Pole (NM_001107152) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	POLE1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR212034 representing NM_001107152 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCTGAGGAACAGTGGACGGAGGCATCCCGAGCCGGGCGCGGACAGCGAAGGCAGCCGGGATGATG GTCCCTCCTCTTCAGTCTCAGCACTCAAGCGTCTGGAACGGAGCCAGTGGACGGATAAGATGGACTTACG GTTTGGTTTTGAAAGGTTGAAAGAGCCTGGAGAAAGGACTGGCTGGCTTATCAACATGCACCCTACAGAG ATTTTAGATGAAGACAAACGCTTAGTCAGTGCAGTGGATTACTACTTCATTCAAGATGATGGAAGCAGAT TTAAGGTGGCCTTGCCCTACAAGCCATATTTCTACATTGCAGCTAGAAAGGGTTGTGATCGAGAAGTTTC ATCTTTTCTATCCAAGAAGTTTCAGGGTAAAATTGCAAAGTTAGAGAATGTGCCCAAAGAAGACCTGGAC TTGCCAAACCACTTGGTGGGCTTGAAGCGGAGTTACATCAAGCTGTCCTTCCACACTGTGGAGGATCTTG TCAAAGTGAGAAAGGAGATCTCTCCTGCTGTGAAGAAGAACCGAGAGCAGGACCATGCTAGTGACGAGTA CACAACAATGCTCTCCAGTATTCTGCAAGGAGGCAGTCTAATTACTGATGAGGAGGAAACCTCTAAGAAG ATAGCTGACCAATTGGACAACATAGTGGACATGCGGGAGTATGATGTTCCCTACCACATTCGCCTCTCCA TTGACCTCAGAATCCATGTGGCCCACTGGTACAATGTTAGATTTCGAGGAAATGCTTTTCCTGTGGAAAT TGCCCGACGAGATGATCTCGTCGAACGACCTGATCCTGTGGTTTTGGCATTTGACATTGAGACAACCAAA CTACCTCTCAAATTCCCTGATGCTGAGACAGATCAGATTATGATGATCTCCTATATGATTGATGGCCAGG GCTACCTCATCACTAACAGGGAGATTGTTTCAGAAGACATTGAAGATTTTGAGTTCACCCCCAAGCCAGA ATATGAAGGGCCTTTCTGTGTCTTCAATGAACCTGATGAGGTCCATCTGATCCAGAGATGGTTTGAGCAT ATCCAGGAGACCAAACCTACCATTATGGTCACCTACAATGGGGATTTTTTTGACTGGCCGTTTGTGGAGG CCAGGGCAGCAATTCATGGCCTAAGCATGTACCAGGAGATAGGCTTCCAGAAGGATAGCCAGGGGGAATA TAAGGCACCACAGTGCATCCACATGGACTGCCTCAGGTGGGTGAAGAGGGACAGTTACCTTCCTGTGGGC AGTCATAATCTCAAGGCAGCTGCCAAAGCCAAACTTGGCTATGACCCTGTGGAGCTGGACCCTGAGGACA TGTGCCGTATGGCCACTGAACAGCCCCAGACTCTGGCCACTTACTCAGTGTCAGATGCTGTTGCTACTTA TTACCTGTACATGAAATATGTGCACCCCTTCATATTCGCCCTCTGCACCATTATTCCCATGGAACCTGAT GAGGTGCTGCGGAAGGGCTCTGGAACATTGTGCGAAGCCTTGCTGATGGTGCAAGCCTTCCATGCCAACA TTATCTTTCCCAATAAGCAAGAGCAGGAGTTCAACAAGCTGACAGATGATGGCCACGTGCTAGATGCTGA GACCTACGTTGGGGGCCATGTGGAGGCCCTAGAGTCTGGTGTCTTCCGAAGTGATATCCCCTGCCGGTTT AGGATGAATCCTGCAGCCTTTGATTTCCTGCTGCAGCGAGTTGAGAAGACTATGCGCCATGCCATTGAAG AAGAAGAGAAGGTGCCTGTGGAACAAGCCACCAACTTCCAAGAGGTGTGTGAGCAGATTAAGACCAAACT CACCTCCCTAAAAGATGTTCCTAACAGAATTGAATGTCCTCTAATCTACCATCTAGATGTGGGGGCCATG TATCCTAACATAATCCTCACCAACCGCCTGCAGCCTTCTGCCATAGTGGATGAGGCCACCTGTGCTGCCT GTGACTTCAATAAGCCTGGAGCAACTTGTCAGAGGAAAATGGCCTGGCAATGGAGGGGAGAATTCATGCC AGCCAGTCGTAGTGAGTACCATCGAATCCAGCATCAGCTAGAGTCGGAGAAGTTCCCCCCTTTGTTCCCA GAGGGGCCAGCAAGGGCCTTTCATGAGCTGTCCCGTGAAGAACAGGCTAAATACGAGAAACGGAGGCTGG CAGATTATTGCCGGAAAGCCTATAAGAAGATCCATATGACCAAGGTAGAAGAGCGCCTGACTACCATCTG CCAGCGGGAAAACTCATTTTATGTGGACACAGTGCGGGCCTTCAGAGACAGGCGCTATGAATTCAAAGGA CTGCACAAGGTGTGGAAGAAGAAGCTCTCGGCGGCTGTAGAGGTGGGCGATGCATCAGAGGTGAAGCGCT GCAAGAACATGGAGATCCTTTATGATTCGCTGCAGCTGGCTCACAAATGCATCCTGAACTCATTCTACGG CTATGTTATGCGCAAAGGAGCTCGCTGGTACTCCATGGAGATGGCTGGTATCGTCTGCTTTACAGGAGCC AACATCATCACCCAGGCAAGAGAGCTGATTGAGCAGATCGGGAGGCCTTTAGAACTGGACACGGACGGAA TATGGTGCGTTCTACCCAATAGCTTTCCTGAAAATTTTGTCATCAAGACAACCAATGTGAAAAAACCCAA ACTGACCATCTCCTATCCTGGTGCCATGTTGAACATCATGGTCAAGGAAGGCTTTACCAACCACCAGTAC CAGGAACTAACAGAGCCTTCGTCTCTCACCTATGTCACCCGCTCTGAGAACAGCATCTTTTTTGAAGTTG ATGGACCATACCTTGCTATGATCCTTCCAGCCTCCAAGGAAGAAGGCAAGAAGCTGAAGAAAAGATATGC TGTATTCAATGAAGATGGCTCCTTGGCTGAACTGAAAGGTTTTGAGGTGAAACGCCGAGGGGAGTTGCAG TTGATTAAAATCTTCCAGTCCTCAGTGTTTGAGGCCTTCCTCAAGGGCAGCACACTAGAGGAGGTGTATG CCTCTGTGGCCAAAGTGGCTGACTACTGGCTAGATGTGCTCTATAGCAAGGCTGCTAATATGCCCGATTC TGAATTATTCGAACTGATTTCTGAGAACCGCTCCATGTCACGGAAGCTAGAAGATTATGGGGAGCAGAAG TCTACGTCCATCAGCACAGCAAAGCGCCTGGCTGAGTTCCTGGGAGACCAGATGGTCAAAGATGCTGGAC TGAGCTGCCGCTACATTATCTCCCGAAAGCCAGAGGGATCTCCTGTCACTGAGAGGGCCATTCCACTTGC CATTTTCCAAGCAGAGCCTACAGTGAGGAAACATTTTCTCCGGAAATGGTTAAAGAGTTCATCACTTCAA GACTTTGATATTCGGACAATTCTGGACTGGGACTACTACATAGAGCGGCTGGGGAGTGCCATCCAGAAAA TCATCACCATCCCTGCAGCTCTACAGCAGGTGAAGAACCCAGTTCCACGTGTCAAACATCCAGACTGGCT GCACAAAAAGCTACTAGAGAAGAATGATATCTACAAACAGAAGAAGATCAGTGAGCTCTTTGTGCTTGAA GGAAAGAGACAGATTGTGATGGCCCAGGCTTCAGAAAACAGTCTGAGTCTCTGCACTCCAGACATGGAGG ACTTTGGACTCACAAAGCCATATCACTCTACAGTTCCAGTTGCTACTAAGAGGAAGCGAGTCTGGGAGAG CCAAAAGGAGTCTCAGGATATTACACTAACTGTGCCCTGGCAAGAGGTCTTAGGGCAGCCTCCCTCCCTT GGAACCACACAGGAAGAGTGGTTGGTCTGGCTCCAGTTCCACAAGAAAAAGTGGCAGCTGCAGGCTCAAC AGCGCCTAGCCCGCAGGAAGAAACAGCGCTTAGAGTCAGCAGAAGATATGCCAAGGCTTGGGGCTATCCG AGAGGGGCCTGCCACAGGACTGGGGAGCTTTCTGCGACGGACCGCCCGCAGCATCATGGACCTCCCCTGG CAGATCATACAGATCAGTGAGACCAGACAGGCTGGTCTGTTCCGGCTGTGGGCTATCATTGGCAGCGACT TGCACTGCATCAAGCTGAGTATCCCTCGAGTATTCTATGTAAACCAGCGGGTAGCCAAAGCAGAGGATGG GCCTTCGTATCGAAAGGTGAATCGGGCTCTTCCTCGTTCCAACATCGTCTACAATCTCTATGAGTACTCA GTACCAGAGGACATGTACCAAGAACACATCAACGAGATCAACACTGAGTTGTCAGCACCAGACATTGAGG GCGTGTATGAGACTCAGGTCCCATTGTTATTCCGGGCCCTGGTGCAGCTGGGCTGTGTGTGTGTGGTCAA CAAACAGCTGGCAAGGCAACTTTCAGGCTGGGAAGCTGAAACCTTTGCCCTCGAGCACCTTGAAATGCGT TCTCTGGCCCAGTTCAGCTACTTGGAACCAGGGAGTATCCGCCACATCTACCTGTACCATCACACACAGG GCCACAAGGCACTCTTTGGGGTCTTTATCCCCTCACAGCGAAGAGCATCTGTGTTTGTGTTGGATACTGT GCGCAGCAACCAAATGCCAGGGCTCAGTGCCCTATACTCAGCAGAACACAGCCTGCTGCTGGACAAGGTG GGCCCCAACCTCCTGCCTCCCCCAAAACACACCTTTGAAGTTCGTGCTGAAACCAACCTGAAGACCATCT GCAGAGCCATCCAACGCTTCCTGCTTGCCTACAAGGAAGAGCGCCGGGGGCCCACGCTCATCGCTATCCA GTCTAGCTGGGAACTGTGTAGGCTGACCAGTGAGATTCCAGTCTTAGAGGAGTTCCCACTAGTGCCTGTC CGAGTGGCTGACAAGATCAGCTATGCAGTCCTGGACTGGCAGCGCCACGGAGCTCGCCGAATGATCCGGC ACTACCTCAATTTAGACATTTGCCTTTCACAGGCCTTTGAGATGAGCAGGTACTTCCACATCCCCGTTGG AAACCTGCCAGAAGACATCTCCACCTTTGGCTCTGACCTCTTTTTTGCACGCCATCTCCAGCGCCATAAC CACCTACTTTGGTTGTCCCCTACCTCTCGGCCTGACCTGGGTGGGAAGGAAGCTGATGACAACCGCCTTG TCATGGAGTTTGATGACCGAGCCACTGTGGAGATCAATAATTCCGGCTGTTACTCTACTGTGTGCGTGGA GCTGGACATTCAAAACCTGGCAGTCAACACCATCCTCCAGTCCCACCATGTCAATGACATGGAGGGGGCT GGCAGCATGGGCATCAGCTTCGATGTGATCCAGCAGGCCTCCCTAGAGGACATGGTTACAGGCAATCAAG CTGCCAGTGCCCTGGCCAGCTACGATGAGACAGCCCTCTGCTCCAGTACGTTCAGGATCCTGAAGAGCAT GGTGGTTGGCTGGGTAAAGGAAATCACACAGTACCACAACATCTATGCTGACAACCAGGTGATGCACTTC TACCGCTGGCTCCAGTCGCCGTGCTCTCTGCTCCACGACCCAGCCCTTCACCGGACACTGCACAATATGA TGAAGAAGCTCTTCCTGCAGCTCATTGCTGAGTTCAAGCGCCTGGGGTCATCGGTCATCTATGCCAACTT CAATCGCATCATCCTCTGTACAAAGAAGCGCCGGATAGAGGATGCCCTTGCCTATGTGGAATATATCACC AACAGCATCCATTCTAAAGAGATCTTTCATTCCCTGACCATCTCTTTCTCTCGATGCTGGGAATTCCTTC TCTGGATGGATCCATCCAACTATGGTGGAATCAAAGGAAAAGTCCCATCCAGTATTCACTGTGGACAAGT AAGGGAGCAAGACTCCCAGACAAGGGAGGAAACTGAGGATGAGGAGGACAATGAAAAGGATGAGGAGGAA GAGGAGGACATGGGGCAATCTGAGGTTGAGGACTTACTGGAGAACAACTGGAACATTCTACAGTTCTTGC CACAGGCAGCCTCTTGCCAGAGCTACTTCCTTATGATCGTTTCAGCATACATCGTGGCTGTGTACCAAAG CATGAAGGATGAGTTGAGACACAGTGCCCCAGGCAGTACCCCTGTGAAGAGGAAGGGGGCCAGCCAGTTC TCCCAGGAGGCTGAAGGGGCAGCCGGGGCCCTTCCTGGAATGATCACTTTCTCTCAAGATTATGTGGCAA ATGAGCTCACTCAGACCTTCTTCACCATTACTCAGAAAATTCAGAAGAAAGTCACAGGCTCTCGGAACAC CGCTGAGCCCTCAGAGATGTTCCCTGTCCTCCCTGGTTCACATTTGCTGCTCAATAACCCTGCTCTGGAG TTCATCAAATACGTGTGCAAGGTACTATCTCTGGATACAAACATCACAAATCAGGTGAATAAGCTAAACA GAGACCTGCTTCGCCTGGTAGACGTTGGTGAATTCTCTGAGGAGGCCCAGTTCAGAGACCCCTGCCACTC CTACGTGCTCCCTGAGGTAATCTGCCACAGCTGTAATTTCTGCCGAGACCTGGACCTATGCAAAGATTCT TCTTTCTCTCAGGACGGAGCCATCCTGCCTCAGTGGCTTTGCTCCAATTGTCAGGCCCCCTACGACTCAT CTGCCATTGAGTCAGCCTTGGTGGAAGCCCTGCAGAGGAAACTGATGGCCTTCACACTTCAGGACCTGGT GTGCCTCAAGTGCCGTGGTATGAAAGAGACCCATATGCCTGTGTACTGCGGCTGCGCAGGGGACTTTGTT CTCACCATCCATACTGAGGTCTTCATGGAACAGATTAGAATCTTCCAGAACATTGCCAAGTACTACAACA TGTCATATCTCCAAGAGGCCATAGAATGGCTGCTACAGACAAGCCCTGTATCAAACTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR212034 representing NM_001107152 Red=Cloning site Green=Tags(s) MVLRNSGRRHPEPGADSEGSRDDGPSSSVSALKRLERSQWTDKMDLRFGFERLKEPGERTGWLINMHPTE ILDEDKRLVSAVDYYFIQDDGSRFKVALPYKPYFYIAARKGCDREVSSFLSKKFQGKIAKLENVPKEDLD LPNHLVGLKRSYIKLSFHTVEDLVKVRKEISPAVKKNREQDHASDEYTTMLSSILQGGSLITDEEETSKK IADQLDNIVDMREYDVPYHIRLSIDLRIHVAHWYNVRFRGNAFPVEIARRDDLVERPDPVVLAFDIETTK LPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCVFNEPDEVHLIQRWFEH IQETKPTIMVTYNGDFFDWPFVEARAAIHGLSMYQEIGFQKDSQGEYKAPQCIHMDCLRWVKRDSYLPVG SHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPD EVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTDDGHVLDAETYVGGHVEALESGVFRSDIPCRF RMNPAAFDFLLQRVEKTMRHAIEEEEKVPVEQATNFQEVCEQIKTKLTSLKDVPNRIECPLIYHLDVGAM YPNIILTNRLQPSAIVDEATCAACDFNKPGATCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLFP EGPARAFHELSREEQAKYEKRRLADYCRKAYKKIHMTKVEERLTTICQRENSFYVDTVRAFRDRRYEFKG LHKVWKKKLSAAVEVGDASEVKRCKNMEILYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGA NIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVIKTTNVKKPKLTISYPGAMLNIMVKEGFTNHQY QELTEPSSLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQ LIKIFQSSVFEAFLKGSTLEEVYASVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQK STSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWLKSSSLQ DFDIRTILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDIYKQKKISELFVLE GKRQIVMAQASENSLSLCTPDMEDFGLTKPYHSTVPVATKRKRVWESQKESQDITLTVPWQEVLGQPPSL GTTQEEWLVWLQFHKKKWQLQAQQRLARRKKQRLESAEDMPRLGAIREGPATGLGSFLRRTARSIMDLPW QIIQISETRQAGLFRLWAIIGSDLHCIKLSIPRVFYVNQRVAKAEDGPSYRKVNRALPRSNIVYNLYEYS VPEDMYQEHINEINTELSAPDIEGVYETQVPLLFRALVQLGCVCVVNKQLARQLSGWEAETFALEHLEMR SLAQFSYLEPGSIRHIYLYHHTQGHKALFGVFIPSQRRASVFVLDTVRSNQMPGLSALYSAEHSLLLDKV GPNLLPPPKHTFEVRAETNLKTICRAIQRFLLAYKEERRGPTLIAIQSSWELCRLTSEIPVLEEFPLVPV RVADKISYAVLDWQRHGARRMIRHYLNLDICLSQAFEMSRYFHIPVGNLPEDISTFGSDLFFARHLQRHN HLLWLSPTSRPDLGGKEADDNRLVMEFDDRATVEINNSGCYSTVCVELDIQNLAVNTILQSHHVNDMEGA GSMGISFDVIQQASLEDMVTGNQAASALASYDETALCSSTFRILKSMVVGWVKEITQYHNIYADNQVMHF YRWLQSPCSLLHDPALHRTLHNMMKKLFLQLIAEFKRLGSSVIYANFNRIILCTKKRRIEDALAYVEYIT NSIHSKEIFHSLTISFSRCWEFLLWMDPSNYGGIKGKVPSSIHCGQVREQDSQTREETEDEEDNEKDEEE EEDMGQSEVEDLLENNWNILQFLPQAASCQSYFLMIVSAYIVAVYQSMKDELRHSAPGSTPVKRKGASQF SQEAEGAAGALPGMITFSQDYVANELTQTFFTITQKIQKKVTGSRNTAEPSEMFPVLPGSHLLLNNPALE FIKYVCKVLSLDTNITNQVNKLNRDLLRLVDVGEFSEEAQFRDPCHSYVLPEVICHSCNFCRDLDLCKDS SFSQDGAILPQWLCSNCQAPYDSSAIESALVEALQRKLMAFTLQDLVCLKCRGMKETHMPVYCGCAGDFV LTIHTEVFMEQIRIFQNIAKYYNMSYLQEAIEWLLQTSPVSNF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107152
ORF Size	6849 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107152.2, NP_001100622.2
RefSeq Size	7101 bp
RefSeq ORF	6852 bp
Locus ID	304573
MW	262.1 kDa

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