Plcb4 (NM_024353) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Beta4aa; RATBETA4AA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR210250 representing NM_024353 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAAACCTTACGAATTTAACTGGCAGAAGGAGGTACCCTCTTTCTTGCAAGAAGGAGCAGTTTTTG ACAGATATGAAGAGGAATCTTTCGTGTTTGAGCCCAACTGCCTCTTCAAAGTGGATGAATTCGGCTTCTT CCTGACCTGGAAGAGTGAAGGCAAGGAAGGACAAGTGCTAGAATGTTCCCTCATCAACAGCATTCGGCTG GCAGCCATACCAAAGGATCCCAAAATCCTGGCTGCTCTCGAGTCTGTTGGAAAATCTGAAAATGATCTGG AAGGGCGGATATTGTGTGTCTGCAGCGGCACGGATCTGGTGAATATTGGCTTCACTTACATGGTGGCTGA AAACCCAGAAATAACTAAGCAATGGGTAGAAGGCCTGAGATCAATCATTCACAACTTCAGGGCAAACAAC GTCAGTCCGATGACATGCCTCAAGAAACACTGGATGAAACTGGCCTTTCTGACCAACACAAGTGGCAAAA TCCCAGTGAGGAGTATTACTAGAACCTTCGCATCAGGGAAAACAGAAAAGGTGATCTTTCAAGCACTCAA GGAATTAGGTCTCCCCAGTGGAAAGAATGATGAAATTGAACCTGCTGCATTTACCTATGAAAAGTTCTAT GAACTGACACAGAAGATTTGTCCTCGGACAGATATAGAAGATCTTTTTAAAAAAATCAATGGAGACAAAA CTGATTATTTAACGGTAGACCAATTAGTGAGCTTTCTAAACGAACATCAGCGAGATCCTCGGCTGAATGA AATTTTATTCCCGTTTTATGATGCTAAAAGAGCAATGCAGATCATTGAGATGTATGAACCTGATGAAGAG TTGAAGAAAAAAGGCCTCATATCCAGTGATGGATTCTGCAGATATCTCATGTCAGATGAAAATGCCCCCG TCTTCCTAGATCGCTTAGAACTTTACCAGGAGATGGACCACCCCCTGGCCCATTACTTCATCAGTTCCTC TCACAACACCTATCTCACCGGCCGGCAGTTTGGCGGAAAGTCTTCAGTGGAAATGTACCGACAAGTCCTC CTGGCTGGTTGCAGGTGTGTGGAACTTGACTGTTGGGATGGAAAAGGTGAAGATCAGGAACCAATAATAA CTCACGGAAAAGCAATGTGTACAGACATCCTTTTTAAGGATGTAATCCAGGCCATCAAGGAAACAGCGTT TGTCACATCAGAATATCCTGTAATTCTCTCCTTTGAAAACCACTGCAGCAAATATCAACAGTACCAGATG TCCAAGTATTGTGAAGATCTATTTGGGGATCTTCTGTTGAAACAAGCACTTGAGTCACATCCACTTGAAC CAGGGAGGCTCTTGCCATCTCCTAATGACCTCAAAAGAAAAATACTCATCAAAAATAAAAGGCTGAAGCC TGAAGTTGAAAAAAAACAGCTGGAGGCTTTGAAAAGCATGATGGAGGCTGGAGAATCAGCTGCCCCGGCC AGCATCTTGGAAGATGACAATGAGGAGGAAATAGAAAGCGCAGCTGATCAAGAGGAAGAAGCCCACCCTG AATACAAGTTTGGAAATGAACTTTCTGCCGATGACTTCAGTCACAAGGAAGCGGTTGCAAACAGCGTCAA GAAGGGCCTGGTCACCGTAGAAGATGAGCAGGCATGGATGGCATCTTATAAATACGTAGGTGCTACCACG AATATCCATCCGTACTTGTCCACGATGATCAACTATGCACAGCCTGTGAAGTTTCAAGGTTTCCATGTGG CTGAAGAGCGCAACATTCATTATAACATGTCTTCTTTTAACGAGTCGGTTGGCCTTGGCTACTTGAAGAC GCACGCGATTGAGTTTGTAAATTACAATAAGCGACAAATGAGCCGCATTTACCCCAAGGGAGGGCGAGTT GATTCCAGTAATTACATGCCTCAGATTTTCTGGAACGCTGGTTGCCAGATGGTTTCACTGAACTATCAAA CCCCAGATTTAGCGATGCAATTGAATCAAGGAAAATTTGAGTATAACGGATCCTGCGGATACCTCCTCAA GCCAGACTTCATGAGACGGCCTGATCGGACATTTGATCCCTTCTCTGAAACCCCTGTGGACGGGGTTATT GCAGCCACATGCTCAGTGCAGGTTATATCAGGGCAGTTCCTGTCAGATAAGAAGATCGGCACATACGTGG AAGTCGATATGTACGGGCTGCCCACCGACACCATACGGAAGGAGTTCCGAACCCGCATGGTTATGAACAA TGGACTCAACCCAGTTTACAATGAAGAATCGTTCGTGTTTCGGAAGGTGATCCTGCCTGACCTGGCTGTC CTGAGAATCGCTGTGTACGACGACAACAACAAGCTCATTGGCCAGAGGATCCTTCCTCTGGATGGCCTCC AGGCAGGTTACCGACACATCTCCCTGAGAAATGAGGGAAACAAACCGTTATCACTGCCAACAATTTTCTG CAATATCGTTCTTAAAACATATGTGCCTGATGGATTGGGAGATATTGTGGATGCTCTATCCGATCCAAAG AAATTCCTTTCAATCACAGAGAAGAGAGCAGACCAATTGAGAGCAATGGGCATTGAAACTAGTGACATAG CAGATGTGCCCAGTGACACTTCCAAAAATGACAAGAAAGGCAAGGCCAACCCAGCCAAAGCGAATGTAAC CCCTCAGAGCAGCTCTGAGCTCAGACCAACCACCACAGCTGCCCTGGGATCTGGCCAGGAAGCCAAGAAA GGTATTGAACTTATCCCTCAAGTGAGGATAGAAGATTTAAAGCAAATGAAGGCTTACTTGAAGCATTTAA AGAAACAACAAAAGGAGTTAAACTCTTTAAAGAAGAAACACGCAAAGGAGCACAGTACCATGCAGAAGTT ACACTGCACACAAGTGGACAAAATCGTGGCCCAGTATGACAAGGAGAAGTCGACTCATGAGAAAATCCTA GAGAAGGCCATGAAGAAGAAGGGGGGAAGTAATTGCCTTGAAATAAAAAAAGAAACAGAAATTAAGATTC AGACCCTGACATCGGATCACAAATCTAAGGTCAAGGAGATTGTGGCGCAGCACACAAAGGAGTGGTCAGA AATGATCAACACGCACAGCGCCGAGGAGCAGGAAATCCGGGACCTGCACCTGAGCCAGCAGTGTGAGCTG CTGAGGAAGCTGCTCATCAACGCTCATGAGCAGCAGACCCAGCAGCTGAAGCTCTCCCATGACAGGGAAA GCAAAGAGATGCGAGCCCATCAGGCTAAGATTTCTATGGAAAATAGCAAAGCCATCAGTCAAGATAAATC TATCAAGAATAAGGCAGAACGGGAGAGGCGAGTCAGGGAGTTGAACAGCAGCAACACTAAGAAGTTTCTA GAAGAAAGAAAGAGACTGGCAATGAAGCAGTCGAAAGAAATGGATCAGCTGAAAAAGGTCCAGCTGGAGC ACCTAGAATTCCTAGAGAAACAGAATGAGCAGGCGAAGGAGATGCAGCAGATGGTGAAATTGGAAGCCGA GATGGACCGCAGACCAGCAACAGTAGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR210250 representing NM_024353 Red=Cloning site Green=Tags(s) MAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWKSEGKEGQVLECSLINSIRL AAIPKDPKILAALESVGKSENDLEGRILCVCSGTDLVNIGFTYMVAENPEITKQWVEGLRSIIHNFRANN VSPMTCLKKHWMKLAFLTNTSGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAAFTYEKFY ELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEE LKKKGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVL LAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYQM SKYCEDLFGDLLLKQALESHPLEPGRLLPSPNDLKRKILIKNKRLKPEVEKKQLEALKSMMEAGESAAPA SILEDDNEEEIESAADQEEEAHPEYKFGNELSADDFSHKEAVANSVKKGLVTVEDEQAWMASYKYVGATT NIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRV DSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVI AATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAV LRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGLGDIVDALSDPK KFLSITEKRADQLRAMGIETSDIADVPSDTSKNDKKGKANPAKANVTPQSSSELRPTTTAALGSGQEAKK GIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKSTHEKIL EKAMKKKGGSNCLEIKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSEMINTHSAEEQEIRDLHLSQQCEL LRKLLINAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFL EERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQAKEMQQMVKLEAEMDRRPATVV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_024353
ORF Size	3528 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_024353.1, NP_077329.1
RefSeq Size	5297 bp
RefSeq ORF	3531 bp
Locus ID	25031
UniProt ID	Q9QW07
MW	134.5 kDa
Gene Summary	phospholipase C isozyme; may play a role in phototransduction [RGD, Feb 2006]

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