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Dgki (NM_198782) Rat Tagged ORF Clone
CAT＃: RR214094
- TrueORF®
Dgki (Myc-DDK-tagged ORF) - Rat diacylglycerol kinase, iota (Dgki), (10 ug)
"NM_198782" in other vectors (3)
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CNY 16,820.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | rDGKi-3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR214094 representing NM_198782
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGCTGCGGGAAGGGGCTGCCATTTGCTGCCCCTGCCAGCGGCGCGCGGACCTGCCCGCGCTCCCG CCGCCTCCAGCGCCCTCAGCCCGGCCGGCCTCTGCAGCGGCACCGCCTCGGCTTCTTCCGCGGCCGCCGG AGCGGTCGCCATGAACCCCAGCTCCTCGGCGGGAGAGGAGAGAGGGGCGACGGGCGGTAGCAGCAGCAGC GGAAGCGGCGCCGGGAGCTGCTGCCTGGGCGCCGAGGGCGGCGTGGACCCGCGGGGTGCAGGGGCAGCGG CGGCTGCTGCCTTGGAGGAGCCCGCGGCCGCCGGACAGAAAGAGAAGGAAGAGGCGCTGGAGGAGAAGCT GAGGGACTTAACTTTCCGGAAGCAGGTCTCTTACAGGAAAGCCATCTCCCGGACAGGCCTCCAGCATCTG GCTCCTGCACATCCTCTTGGTCTTCCTGTGGCAAACGGTCCAGCGAAGGAGCCCAGAGCGACTCTGGACT GGAGTGAGAATGCTGTGAATGGGGAGCACCTATGGCTGGAGACCAACGTCTCGGGGGACCTCTGCTACTT GGGGGAGGAGAACTGCCAAGTCCGATTTGCAAAATCCGCTCTCAGGAGGAAGTGTGCTGTCTGCAAAATT GTGGTCCACACCGCCTGCATTGAGCAACTAGAGAAGATTAATTTCAGATGTAAACCTACATTTCGGGAAG GAGGCTCCAGATCGCCAAGAGAAAACTTTGTGCGCCATCACTGGGTCCACAGGAGGCGGCAGGAGGGAAA ATGTAAGCAGTGTGGTAAGGGCTTCCAGCAAAAGTTCTCCTTCCACAGTAAAGAGATCGTGGCCATCAGC TGTTCTTGGTGCAAGCAGGCGTTTCACAATAAAGTGACCTGCTTCATGCTGCATCACATCGAGGAACCCT GCTCCCTGGGGGCTCATGCTGCTGTTATTGTCCCGCCCACCTGGATCATTAAGGTGAAGAAACCTCAGAA CTCCCTGAAGGCTTCCAACCGGAAGAAGAAAAGAACAAGTTTTAAAAGGAAAGCCAGTAAAAGAGGAACC GAGCAGGAAAACAAAGGTCGTCCTTTTGTGATCAAGCCCATCTCCTCTCCTCTCATGAAGCCTTTGCTTG TCTTTGTGAACCCAAAGAGTGGGGGAAACCAGGGAACCAAAGTCCTACAGATGTTTATGTGGTACCTGAA TCCACGGCAAGTCTTTGACCTTTCTCAGGAAGGGCCAAAAGATGCGCTGGAGCTGTACAGGAAGGTACCA AATCTGCGAATTCTGGCCTGCGGTGGGGATGGAACGGTGGGCTGGATCCTCTCCATCCTGGACGAGCTGC AGCTGAGCCCACAGCCTCCTGTGGGGGTGCTTCCGCTGGGGACTGGGAACGACCTGGCTCGGACGCTCAA CTGGGGAGGGGGTTACACCGATGAGCCTGTTTCTAAGATCCTGTGCCAGGTGGAAGACGGGACCATTGTA CAGTTGGATCGCTGGAACCTCCATGTGGAGAGAAACCCAGACTTGCCACCTGAAGAACTTGAAGATGGCG TGTGTAAGCTTCCTCTGAATGTCTTCAATAATTACTTCAGCCTTGGATTTGACGCTCATGTCACATTGGA GTTCCATGAATCCCGAGAAGCAAATCCAGAGAAATTCAATAGTCGTTTTCGAAATAAAATGTTCTATGCG GGGGCAGCCTTTTCTGATTTCCTACAGAGAAGTTCTAGAGATCTATCCAAGCACGTCAAAGTTGTTTGTG ATGGGACAGACCTCACTCCAAAGATCCAGGACCTGAAGTTCCAGTGTATAGTATTTTTAAATATTCCCAG ATATTGTGCTGGCACAATGCCTTGGGGAAACCCTGGAGATCATCATGACTTTGAACCCCAGCGTCATGAT GATGGTTACATTGAAGTCATCGGATTCACTATGGCCTCTTTGGCTGCCCTGCAAGTCGGGGGCCACGGAG AAAGGTTGCACCAATGTCGGGAAGTGATGCTTCTGACGTACAAGTCCATTCCCATGCAAGTGGACGGGGA GCCATGTAGGTTGGCGCCTGCTATGATTCGGATCTCCTTGAGGAATCAGGCCAACATGGTACAGAAGAGC AAGCGGAGAACCTCAATGCCTTTGCTCAATGATCCCCAGTCTGTCCCAGATCGCCTGAGGATCCGCGTGA ACAAAATCAGTTTACAAGACTATGAAGGACTCCACTATGACAAGGAGAAACTCCGGGAAGCGTCCATCCC CCTGGGTATTCTAGTTGTTCGTGGAGACTGTGATTTGGAGACTTGCCGTATGTACATAGACCGCCTGCAG GAGGACTTGCAGTCAGTTTCTTCTGGGTCACAGAGAGTTCATTACCAGGACCAAGAAACTTCCTTCCCCA GGGCGATCTCAGCTCAGAGGCTGTCCCCACGGTGGTGCTTCCTAGACGCAACTTCTGCTGACCGCTTTTA TCGAATAGACAGATCTCAGGAACATTTGCACTTTGTGATGGAGATTTCTCATGATGAGATTTTTATTCTG GACCCAGATATGGTGGTGTCACAGCAGGCAGGGACACCTCCAGGAATGCCAGACCTAGTGGTTGAACAGG CTTCTGGGCTGTCTGACTGGTGGAACCCTGCCCTGAGGAAACGTATGCTGAGTGACAGTGGGATGATCAC ACCCCACTATGAGGACTCAGATCTGAAAGACTTCAGCCACTCCAGAGTGCTACAGTCACCAGTCTCTTCG GAAGATCATGCAATTCTGCAGGCAGTCATAACTGGTGACCTTATGAAGCTCATGGAAAGCTATAAGAATG GAGGGAGTTTATTAATTCAGGGACCCGGGCACTGTTCCCTCCTTCACTACGCAGCCAAGACTGGCAATGG GGAGATCGTGAAGTACATCCTTGACCATGGACCTGCGGAATTACTGGACATGGCAGACAGTGAAACGGGT GAGACTGCACTGCACAAGGCCGCCTGCCAAAGGAACCGGGCTGTATGCCAGCTTCTGGTGGACGCAGGAG CATCTCTGAGACAGACAGACTCCAAGGGAAAGACACCCCAAGAGCGAGCACAGCAGGCTGGGGACCCGGA TTTGGCTGCTTACCTAGAAAGCCGACAGAACTATAAGATCATTGGCCACGAAGACCTGGAAACTGCTGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR214094 representing NM_198782
Red=Cloning site Green=Tags(s) MDAAGRGCHLLPLPAARGPARAPAASSALSPAGLCSGTASASSAAAGAVAMNPSSSAGEERGATGGSSSS GSGAGSCCLGAEGGVDPRGAGAAAAAALEEPAAAGQKEKEEALEEKLRDLTFRKQVSYRKAISRTGLQHL APAHPLGLPVANGPAKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEENCQVRFAKSALRRKCAVCKI VVHTACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAIS CSWCKQAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGT EQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVP NLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTIV QLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYA GAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQDLKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHD DGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKS KRRTSMPLLNDPQSVPDRLRIRVNKISLQDYEGLHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQ EDLQSVSSGSQRVHYQDQETSFPRAISAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISHDEIFIL DPDMVVSQQAGTPPGMPDLVVEQASGLSDWWNPALRKRMLSDSGMITPHYEDSDLKDFSHSRVLQSPVSS EDHAILQAVITGDLMKLMESYKNGGSLLIQGPGHCSLLHYAAKTGNGEIVKYILDHGPAELLDMADSETG ETALHKAACQRNRAVCQLLVDAGASLRQTDSKGKTPQERAQQAGDPDLAAYLESRQNYKIIGHEDLETAV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_198782 |
| ORF Size | 3150 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_198782.2, NP_942077.2 |
| RefSeq Size | 3915 bp |
| RefSeq ORF | 3153 bp |
| Locus ID | 688705 |
| UniProt ID | F1MAB7 |
| MW | 115.9 kDa |
| Gene Summary | member of the family of diacylglyceride kinases which catalyze the phosphorylation of diacylglyceride to yield phosphatidic acid; involved in inositol lipid-mediated cell signaling [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN214094 | Dgki (untagged ORF) - Rat diacylglycerol kinase, iota (Dgki), (10 ug) |
CNY 16,820.00 |
|
| RR214094L3 | Lenti ORF clone of Dgki (Myc-DDK-tagged ORF) - Rat diacylglycerol kinase, iota (Dgki), (10 ug) |
CNY 18,530.00 |
|
| RR214094L4 | Lenti ORF clone of Dgki (mGFP-tagged ORF) - Rat diacylglycerol kinase, iota (Dgki), (10 ug) |
CNY 20,240.00 |
