Abhd5 (NM_212524) Rat Tagged ORF Clone

CAT#: RR213964

  • TrueORF®

Abhd5 (Myc-DDK-tagged ORF) - Rat abhydrolase domain containing 5 (Abhd5), (10 ug)



  "NM_212524" in other vectors (3)

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CNY 3,990.00


货期*
5周

规格
    • 10 ug

Cited in 1 publication.

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经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms CGI-58
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR213964 representing NM_212524
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAAGCGATGGCGGCGGAGGAGGAGGTGGACTCGGCAGACGCCGGTGGAGGGTCAGGATGGCTGACAG
GGTGGCTTCCTACCTGGTGTCCCACGTCTACATCACACCTTAAAGAAGCTGAAGAGAAAATGCTAAAATG
TGTCCCCTGCACTTACAAGAAAGAACCTGTGCGCATATCCAATGGAAACAGCATATGGACACTGATGTTC
TCGCACAACATGTCTAGTAAGACACCACTTGTCCTCCTGCATGGTTTTGGAGGAGGTCTTGGACTTTGGG
CCCTGAATTTTGAAGATCTAAGCACTGATAGGCCTGTCTATGCCTTTGACCTATTGGGCTTTGGAAGAAG
TAGTAGACCTAGGTTTGACAGTGACGCGGAAGAAGTGGAGAATCAGTTTGTGGAATCCATTGAAGAGTGG
AGATGTGCCCTCAGGTTGGACAAAATGATCTTGCTTGGACACAACCTGGGAGGGTTCTTGGCTGCTGCCT
ACTCACTGAAGTACCCGTCAAGGGTCAGTCACCTCATTTTAGTAGAACCATGGGGTTTTCCTGAGCGACC
AGATCTTGCTGATCAAGAGAGACCAATTCCAGTTTGGATCAGAGCCTTAGGGGCAGCATTGACTCCCTTT
AACCCCTTGGCTGGCCTCAGGATTGCAGGACCTTTTGGGTTGAGTCTAGTGCAGCGCCTGAGGCCGGACT
TCAAGCGGAAGTACTCCTCTATGTTTGAAGATGACACGGTGACAGAGTACATCTACCACTGTAATGTACA
AACCCCAAGTGGTGAGACAGCTTTCAAAAACATGACGATTCCTTACGGGTGGGCCAAGCGGCCCATGCTC
CAGCGCATAGGTGGCTTGCACCCTGACATTCCAGTTTCAGTCATCTTCGGCGCCCGGTCCTGCATAGATG
GCAACTCTGGCACCAGCATCCAGTCACTGCGACCCAAGTCATACGTGAAGACAATTGCTATCCTCGGGGC
AGGGCATTATGTGTATGCAGACCAGCCAGAGGAGTTCAACCAGAAAGTCAAGGAGATCTGCCACACAGTA
GAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR213964 representing NM_212524
Red=Cloning site Green=Tags(s)

MKAMAAEEEVDSADAGGGSGWLTGWLPTWCPTSTSHLKEAEEKMLKCVPCTYKKEPVRISNGNSIWTLMF
SHNMSSKTPLVLLHGFGGGLGLWALNFEDLSTDRPVYAFDLLGFGRSSRPRFDSDAEEVENQFVESIEEW
RCALRLDKMILLGHNLGGFLAAAYSLKYPSRVSHLILVEPWGFPERPDLADQERPIPVWIRALGAALTPF
NPLAGLRIAGPFGLSLVQRLRPDFKRKYSSMFEDDTVTEYIYHCNVQTPSGETAFKNMTIPYGWAKRPML
QRIGGLHPDIPVSVIFGARSCIDGNSGTSIQSLRPKSYVKTIAILGAGHYVYADQPEEFNQKVKEICHTV
D

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_212524
ORF Size 1053 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_212524.1, NP_997689.1
RefSeq Size 1108 bp
RefSeq ORF 1056 bp
Locus ID 316122
UniProt ID Q6QA69
MW 39.1 kDa
Gene Summary Coenzyme A-dependent lysophosphatidic acid acyltransferase that catalyzes the transfert of an acyl group on a lysophosphatidic acid. Functions preferentially with 1-oleoyl-lysophosphatidic acid followed by 1-palmitoyl-lysophosphatidic acid, 1-stearoyl-lysophosphatidic acid and 1-arachidonoyl-lysophosphatidic acid as lipid acceptor. Functions preferentially with arachidonoyl-CoA followed by oleoyl-CoA as acyl group donors (By similarity). Functions in phosphatidic acid biosynthesis (By similarity). May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2 (By similarity). Involved in keratinocyte differentiation (By similarity). Regulates lipid droplet fusion (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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