Kif13a (NM_001107462) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR207278 representing NM_001107462 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGAGTCGAACACGACAAAATATGCCGGTCAAGAAGTCGTTTTCAAGTGCCTCGGGGAAGGGATTC TTGAGAAAGCCTTTCAGGGATACAATGCCTGTATTTTTGCATATGGCCAGACAGGCTCTGGAAAATCCTT TTCCATGATGGGCCATGCCGAGCAGCTAGGCCTGATCCCAAGGCTCTGCTGTGCTCTGTTTCAAAGGATC TCCCTGGAGCAAAACGAGTCACAAACATTTAAAGTCGAAGTATCGTATATGGAGATTTATAATGAGAAGG TCCGGGACCTCCTGGACCCCAAAGGGAGCAGGCAGTCTCTTAAAGTCCGAGAACATAAGGTCTTAGGACC CTATGTCGATGGCCTATCTCAACTGGCGGTCACCAGCTTTGAGGACATTGAGTCACTGATGTCTGAGGGA AATAAGTCTCGAACGGTAGCAGCAACCAACATGAATGAAGAGAGCAGCCGCTCCCATGCGGTGTTCAACA TCATCATCACACAGACACTGTATGACCTACAGTCTGGGAACTCTGGTGAGAAAGTCAGCAAGGTGAGCCT GGTAGACCTGGCGGGCAGCGAGAGGGTGTCGAAGACTGGAGCTGCGGGGGAGCGACTGAAAGAAGGCAGC AACATTAACAAATCACTTACAACCTTGGGATTGGTTATATCATCGCTGGCTGACCAGGCTGCTGGCAAAG GCAAAAACAAATTCGTACCTTATCGAGATTCAGTCCTCACTTGGCTTCTTAAGGACAATTTAGGTGGGAA CAGCCAGACCTCTATGATAGCAACCATCAGCCCAGCAGCAGACAACTACGAGGAGACACTGTCCACGCTA AGATACGCAGACCGAGCCAAGAGGATTGTGAACCACGCTGTCGTGAACGAGGACCCCAATGCCAAGGTCA TCCGAGAGCTGCGAGAGGAAGTGGAGAAACTGAGAGAGCAGCTCTCACAGGCTGAGGCCATGAAGGCCCC CGAGCTGAAGGAGAAGCTGGAAGAGTCTGAGAAGCTGATAAAGGAGCTCACAGTGACGTGGGAAGAGAAG CTGAGGAAAACGGAAGAGATCGCACAGGAGAGGCAGCGGCAGCTGGAGAGCATGGGGATCTCCCTGGAGA CCTCTGGCATCAAGGTGGGGGATGACAAGTGCTACCTCGTCAACCTGAATGCAGACCCTGCCCTCAACGA GCTTCTGGTTTACTATTTAAAGGATCACACGCGGGTGGGTGCTGATACCTCTCAGGACATCCAGCTCTTT GGAATAGGGATTCAGCCCGAGCACTGTGAGATCGACATCGCAACGGATGGAGATATCACTCTCACGCCCA AAGAAAACGCAAGGTCCTGTGTAAATGGCACCCTGGTCTGCAGCGTGACCCAGCTGTGGCATGGTGACAG GATCTTATGGGGAAACAACCACTTTTTCAGAATTAACTTGCCTAAGAGGAAACGGCGGGACTGGCTGAAA GACTTTGAGAAAGAAACCAGCGCCACGGAGCATGATCTAGACGCTGCGAGTGAGGCCTCCTCAGAACCAG ACTACAACTACGAGTTCGCACAGATGGAGGTCATCATGAAAACACTGAACAGCAATGATCCAGTTCAAAA TGTGGTTCAGGTCCTGGAAAAGCAGTACCTGGAAGAGAAGAGGACGGCCCTGGAGGAGCAGCGGCTCATG TATGAGCGAGAGCTAGAGCAGCTCCGTCAGCAGCTCTCTCCCGAGAGGCAGCCGCCCAGCAGCGCCTCCG ATCGCCTGGCCTACAGCAGCCAGACAGCCCAGCAGAAAGTGACCCAGTGGGCTGAGGAGAGGGATGAACT CTTTCGGCAGAGCTTGGCCAAGCTTCGAGAGCAGCTGGTGAAAGCCAACACTTTGGTGAGGGAAGCCAAC TTCCTGGCTGAGGAGATGGGCAAACTCACTGACTACCAAGTGACTCTTCAGATCCCTGCGGCCAACCTCA GTGCCAACAGGAAGAGAGGAGCGATTGTCAGTGAGCCCGCCATCCAGGCCAGGAGGAAAGGAAAGAGCAC CCAGGTCTGGACCATCGAGAAGCTGGAAAATAAGTTAATTGATATGAGAGACCTTTACCAAGAATGGAAA GAAAATGTCCCCAAGGCTAAGAGGCTCTGTGGGAAACGAGGTGACCCTTTTTATGAAGCTCAGGAGAATC ACAACCTCATCGGTGTGGCTAACGTGTTTTTGGAATGTCTCTTCTGTGATGTGAAGCTTCAATATGCAGT ACCCATTATCAGCCAGCAGGGGGAGGTTGCCGGGCGTCTCCACGTGGAGGTGATGCGTGTTACAGGAACT GTTCCAGAGCGCGTGTCGGAGGATGACTCTTCCGAGAACTCCAGTGAGAGTGGGAGTCTTGAAGTGGTGG ACAGCAGCGGGGAAGTCATCCACCGGGTCAAAAAACTGACGTGCCGGGTGATAATTAAAGAGGCAACGGG GCTACCCATAAGCCTCTCGAATTTCGTCTTCTGTCAGTACACGTTCTGGGACCAGTATGAGTCTACAGTG GCTGCCCCGGTGGTGGACCCGGACGTGCCGTCCCCTCAGTCTAAGGATGCCCAGTACACAGTGACCTTCT CTCACTGTAAGGACTACGTGGTAACTGTCACAGAAGAATTCCTAGAGTTCATTTCTGATGGCGCGCTGGC AGTTGAAGTGTGGGGCCACAGGTGTGCTGGGAACGGAAGCCCCATCTGGGAGGTCGATTCCCTCCACGCG AAGACAAGGACATTACACGACAGGTGGAATGAAGTGACCCGGAGAATAGAGATGTGGATCTCCATATTGG AACTGAATGAGCTAGGAGACTACGCCGCAGTAGAGCTTCACCAGGCGAAGGACGTCAACACCGGCGGCGT CTTCCAACTCAGACAGGGTCACTCCCGTAGAGTACAGGTCACAGTAAAACCTGTGCAGCATTCGGGGACG CTGCCACTCATGGTTGAAGCCATCCTATCAGTATCCATTGGCTGTGTGACTGCCAGATCCACCAAGCTGC AAAGAGGGCTGGACAGTTACCAGGAGGAAGACTTGAATTGTGTAAGAGAGAGATGGTCAGATGCGCTCAT TAAACGACGGGAATACTTAGACGAACAGATTAAAAAAGTCAGCAATAAAAAAGAGAAAACAGAAGATGAC ATGGAGCGGGAAGCCAGGCTGGTGGAGCAGTGGGTGGGGCTGACGGAGGAGAGGAATGCTGTGCTGGTGC CTGACCCTGGCAGCGGGATCCCGGGGGCACCTGCTGACTGGGTCCCACCTCCTGGCATGGAAACCCACAT CCCCGTTCTCTTCCTGGATCTGAACGCGGATGACCTCAGTGCCAATGAGCAGCTGGTGGGCCCCCACGCG TCGGGAGTGAACTCCATCCTGCCCAAGGAGCATGGCAGCCAGTTTTTCTACCTGCCCATCATAAAGCACA GTGATGACGAGGTTTCAGCCACAGCCTCCTGGGACTCCTCGGTGCACGACTCTCTTCACCTGAACCGGGT CACACCACAGAACGAGAGGATCTACCTGATCGTGAAGACCACCGTTCAGCTCAGCCACCCGGCCGCCATG GAGTTAGTGTTGCGGAAGCGGATTGCAGCCAATATTTATAACAAGCAGAGTTTCACGCAGAGTTTAAAGA GGCGAATATCACTGATCAATATATGTTACTCCTGCGGCGTGACCTATGAGATAGTGTCCAATATACCAAA GGCTACCGAGGAGATTGAGGACCGGGAGACGCTGGCCCTCCTGGCAGCTCGGAGTGAGAACGAGGGCACT TTGGATGGGGAGACGTACATCGAGAAGTACACTCGGGGTGTGCTGCAGGTCGAGAACATCCTGAGTCTGG AGCGGCTCCGGCAGGCCGTCACGGTGAAGGAAGCGCTCTCCACCAAAACGAGGCACATTCGGAGAAGCCT CAGTACACCGAATGTTCACAACGTCTCCTCTAGCCGGCCAGATCTCTCTGGCTTCGATGAAGATGATAAG GGCTGGCCCGAGAACCAGTTAGACGTGTCTGACTACAGCTCCAGTTACCAAGATGTCGCATGCTATGGGA CCTTACCCAGGGACTCGCCTCGCAGGAGCAAGGAAGGTTGCCCATCAGAGAATCCTCATGCCTTAACCAT CAGCCCTTTTAAAGCGTTCTCTCCTCAGCCACCAAAGTTCTTCAAACCCCTAATGCCTGTAAAAGAGGAA CACAAGAAAAGAATGGCCCTGGAAGCGAGACCTCTCCTAAGCCAAGAGGACTCCGAAGAGGAGGAAAATG AGCTGGAAGCTGTCAGCCGGAAGCTGATGCACGCACAGCCTTACGTGCCCGTGGAGTTTGCTGACTTCAG TGTTTACAATGCCAGCTTGGAGAACAGGGAGTGGTCGTCTTCTAAAGCAGACCTGACAGACTCCAGAGCC TTGGAGAAAGCAGTGTCTCGTAGCCCTACCACCAGCAGCCTCACCAGTGGCTACTTCTCCCACAGTGCCT CCAACGCCACTCTGTCTGACATGGCAGTCCCTTCCAGCGACAGCTCAGACCAGCTAGCCCTCTCCACCAA GGAGGCCGAGTGCCGGGAGCACTCGGGACCATCACTCGCACCCGACTTCACACCAGCTTCGACTCAAGAG CTTACTGAAGCAGAAAGAGGCTTGGGGAAGGATAAGACAATCACCGTGCCTCTCGAGGAGAACAGTGCCT TACCCCAGGGGAGCCCATCACCCCAGAGCATCCCCGAGGAAAATCCCAGAATGCCGTGCAGGACTGCTTT GGGTTCAGAACTGGATGCCGGCCCCAGCAAAGAGGGCCACCAAGCCAGAGAATTCTGCCCAGGAGAGGTG ACGATAGAACACACTACGAACATTTTGGAGGACCACTCCTTCACAGAGTTCATGGGTGTGTCCGATGGCA AAGACTTCGATGGTTTGGCAGATTGTTCCGTAGGAGAGCCCTCCAGTAGGACGGATCTAACCATTGAAAC AGGCCGTAAAGGTATCCCAGAGGGTCCCCCAGATGCTGGCCAGCTGCATTCCAAGATAGAGAACGACCAG ACGCTTCACCCGTTCTCTGAGATTCCA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR207278 representing NM_001107462 Red=Cloning site Green=Tags(s) MDESNTTKYAGQEVVFKCLGEGILEKAFQGYNACIFAYGQTGSGKSFSMMGHAEQLGLIPRLCCALFQRI SLEQNESQTFKVEVSYMEIYNEKVRDLLDPKGSRQSLKVREHKVLGPYVDGLSQLAVTSFEDIESLMSEG NKSRTVAATNMNEESSRSHAVFNIIITQTLYDLQSGNSGEKVSKVSLVDLAGSERVSKTGAAGERLKEGS NINKSLTTLGLVISSLADQAAGKGKNKFVPYRDSVLTWLLKDNLGGNSQTSMIATISPAADNYEETLSTL RYADRAKRIVNHAVVNEDPNAKVIRELREEVEKLREQLSQAEAMKAPELKEKLEESEKLIKELTVTWEEK LRKTEEIAQERQRQLESMGISLETSGIKVGDDKCYLVNLNADPALNELLVYYLKDHTRVGADTSQDIQLF GIGIQPEHCEIDIATDGDITLTPKENARSCVNGTLVCSVTQLWHGDRILWGNNHFFRINLPKRKRRDWLK DFEKETSATEHDLDAASEASSEPDYNYEFAQMEVIMKTLNSNDPVQNVVQVLEKQYLEEKRTALEEQRLM YERELEQLRQQLSPERQPPSSASDRLAYSSQTAQQKVTQWAEERDELFRQSLAKLREQLVKANTLVREAN FLAEEMGKLTDYQVTLQIPAANLSANRKRGAIVSEPAIQARRKGKSTQVWTIEKLENKLIDMRDLYQEWK ENVPKAKRLCGKRGDPFYEAQENHNLIGVANVFLECLFCDVKLQYAVPIISQQGEVAGRLHVEVMRVTGT VPERVSEDDSSENSSESGSLEVVDSSGEVIHRVKKLTCRVIIKEATGLPISLSNFVFCQYTFWDQYESTV AAPVVDPDVPSPQSKDAQYTVTFSHCKDYVVTVTEEFLEFISDGALAVEVWGHRCAGNGSPIWEVDSLHA KTRTLHDRWNEVTRRIEMWISILELNELGDYAAVELHQAKDVNTGGVFQLRQGHSRRVQVTVKPVQHSGT LPLMVEAILSVSIGCVTARSTKLQRGLDSYQEEDLNCVRERWSDALIKRREYLDEQIKKVSNKKEKTEDD MEREARLVEQWVGLTEERNAVLVPDPGSGIPGAPADWVPPPGMETHIPVLFLDLNADDLSANEQLVGPHA SGVNSILPKEHGSQFFYLPIIKHSDDEVSATASWDSSVHDSLHLNRVTPQNERIYLIVKTTVQLSHPAAM ELVLRKRIAANIYNKQSFTQSLKRRISLINICYSCGVTYEIVSNIPKATEEIEDRETLALLAARSENEGT LDGETYIEKYTRGVLQVENILSLERLRQAVTVKEALSTKTRHIRRSLSTPNVHNVSSSRPDLSGFDEDDK GWPENQLDVSDYSSSYQDVACYGTLPRDSPRRSKEGCPSENPHALTISPFKAFSPQPPKFFKPLMPVKEE HKKRMALEARPLLSQEDSEEEENELEAVSRKLMHAQPYVPVEFADFSVYNASLENREWSSSKADLTDSRA LEKAVSRSPTTSSLTSGYFSHSASNATLSDMAVPSSDSSDQLALSTKEAECREHSGPSLAPDFTPASTQE LTEAERGLGKDKTITVPLEENSALPQGSPSPQSIPEENPRMPCRTALGSELDAGPSKEGHQAREFCPGEV TIEHTTNILEDHSFTEFMGVSDGKDFDGLADCSVGEPSSRTDLTIETGRKGIPEGPPDAGQLHSKIENDQ TLHPFSEIP SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107462
ORF Size	5067 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107462.1, NP_001100932.1
RefSeq Size	6733 bp
RefSeq ORF	5070 bp
Locus ID	308173
MW	188.8 kDa

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