCGT ACTACCTGGATCCGTGTCATCCAGCAGAGCGTGCGCTTATGCCCGTCGAGGGAGGACTTCCCTCTGATCG AGACAGAGGATAAGGCGTACCTCCGGAGGATCAAGACGAAACTGCAGCAGAAAAACCAGGCGCTGGTGGA GCTGCTACAGATGAATGTTGAGCTATTTGCTGAGATGGTCCACTTCCAGGCATTAAAAGCTGGTTTCATC GGAATGCCCCCGCCCACCCTGCCCAGGGGCCTTTTCCGTCTTGAGTCCTTTGAGTCCCTTCGAGGCGAGC GTCTGCTCAAAGATGCCCTCCGTGAAGTGGAAGGCCTGAAAGACCTGTTGTTGGGCCCTTGTGTGGACCT GCCTTTGACAGCCCGAGAACCAGCCTTACCCGTAGAAGCTGACAGTGGTAGCTGTCCTGGGGTCACTGCC AACGGAGAGGCCAGAACCTTCAATGGCTCCATTGAGCTCTGTAGAGCAGACTCAGATTCCAGCCAGAAGG ATCGGAATGGAAATCAGTTGAGGTCACCACAGGAGGAGGCGTTACAACCATTGGTCAATCTGTATGGACT CCTACAAGGCCTGCAGGCTGTTGTGGTCCAGCAAGAAAGATTGATGGAAGCCCTGTTCCCTGAAGGCCCT GAGCGGTGGGAAAAGCTATCCCGAGCCAACTCTCGGGATGGCGAAGCTGGCCGGGCTGCGGTTGCTTCTG TAACTCCCGAGAAGCAGGCCACGGAGCTGGCATTACTGCAGAGGCAGCACAGCCTGTTGCAGGAAGAGCT GCGGCGCTGCCAGCGGCTCGGGGAAGAGCGGGCAACTGAAGCTGGCAGCCTGGAGGCCAGGCTGCGGGAG AGCGAGCAAGCCCGGGCCCTGCTGGAGCGGGAGGCTGAAGAGATCCGCCGGCAGCTAGCAGCCTTGGGCC AAAACGAGCCACTCCCGGCAGAAGCACCCTGGGCTCGCAGGCCTCTGGACCCTCGGCGCCGCAGCCTTCC AGCAGGCGACGCTCTGTACTTGAGCTTCAATCCCCCCCAGCCTAGTCGAGGCCATGACCGCCTGGATTTG CCTGTGACTGTTCGTTCCCTCCACCGACCCTTTGATGACCGAGAGGCACAAGAACTTGGTAGCCCCGAGG ATCGACTGCAGGACAGCAGTGACCCTGATACTTGCAGTGAGGAGGAAGTCAGTAGCCGGCTGTCCCCACC CCACAGTCCACGAGACTTCACCCGAATGCAGGACATTCCCGAAGAGACAGAAAGCCGAGATGGGGAGCCT ACGGCCTCAGAGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR205579 representing NM_001012079
Red=Cloning site Green=Tags(s) MSRIESLTRARIDRSKEQATKTREKEKMKEAKDARYTNGHLFTTISVSGMTMCYACNKSITAKEALICPT CNVTIHNRCKDTLANCTKVKQKQQKAALLRNNTALQSVSLRSKTTTRERPTSAIYPSDSFRQSLLGSRRG LSSLSLAKSVSTTNIAGHFNDESPLGLRQILSQSTDSLNMRNRTLSVESLIDEGVEVFYNELMSDFEMDE KDFEADSWSLAVDSSFLQQHKKEVMKKQDVIYELIQTELHHVRTLKIMTRLFRTGMLEELQMEPEVVQGL FPCVDELSDIHTRFLSQLLERRRQALCPGSTRNFVIHRLGDLLISQFSGSNAEQMRKTYSEFCSRHTKAL KLYKELYARDKRFQQFIRKMTRSAVLKRHGVQECILLVTQRITKYPVLINRILQNSHGIEEEYQDLAAAL GLVKELLSNVDQDVHELEKEARLQEIYNRMDPRAQTPVPGKGPFGRDELLRRKLIHDGCLLWKTATGRFK DVLLLLMTDVLVFLQEKDQKYIFTSLDKPSVVSLQNLIVRDIANQAKGMFLISSGPPEMYEVHAASRDDR TTWIRVIQQSVRLCPSREDFPLIETEDKAYLRRIKTKLQQKNQALVELLQMNVELFAEMVHFQALKAGFI GMPPPTLPRGLFRLESFESLRGERLLKDALREVEGLKDLLLGPCVDLPLTAREPALPVEADSGSCPGVTA NGEARTFNGSIELCRADSDSSQKDRNGNQLRSPQEEALQPLVNLYGLLQGLQAVVVQQERLMEALFPEGP ERWEKLSRANSRDGEAGRAAVASVTPEKQATELALLQRQHSLLQEELRRCQRLGEERATEAGSLEARLRE SEQARALLEREAEEIRRQLAALGQNEPLPAEAPWARRPLDPRRRSLPAGDALYLSFNPPQPSRGHDRLDL PVTVRSLHRPFDDREAQELGSPEDRLQDSSDPDTCSEEEVSSRLSPPHSPRDFTRMQDIPEETESRDGEP TASES TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001012079 |
| ORF Size | 2955 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001012079.1, NP_001012079.1 |
| RefSeq Size | 4217 bp |
| RefSeq ORF | 2958 bp |
| Locus ID | 310635 |
| UniProt ID | Q5FVC2 |
| MW | 111.9 kDa |
| Gene Summary | Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Involved in innate immune signaling transduction pathway promoting cytokine IL6/interleukin-6 and TNF-alpha secretion in macrophage upon stimulation by bacterial peptidoglycans; acts as a signaling intermediate between NOD2 receptor and RIPK2 kinase. Contributes to the tyrosine phosphorylation of RIPK2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Overexpression activates Rho-, but not Rac-GTPases, and increases paracellular permeability. Involved in neuronal progenitor cell division and differentiation. Involved in the migration of precerebellar neurons.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN205579 | Arhgef2 (untagged ORF) - Rat rho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2), (10 ug) |
CNY 12,640.00 |
|
| RR205579L3 | Lenti ORF clone of Arhgef2 (Myc-DDK-tagged ORF) - Rat rho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2), (10 ug) |
CNY 14,350.00 |
|
| RR205579L4 | Lenti ORF clone of Arhgef2 (mGFP-tagged ORF) - Rat rho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2), (10 ug) |
CNY 15,770.00 |
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