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Ap2b1 (NM_080583) Rat Tagged ORF Clone
CAT＃: RR209156
- TrueORF®
Ap2b1 (Myc-DDK-tagged ORF) - Rat adaptor-related protein complex 2, beta 1 subunit (Ap2b1), (10 ug)
"NM_080583" in other vectors (3)
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CNY 6,968.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR209156 representing NM_080583
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGACTCCAAGTACTTCACAACCAATAAGAAGGGAGAAATCTTTGAATTAAAAGCTGAACTCAACA ATGAAAAGAAAGAAAAGAGGAAGGAGGCTGTGAAGAAAGTGATTGCTGCTATGACCGTGGGGAAGGATGT GAGCTCTCTCTTCCCAGATGTGGTGAACTGTATGCAGACTGACAACCTGGAACTAAAGAAGCTTGTGTAC CTCTATCTGATGAACTATGCCAAGAGTCAGCCAGACATGGCCATCATGGCTGTCAACAGCTTTGTGAAGG ATTGTGAAGACCCCAATCCTTTGATTCGAGCCTTGGCAGTTAGAACCATGGGATGCATCCGGGTGGACAA GATTACAGAGTATCTCTGTGAACCCCTCCGCAAGTGCTTGAAGGATGAAGACCCCTATGTTCGGAAAACA GCAGCAGTATGCGTGGCAAAACTCCATGATATCAATGCCCAGATGGTGGAAGATCAGGGATTTCTGGATT CTCTGCGGGATCTCATAGCAGATTCAAACCCAATGGTGGTGGCTAATGCTGTAGCAGCATTGTCTGAGAT CAGTGAGTCTCACCCAAACAGCAACTTACTTGATCTGAACCCTCAGAATATCAATAAGCTGCTCACAGCC CTGAATGAGTGCACTGAGTGGGGCCAGATTTTCATCTTGGACTGCCTGTCTAATTACAACCCTAAAGATG ACCGGGAAGCTCAGAGCATCTGTGAGCGAGTGACGCCTCGGCTCTCTCATGCCAATTCTGCAGTGGTGCT TTCAGCAGTAAAAGTTCTGATGAAGTTTCTAGAGTTGTTACCCAAGGACTCTGACTACTACAATATGCTG CTAAAGAAGCTAGCGCCTCCACTTGTCACTTTGCTCTCTGGGGAGCCAGAAGTGCAGTATGTTGCCCTGA GGAACATCAACCTAATTGTCCAGAAAAGGCCTGAAATCTTGAAGCAGGAAATCAAGGTCTTCTTTGTGAA GTACAATGATCCTATCTATGTTAAACTAGAGAAGTTAGACATCATGATTCGTCTTGCATCCCAAGCCAAC ATTGCTCAGGTTCTGGCAGAACTGAAGGAATATGCCACTGAAGTTGATGTGGACTTTGTTCGCAAAGCTG TGAGGGCCATTGGACGGTGTGCCATCAAAGTGGAGCAATCAGCAGAACGCTGTGTGAGCACACTGCTTGA TCTAATCCAGACCAAAGTAAATTATGTGGTCCAAGAGGCAATTGTTGTCATCAGGGACATCTTCCGAAAA TACCCCAACAAGTATGAGAGCATTATCGCCACGCTGTGTGAGAACTTGGACTCCCTGGATGAACCCGATG CCCGAGCGGCTATGATTTGGATTGTAGGAGAGTATGCTGAAAGAATCGATAATGCCGATGAGTTACTAGA GAGCTTCCTGGAAGGTTTTCATGATGAAAGCACCCAGGTGCAGCTCACGTTGCTTACCGCCATAGTGAAA CTGTTTCTCAAGAAGCCATCAGAAACACAGGAGCTGGTCCAACAGGTCTTGAGCTTGGCCACACAGGATT CTGATAATCCTGACCTTCGAGATCGGGGTTATATTTATTGGCGCCTTCTTTCAACTGACCCTGTGACAGC CAAAGAAGTAGTGTTGTCTGAGAAGCCATTGATCTCTGAGGAAACAGACCTCATTGAACCTACCCTCCTG GATGAGCTCATCTGCCACATTGGTTCTTTGGCCTCCGTGTACCATAAACCTCCGAATGCTTTTGTGGAAG GGAGCCATGGCATTCATCGCAAACACTTGCCAATTCACCATGGGAGCACTGATGCAGGTGATAGCCCTGT TGGCACCACCACTGCAACCAACCTGGAACAGCCTCAGGTCATCCCCTCTCAAGGTGACCTTCTGGGGGAT CTTTTAAATCTTGACCTGGGTCCCCCAGTGAATGTCCCGCAAGTGTCCTCCATGCAGATGGGAGCAGTGG ATCTTTTAGGAGGAGGACTGGATAGCCTGCTTGGCAGTGACCTTGGCGGGGGCATTGGAGGAAGTCCGGC AGTAGGACAGTCCTTCATCCCGTCATCAGTGCCTGCAACCTTCGCTCCTTCACCTACTCCTGCTGTGGTC AGCAGTGGTCTGAATGACCTGTTTGAGCTTTCCACTGGGATAGGCATGGCACCTGGCGGATATGTGGCTC CTAAGGCAGTCTGGCTACCTGCTGTAAAGGCTAAAGGCTTGGAGATTTCGGGGACGTTTACTCACCGCCA AGGGCACATCTATATGGAAATGAACTTCACCAACAAAGCTCTGCAGCACATGACGGATTTTGCCATCCAG TTTAACAAGAATAGCTTCGGTGTCATCCCGAGCACTCCCTTGGCCATACATACTCCGCTGATGCCAAACC AGAGCATTGATGTGTCTCTGCCTCTCAACACCTTGGGCCCAGTCATGAAGATGGAGCCTCTGAATAACTT GCAGGTGGCTGTTAAAAACAATATTGATGTCTTCTACTTCAGCTGCCTCATCCCACTCAATGTGCTTTTT GTAGAAGATGGCAAAATGGAACGCCAGGTCTTCCTTGCGACGTGGAAGGATATTCCCAATGAAAATGAGC TCCAATTTCAGATTAAGGAGTGTCATTTAAACGCTGACACAGTTTCCAGCAAGTTGCAAAACAACAATGT TTACACTATCGCCAAGAGGAATGTGGAGGGGCAGGACATGCTGTACCAGTCCCTGAAGCTCACTAATGGC ATTTGGATTTTGGCAGAGCTGCGGATCCAGCCAGGAAACCCCAATTATACGCTGTCGCTGAAGTGTAGAG CCCCTGAAGTCTCTCAGTACATCTATCAGGTCTACGACAGCATTTTGAAAAACCCCCTGAAGTCTCTCAG TACATCTATCAGGTCTACGACAGCATTTTGAAAAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR209156 representing NM_080583
Red=Cloning site Green=Tags(s) MTDSKYFTTNKKGEIFELKAELNNEKKEKRKEAVKKVIAAMTVGKDVSSLFPDVVNCMQTDNLELKKLVY LYLMNYAKSQPDMAIMAVNSFVKDCEDPNPLIRALAVRTMGCIRVDKITEYLCEPLRKCLKDEDPYVRKT AAVCVAKLHDINAQMVEDQGFLDSLRDLIADSNPMVVANAVAALSEISESHPNSNLLDLNPQNINKLLTA LNECTEWGQIFILDCLSNYNPKDDREAQSICERVTPRLSHANSAVVLSAVKVLMKFLELLPKDSDYYNML LKKLAPPLVTLLSGEPEVQYVALRNINLIVQKRPEILKQEIKVFFVKYNDPIYVKLEKLDIMIRLASQAN IAQVLAELKEYATEVDVDFVRKAVRAIGRCAIKVEQSAERCVSTLLDLIQTKVNYVVQEAIVVIRDIFRK YPNKYESIIATLCENLDSLDEPDARAAMIWIVGEYAERIDNADELLESFLEGFHDESTQVQLTLLTAIVK LFLKKPSETQELVQQVLSLATQDSDNPDLRDRGYIYWRLLSTDPVTAKEVVLSEKPLISEETDLIEPTLL DELICHIGSLASVYHKPPNAFVEGSHGIHRKHLPIHHGSTDAGDSPVGTTTATNLEQPQVIPSQGDLLGD LLNLDLGPPVNVPQVSSMQMGAVDLLGGGLDSLLGSDLGGGIGGSPAVGQSFIPSSVPATFAPSPTPAVV SSGLNDLFELSTGIGMAPGGYVAPKAVWLPAVKAKGLEISGTFTHRQGHIYMEMNFTNKALQHMTDFAIQ FNKNSFGVIPSTPLAIHTPLMPNQSIDVSLPLNTLGPVMKMEPLNNLQVAVKNNIDVFYFSCLIPLNVLF VEDGKMERQVFLATWKDIPNENELQFQIKECHLNADTVSSKLQNNNVYTIAKRNVEGQDMLYQSLKLTNG IWILAELRIQPGNPNYTLSLKCRAPEVSQYIYQVYDSILKNPLKSLSTSIRSTTAF*K TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_080583 |
| ORF Size | 2853 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 5413 bp |
| RefSeq ORF | 2856 bp |
| Locus ID | 140670 |
| UniProt ID | P62944 |
| MW | 105.7 kDa |
| Gene Summary | subunit of the clathrin-associated protein complex 2 [RGD, Feb 2006] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
µ2-Dependent Endocytosis of N-cadherin Is Regulated by ß-Catenin to Facilitate Neurite Outgrowth
,Chen, YT;Tai, CY;,
Traffic
,PubMed ID 28224728
[AP2B1]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN209156 | Ap2b1 (untagged ORF) - Rat adaptor-related protein complex 2, beta 1 subunit (Ap2b1), (10 ug) |
CNY 12,260.00 |
|
| RR209156L3 | Lenti ORF clone of Ap2b1 (Myc-DDK-tagged ORF) - Rat adaptor-related protein complex 2, beta 1 subunit (Ap2b1), (10 ug) |
CNY 13,870.00 |
|
| RR209156L4 | Lenti ORF clone of Ap2b1 (mGFP-tagged ORF) - Rat adaptor-related protein complex 2, beta 1 subunit (Ap2b1), (10 ug) |
CNY 15,300.00 |
