Sos2 (NM_001135561) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Sos1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR208299 representing NM_001135561 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCAGGCGCCGCAGCCCTACGAGTTCTTCAGCGAAGAGAACAGCCCGAAATGGCGGGGACTGTTGG TCCCGGCCCTGCGGAAGGTTCAGGAGCAAGTGCACCCCACCCTGTCGGCTAACGAAGAGTCTCTCTATTA CATCGAAGAACTCATCTTTCAACTGCTCAATAAGCTGTGCCTGGCTCAACCGAGGACTGTTCAAGATGTT GAGGAACGAGTTCAAAAGACTTTTCCTCATCCTATTGATAAATGGGCAATTGCTGATGCACAGTCTGCCA TAGAGAAACGAAAACGAAGAAATCCTCTCTTACTACCTGTGGACAAAATCCATCCTTCCTTGAAGGAAGT TTTGGGGTATAAAGTGGACTACCATGTATCCCTCTACATTGTGGCTGTATTGGAGTATATCTCAGCAGAT ATTTTGAAATTGGCTGGTAATTATGTTTTTAATATCCGGCATTATGAAATATCTCAGCAAGACATTAAAG TGTCAATGTGTGCAGATAAGGTTTTGATGGACATGTTTGACCAGGATGACGATATAGGCTTGGTTTCTCT CTGTGAAGAGGAACCTTGTTCTTCTGGTGAGCTAAACTATTATGACCTTGTTAGAACTGAAATCGCAGAA GAAAGACAGTATCTACGGGAACTGAATATGATCATTAAAGTATTCCGGGAAGCCTTTCTTTCAGACAAAA AGTTGTTCAAGTCTTCTGAAATTGAAAAGATTTTCAGTAACATTTCAGATATACATGAACTGACTGTGAA ACTTTTAGGTTTGATTGAAGACACAGTAGAAATGACAGATGAAAGCAGTCCTCACCCGCTAGCTGGTAGC TGCTTTGAAGATTTAGCAGAGGAGCAGGCTTTTGACCCCTATGAAACGTTATCACAGGACATTCTTGCTC CAGAGTTTAATGACCACTTCAGCAAGTTGATGGCCAGACCTGCAGTTGCTCTGCACTTTCAGTCCATTGC TGACGGCTTTAAGGAGGCTGTTCGATATGTCCTTCCGCGCCTCATGCTGGTGCCGGTGTATCATTGTTGG CATTACTTTGAATTATTAAAGCAGTTGAAAGCATGTAGCGAAGAACAGGAAGACCGAGAGTGTTTGAATC AGGCTATCACTGCCCTCATGAACCTCCAAGGCAGCATGGACCGGATTTACAAGCAGCACTCGCCAAGGCG CCGGCCAGGGGATCCTGTTTGCCTTTTTTACAATCGTCAATTAAGAAGCAAGCACCTGGCTATCAAAAAA ATGAATGAAATTCAGAAAAACATTGATGGGTGGGAAGGCAAAGATATTGGACAGTGTTGTAATGAGTTCA TAATGGAAGGACCATTGACCAGAATTGGTGCTAAGCATGAAAGGCATATTTTCCTCTTCGATGGCTTAAT GATCAGCTGTAAACCCAATCATTGCCAGACTCGACTTCCAGGGTATAGTAGTGCAGAATACAGGTTAAAA GAGAAGTTTGTCATGAGGAAAATTCAAATCTGTGATAAAGAAGACGCTTGTGAATACAAACATGCTTTTG AATTAGTATCCAAAGATGAAAACAGTGTAATATTTGCTGCCAAGTCAGCTGAAGAGAAAAACAACTGGAT GGCAGCCCTTATCTCCCTTCACTATCGGAGCACTCTAGATAGAATGCTGGACTCTGTGTTACTGAAAGAG GAGAACGAGCAGCCCCTGCGGCTACCGCGGCCAGACGTGTACCGCTTCGTGGTGACAGACTCAGAGGAGA ACATAGTTTTTGAAGACAACTTGCAAAGCAGAAGTGGAATCCCCATTATTAAAGGAGGCACCGTGGTGAA ACTGATCGAAAGGCTAACATATCACATGTATGCAGATCCCAATTTTGTTCGTACTTTCCTTACTACGTAT CGTTCATTTTGTAAGCCACAGGAATTGCTAAGCTTGCTGATAGAACGGTTTGAAATTCCAGATCCAGAGC CTACTGAGGCCGACAAACTGGCGCTAGAGAAAGGCGAGCAGCCAATCAGCGCAGATCTGAAAAGATTCCG CAAGGAATATGTCCAACCCGTGCAACTTAGGGTCTTGAATGTCTTTCGCCACTGGGTTGAGCATCATTTT TATGACTTTGAAAGAGACTTGGAATTGCTTGAAAGACTGGAAGCCTTCATTTCAAGTGTAAGAGGGAAAG CCATGAAGAAGTGGGTAGAATCCATTGCTAAAATCATCAAGAGGAAGAAGCAAGCTCAGGCCAATGGAGT AAGCCATAATATCACCTTTGAAAGTCCGCCTCCACCGGTTGAATGGCACATAAGTAGGGCGGGACAGTCT GAAACCTTTGACCTTATGACACTTCATCCCATAGAGATTGCACGGCAGTTAACACTCTTGGAATCTGATC TCTACAGGAGAGTCCAGCCTTCTGAACTTGTAGGGAGTGTCTGGACCAAAGAAGATAAAGAAATAAATTC TCCAAATCTATTAAAAATGATTCGCCATACAACAAACCTCACTTTGTGGTTTGAAAAATGCATTGTGGAA GCAGAAAACTTTGAAGAACGGGTGGCAGTGCTCAGCAGAATAGTAGAAATTCTGCAAGTGTTTCAAGATC TGAATAATTTCAATGGCGTGTTGGAGATAGTCAGTGCAGTCAATTCAGTGTCAGTGTACAGACTAGACCA CACATTTGAGGCATTGCAGGAAAGGAAGCGGAGAGTTTTGGATGACGCTGTGGAATTAAGTCAGGATCAC TTTAAAAAATACCTAGTAAAACTTAAATCAATCAATCCACCTTGTGTGCCTTTTTTTGGAATATATTTAA CAAATATTCTGAAGACTGAAGAAGGGAACAGCGACTTTCTAAAGAGGAAAGGGAAAGATTTGATCAATTT CAGTAAGAGGAGAAAAGTGGCTGAAATAACCGGAGAGATTCAGCAGTATCAGAACCAACCATACTGCTTA CGGACAGAACCAGAGATGAGGAGATTCTTTGAAAACCTCAATCCCATGGGACTTTTATCTGAAAAAGAGT TTACAGATTATTTGTTCAACAAATCTCATGAAATTGAACCCCGAAACTGCAAACAACCACCTCGATTTCC TAGGAAGTCAACCTTTTCCTTAAAATCTCCTGGAATAAGGCCCAATGCCAGCCGCCATGGCTCTACCTCA GGCACGTTCCGAGGTCACCCAACCCCTCTGGAAAGGGAGCCTTATAAGATAAGCTTTAGCAGGATCGCCG AGACGGAGCTAGAATCCACGGTGTCAGCACCGACATCTCCCAACACCCCATCCACCCCACCTGTGTCTGC TTCTTCAGACCACAGCGTGTTTCTAGATGTGGACCTCAATAGCTCCTGTGGCAGCAATACCATCTTTGCT CCAGTCCTCTTGCCACACTCAAAGTCTTTCTTCAGCTCATGTGGAAGTCTACACAAACTGAGTGAAGAGC CACTAGTTCCTCCTCCGCTTCCCCCTCGGAAAAAGTTTGATCACGATGCTCCGAACTCCAAGGGAGCTAT GAAATCGGATGATGACCCTCCTGCTATTCCACCAAGACAACCCCCTCCTCCAAAGGTAAAGCCAAGAGCT CCTGTCCTCACGGGTACATTTGAAGGGCCTGTGCCCAGTCCACCTCCACCTCCTCCCAGAGACCCTCTTC CTGATACTCCTCCACCAGTTCCTCTTCGGCCTCCGGAACACTTTATAAACTGTCCGTTTAATCTCCAGCC GCCTCCGCTGGGACATCCTCACAGAGACCCAGACTGGCTCAGAGACGTTAGCACATGCCCTAATTCACCA AGCACTCCTCCCCCTACGCCCTCGCCACGGATTCCACGCAGATGCCATTTGCCCAGCCCCAGTCACAACA ATCTTGCTCACCCTCCAGCTCCTCCCGTTCCACCAAGGCAGAATTCAAGCCCTCACCTACCAAAACTGCC ACCAAAGACTTACAAGCGGGAGCTTTCACACCCGCCATTGTACAGACTACCTTTGTTTGAAAATGCAGAA ACTCCTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR208299 representing NM_001135561 Red=Cloning site Green=Tags(s) MQQAPQPYEFFSEENSPKWRGLLVPALRKVQEQVHPTLSANEESLYYIEELIFQLLNKLCLAQPRTVQDV EERVQKTFPHPIDKWAIADAQSAIEKRKRRNPLLLPVDKIHPSLKEVLGYKVDYHVSLYIVAVLEYISAD ILKLAGNYVFNIRHYEISQQDIKVSMCADKVLMDMFDQDDDIGLVSLCEEEPCSSGELNYYDLVRTEIAE ERQYLRELNMIIKVFREAFLSDKKLFKSSEIEKIFSNISDIHELTVKLLGLIEDTVEMTDESSPHPLAGS CFEDLAEEQAFDPYETLSQDILAPEFNDHFSKLMARPAVALHFQSIADGFKEAVRYVLPRLMLVPVYHCW HYFELLKQLKACSEEQEDRECLNQAITALMNLQGSMDRIYKQHSPRRRPGDPVCLFYNRQLRSKHLAIKK MNEIQKNIDGWEGKDIGQCCNEFIMEGPLTRIGAKHERHIFLFDGLMISCKPNHCQTRLPGYSSAEYRLK EKFVMRKIQICDKEDACEYKHAFELVSKDENSVIFAAKSAEEKNNWMAALISLHYRSTLDRMLDSVLLKE ENEQPLRLPRPDVYRFVVTDSEENIVFEDNLQSRSGIPIIKGGTVVKLIERLTYHMYADPNFVRTFLTTY RSFCKPQELLSLLIERFEIPDPEPTEADKLALEKGEQPISADLKRFRKEYVQPVQLRVLNVFRHWVEHHF YDFERDLELLERLEAFISSVRGKAMKKWVESIAKIIKRKKQAQANGVSHNITFESPPPPVEWHISRAGQS ETFDLMTLHPIEIARQLTLLESDLYRRVQPSELVGSVWTKEDKEINSPNLLKMIRHTTNLTLWFEKCIVE AENFEERVAVLSRIVEILQVFQDLNNFNGVLEIVSAVNSVSVYRLDHTFEALQERKRRVLDDAVELSQDH FKKYLVKLKSINPPCVPFFGIYLTNILKTEEGNSDFLKRKGKDLINFSKRRKVAEITGEIQQYQNQPYCL RTEPEMRRFFENLNPMGLLSEKEFTDYLFNKSHEIEPRNCKQPPRFPRKSTFSLKSPGIRPNASRHGSTS GTFRGHPTPLEREPYKISFSRIAETELESTVSAPTSPNTPSTPPVSASSDHSVFLDVDLNSSCGSNTIFA PVLLPHSKSFFSSCGSLHKLSEEPLVPPPLPPRKKFDHDAPNSKGAMKSDDDPPAIPPRQPPPPKVKPRA PVLTGTFEGPVPSPPPPPPRDPLPDTPPPVPLRPPEHFINCPFNLQPPPLGHPHRDPDWLRDVSTCPNSP STPPPTPSPRIPRRCHLPSPSHNNLAHPPAPPVPPRQNSSPHLPKLPPKTYKRELSHPPLYRLPLFENAE TPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001135561
ORF Size	3999 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001135561.1, NP_001129033.1
RefSeq Size	5365 bp
RefSeq ORF	4002 bp
Locus ID	85384
MW	153.2 kDa
Gene Summary	mRNA expression increases after chronic clozapine treatment in the prefrontal cortex; may play a role in synaptic function [RGD, Feb 2006]

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