United States
Germany
Japan
United Kingdom
China
Mdh1 (NM_033235) Rat Tagged ORF Clone
CAT＃: RR210984
- TrueORF®
Mdh1 (Myc-DDK-tagged ORF) - Rat malate dehydrogenase 1, NAD (soluble) (Mdh1), (10 ug)
"NM_033235" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Mdhl; MDL1; Mor2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR210984 representing NM_033235
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGAGCCAATCAGAGTCCTCGTGACAGGAGCAGCCGGTCAGATTGCATATTCGCTGCTGTACAGCA TTGGAAATGGATCTGTCTTTGGGAAAGACCAGCCCATCATTCTTGTGCTGTTGGACATCACCCCCATGAT GGGTGTTCTGGACGGTGTCCTGATGGAGCTGCAAGACTGTGCCCTTCCCCTTCTGCAGGATGTCATTGCA ACAGACAAAGAAGAGGTTGCCTTCAAAGACCTGGACGTGGCTGTCCTTGTGGGCTCCATGCCAAGAAGGG AGGGCATGGAGAGGAAGGACCTACTGAAAGCCAACGTGAAGATCTTCAAATCCCAGGGCGCAGCCTTGGA GAAGTACGCCAAGAAATCAGTTAAGGTCATTGTTGTGGGGAACCCAGCCAATACAAACTGCCTGACGGCC TCCAAGTCAGCACCATCGATCCCCAAGGAGAACTTCAGTTGCCTGACTCGATTGGACCACAACCGAGCAA AATCTCAAATTGCTCTTAAACTCGGTGTAACCGCTGATGATGTAAAAAATGTCATTATCTGGGGAAATCA TTCATCAACCCAGTATCCAGATGTCAATCATGCCAAGGTGAAATTGCAAGGAAAAGAAGTTGGTGTGTAT GAAGCCCTCAAAGACGACAGCTGGCTCAAGGGAGAGTTCATCACGACTGTGCAGCAGCGTGGTGCTGCTG TCATCAAGGCTCGGAAGCTGTCCAGTGCCATGTCTGCTGCGAAGGCCATCTCGGACCACATCAGAGACAT CTGGTTTGGAACCCCGGAGGGCGAGTTCGTGTCGATGGGCGTAATCTCTGATGGCAACTCCTATGGTGTC CCTGATGACCTGCTCTACTCGTTCCCTGTCGTGATCAAGAATAAGACCTGGAAGTTTGTTGAAGGCCTCC CCATTAACGACTTCTCCCGTGAGAAGATGGACCTGACAGCAAAGGAGCTGACCGAGGAAAAGGAAACGGC TTTTGAGTTTCTCTCCTCCGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210984 representing NM_033235
Red=Cloning site Green=Tags(s) MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLQDVIA TDKEEVAFKDLDVAVLVGSMPRREGMERKDLLKANVKIFKSQGAALEKYAKKSVKVIVVGNPANTNCLTA SKSAPSIPKENFSCLTRLDHNRAKSQIALKLGVTADDVKNVIIWGNHSSTQYPDVNHAKVKLQGKEVGVY EALKDDSWLKGEFITTVQQRGAAVIKARKLSSAMSAAKAISDHIRDIWFGTPEGEFVSMGVISDGNSYGV PDDLLYSFPVVIKNKTWKFVEGLPINDFSREKMDLTAKELTEEKETAFEFLSSA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033235 |
| ORF Size | 1002 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_033235.2, NP_150238.1 |
| RefSeq Size | 1266 bp |
| RefSeq ORF | 1005 bp |
| Locus ID | 24551 |
| UniProt ID | O88989 |
| MW | 36.5 kDa |
| Gene Summary | This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. [provided by RefSeq, Feb 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN210984 | Mdh1 (untagged ORF) - Rat malate dehydrogenase 1, NAD (soluble) (Mdh1), (10 ug) |
CNY 3,990.00 |
|
| RR210984L3 | Lenti ORF clone of Mdh1 (Myc-DDK-tagged ORF) - Rat malate dehydrogenase 1, NAD (soluble) (Mdh1), (10 ug) |
CNY 6,080.00 |
|
| RR210984L4 | Lenti ORF clone of Mdh1 (mGFP-tagged ORF) - Rat malate dehydrogenase 1, NAD (soluble) (Mdh1), (10 ug) |
CNY 6,650.00 |
