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Dgkz (NM_031143) Rat Tagged ORF Clone
CAT＃: RR212478
- TrueORF®
Dgkz (Myc-DDK-tagged ORF) - Rat diacylglycerol kinase zeta (Dgkz), (10 ug)
"NM_031143" in other vectors (3)
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CNY 11,970.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR212478 representing NM_031143
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGCGGGACCCCAGCCCCGAGGCCCGGAGCAGCGACTCGGAGTCGGCCTCCGCCTCGTCCAGCG GCTCCGAGCGCGACGCGGATCCGGAGCCGGACAAGGCTCCGCGGCGCTTAACCAAGAGGCGCTTCCCGGG GCTGCGGCTCTTTGGGCACAGGAAAGCCATCACCAAGTCGGGCCTCCAGCACCTGGCACCCCCTCCTCCC ACGCCTGGGGCCCCGTGCGGTGAATCTGAGCGGCAGATCCGGAGTACTGTGGACTGGAGTGAGTCAGCAG CGTATGGGGAGCACATCTGGTTTGAGACCAACGTGTCCGGAGATTTCTGCTATGTCGGGGAGCAGTACTG TGTAGCTAAGATGCTGCCGAAGTCAGCGCCCAGAAGAAAGTGTGCAGCCTGTAAGATCGTGGTGCATACC CCTTGCATCGGGCAGCTGGAAAAGATCAATTTCCGCTGTAAGCCATCCTTCCGTGAATCAGGCTCCAGGA ATGTCCGTGAGCCAACCTTCGTAAGACACCACTGGGTCCACAGGCGACGCCAGGATGGCAAGTGTCGGCA CTGTGGGAAGGGCTTCCAGCAGAAGTTCACCTTCCACAGCAAAGAGATCGTAGCTATCAGCTGTTCCTGG TGCAAACAGGCGTACCACAGCAAGGTGTCCTGCTTCATGCTGCAGCAGATTGAGGAGCCCTGTTCCCTAG GGGTGCATGCAGCCGTGGTCATCCCACCCACCTGGATCCTACGGGCCCGGAGGCCCCAGAACACCCTCAA GGCCAGCAAGAAGAAAAAGAGAGCATCCTTCAAAAGGAGATCCAGTAAGAAAGGACCTGAGGAAGGCCGC TGGAGACCCTTCATCATCAGGCCCACCCCGTCCCCCCTCATGAAGCCCCTGCTAGTGTTTGTAAACCCCA AGAGTGGGGGTAACCAGGGCGCTAAGATCATCCAGTCTTTTCTGTGGTATCTGAACCCCCGGCAAGTCTT CGATCTGAGCCAGGGAGGACCCAGGGAAGCGCTGGAAATGTACCGCAAAGTCCATAATTTGAGGATTCTG GCTTGCGGGGGTGATGGCACGGTTGGCTGGATTCTTTCCACCCTAGACCAGCTGCGCTTAAAACCACCAC CTCCTGTGGCCATCCTGCCCCTGGGCACTGGCAATGACCTGGCCCGAACTCTCAACTGGGGTGGGGGTTA CACAGATGAGCCAGTGTCAAAGATCCTTTCCCATGTAGAGGAGGGGAACGTGGTACAGCTGGACCGTTGG GACCTCCGAGCAGAGCCCAACCCTGAGGCAGGGCCTGAGGAGCGAGATGACGGAGCCACTGACCGGCTGC CCCTGGATGTCTTCAACAACTACTTCAGTCTGGGCTTTGATGCCCACGTCACCCTGGAGTTTCATGAGTC TCGAGAAGCCAACCCAGAGAAGTTCAACAGCCGCTTTCGGAATAAGATGTTCTATGCTGGGACAGCCTTC TCTGACTTCCTGATGGGCAGCTCCAAGGACTTAGCCAAGCACATCCGAGTAGTGTGTGACGGAATGGACC TAACCCCCAAGATTCAGGACCTGAAACCGCAGTGCATAGTCTTTCTGAATATCCCCAGGTACTGTGCAGG CACCATGCCCTGGGGCCACCCTGGGGAGCACCATGACTTCGAGCCCCAGAGGCACGATGATGGCTACCTC GAGGTCATCGGCTTCACCATGACATCCTTGGCCGCATTGCAGGTGGGTGGACACGGCGAGCGGCTGACCC AGTGCCGAGAAGTGCTGCTCACCACTGCCAAGGCCATCCCTGTGCAGGTGGATGGTGAACCCTGCAAGCT TGCAGCATCGCGGATTCGAATTGCCCTGCGCAACCAGGCCACTATGGTGCAGAAGGCCAAGCGCCGGAGT ACTGCCCCACTGCACAGCGATCAGCAGCCAGTTCCTGAGCAGCTGAGGATCCAAGTGAGCAGGGTCAGCA TGCACGACTACGAGGCTCTCCATTATGACAAGGAGCAGCTCAAGGAGGCCTCTGTGCCTCTGGGTACTGT GGTGGTCCCCGGAGACAGTGACCTAGAGCTCTGCCGAGCCCACATTGAGAGACTCCAGCAGGAGCCCGAT GGGGCTGGAGCCAAGTCCCCCATGTGCCACCCACTGTCCTCCAAGTGGTGTTTCCTGGACGCCACCACTG CCAGCCGCTTCTACAGGATCGACAGGGCCCAGGAGCACCTCAACTATGTGACGGAGATTGCCCAGGATGA GATTTATATCCTAGACCCAGAGCTGCTGGGTGCATCAGCCCGACCTGACCTCCCTACCCCTACCTCCCCG CTCCCTGCCTCCCCCTGCTCCCCTACACCCGGGTCACTGCAGGGGGATGCCGCACTGCCCCAAGGTGAAG AGCTGATTGAAGCTGCCAAGAGGAATGACTTCTGCAAGCTCCAGGAACTACACCGAGCAGGAGGTGACCT CATGCACCGCGACCATCAGAGCCGCACACTCCTGCACCACGCAGTCAGCACTGGCAGTAAGGAAGTGGTC CGCTATCTGCTCGATCATGCACCACCAGAGATCCTCGATGCTGTGGAGGAGAACGGGGAGACCTGTCTAC ACCAGGCAGCCGCCCTAGGTCAGCGCACCATCTGCCACTACATTGTGGAAGCCGGGGCCTCCCTCATGAA GACAGATCAGCAGGGCGACACTCCCAGGCAGCGAGCTGAGAAGGCTCAAGACACAGAGCTAGCTGCATAC CTGGAGAACAGACAACATTACCAGATGATCCAACGTGAGGACCAGGAGACAGCTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR212478 representing NM_031143
Red=Cloning site Green=Tags(s) MEPRDPSPEARSSDSESASASSSGSERDADPEPDKAPRRLTKRRFPGLRLFGHRKAITKSGLQHLAPPPP TPGAPCGESERQIRSTVDWSESAAYGEHIWFETNVSGDFCYVGEQYCVAKMLPKSAPRRKCAACKIVVHT PCIGQLEKINFRCKPSFRESGSRNVREPTFVRHHWVHRRRQDGKCRHCGKGFQQKFTFHSKEIVAISCSW CKQAYHSKVSCFMLQQIEEPCSLGVHAAVVIPPTWILRARRPQNTLKASKKKKRASFKRRSSKKGPEEGR WRPFIIRPTPSPLMKPLLVFVNPKSGGNQGAKIIQSFLWYLNPRQVFDLSQGGPREALEMYRKVHNLRIL ACGGDGTVGWILSTLDQLRLKPPPPVAILPLGTGNDLARTLNWGGGYTDEPVSKILSHVEEGNVVQLDRW DLRAEPNPEAGPEERDDGATDRLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAF SDFLMGSSKDLAKHIRVVCDGMDLTPKIQDLKPQCIVFLNIPRYCAGTMPWGHPGEHHDFEPQRHDDGYL EVIGFTMTSLAALQVGGHGERLTQCREVLLTTAKAIPVQVDGEPCKLAASRIRIALRNQATMVQKAKRRS TAPLHSDQQPVPEQLRIQVSRVSMHDYEALHYDKEQLKEASVPLGTVVVPGDSDLELCRAHIERLQQEPD GAGAKSPMCHPLSSKWCFLDATTASRFYRIDRAQEHLNYVTEIAQDEIYILDPELLGASARPDLPTPTSP LPASPCSPTPGSLQGDAALPQGEELIEAAKRNDFCKLQELHRAGGDLMHRDHQSRTLLHHAVSTGSKEVV RYLLDHAPPEILDAVEENGETCLHQAAALGQRTICHYIVEAGASLMKTDQQGDTPRQRAEKAQDTELAAY LENRQHYQMIQREDQETAV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031143 |
| ORF Size | 2787 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031143.1, NP_112405.1 |
| RefSeq Size | 3560 bp |
| RefSeq ORF | 2790 bp |
| Locus ID | 81821 |
| UniProt ID | O08560 |
| MW | 104 kDa |
| Gene Summary | catalyzes the conversion of diacylglycerol to phosphatidic acid [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN212478 | Dgkz (untagged ORF) - Rat diacylglycerol kinase zeta (Dgkz), (10 ug) |
CNY 11,970.00 |
|
| RR212478L3 | Lenti ORF clone of Dgkz (Myc-DDK-tagged ORF) - Rat diacylglycerol kinase zeta (Dgkz), (10 ug) |
CNY 13,680.00 |
|
| RR212478L4 | Lenti ORF clone of Dgkz (mGFP-tagged ORF) - Rat diacylglycerol kinase zeta (Dgkz), (10 ug) |
CNY 15,010.00 |
