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Hif1a (NM_024359) Rat Tagged ORF Clone
CAT＃: RR210999
- TrueORF®
Hif1a (Myc-DDK-tagged ORF) - Rat hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (Hif1a), (10 ug)
"NM_024359" in other vectors (3)
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CNY 10,550.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MOP1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR210999 representing NM_024359
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGCGCCGGCGGCGAGAACGAGAAGAAAAATAGGATGAGTTCCGAACGTCGAAAAGAAAAGTCTA GGGATGCAGCACGATCTCGGCGAAGCAAAGAGTCTGAAGTTTTTTATGAGCTTGCTCATCAGTTGCCACT TCCCCACAACGTGAGCTCCCATCTTGATAAAGCTTCTGTTATGAGGCTCACCATCAGTTACTTACGTGTG AGGAAACTTCTAGGTGCTGGTGATCTTGACATTGAAGATGAAATGAAAGCACAGATGAACTGCTTTTATC TGAAAGCCCTGGATGGCTTTGTTATGGTGCTAACAGATGATGGTGACATGATTTACATTTCTGATAACGT GAACAAATACATGGGGTTGACTCAGTTTGAACTAACTGGACACAGTGTGTTTGATTTTACCCATCCATGT GACCATGAGGAAATGAGAGAAATGCTTACACACAGAAATGGCCCAGTGAGAAAGGGGAAAGAACAAAACA CGCAGCGAAGCTTTTTTCTCAGAATGAAATGTACCCTAACAAGCCGGGGGAGGACGATGAACATCAAGTC AGCAACGTGGAAGGTGCTGCACTGCACAGGCCACATTCATGTGTATGATACCAGCAGTAACCAGCCGCAG TGTGGCTACAAGAAACCGCCTATGACGTGCTTGGTGCTGATTTGTGAACCCATTCCTCATCCATCAAACA TTGAAATTCCTTTAGACAGCAAGACATTTCTCAGTCGACACAGCCTCGATATGAAATTTTCTTACTGTGA TGAAAGGATTACTGAGTTGATGGGTTATGAGCCAGAAGAACTTTTGGGCCGTTCAATTTATGAATATTAT CATGCTTTGGACTCTGATCATCTGACCAAAACTCATCATGACATGTTTACTAAAGGACAAGTCACCACAG GACAGTACAGGATGCTTGCAAAAAGAGGTGGATATGTCTGGGTTGAGACTCAAGCAACTGTTATATATAA TACGAAGAACTCTCAGCCACAGTGCATTGTGTGTGTGAATTATGTTGTAAGTGGTATTATTCAGCACGAC TTGATTTTCTCCCTTCAACAAACAGAATCTGTCCTCAAACCAGTTGAATCTTCAGATATGAAAATGACCC AGCTGTTCACTAAAGTGGAATCTGAGGACACGAGCTGCCTCTTCGACAAGCTTAAGAAAGAGCCCGATGC CCTGACTCTGCTAGCTCCAGCGGCTGGGGACACGATCATATCACTGGACTTCGGCAGCGATGACACGGAA ACTGAAGACCAACAACTTGAAGATGTCCCGTTGTACAATGATGTAATGTTCCCCTCTTCTAATGAGAAAT TAAATATAAATCTGGCAATGTCTCCATTACCTGCCTCTGAAACTCCAAAGCCACTTCGAAGTAGTGCTGA TCCTGCACTGAATCAAGAGGTTGCATTGAAGTTAGAGTCAAGCCCAGAGTCACTGGGACTTTCTTTTACC ATGCCCCAGATTCAAGATCAGCCAGCAAGTCCTTCTGATGGAAGCACTAGACAAAGCTCACCTGAGCCTA ACAGTCCCAGTGAGTACTGCTTTGATGTGGACAGCGATATGGTCAATGTATTCAAGTTGGAACTGGTGGA AAAACTGTTTGCTGAAGACACAGAAGCGAAGAATCCATTTTCAGCTCAGGACACTGATTTAGACTTGGAA ATGCTGGCTCCCTATATCCCAATGGATGATGATTTCCAGTTACGTTCCTTTGATCAGTTGTCACCATTAG AGAGCAATTCTCCAAGCCCTCCGAGTGTGAGCACAGTTACAGGATTCCAGCAGACCCAGTTACAGAAACC TACCATCACTGTCACTGCCACCGCAACTGCCACCACTGATGAATCAAAAGCAGTGACGAAGGACAATATA GAAGACATTAAAATACTGATTGCATCTCCACCTTCTACCCAAGTACCTCAAGAAATGACCACTGCTAAGG CATCAGCATACAGTGGTACTCACAGTCGGACAGCCTCACCAGACAGAGCAGGAAAGAGAGTCATAGAAAA AACAGACAAAGCTCATCCAAGGAGCCTTAACCTATCTGTCACTTTGAATCAAAGAAATACTGTTCCTGAA GAAGAATTAAACCCAAAGACAATAGCTTTGCAGAATGCTCAGAGGAAGCGAAAAATGGAACATGATGGCT CCCTTTTTCAAGCAGCAGGAATTGGAACGTTACTGCAGCAACCAGGTGACCGTGCCCCTACTATGTCGCT TTCTTGGAAACGAGTGAAAGGATACATATCTAGTGAACAGGATGGAATGGAGCAGAAGACAATTTTTTTA ATACCCTCTGATTTAGCATGTAGACTGCTGGGGCAGTCAATGGATGAGAGTGGATTACCACAGCTGACCA GTTACGATTGTGAAGTTAATGCTCCCATACAAGGCAGCAGAAACCTACTGCAGGGTGAAGAATTACTCAG AGCTTTGGATCAAGTTAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210999 representing NM_024359
Red=Cloning site Green=Tags(s) MEGAGGENEKKNRMSSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRV RKLLGAGDLDIEDEMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPC DHEEMREMLTHRNGPVRKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTSSNQPQ CGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYY HALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHD LIFSLQQTESVLKPVESSDMKMTQLFTKVESEDTSCLFDKLKKEPDALTLLAPAAGDTIISLDFGSDDTE TEDQQLEDVPLYNDVMFPSSNEKLNINLAMSPLPASETPKPLRSSADPALNQEVALKLESSPESLGLSFT MPQIQDQPASPSDGSTRQSSPEPNSPSEYCFDVDSDMVNVFKLELVEKLFAEDTEAKNPFSAQDTDLDLE MLAPYIPMDDDFQLRSFDQLSPLESNSPSPPSVSTVTGFQQTQLQKPTITVTATATATTDESKAVTKDNI EDIKILIASPPSTQVPQEMTTAKASAYSGTHSRTASPDRAGKRVIEKTDKAHPRSLNLSVTLNQRNTVPE EELNPKTIALQNAQRKRKMEHDGSLFQAAGIGTLLQQPGDRAPTMSLSWKRVKGYISSEQDGMEQKTIFL IPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024359 |
| ORF Size | 2469 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024359.1, NP_077335.1 |
| RefSeq Size | 3718 bp |
| RefSeq ORF | 2472 bp |
| Locus ID | 29560 |
| UniProt ID | O35800 |
| MW | 92.1 kDa |
| Gene Summary | regulates transcription in response to low oxygen; may play a role in vascular biology [RGD, Feb 2006] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Effect of hypoxia on tissue factor pathway inhibitor expression in breast cancer.
,null,
Journal of thrombosis and haemostasis : JTH
,PubMed ID 26598923
[Hif1a]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN210999 | Hif1a (untagged ORF) - Rat hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (Hif1a), (10 ug) |
CNY 9,656.00 |
|
| RR210999L3 | Lenti ORF clone of Hif1a (Myc-DDK-tagged ORF) - Rat hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (Hif1a), (10 ug) |
CNY 12,350.00 |
|
| RR210999L4 | Lenti ORF clone of Hif1a (mGFP-tagged ORF) - Rat hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (Hif1a), (10 ug) |
CNY 13,490.00 |
